Decline of vasopressin immunoreactivity and mRNA levels in the bed nucleus of the stria terminalis following castration
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Vasopressinergic (VP) neurons in the bed nucleus of the stria terminalis (BNST) of the rat are regulated by gonadal steroids. Gonadectomy causes the projections of the BNST to lose their VP immunoreactivity gradually over a period lasting more than 2 months. Here we have compared the rate of decline of VP mRNA and VP immunoreactivity in the BNST of adult male rats following castration. In experiment 1, the peak number of VP-immunoreactive cells and the level of VP gene expression were compared in sham-operated controls and at 1, 3, or 8 weeks postcastration. The number of VP-immunoreactive cells was not decreased at 1 week postcastration but was significantly reduced (p less than 0.0001) at 3 and 8 weeks postcastration. VP gene expression declined more rapidly, and both the total number of labeled cells (p less than 0.0001) and the average number of grains per cell (p less than 0.01) were significantly reduced by 1 week postcastration. No VP-expressing cells were detectable at 3 or 8 weeks. The difference in the rate of decline in the number of cells labeled by the two techniques following castration did not appear to be due to colchicine pretreatment. In experiment 2, VP mRNA in the BNST was compared in sham- operated controls or at 1, 3, or 7 d postcastration. A significant decrease (p less than 0.01) in the average number of grains per cell was detectable by just 1 d following castration, and the number of labeled cells was significantly reduced (p less than 0.001) by 3 d postcastration.(ABSTRACT TRUNCATED AT 250 WORDS)
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AVP mRNA is tightly regulated by testosterone.
“Given that AVP mRNA is tightly regulated by testosterone (Miller et al., 1989, 1992)”
From Sexually dimorphic role for vasopressin in the development of social play (2014) · cites this paper as Miller et al., 1992
The Discussion reports that vasopressin (VP) mRNA in the bed nucleus of the stria terminalis fell significantly within one day of castration, supporting strong sensitivity to gonadal steroid removal. The study does not isolate testosterone as the regulator, and its finding is specific to this brain region rather than AVP mRNA generally. (LLM, conf=0.86)
Experimental — accuracy not yet measuredMale rats castrated as adults show reduced AVP-ir in the MeP/pBNST→LS circuit as well as reduced levels of intermale aggression.
“A potential role of AVP in male aggression is largely based on the observation that male rats castrated as adults show reduced AVP-ir in the MeP/pBNST→LS circuit as well as reduced levels of intermale aggression (Koolhaas, Moor, Hiemstra, & Bohus, 1991; Miller et al., 1992; Szot & Dorsa, 1993; van Leeuwen et al., 1985).”
From Quantitative mapping reveals age and sex differences in vasopressin, but not oxytocin, immunoreactivity in the rat social behavior neural network (2017) · cites this paper as Miller et al., 1992
The paper reports that castration of adult male rats reduces vasopressin immunoreactivity in BNST neurons; its introduction also describes a postcastration loss of immunoreactive fibers in the septum, and the Discussion notes a similar, unquantified decline in the medial amygdala (page numbers unavailable). It does not report reduced intermale aggression, so that part of the claim is unsupported by this source. (LLM, conf=0.89)
Experimental — accuracy not yet measuredThese sex differences are maintained in adulthood by the actions of circulating gonadal hormones.
“Treating female or castrated male rat pups with testosterone increases the density of vasopressinergic inputs to the LS (De Vries et al., 1983), and these sex differences are maintained in adulthood by the actions of circulating gonadal hormones (Van Leeuwen et al., 1985; Miller et al., 1992).”
From Developmental origins of sex differences in the neural circuitry of play (2020) · cites this paper as Miller et al., 1992
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Cites
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Estradiol Modulates Density of Putative Oxytocin Receptors’ in Discrete Rat Brain Regions
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“They can modify synaptic inputs by in- Miller et al. * Decl i ne of VP lmmunoreactivity and VP mRNA i n BNST fluencing receptor density (Hammer, 1985; De Kloet et al., 1986; O'Connor et al., 1988) as well as transmitter biosynthesis (Blum et al., 1987; Argente et al., 1990; Chowen et al., 1990).”
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Dumais, K. M., & Veenema, A. H. (2016) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2015.04.003 · Cited by 520 · see citing works
“Taken together, across vertebrate species, VP synthesis and projections in the brain are often sexually dimorphic, VP within the BNST/MeA is modulated by gonadal hormones, and sex differences in VP may be sensitive to changes in status (dominant, subordinate), season, and photoperiod.”
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“The in situ hybridization closely followed a procedure originally developed by Dr. Scott Young (Young et al., 1986) and modified by Drs. Dan Dorsa and Margaret Miller (Miller et al., 1992).”
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Oxytocin and vasopressin: genetics and behavioral implications
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“LS circuit as well as reduced levels of intermale aggression (Koolhaas, Moor, Hiemstra, & Bohus, 1991; Miller et al., 1992; Szot & Dorsa, 1993; van Leeuwen et al., 1985).”
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Sexually dimorphic role for vasopressin in the development of social play
Paul, M. J., Terranova, J. I., Probst, C. K., Murray, E. K., Ismail, N. I., & de Vries, G. J. (2014) · Frontiers in Behavioral Neuroscience · doi:10.3389/fnbeh.2014.00058 · Cited by 51 · see citing works
“Given that AVP mRNA is tightly regulated by testosterone (Miller et al., 1989, 1992), this transient peak in BNST AVP mRNA is likely the result of activation from the neonatal surge in testosterone that occurs around birth (Szot and Dorsa, 1993 and references therein).”
BackgroundSelf-citationDevelopmental origins of sex differences in the neural circuitry of play
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Oxytocin, vasopressin and their interplay with gonadal steroids
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Arakawa, H., & Higuchi, Y. (2022) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2022.104597 · Cited by 8 · see citing works
- Authors
- Miller, M., DeVries, G., al-Shamma, H., & Dorsa, D.
- Affiliations
- United States Department of Veterans Affairs (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1992-08-01
- Volume
- 12
- Issue
- 8
- Pages
- 2881-2887
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6575668
- OpenAlex
- W2009365074
- Cited by
- 122
- References
- 4
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 2 · 2012: 1
- FWCI
- 15.79
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Digital Archive @ GSU (submittedVersion, cc-by-nc-sa)
- Funders
- National Science Foundation (8809799)
- U.S. Department of Veterans Affairs
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke (NS-20311, R01NS020311)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- vasopressin, bed nucleus of the stria terminalis, castration, vasopressin mRNA, gonadectomy, colchicine, vasopressinergic neurons
- MeSH terms
- Animals, Immunohistochemistry, Male, Nucleic Acid Hybridization, Orchiectomy, Rats, Inbred Strains, RNA, Messenger, Thalamic Nuclei, Vasopressins, Rats
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