A new method for sequencing DNA.

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Maxam, A M, Gilbert, W·1977·Proceedings of the National Academy of Sciences

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DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.

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Authors: Maxam, A. M., & Gilbert, W.
Venue: Proceedings of the National Academy of Sciences
Publisher: Proceedings of the National Academy of Sciences
Published: 1977-02
Volume: 74
Issue: 2
Pages: 560-564
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.74.2.560
OpenAlex: W1992595091
Cited by: 7,922
References: 0
FWCI: 201.72
Type: journal article
Language: en
Open access: green

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A new method for sequencing DNA.

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