CaMKIIβ Functions As an F-Actin Targeting Module that Localizes CaMKIIα/β Heterooligomers to Dendritic Spines
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Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine protein kinase that regulates long-term potentiation and other forms of neuronal plasticity. Functional differences between the neuronal CaMKIIalpha and CaMKIIbeta isoforms are not yet known. Here, we use green fluorescent protein-tagged (GFP-tagged) CaMKII isoforms and show that CaMKIIbeta is bound to F-actin in dendritic spines and cell cortex while CaMKIIalpha is largely a cytosolic enzyme. When expressed together, the two isoforms form large heterooligomers, and a small fraction of CaMKIIbeta is sufficient to dock the predominant CaMKIIalpha to the actin cytoskeleton. Thus, CaMKIIbeta functions as a targeting module that localizes a much larger number of CaMKIIalpha isozymes to synaptic and cytoskeletal sites of action.
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Inactive CaMKIIβ, but not α, binds with high affinity to F-actin.
“A second difference is the specific high-affinity binding of the inactive β, but not α, to F-actin (167–170).”
From Synaptic memory and CaMKII (2023) · cites this paper as [168]
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- Authors
- Shen, K., Teruel, M. N., Subramanian, K., & Meyer, T.
- Affiliations
- Duke Medical Center (US)
- Corresponding author
- Meyer, Tobias
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1998-09
- Volume
- 21
- Issue
- 3
- Pages
- 593-606
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2146831587
- Cited by
- 350
- References
- 61
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 5 · 2023: 9 · 2022: 9 · 2021: 6 · 2020: 20 · 2019: 14 · 2018: 18 · 2017: 10 · 2016: 7 · 2015: 12 · 2014: 9 · 2013: 21 · 2012: 14
- FWCI
- 5.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2r01gm051457-04)
- National Institute of General Medical Sciences (GM-48113, GM 51457)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- CaMKIIβ, CaMKIIα, CaMKII isoforms, hetero-oligomers, F-actin binding, dendritic spines, actin cytoskeleton, protein localization, synaptic plasticity, long-term potentiation, neuroplasticity
- MeSH terms
- Actins, Animals, Cloning, Molecular, Cytoskeleton, Dendrites, Isoenzymes, Luminescent Proteins, Nerve Fibers, Neurons, Phosphorylation, Recombinant Fusion Proteins, Synapses, Transcription, Genetic, Protein Biosynthesis, Tumor Cells, Cultured, 3T3 Cells, Calcium-Calmodulin-Dependent Protein Kinases, Macromolecular Substances, Green Fluorescent Proteins, Mice, Rats, Calcium-Calmodulin-Dependent Protein Kinase Type 2
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