Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging
Open accessAbstract
Significance: In vivo fluorescence imaging in near IR-IIb window (1,500–1,700 nm) can provide high spatial and temporal resolution and deep tissue penetration for fundamental research and potential translations. Herein, a bright fluorescent probe emitting at ∼1,600 nm based on lead sulfide (PbS)/CdS quantum dots was developed. The CdS shell helped to chemically passivate and retain the high fluorescence of the PbS core after phase transfer to aqueous solutions for biocompatibility. The 1,600-nm emitting probe allowed noninvasive, millimeter-deep fluorescence imaging at high speeds up to 60 frames per second with micrometer-scale spatial resolution in 2D wide-field and 3D confocal modes. The probes were nontoxic and largely excreted over 1 month, providing a tool for in vivo research of preclinical animal models.
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- Authors
- Zhang, M., Yue, J., Cui, R., Ma, Z., Wan, H., Wang, F., Zhu, S., Zhou, Y., Kuang, Y., Zhong, Y., Pang, D.-W., & Dai, H.
- Affiliations
- Wuhan University of Technology (CN)
- Stanford University (US)
- Wuhan University (CN)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2018-06-11
- Volume
- 115
- Issue
- 26
- Pages
- 6590-6595
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC6042152
- OpenAlex
- W2808317537
- Cited by
- 432
- References
- 61
- Citations by year
- 2026: 34 · 2025: 55 · 2024: 48 · 2023: 55 · 2022: 63 · 2021: 70 · 2020: 66 · 2019: 32 · 2018: 9
- FWCI
- 16.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- DH | National Institute for Health Research (DP1-NS-105737)
- Natural Science Foundation of Hubei Province (2015CFB401)
- China Scholarship Council (201606955059)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- PbS quantum dots, core-shell quantum dots, fluorescent probes, aqueous stability, amphiphilic polymer coating, blood circulation half-life, tumor vasculature imaging, enhanced permeability and retention effect, tumor accumulation, biliary excretion, deep tissue imaging, autofluorescence suppression
- MeSH terms
- Intravital Microscopy, Adenocarcinoma, Animals, Colonic Neoplasms, Drug Stability, Femoral Artery, Femoral Vein, Fluorescent Dyes, Half-Life, Hindlimb, Lead, Mice, Inbred C57BL, Microscopy, Electron, Microscopy, Fluorescence, Sulfides, Video Recording, Microscopy, Confocal, Imaging, Three-Dimensional, Quantum Dots, Mice, Optical Imaging
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