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BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Open accessAbstract
We report the identities of the members of a group of proteins that associate with BRCA1 to form a large complex that we have named BASC (B RCA1-a ssociated genome s urveillance c omplex). This complex includes tumor suppressors and DNA damage repair proteins MSH2, MSH6, MLH1, ATM, BLM, and the RAD50–MRE11–NBS1 protein complex. In addition, DNA replication factor C (RFC), a protein complex that facilitates the loading of PCNA onto DNA, is also part of BASC. We find that BRCA1, the BLM helicase, and the RAD50–MRE11–NBS1 complex colocalize to large nuclear foci that contain PCNA when cells are treated with agents that interfere with DNA synthesis. The association of BRCA1 with MSH2 and MSH6, which are required for transcription-coupled repair, provides a possible explanation for the role of BRCA1 in this pathway. Strikingly, all members of this complex have roles in recognition of abnormal DNA structures or damaged DNA, suggesting that BASC may serve as a sensor for DNA damage. Several of these proteins also have roles in DNA replication-associated repair. Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair.
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“In addition to ATM, the members of the BASC include the mismatch repair proteins, MLH1, MSH2, and MSH6, the Bloom's syndrome helicase (BLM), and the Mre11-Rad50-NBS1 complex.”
From Cell cycle checkpoint signaling through the ATM and ATR kinases (2001) · cites this paper as Wang et al. 2000
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“Recent findings position ATM and ATR as critical upstream modulators of the breast cancer susceptibility protein, BRCA1”
From Cell cycle checkpoint signaling through the ATM and ATR kinases (2001) · cites this paper as Wang et al. 2000
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Metadata
- Authors
- Wang, Y., Cortez, D., Yazdi, P., Neff, N., Elledge, S. J., & Qin, J.
- Affiliations
- New York Blood Center (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2000-04-15
- Volume
- 14
- Issue
- 8
- Pages
- 927-939
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC316544
- OpenAlex
- W2127566231
- Cited by
- 1,221
- References
- 54
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 10 · 2023: 16 · 2022: 21 · 2021: 27 · 2020: 20 · 2019: 26 · 2018: 20 · 2017: 24 · 2016: 29 · 2015: 33 · 2014: 49 · 2013: 43 · 2012: 35
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Jane Coffin Childs Memorial Fund for Medical Research
- U.S. Bureau of Land Management
- U.S. Public Health Service (Q1187)
- National Institute of General Medical Sciences (GM-44664, R37GM044664, R01GM044664)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › BRCA gene mutations in cancer
- Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
- Keywords
- BRCA1, BASC, DNA repair, MSH2, MSH6, MLH1, ATM, BLM helicase, PCNA, DNA damage recognition, genome stability, tumor suppressor proteins, nuclear foci
- MeSH terms
- Adenosine Triphosphatases, Cell Cycle, Cell Nucleus, DNA Repair, DNA Helicases, DNA-Binding Proteins, Endodeoxyribonucleases, Exodeoxyribonucleases, Fluorescent Antibody Technique, Fungal Proteins, HeLa Cells, Humans, Nuclear Proteins, Precipitin Tests, Protein Binding, Mass Spectrometry, Transcription, Genetic, Signal Transduction, Cell Cycle Proteins, BRCA1 Protein, Saccharomyces cerevisiae Proteins, Replication Protein C, RecQ Helicases
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