Cited by 194Infant Nutrition and HealthGut microbiota and healthMicrobial Metabolites in Food Biotechnology

An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides

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Sela, David A., Li, Yanhong, Lerno, Larry, Wu, Shuai, Marcobal, Angela M., German, J. Bruce, Chen, Xi, Lebrilla, Carlito B. +1 more·2011·Journal of Biological Chemistry

Abstract

Lactating mothers secrete milk sialyloligosaccharides (MSOs) that function as anti-adhesives once provided to the neonate. Particular infant-associated commensals, such as Bifidobacterium longum subsp. infantis, consume neutral milk oligosaccharides, although their ability to utilize acidic oligosaccharides has not been assessed. Temporal glycoprofiling of acidic HMO consumed during fermentation demonstrated a single composition, with several isomers, corresponding to sialylated lacto-N-tetraose. To utilize MSO, B. longum subsp. infantis deploys a sialidase that cleaves α2-6 and α2-3 linkages. NanH2, encoded within the HMO catabolic cluster is up-regulated during HMO fermentation and is active on sialylated lacto-N-tetraose. These results demonstrate that commensal microorganisms do utilize MSO, a substrate that may be enriched in the distal gastrointestinal tract.

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  1. Lewis, A. L., Desa, N., Hansen, E. E., Knirel, Y. A., Gordon, J. I., Gagneux, P., Nizet, V., and Varki, A. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 13552-13557
  2. Sela, D. A., Chapman, J., Adeuya, A., Kim, J. H., Chen, F., Whitehead, T. R., Lapidus, A., Rokhsar, D. S., Lebrilla, C. B., German, J. B., Price, N. P., Richardson, P. M., and Mills, D. A. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 18964 -18969

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Authors
Sela, D. A., Li, Y., Lerno, L., Wu, S., Marcobal, A. M., German, J. B., Chen, X., Lebrilla, C. B., & Mills, D. A.
Affiliations
University of California, Davis (US)
Corresponding author
Mills, David A.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2011-04
Volume
286
Issue
14
Pages
11909-11918
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2008825357
Cited by
192
References
54
Citations by year
2026: 5 · 2025: 4 · 2024: 13 · 2023: 8 · 2022: 9 · 2021: 16 · 2020: 11 · 2019: 10 · 2018: 26 · 2017: 18 · 2016: 10 · 2015: 16 · 2014: 15 · 2013: 13 · 2012: 13
FWCI
13.15
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Agriculture
  • Dairy Management
  • California Dairy Research Foundation
  • National Institutes of Health
  • Cooperative State Research, Education, and Extension Service
  • National Institute of General Medical Sciences (R01GM076360, T32GM008799)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD065122, R01HD061923, R01HD059127)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
Keywords
Bifidobacterium longum subsp. infantis, neuraminidase, human milk oligosaccharides, adhesion prevention, glycan profiling, infant gut microbiota
MeSH terms
Bacterial Proteins, Bifidobacterium, Humans, Milk, Human, Neuraminidase, Oligosaccharides, Mass Spectrometry

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