An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides
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Lactating mothers secrete milk sialyloligosaccharides (MSOs) that function as anti-adhesives once provided to the neonate. Particular infant-associated commensals, such as Bifidobacterium longum subsp. infantis, consume neutral milk oligosaccharides, although their ability to utilize acidic oligosaccharides has not been assessed. Temporal glycoprofiling of acidic HMO consumed during fermentation demonstrated a single composition, with several isomers, corresponding to sialylated lacto-N-tetraose. To utilize MSO, B. longum subsp. infantis deploys a sialidase that cleaves α2-6 and α2-3 linkages. NanH2, encoded within the HMO catabolic cluster is up-regulated during HMO fermentation and is active on sialylated lacto-N-tetraose. These results demonstrate that commensal microorganisms do utilize MSO, a substrate that may be enriched in the distal gastrointestinal tract.
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Select strains of Bifidobacteria possess specialized enzymes capable of metabolizing human milk oligosaccharide glycans.
“Recent studies have shown that select strains of Bifidobacteria possess specialized enzymes capable of metabolizing MOS glycans15-17.”
From Diet and the Intestinal Microbiome: Associations, Functions, and Implications for Health and Disease (2014) · cites this paper as [17]
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Human Milk Hyaluronan Enhances Innate Defense of the Intestinal Epithelium
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A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate
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- Authors
- Sela, D. A., Li, Y., Lerno, L., Wu, S., Marcobal, A. M., German, J. B., Chen, X., Lebrilla, C. B., & Mills, D. A.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Mills, David A.
- ORCID
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 286
- Issue
- 14
- Pages
- 11909-11918
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC3069393
- OpenAlex
- W2008825357
- Cited by
- 192
- References
- 54
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 13 · 2023: 8 · 2022: 9 · 2021: 16 · 2020: 11 · 2019: 10 · 2018: 26 · 2017: 18 · 2016: 10 · 2015: 16 · 2014: 15 · 2013: 13 · 2012: 13
- FWCI
- 13.15
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture
- Dairy Management
- California Dairy Research Foundation
- National Institutes of Health
- Cooperative State Research, Education, and Extension Service
- National Institute of General Medical Sciences (R01GM076360, T32GM008799)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD065122, R01HD061923, R01HD059127)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- Bifidobacterium longum subsp. infantis, neuraminidase, human milk oligosaccharides, adhesion prevention, glycan profiling, infant gut microbiota
- MeSH terms
- Bacterial Proteins, Bifidobacterium, Humans, Milk, Human, Neuraminidase, Oligosaccharides, Mass Spectrometry
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