Reactions of the bioregulatory agent nitric oxide in oxygenated aqueous media: Determination of the kinetics for oxidation and nitrosation by intermediates generated in the nitric oxide/oxygen reaction
Open accessAbstract
The reaction kinetics of nitric oxide autoxidation in aerobic solutions were investigated by direct observation of the nitrite ion product and by trapping the strongly oxidizing and nitrosating intermediates formed in this reaction. The rate behavior observed for nitrite formation [rate = k3[O2][NO]2, k3 = (6 +/- 1.5) x 10(6) M-2 s-1 at 22 degrees C] was the same as found for oxidation of Fe(CN)6(4-) and of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and as for the nitrosation of sulfanilamide. There was a slight decrease in k3 to (3.5 +/- 0.7) x 10(6) M-2 s-1 at 37 degrees C. The second-order dependency for NO was observed at NO concentrations as low as 3 microM. The results of the competitive kinetics studies suggest that the key oxidizing intermediates, species which are both strong oxidants and nitrosating agents, are not one of those commonly proposed (NO2, N2O3, NO+, or O2NO-) but are one or more as yet uncharacterized NOx species.
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Studies have shown links between high NOX1 levels and hypertension, diabetes, ischemia/reperfusion injury, and coronary artery disease.
“Increased NOX1 activity is associated with vascular disease, and studies have shown links between high NOX1 levels and hypertension (1015), diabetes (163, 380, 1003), ischemia/reperfusion injury (479), and coronary artery disease (393).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [1015]
High nitric oxide concentrations are consumed quickly, while low concentrations can persist for minutes or even hours.
“High concentrations of NO will thus be quickly consumed, while low concentrations can persist for minutes or even hours [116, 118].”
From Signaling and stress: The redox landscape in NOS2 biology (2015) · cites this paper as [118]
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- Authors
- Wink, D. A., Darbyshire, J. F., Nims, R. W., Saavedra, J. E., & Ford, P. C.
- Affiliations
- Frederick National Laboratory for Cancer Research (US)
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 1993-01
- Volume
- 6
- Issue
- 1
- Pages
- 23-27
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W1990139307
- Cited by
- 480
- References
- 14
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 6 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 11 · 2016: 10 · 2015: 12 · 2014: 11 · 2013: 14 · 2012: 14
- FWCI
- 20.21
- Type
- journal article
- Language
- en
- Open access
- green
- escholarship.org
- Locations
- Chemical Research in Toxicology (publishedVersion)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Science Foundation (9024846)
- Division of Chemistry
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- nitric oxide, reaction kinetics, nitrite production, nitrosation, competition kinetics, nitric oxide dimer, nitric oxide cation, ABTS oxidation, ferricyanide oxidation, temperature dependence
- MeSH terms
- Aerobiosis, Diazonium Compounds, Ferrocyanides, Gases, Kinetics, Nitric Oxide, Nitroso Compounds, Oxidation-Reduction, Oxygen, Solutions, Spectrophotometry, Ultraviolet, Sulfanilamides, Sulfonic Acids, Benzothiazoles
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