No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: Effects are independent of mitochondrial DNA integrity
Open accessAbstract
We investigated if calorie restriction (CR) preserved skeletal muscle oxidative capacity with aging after accounting for life span extension by CR, and determined if mitochondrial content, mitochondrial DNA integrity, and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) were involved. Ad libitum-fed (AL) and CR animals representing young adult, late middle age, and senescence were studied. Whereas citrate synthase and complex IV activities were lower in plantaris and gastrocnemius muscle of young adult CR animals, in contrast to the 15%-40% decline in senescent AL animals, there was no decline with aging in CR animals. There was no decline in citrate synthase protein in gastrocnemius with aging in either group, suggesting that CR preserves oxidative capacity with aging by protecting mitochondrial function rather than content. This protection was independent of mitochondrial DNA damage between groups. However, there was a slower decline in PGC-1alpha gene expression with aging in CR versus AL animals, suggesting a better maintenance of mitochondrial biogenesis with aging in CR animals.
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Normal ageing is associated with a trend for a decrease in PGC-1α expression.
“We found that normal ageing is associated with a trend for a decrease in PGC‐1α expression, which is a finding consistent with previous studies (Baker et al. 2006; Chabi et al. 2008; Joseph et al. 2012).”
From Parkin overexpression protects from ageing‐related loss of muscle mass and strength (2019) · cites this paper as Baker et al. 2006
Exercise training and calorie restriction are the most efficient interventions to attenuate sarcopenia.
“the most efficient interventions to attenuate sarcopenia are exercise training [21,22,23,24] and calorie restriction [25,26,27]”
From Mitochondrial Dynamics and Mitophagy in Skeletal Muscle Health and Aging (2021) · cites this paper as [25]
Calorie restriction attenuates sarcopenia in rodents.
“calorie restriction, one of the most efficient interventions to attenuate sarcopenia in rodents [25,26,27]”
From Mitochondrial Dynamics and Mitophagy in Skeletal Muscle Health and Aging (2021) · cites this paper as [25]
PGC-1α protein and its downstream molecular targets are reduced in aging animals and humans.
“PGC-1α protein and its downstream molecular targets are reduced in aging animals (Baker, Betik, Krause, and Hepple 2006) and humans.(Joseph, Adhihetty, Buford, Wohlgemuth, Lees, Nguyen, Aranda, Sandesara, Pahor, and Manini 2012;Safdar, Hamadeh, Kaczor, Raha, and Tarnopolsky 2010)”
From Successful aging: Advancing the science of physical independence in older adults (2015) · cites this paper as Baker et al. 2006
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- Authors
- Baker, D. J., Betik, A. C., Krause, D. J., & Hepple, R. T.
- Affiliations
- University of Calgary (CA)
- Corresponding author
- Baker, D. J.
- Venue
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-07-01
- Volume
- 61
- Issue
- 7
- Pages
- 675-684
- ISSN
- 1079-5006 (ISSN-L)
- 1758-535X
- OpenAlex
- W2151136828
- Cited by
- 89
- References
- 62
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 7 · 2016: 6 · 2015: 7 · 2014: 4 · 2013: 7 · 2012: 8
- FWCI
- 2.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journals of Gerontology Series A (publishedVersion)
- PubMed (publishedVersion)
- Victoria University Research Repository (Victoria University) (submittedVersion)
- Own your potential (DEAKIN) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- Canadian Institutes of Health Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- caloric restriction, skeletal muscle, oxidative capacity, aging, mitochondrial DNA integrity, PGC-1α, mitochondrial biogenesis, citrate synthase, cytochrome c oxidase, lifespan extension, gastrocnemius, plantaris, mitochondrial function
- MeSH terms
- Aging, Analysis of Variance, Animals, DNA, Mitochondrial, Male, Mitochondria, Muscle, Oxygen Consumption, Rats, Inbred F344, Muscle, Skeletal, Caloric Restriction, PPAR alpha, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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