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Cited by 257Neurogenesis and neuroplasticity mechanismsNeonatal and fetal brain pathologyNeuroinflammation and Neurodegeneration Mechanisms

Cell cycle dynamics of NG2 cells in the postnatal and ageing brain

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Psachoulia, Konstantina, Jamen, Francoise, Young, Kaylene M., Richardson, William D.·2009·Neuron Glia Biology
DOI 10.1017/s1740925x09990354PMC PMC6329448
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Abstract

Oligodendrocyte precursors (OLPs or 'NG2 cells') are abundant in the adult mouse brain, where they continue to proliferate and generate new myelinating oligodendrocytes. By cumulative BrdU labelling, we estimated the cell cycle time TC and the proportion of NG2 cells that is actively cycling (the growth fraction) at approximately postnatal day 6 (P6), P60, P240 and P540. In the corpus callosum, TC increased from <2 days at P6 to approximately 9 days at P60 to approximately 70 days at P240 and P540. In the cortex, TC increased from approximately 2 days to >150 days over the same period. The growth fraction remained relatively invariant at approximately 50% in both cortex and corpus callosum - that is, similar numbers of mitotically active and inactive NG2 cells co-exist at all ages. Our data imply that a stable population of quiescent NG2 cells appears before the end of the first postnatal week and persists throughout life. The mitotically active population acts as a source of new oligodendrocytes during adulthood, while the biological significance of the quiescent population remains to be determined. We found that the mitotic status of adult NG2 cells is unrelated to their developmental site of origin in the ventral or dorsal telencephalon. We also report that new oligodendrocytes continue to be formed at a slow rate from NG2 cells even after P240 (8 months of age).

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  • For every day after birth, TC in cortical gray matter increases by about two-thirds of a day, not one-third as previously estimated.

    “This remarkable conclusion still stands, although the rate of increase with time is greater than previously estimated: for every day after birth, TC in the cortical gray matter increases by about two-thirds of a day, not one-third as previously estimated (Psachoulia et al., 2009).”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Psachoulia et al., 2009

    The Discussion (page unavailable) reports cortical NG2-cell cycle time increasing from about 2 days at P6 to more than 150 days at P240, consistent with an average increase of roughly two-thirds of a day per day over that interval. The paper does not state that this rate holds for every day after birth or identify a previous one-third-of-a-day estimate. (LLM, conf=0.84)

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  • TC in cortical gray matter increases linearly with age from early postnatal ages until at least P540.

    “We reported previously that TC in the cortical gray matter increases linearly with age from early postnatal ages until at least P540 (Psachoulia et al., 2009).”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Psachoulia et al., 2009

    The Results section, “The NG2 cell cycle slows dramatically with age” (page unavailable), reports that cortical NG2-cell cycle time increased steadily from about 2 days at P6 to more than 100 days at P540. It does not establish a linear increase, and it says the P540 value cannot be determined confidently because the growth fraction is unknown. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Oligodendrocyte precursors (OPs) continue to proliferate and generate myelinating oligodendrocytes (OLs) well into adulthood.

    “Oligodendrocyte precursors (OPs) continue to proliferate and generate myelinating oligodendrocytes (OLs) well into adulthood.”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Psachoulia et al., 2009

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Authors
Psachoulia, K., Jamen, F., Young, K. M., & Richardson, W. D.
Affiliations
University College London (GB)
ORCID
Venue
Neuron Glia Biology
Publisher
Cambridge University Press (CUP)
Published
2009-11
Volume
5
Issue
3-4
Pages
57-67
ISSN
  • 1740-925X (ISSN-L)
  • 1741-0533
DOI
10.1017/s1740925x09990354
PMC
PMC6329448
OpenAlex
W2094997319
Cited by
257
References
59
Citations by year
2026: 9 · 2025: 14 · 2024: 8 · 2023: 12 · 2022: 17 · 2021: 20 · 2020: 23 · 2019: 11 · 2018: 15 · 2017: 28 · 2016: 19 · 2015: 19 · 2014: 17 · 2013: 17 · 2012: 9
FWCI
2.82
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuron Glia Biology (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • eCite Digital Repository (University of Tasmania) (submittedVersion)
  • UCL Discovery (University College London) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
  • Wellcome Trust
  • Alzheimer's Society
  • Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology
  • Medical Research Council
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and fetal brain pathology
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
oligodendrocyte precursor cells, cell cycle kinetics, cell cycle duration, growth fraction, postnatal brain development, brain aging, oligodendrocyte myelination, corpus callosum, cerebral cortex, telencephalon, adult neurogenesis, BrdU labeling
MeSH terms
Chondroitin Sulfate Proteoglycan 4, Age Factors, Aging, Animals, Antigens, Brain, Bromodeoxyuridine, Cell Count, Cell Cycle, Estrogen Antagonists, Luminescent Proteins, Mice, Transgenic, Oligodendroglia, Proteoglycans, Tamoxifen, Gene Expression Regulation, Developmental, Receptor, Platelet-Derived Growth Factor alpha, Mice

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