Cited by 701Genomics and Phylogenetic StudiesBioinformatics and Genomic NetworksGene expression and cancer classification

Massive mining of publicly available RNA-seq data from human and mouse

Open access
Lachmann, Alexander, Torre, Denis, Keenan, Alexandra B., Jagodnik, Kathleen M., Lee, Hoyjin J., Wang, Lily, Silverstein, Moshe C., Ma’ayan, Avi·2018·Nature Communications

Published version of a preprint

Abstract

RNA sequencing (RNA-seq) is the leading technology for genome-wide transcript quantification. However, publicly available RNA-seq data is currently provided mostly in raw form, a significant barrier for global and integrative retrospective analyses. ARCHS4 is a web resource that makes the majority of published RNA-seq data from human and mouse available at the gene and transcript levels. For developing ARCHS4, available FASTQ files from RNA-seq experiments from the Gene Expression Omnibus (GEO) were aligned using a cloud-based infrastructure. In total 187,946 samples are accessible through ARCHS4 with 103,083 mouse and 84,863 human. Additionally, the ARCHS4 web interface provides intuitive exploration of the processed data through querying tools, interactive visualization, and gene pages that provide average expression across cell lines and tissues, top co-expressed genes for each gene, and predicted biological functions and protein–protein interactions for each gene based on prior knowledge combined with co-expression.

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  • RNA sequencing is an effective method for serial analysis of gene expression, which aids in understanding regulation mechanisms.

    “RNA sequencing (RNA-seq) is an effective method for serial analysis of gene expression, which aids in understanding regulation mechanisms (Lachmann et al., 2018).”

    From Multimodal MRI combined with RNA sequencing reveals pathological signatures in the 9-month-old 3×Tg-AD mouse brain (2026) · cites this paper as Lachmann et al., 2018

    The Introduction (page unavailable) describes RNA-seq as a common, sensitive method for quantifying gene expression. The Results section on predicting biological functions and protein interactions (page unavailable) uses expression data to predict upstream regulatory transcription factors, supporting a link to studying regulation. The paper does not describe serial or longitudinal analysis of gene expression. (LLM, conf=0.85)

    Experimental — accuracy not yet measured
  • Prior research has demonstrated that gene expression distribution can be mapped onto functional MRI.

    “Prior research has demonstrated that gene expression distribution can be mapped onto functional MRI (Lachmann et al., 2018).”

    From Multimodal MRI combined with RNA sequencing reveals pathological signatures in the 9-month-old 3×Tg-AD mouse brain (2026) · cites this paper as Lachmann et al., 2018

    The Discussion (page unavailable) says the resource calculates gene-expression distributions across tissues and cell lines. Our copy does not say those distributions were mapped onto functional MRI; searches for fMRI and functional magnetic resonance imaging found no passage addressing that link. (LLM, conf=0.76)

    Experimental — accuracy not yet measured
  • Age-similar, non-diseased, normal lung tissues were procured from lungs not used by the Northern California Transplant Donor Network.

    “Age-similar, non-diseased, normal lung tissues were procured from lungs not used by the Northern California Transplant Donor Network54.”

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Research works printed but not linked: 1

  1. [42]Folk, M., Heber, G., Koziol, Q., Pourmal, E. & Robinson, D. Proc. EDBT/ICDT 2011 Workshop on Array Databases (ACM, New York, NY, 2011).

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Authors
Lachmann, A., Torre, D., Keenan, A. B., Jagodnik, K. M., Lee, H. J., Wang, L., Silverstein, M. C., & Ma’ayan, A.
Affiliations
Icahn School of Medicine at Mount Sinai (US)
Corresponding author
Ma’ayan, Avi
Venue
Nature Communications
Published
2018-04-10
Volume
9
Issue
1
Pages
1366-1366
ISSN
2041-1723 (ISSN-L)
OpenAlex
W2953067954
Cited by
701
References
50
Citations by year
2026: 57 · 2025: 84 · 2024: 102 · 2023: 103 · 2022: 93 · 2021: 97 · 2020: 70 · 2019: 59 · 2018: 34 · 2017: 1
FWCI
23.11
Type
journal article
Language
en
Open access
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institutes of Health (UL1TR001433, OT3OD025467)
  • National Heart, Lung, and Blood Institute (U54HL127624, T32HL007824)
  • National Cancer Institute (U24CA224260, U54CA189201)
  • National Institute of General Medical Sciences (T32GM062754)
  • National Center for Advancing Translational Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
Keywords
Gene Expression Omnibus, human transcriptome, mouse transcriptome, gene co-expression, protein-protein interaction, gene function prediction, online resources
MeSH terms
Animals, Humans, Software, Genome, Internet, Protein Interaction Mapping, Databases, Genetic, Mice, Gene Regulatory Networks, Data Mining, Molecular Sequence Annotation, High-Throughput Nucleotide Sequencing, Transcriptome, Gene Ontology

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