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Three-dimensional segregation of supramolecular activation clusters in T cells

Monks, Colin R. F., Freiberg, Benjamin A., Kupfer, Hannah, Sciaky, Noah, Kupfer, Abraham·1998·Nature
DOI 10.1038/25764
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Abstract

Activation of T cells by antigen-presenting cells (APCs) depends on the complex integration of signals that are delivered by multiple antigen receptors. Most receptor-proximal activation events in T cells were identified using multivalent anti-receptor antibodies, eliminating the need to use the more complex APCs. As the physiological membrane-associated ligands on the APC and the activating antibodies probably trigger the same biochemical pathways, it is unknown why the antibodies, even at saturating concentrations, fail to trigger some of the physiological T-cell responses. Here we study, at the level of the single cell, the responses of T cells to native ligands. We used a digital imaging system and analysed the three-dimensional distribution of receptors and intracellular proteins that cluster at the contacts between T cells and APCs during antigen-specific interactions. Surprisingly, instead of showing uniform oligomerization, these proteins clustered into segregated three-dimensional domains within the cell contacts. The antigen-specific formation of these new, spatially segregated supramolecular activation clusters may generate appropriate physiological responses and may explain the high sensitivity of the T cells to antigen.

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Research works printed but not linked: 17

  1. Weiss, A. & Littman, D. R. Signal transduction by lymphocyte antigen receptors. Cell 76, 263–274 (1994). · DOI
  2. Qian, D. & Weiss, A. Tcell antigen receptor signal transduction. Curr. Opin. Cell Biol. 9, 205–212 (1997). · DOI
  3. Monks, C. R., Kupfer, H., Tamir, I., Barlow, A. & Kupfer, A. Selective modulation of protein kinase C-θ during T-cell activation. Nature 385, 83–86 (1997). · DOI
  4. Podack, E. R. & Kupfer, A. T-cell effector functions: mechanisms for delivery of cytotoxicity and help. Annu. Rev. Cell Biol. 7, 479–504 (1991). · DOI
  5. Singer, S. J. Intercellular communication and cell-cell adhesion. Science 255, 1671–1677 (1992). · DOI
  6. Kupfer, A. & Singer, S. J. Cell biology of cytotoxic and helper T cell functions: immunofluorescence microscopic studies of single cells and cell couples. Annu. Rev. Immunol. 7, 309–337 (1989). · DOI
  7. Hiraoka, Y., Sedat, J. W. & Agard, D. A. Determination of three-dimensional imaging properties of a light microscope system. Partial confocal behavior in epifluorescence microscopy. Biophys. J. 57, 325–333 (1990). · DOI
  8. Kupfer, A. & Singer, S. J. The specific interaction of helper T cells and antigen-presenting B cells. IV. Membrane and cytoskeletal reorganizations in the bound T cell as a function of antigen dose. J. Exp. Med. 170, 1697–1713 (1989). · DOI
  9. Kupfer, A., Burn, P. & Singer, S. J. The PMA-induced specific association of LFA-1 and talin in intact cloned T helper cells. J. Mol. Cell. Immunol. 4, 317–325 (1990).
  10. McCloskey, M. A. & Poo, M. M. Contact-induced redistribution of specific membrane components: local accumulation and development of adhesion. J. Cell Biol. 102, 2185–2196 (1986). · DOI
  11. Harding, C. V. & Unanue, E. R. Quantitation of antigen-presenting cell MHC class II/peptide complexes necessary for T-cell stimulation. Nature 346, 574–576 (1990). · DOI
  12. Anderson, M. S., Swier, K., Arneson, L. & Miller, J. Enhanced antigen presentation in the absence of the invariant chain endosomal localization signal. J. Exp. Med. 178, 1959–1969 (1993). · DOI
  13. Dittel, B. N., Sant'Angelo, D. B. & Janeway, C. A. J Peptide antagonists inhibit proliferation and the production of IL-4 and/or IFN-gamma in T helper 1, T helper 2, and T helper 0 clones bearing the same TCR. J. Immunol. 158, 4065–4073 (1997). · DOI
  14. Schlessinger, J. SH2/SH3 signaling proteins. Curr. Opin. Genet. Dev. 4, 25–30 (1994). · DOI
  15. Lemmon, M. A., Ferguson, K. M. & Schlessinger, J. PH domains: diverse sequences with a common fold recruit signaling molecules to the cell surface. Cell 85, 621–624 (1996). · DOI
  16. Pawson, T. & Scott, J. D. Signaling through scaffold, anchoring, and adaptor proteins. Science 278, 2075–2080 (1997). · DOI · Paper page
  17. Kupfer, H., Monks, C. R. & Kupfer, A. Small splenic B cells that bind to antigen-specific T helper (Th) cells and face the site of cytokine production in the Th cells selectively proliferate: immunofluorescence microscopic studies of Th-B-antigen presenting cell interactions. J. Exp. Med. 179, 1507–1515 (1994). · DOI

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Metadata

Authors
Monks, C. R. F., Freiberg, B. A., Kupfer, H., Sciaky, N., & Kupfer, A.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
1998-09
Volume
395
Issue
6697
Pages
82-86
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/25764
Cited by
1,968
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

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