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CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)
Open accessAbstract
The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in multiple plant species. However, it remains unknown whether this system can be used for genome editing in grape. In this study, we described genome editing and targeted gene mutation in ‘Chardonnay’ suspension cells and plants via the CRISPR/Cas9 system. Two single guide RNAs (sgRNAs) were designed to target distinct sites of the L-idonate dehydrogenase gene (IdnDH). CEL I endonuclease assay and sequencing results revealed the expected indel mutations at the target site, and a mutation frequency of 100% was observed in the transgenic cell mass (CM) as well as corresponding regenerated plants with expression of sgRNA1/Cas9. The majority of the detected mutations in transgenic CM were 1-bp insertions, followed by 1- to 3-nucleotide deletions. Off-target activities were also evaluated by sequencing the potential off-target sites, and no obvious off-target events were detected. Our results demonstrated that the CRISPR/Cas9 system is an efficient and specific tool for precise genome editing in grape.
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla
The French–Italian Public Consortium for Grapevine Genome Characterization (2007) · Nature · doi:10.1038/nature06148 · Cited by 3,767
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Ran, F. A., Hsu, P. D., Lin, C.-Y., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., et al. (2013) · Cell · doi:10.1016/j.cell.2013.08.021 · Cited by 3,299
High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292 · cited 1×
“The probability of off-target events induced by the CRISPR/Cas9 system varies among different organisms 46,47 .”
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.04.007 · Cited by 2,369
Crystal structure of Cas9 in complex with guide RNA and target DNA
Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3117 · Cited by 2,268
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C., & Doudna, J. A. (2014) · Nature · doi:10.1038/nature13011 · Cited by 1,822
Targeted genome modification of crop plants using a CRISPR-Cas system
Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J. J., Qiu, J.-L., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2650 · Cited by 1,810
Self-citationRational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation
Doench, J. G., Hartenian, E., Graham, D. B., Tothova, Z., Hegde, M., Smith, I., Sullender, M., Ebert, B. L., Xavier, R. J., & Root, D. E. (2014) · Nature Biotechnology · doi:10.1038/nbt.3026 · Cited by 1,617
Yang, H., Wang, H., Shivalila, C. S., Cheng, A. W., Shi, L., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.08.022 · Cited by 1,596
Li, J.-F., Norville, J. E., Aach, J., McCormack, M., Zhang, D., Bush, J., Church, G. M., & Sheen, J. (2013) · Nature Biotechnology · doi:10.1038/nbt.2654 · Cited by 1,510
Double-Strand Break End Resection and Repair Pathway Choice
Symington, L. S., & Gautier, J. (2011) · Annual Review of Genetics · doi:10.1146/annurev-genet-110410-132435 · Cited by 1,493
Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna, J. A., & Liu, D. R. (2013) · Nature Biotechnology · doi:10.1038/nbt.2673 · Cited by 1,487
Jiang, W., Zhou, H., Bi, H., Fromm, M., Yang, B., & Weeks, D. P. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt780 · Cited by 1,247
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
Nekrasov, V., Staskawicz, B., Weigel, D., Jones, J. D. G., & Kamoun, S. (2013) · Nature Biotechnology · doi:10.1038/nbt.2655 · Cited by 1,127
Efficient genome editing in plants using a CRISPR/Cas system
Feng, Z., Zhang, B., Ding, W., Liu, X., Yang, D.-L., Wei, P., Cao, F., Zhu, S., Zhang, F., Mao, Y., et al. (2013) · Cell Research · doi:10.1038/cr.2013.114 · Cited by 1,073
Self-citationGenome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Wu, X., Scott, D. A., Kriz, A. J., Chiu, A. C., Hsu, P. D., Dadon, D. B., Cheng, A. W., Trevino, A. E., Konermann, S., Chen, S., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.2889 · Cited by 944
Targeted mutagenesis in rice using CRISPR-Cas system
Miao, J., Guo, D., Zhang, J., Huang, Q., Qin, G., Zhang, X., Wan, J., Gu, H., & Qu, L.-J. (2013) · Cell Research · doi:10.1038/cr.2013.123 · Cited by 913
CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants
Lei, Y., Lu, L., Liu, H.-Y., Li, S., Xing, F., & Chen, L.-L. (2014) · Molecular Plant · doi:10.1093/mp/ssu044 · Cited by 801
Self-citationZhang, H., Zhang, J., Wei, P., Zhang, B., Gou, F., Feng, Z., Mao, Y., Yang, L., Zhang, H., Xu, N., et al. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12200 · Cited by 792
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
Kuscu, C., Arslan, S., Singh, R., Thorpe, J., & Adli, M. (2014) · Nature Biotechnology · doi:10.1038/nbt.2916 · Cited by 777
Genome editing in rice and wheat using the CRISPR/Cas system
Shan, Q., Wang, Y., Li, J., & Gao, C. (2014) · Nature Protocols · doi:10.1038/nprot.2014.157 · Cited by 763
Self-citationBrooks, C., Nekrasov, V., Lippman, Z. B., & Van Eck, J. (2014) · PLANT PHYSIOLOGY · doi:10.1104/pp.114.247577 · Cited by 760
High-frequency modification of plant genes using engineered zinc-finger nucleases
Townsend, J. A., Wright, D. A., Winfrey, R. J., Fu, F., Maeder, M. L., Joung, J. K., & Voytas, D. F. (2009) · Nature · doi:10.1038/nature07845 · Cited by 737
Fauser, F., Schiml, S., & Puchta, H. (2014) · The Plant Journal · doi:10.1111/tpj.12554 · Cited by 713
Feng, Z., Mao, Y., Xu, N., Zhang, B., Wei, P., Yang, D.-L., Wang, Z., Zhang, Z., Zheng, R., Yang, L., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1400822111 · Cited by 657
Self-citationTargeted genome modifications in soybean with CRISPR/Cas9
Jacobs, T. B., LaFayette, P. R., Schmitz, R. J., & Parrott, W. A. (2015) · BMC Biotechnology · doi:10.1186/s12896-015-0131-2 · Cited by 577
Application of the CRISPR–Cas System for Efficient Genome Engineering in Plants
Mao, Y., Zhang, H., Xu, N., Zhang, B., Gou, F., & Zhu, J.-K. (2013) · Molecular Plant · doi:10.1093/mp/sst121 · Cited by 575
Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA
Jia, H., & Wang, N. (2014) · PLoS ONE · doi:10.1371/journal.pone.0093806 · Cited by 487
Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering
Zhang, Y., Zhang, F., Li, X., Baller, J. A., Qi, Y., Starker, C. G., Bogdanove, A. J., & Voytas, D. F. (2012) · Plant Physiology · doi:10.1104/pp.112.205179 · Cited by 486
Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation
Fan, D., Liu, T., Li, C., Jiao, B., Li, S., Hou, Y., & Luo, K. (2015) · Scientific Reports · doi:10.1038/srep12217 · Cited by 455
Self-citationPan, C., Ye, L., Qin, L., Liu, X., He, Y., Wang, J., Chen, L., & Lu, G. (2016) · Scientific Reports · doi:10.1038/srep24765 · Cited by 375
Self-citationTargeted mutagenesis in soybean using the CRISPR-Cas9 system
Sun, X., Hu, Z., Chen, R., Jiang, Q., Song, G., Zhang, H., & Xi, Y. (2015) · Scientific Reports · doi:10.1038/srep10342 · Cited by 371
Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants
Osakabe, Y., Watanabe, T., Sugano, S. S., Ueta, R., Ishihara, R., Shinozaki, K., & Osakabe, K. (2016) · Scientific Reports · doi:10.1038/srep26685 · Cited by 367
Xie, K., Zhang, J., & Yang, Y. (2014) · Molecular Plant · doi:10.1093/mp/ssu009 · Cited by 331
Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs
Shan, Q., Wang, Y., Chen, K., Liang, Z., Li, J., Zhang, Y., Zhang, K., Liu, J., Voytas, D. F., Zheng, X., et al. (2013) · Molecular Plant · doi:10.1093/mp/sss162 · Cited by 288
Self-citationXu, R.-F., Li, H., Qin, R.-Y., Li, J., Qiu, C.-H., Yang, Y.-C., Ma, H., Li, L., Wei, P.-C., & Yang, J.-B. (2015) · Scientific Reports · doi:10.1038/srep11491 · Cited by 227
l-Tartaric acid synthesis from vitamin C in higher plants
DeBolt, S., Cook, D. R., & Ford, C. M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510864103 · Cited by 213
Targeted genome modification technologies and their applications in crop improvements
Chen, K., & Gao, C. (2014) · Plant Cell Reports · doi:10.1007/s00299-013-1539-6 · Cited by 187
TALEN or Cas9 – Rapid, Efficient and Specific Choices for Genome Modifications
Wei, C., Liu, J., Yu, Z., Zhang, B., Gao, G., & Jiao, R. (2013) · Journal of Genetics and Genomics · doi:10.1016/j.jgg.2013.03.013 · Cited by 170
Ma, X., Chen, L., Zhu, Q., Chen, Y., & Liu, Y. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.02.012 · Cited by 155
Recent Progress in CRISPR/Cas9 Technology
Mei, Y., Wang, Y., Chen, H., Sun, Z. S., & Ju, X.-D. (2016) · Journal of Genetics and Genomics · doi:10.1016/j.jgg.2016.01.001 · Cited by 148
Self-citationIdentification of genomic sites for CRISPR/Cas9-based genome editing in the Vitis vinifera genome
Wang, Y., Liu, X., Ren, C., Zhong, G., Yang, L., Li, S., & Liang, Z. (2016) · BMC Plant Biology · doi:10.1186/s12870-016-0787-3 · Cited by 54
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Malnoy, M., Viola, R., Jung, M.-H., Koo, O.-J., Kim, S., Kim, J.-S., Velasco, R., & Nagamangala Kanchiswamy, C. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01904 · Cited by 756
CRISPR for Crop Improvement: An Update Review
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Wang, X., Tu, M., Wang, D., Liu, J., Li, Y., Li, Z., Wang, Y., & Wang, X. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12832 · Cited by 367
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Metadata
- Authors
- Ren, C., Liu, X., Zhang, Z., Wang, Y., Duan, W., Li, S., & Liang, Z.
- Venue
- Scientific Reports
- Published
- 2016-08-31
- Volume
- 6
- Issue
- 1
- Pages
- 32289-32289
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2516007520
- Cited by
- 300
- References
- 51
- FWCI
- 11.89
- Type
- journal article
- Language
- en
- Open access
- gold
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