Cited by 1,885SARS-CoV-2 and COVID-19 ResearchMonoclonal and Polyclonal Antibodies ResearchAnimal Virus Infections Studies

Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody

Open access
Pinto, Dora, Park, Young-Jun, Beltramello, Martina, Walls, Alexandra C., Tortorici, M. Alejandra, Bianchi, Siro, Jaconi, Stefano, Culap, Katja +17 more·2020·Nature

Published version of a preprint

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly emerged coronavirus that is responsible for the current pandemic of coronavirus disease 2019 (COVID-19), which has resulted in more than 3.7 million infections and 260,000 deaths as of 6 May 2020 1,2. Vaccine and therapeutic discovery efforts are paramount to curb the pandemic spread of this zoonotic virus. The SARS-CoV-2 spike (S) glycoprotein promotes entry into host cells and is the main target of neutralizing antibodies. Here we describe several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which we identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003. One antibody (named S309) potently neutralizes SARS-CoV-2 and SARS-CoV pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-binding domain of the S glycoprotein. Using cryo-electron microscopy and binding assays, we show that S309 recognizes an epitope containing a glycan that is conserved within the Sarbecovirus subgenus, without competing with receptor attachment. Antibody cocktails that include S309 in combination with other antibodies that we identified further enhanced SARS-CoV-2 neutralization, and may limit the emergence of neutralization-escape mutants. These results pave the way for using S309 and antibody cocktails containing S309 for prophylaxis in individuals at a high risk of exposure or as a post-exposure therapy to limit or treat severe disease.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41586-020-2349-y

Cites

87 of 87 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Several studies have shown that authentic virus and pseudovirus neutralization correlate quite well.

    “Several studies have now shown that authentic virus and pseudovirus neutralization correlate quite well (16, 86, 87).”
  • Serum neutralising activity is a correlate of protection for other respiratory viruses, including influenza and respiratory syncytial virus.

    “Serum neutralising activity is a correlate of protection for other respiratory viruses, including influenza35 and respiratory syncytial virus where prohylaxis with monoclonal antibodies has been used in at-risk groups36,37.”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Pinto, D., Park, Y.-J., Beltramello, M., Walls, A. C., Tortorici, M. A., Bianchi, S., Jaconi, S., Culap, K., Zatta, F., De Marco, A., Peter, A., Guarino, B., Spreafico, R., Cameroni, E., Case, J. B., Chen, R. E., Havenar-Daughton, C., Snell, G., Telenti, A., Virgin, H. W., Lanzavecchia, A., Diamond, M. S., Fink, K., Veesler, D., & Corti, D.
Affiliations
  • Vir Biotechnology (Switzerland) (CH)
  • University of Washington (US)
  • Centre National de la Recherche Scientifique (FR)
  • VIR Biotechnology (United States) (US)
  • Washington University in St. Louis (US)
Corresponding author
  • Veesler, David
  • Corti, Davide
Venue
Nature
Published
2020-05-18
Volume
583
Issue
7815
Pages
290-295
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W3025744748
Cited by
1,885
References
87
Citations by year
2026: 68 · 2025: 128 · 2024: 181 · 2023: 229 · 2022: 417 · 2021: 567 · 2020: 293
FWCI
49.43
Type
journal article
Language
en
Open access
Locations
  • Nature (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • U.S. Department of Energy (-AC02-05CH11231, Contract No. DE-AC02-05-CH11231, 05CH11231, No. DE-AC02-05-CH11231, DE AC02 05CH11231, DE-AC02-)
  • Burroughs Wellcome Fund (DE-AC02-05CH11231)
  • University of Washington
  • National Institutes of Health (1s10od023476-01)
  • Office of Science (No. DE-AC02- 05-CH11231, DEAC02–05CH11231, AC02-05CH11231, DE-AC02, Contract No. DE-AC02-05CH11231)
  • National Institute of General Medical Sciences (DE-AC02-05CH11231, R01GM120553)
  • National Institute of Allergy and Infectious Diseases (HHSN272201700059C, 75N93019C00062)
Topics
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
Keywords
SARS-CoV-2, COVID-19 pandemic, monoclonal antibodies, spike protein, receptor binding domain, cross-neutralization, SARS-CoV, memory B cells, antibody cocktail, neutralization escape mutants, post-exposure prophylaxis, therapeutic antibodies, carbohydrate epitopes
MeSH terms
Betacoronavirus, Angiotensin-Converting Enzyme 2, COVID-19, SARS-CoV-2, Animals, Antibodies, Monoclonal, Antibodies, Viral, Antibody-Dependent Cell Cytotoxicity, B-Lymphocytes, Chlorocebus aethiops, Cross Reactions, Humans, Immunoglobulin Fab Fragments, Immunologic Memory, Killer Cells, Natural, Peptidyl-Dipeptidase A, Models, Molecular, Neutralization Tests, Pneumonia, Viral, Vero Cells, Coronavirus Infections, Epitopes, B-Lymphocyte, Cryoelectron Microscopy, Severe Acute Respiratory Syndrome, Severe acute respiratory syndrome-related coronavirus, Immune Evasion, Antibodies, Neutralizing, HEK293 Cells, Pandemics, Spike Glycoprotein, Coronavirus

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review