Vitamin C Administration Attenuates Overload-Induced Skeletal Muscle Hypertrophy in Rats

Makanae, Y.; Kawada, S.; Sasaki, K.; Nakazato, K.; Ishii, N.

doi:10.1111/apha.12042

Cited by 59Muscle Physiology and DisordersExercise and Physiological ResponsesMuscle metabolism and nutrition

Vitamin C Administration Attenuates Overload-Induced Skeletal Muscle Hypertrophy in Rats

Makanae, Y., Kawada, S., Sasaki, K., Nakazato, K., Ishii, N.·2013·Acta Physiologica

DOI 10.1111/apha.12042

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Abstract

AIM: This study aimed to investigate the effects of vitamin C administration on skeletal muscle hypertrophy induced by mechanical overload in rats. METHODS: Male Wistar rats were randomly assigned to three groups: (i) sham-operated group (n = 8), (ii) placebo-administered group (n = 8) and (iii) vitamin C-administered group (n = 8). In the placebo-administered and vitamin C-administered groups, the gastrocnemius and soleus muscles of the right hindlimb were surgically removed to overload the plantaris muscle. Vitamin C (500 mg kg(-1)) was orally administered to the vitamin C-administered group once a day for 14 days. RESULTS: Synergist muscle ablation caused significant increases in wet weight and protein concentration of the plantaris muscle in both the placebo-administered (P < 0.01) and vitamin C-administered groups (P < 0.01) compared with the sham-operated group (SHA). However, the magnitude of plantaris muscle hypertrophy (expressed as a percentage of the contralateral plantaris muscle) was significantly smaller (P < 0.01) in the vitamin C-administered group (141%) than in the placebo-administered group (PLA) (152%). Compared with the SHA, only the PLA showed higher expressions of phosphorylated p70s6k and Erk1/2 (positive regulators of muscle protein synthesis) and a lower expression of atrogin-1 (a muscle atrophy marker). Concentrations of vitamin C and oxidative stress markers in the overloaded muscle were similar between the placebo-administered and vitamin C-administered groups. CONCLUSION: Oral vitamin C administration can attenuate overload-induced skeletal muscle hypertrophy, which may have implications for antioxidant supplementation during exercise training.

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In male Wistar rats, oral vitamin C administration can attenuate overload-induced skeletal muscle hypertrophy.
“In male Wistar rats, oral vitamin C administration can attenuate overload-induced skeletal muscle hypertrophy [57].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [57]

Increased oxidative stress in obesity and its impact on metabolic syndrome
Furukawa, S., Fujita, T., Shimabukuro, M., Iwaki, M., Yamada, Y., Nakajima, Y., Nakayama, O., Makishima, M., Matsuda, M., & Shimomura, I. (2004) · Journal of Clinical Investigation · doi:10.1172/jci21625 · Cited by 5,508 · see citing works
ROS as signalling molecules: Mechanisms that generate specificity in ROS homeostasis
D'Autréaux, B., & Toledano, M. B. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2256 · Cited by 3,642 · see citing works
Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo
Bodine, S. C., Stitt, T. N., Gonzalez, M., Kline, W. O., Stover, G. L., Bauerlein, R., Zlotchenko, E., Scrimgeour, A., Lawrence, J. C., Glass, D. J., et al. (2001) · Nature Cell Biology · doi:10.1038/ncb1101-1014 · Cited by 2,752 · see citing works
Antioxidant enzymes and human diseases
MatÉs, J. M., Pérez-Gómez, C., & De Castro, I. N. (1999) · Clinical Biochemistry · doi:10.1016/s0009-9120(99)00075-2 · Cited by 2,099 · see citing works
Antioxidants prevent health-promoting effects of physical exercise in humans
Ristow, M., Zarse, K., Oberbach, A., Klöting, N., Birringer, M., Kiehntopf, M., Stumvoll, M., Kahn, C. R., & Blüher, M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903485106 · Cited by 1,582 · see citing works
Oxidative stress and cancer: Have we moved forward?
Halliwell, B. (2007) · Biochemical Journal · doi:10.1042/bj20061131 · Cited by 1,302 · see citing works
Oral administration of vitamin C decreases muscle mitochondrial biogenesis and hampers training-induced adaptations in endurance performance
Gomez-Cabrera, M.-C., Domenech, E., Romagnoli, M., Arduini, A., Borras, C., Pallardo, F. V., Sastre, J., & Viña, J. (2008) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/87.1.142 · Cited by 844 · see citing works
Phosphorylation of p70<sup>S6k</sup>correlates with increased skeletal muscle mass following resistance exercise
Baar, K., & Esser, K. (1999) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1999.276.1.c120 · Cited by 726 · see citing works
Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle
Dreyer, H. C., Fujita, S., Cadenas, J. G., Chinkes, D. L., Volpi, E., & Rasmussen, B. B. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.113175 · Cited by 570 · see citing works
Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle
Louis, E., Raue, U., Yang, Y., Jemiolo, B., & Trappe, S. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00679.2007 · Cited by 478 · see citing works
Reactive Oxygen Species Are Signalling Molecules for Skeletal Muscle Adaptation
Powers, S. K., Duarte, J., Kavazis, A. N., & Talbert, E. E. (2010) · Experimental Physiology · doi:10.1113/expphysiol.2009.050526 · Cited by 440 · see citing works
EFFECTS OF ACUTE AEROBIC AND ANAEROBIC EXERCISE ON BLOOD MARKERS OF OXIDATIVE STRESS
Bloomer, R. J., Goldfarb, A. H., Wideman, L., MCKENZIE, M. J., & Consitt, L. A. (2005) · The Journal of Strength and Conditioning Research · doi:10.1519/14823.1 · Cited by 328 · see citing works
The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy
Adams, G. R., & Haddad, F. (1996) · Journal of Applied Physiology · doi:10.1152/jappl.1996.81.6.2509 · Cited by 287 · see citing works
Nox2 redox signaling maintains essential cell populations in the brain
Dickinson, B. C., Peltier, J., Stone, D., Schaffer, D. V., & Chang, C. J. (2010) · Nature Chemical Biology · doi:10.1038/nchembio.497 · Cited by 273 · see citing works
The involvement of the ubiquitin proteasome system in human skeletal muscle remodelling and atrophy
Murton, A. J., Constantin, D., & Greenhaff, P. L. (2008) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2008.10.011 · Cited by 265 · see citing works
Resistance exercise-induced increase in muscle mass correlates with p70S6 kinase phosphorylation in human subjects
Terzis, G., Georgiadis, G., Stratakos, G., Vogiatzis, I., Kavouras, S., Manta, P., Mascher, H., & Blomstrand, E. (2007) · European Journal of Applied Physiology · doi:10.1007/s00421-007-0564-y · Cited by 242 · cited 1× · see citing works
“Erk1/2 and p70s6k, both of which act as a mediator of skeletal muscle hypertrophy (Baar & Esser 1999, Haddad & Adams 2004, Shi et al. 2007, Terzis et al. 2008), are shown to be phosphorylated by mechanical overload (Carlson et al. 2001, Miyazaki et al. 2011).”
Background
Work-induced growth of skeletal muscle in normal and hypophysectomized rats
Goldberg, A. (1967) · American Journal of Physiology-Legacy Content · doi:10.1152/ajplegacy.1967.213.5.1193 · Cited by 218 · see citing works
Resistance Exercise Increases Muscle Protein Synthesis and Translation of Eukaryotic Initiation Factor 2Bϵ mRNA in a Mammalian Target of Rapamycin-dependent Manner
Kubica, N., Bolster, D. R., Farrell, P. A., Kimball, S. R., & Jefferson, L. S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m413732200 · Cited by 207 · see citing works
MDA content increases in fast- and slow-twitch skeletal muscle with intensity of exercise in a rat
Alessio, H. M., Goldfarb, A. H., & Cutler, R. G. (1988) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1988.255.6.c874 · Cited by 206 · see citing works
Early activation of mTORC1 signalling in response to mechanical overload is independent of phosphoinositide 3‐kinase/Akt signalling
Miyazaki, M., McCarthy, J. J., Fedele, M. J., & Esser, K. A. (2011) · The Journal of Physiology · doi:10.1113/jphysiol.2011.205658 · Cited by 194 · see citing works
Endurance exercise results in DNA damage as detected by the comet assay
Mastaloudis, A., Yu, T., O'Donnell, R. P., Frei, B. B., Dashwood, R. H., & Traber, M. G. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.01.012 · Cited by 153 · see citing works
Sex-based comparisons of myofibrillar protein synthesis after resistance exercise in the fed state
West, D. W. D., Burd, N. A., Churchward-Venne, T. A., Camera, D. M., Mitchell, C. J., Baker, S. K., Hawley, J. A., Coffey, V. G., & Phillips, S. M. (2012) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00170.2012 · Cited by 143 · see citing works
Inhibition of MAP/ERK kinase prevents IGF-I-induced hypertrophy in rat muscles.
Haddad, F., & Adams, G. (2004) · Journal of applied physiology · doi:10.1152/japplphysiol.00856.2003 · Cited by 139 · see citing works
Myogenic and proteolytic mRNA expression following blood flow restricted exercise
Manini, T. M., Vincent, K. R., Leeuwenburgh, C., Lees, H. A., Kavazis, A. N., Borst, S. E., & Clark, B. C. (2010) · Acta Physiologica · doi:10.1111/j.1748-1716.2010.02172.x · Cited by 117 · see citing works
Ibuprofen inhibits skeletal muscle hypertrophy in rats.
Soltow, Q. A., Betters, J., Sellman, J., Lira, V., Long, J. H. D., & Criswell, D. (2006) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000218142.98704.66 · Cited by 117 · see citing works
Skeletal Muscle Mass Recovery from Atrophy in IL-6 Knockout Mice
Washington, T. A., White, J., Davis, J. M., Wilson, L., Lowe, L. L., Sato, S., & Carson, J. A. (2011) · Acta Physiologica · doi:10.1111/j.1748-1716.2011.02281.x · Cited by 106 · see citing works
Reactive Oxygen Species Play an Essential Role in IGF-I Signaling and IGF-I-Induced Myocyte Hypertrophy in C2C12 Myocytes
Handayaningsih, A. E., Iguchi, G., Fukuoka, H., Nishizawa, H., Takahashi, M., Yamamoto, M., Herningtyas, E. H., Okimura, Y., Kaji, H., Chihara, K., et al. (2011) · Endocrinology · doi:10.1210/en.2010-0981 · Cited by 97 · see citing works
Role of active oxygen species and antioxidants in photoaging
Yamamoto, Y. (2001) · Journal of Dermatological Science · doi:10.1016/s0923-1811(01)00120-7 · Cited by 93 · see citing works
COX-2 inhibitor reduces skeletal muscle hypertrophy in mice
Novak, M. L., Billich, W., Smith, S. M., Sukhija, K. B., McLoughlin, T. J., Hornberger, T. A., & Koh, T. J. (2009) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.90874.2008 · Cited by 92 · see citing works
THE METABOLISM OF ASCORBIC ACID-1-C14 AND OXALIC ACID-C14 IN THE RAT
Curtin, C. O., & KING, C. G. (1955) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)81411-4 · Cited by 88 · see citing works
Effect of antioxidant supplementation on insulin sensitivity in response to endurance exercise training
Yfanti, C., Nielsen, A. R., Åkerström, T., Nielsen, S., Rose, A. J., Richter, E. A., Lykkesfeldt, J., Fischer, C. P., & Pedersen, B. K. (2011) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00207.2010 · Cited by 85 · see citing works
Selenoprotein-Deficient Transgenic Mice Exhibit Enhanced Exercise-Induced Muscle Growth
Hornberger, T. A., McLoughlin, T. J., Leszczynski, J. K., Armstrong, D., Esser, K. A., Jameson, R. R., Bowen, P. E., Hwang, E., Hou, H., Moustafa, M. E., et al. (2003) · Journal of Nutrition · doi:10.1093/jn/133.10.3091 · Cited by 80 · see citing works
Metabolic character of hypertrophied rat muscle
Ianuzzo, C. D., & CHEN, V. (1979) · Journal of Applied Physiology · doi:10.1152/jappl.1979.46.4.738 · Cited by 74 · see citing works
Vitamin C or Vitamin B6 supplementation prevent the oxidative stress and decrease of prostacyclin generation in homocysteinemic rats
Mahfouz, M., & Kummerow, F. A. (2004) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2004.01.028 · Cited by 66 · see citing works
Extracellular signal-regulated kinase pathway is differentially involved in β-agonist-induced hypertrophy in slow and fast muscles
Shi, H., Zeng, C., Ricome, A., Hannon, K., Grant, A. L., & Gerrard, D. E. (2006) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00466.2006 · Cited by 66 · see citing works
Time course of the MAPK and PI3-kinase response within 24 h of skeletal muscle overload
Carlson, C. J., Fan, Z., Gordon, S. E., & Booth, F. W. (2001) · Journal of Applied Physiology · doi:10.1152/jappl.2001.91.5.2079 · Cited by 59 · see citing works
Vitamin C homeostasis in skeletal muscle cells
Savini, I., Catani, M. V., Duranti, G., Ceci, R., Sabatini, S., & Avigliano, L. (2005) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.12.009 · Cited by 57 · see citing works
Redox regulation of vitamin C transporter SVCT2 in C2C12 myotubes
Savini, I., Rossi, A., Catani, M. V., Ceci, R., & Avigliano, L. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.07.007 · Cited by 52 · see citing works
High-dose antioxidant vitamin C supplementation does not prevent acute exercise-induced increases in markers of skeletal muscle mitochondrial biogenesis in rats
Wadley, G. D., & McConell, G. K. (2010) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00127.2010 · Cited by 49 · see citing works
Atrogin-1 Affects Muscle Protein Synthesis and Degradation When Energy Metabolism Is Impaired by the Antidiabetes Drug Berberine
Wang, H., Liu, D., Cao, P., Lecker, S. H., & Hu, Z. (2010) · Diabetes · doi:10.2337/db10-0207 · Cited by 48 · see citing works
Heat shock protein accumulation and heat shock transcription factor activation in rat skeletal muscle during compensatory hypertrophy
Locke, M. (2007) · Acta Physiologica · doi:10.1111/j.1748-1716.2007.01764.x · Cited by 30 · see citing works

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Pileggi, C. A., Hedges, C. P., D’Souza, R. F., Durainayagam, B. R., Markworth, J. F., Hickey, A. J., Mitchell, C. J., & Cameron‐Smith, D. (2018) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2018.06.012 · Cited by 15 · see citing works
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Martínez‐Ferrán, M., Berlanga, L. A., Guido, O. B., Duarte, M. M., Vicente-Campos, D., Sánchez-Jorge, S., Morales, C. R., Munguía‐Izquierdo, D., & Pareja-Galeano, H. (2022) · Nutrition · doi:10.1016/j.nut.2022.111848 · Cited by 14 · see citing works
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Pastor, R., & Tur, J. A. (2019) · Biochemical Pharmacology · doi:10.1016/j.bcp.2019.113649 · Cited by 13 · see citing works
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Kent, E., Coleman, S. J., Bruemmer, J. E., Casagrande, R. R., Levihn, C., Romo, G., Herkelman, K. L., & Hess, T. M. (2022) · Journal of Equine Veterinary Science · doi:10.1016/j.jevs.2022.104200 · Cited by 10 · see citing works
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Persson, P. B. (2015) · Acta Physiologica · doi:10.1111/apha.12451 · Cited by 10 · see citing works
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Takamura, Y., Makanae, Y., Ato, S., Yoshii, N., Kido, K., Nomura, M., Uchiyama, A., Shiozawa, N., & Fujita, S. (2016) · Nutrition Research · doi:10.1016/j.nutres.2016.09.002 · Cited by 10 · see citing works
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Horii, M., Bumrungkit, C., Yanaka, N., Hawke, T. J., Rebalka, I. A., & Kumrungsee, T. (2025) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00963.2024 · Cited by 7 · see citing works
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Lu, S., Zhou, Y., Liu, M., Gong, L., Liu, L., Duan, Z., Chen, K., Gonzalez, F. J., Wei, F., Xiang, R., et al. (2024) · Antioxidants and Redox Signaling · doi:10.1089/ars.2024.0595 · Cited by 5 · see citing works
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Tokuda, N., Watanabe, D., Naito, A., Yamauchi, N., Ashida, Y., Cheng, A. J., & Yamada, T. (2023) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00127.2023 · Cited by 3 · see citing works
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Persson, A. B., & Persson, P. B. (2013) · Acta Physiologica · doi:10.1111/apha.12193
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Kudrna, R. A. (2014) · KU ScholarWorks (The University of Kansas)
Efeitos da suplementação com glutamina e/ou do treinamento físico resistido nas vias de sinalização da síntese e degradação de proteínas no músculo esquelético de ratos.
Rodrigues, C. (2015) · doi:10.11606/t.42.2015.tde-07122015-184843
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Cristina, T., & Curi, P. (2015)
Heat shock proteins and endogenous antioxidants in skeletal muscle: acute responses to exercise and adaptations to training
Cumming, K. T. (2014) · BIBSYS Brage (BIBSYS (Norway))
Regulation of antioxidant defenses in the prevention of skeletal muscle deconditioning
Arc‐Chagnaud, C. (2019) · doi:10.70675/5f87ccb5z5cb5z4962zb4c3z4133c1b52a4b

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Paulsen, G., Hamarsland, H., Cumming, K. T., Johansen, R. E., Hulmi, J. J., Børsheim, E., Wiig, H., Garthe, I., & Raastad, T. (2014) · The Journal of Physiology · doi:10.1113/jphysiol.2014.279950 · Cited by 148 · see citing works
High-dose antioxidant vitamin C supplementation does not prevent acute exercise-induced increases in markers of skeletal muscle mitochondrial biogenesis in rats
Wadley, G. D., & McConell, G. K. (2010) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00127.2010 · Cited by 49 · see citing works
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Kitakaze, T., Nakatsuji, A., Harada, N., & Yamaji, R. (2026) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.72.176
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Yada, K., Matoba, H., & Kitaura, T. (2011) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000401202.37466.31 · Cited by 1 · see citing works

Authors: Makanae, Y., Kawada, S., Sasaki, K., Nakazato, K., & Ishii, N.
Affiliations: The University of Tokyo (JP)
Japan Women's University (JP)
Nippon Sport Science University (JP)
Corresponding author: Makanae, Y.
ORCID: Makanae, Y.
Sasaki, K.
Nakazato, K.
Venue: Acta Physiologica
Publisher: Wiley
Published: 2013-01-28
Volume: 208
Issue: 1
Pages: 57-65
ISSN: 1748-1708 (ISSN-L)
1748-1716
DOI: 10.1111/apha.12042
OpenAlex: W2032922626
Cited by: 59
References: 41
Citations by year: 2025: 2 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 9 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 9 · 2014: 6 · 2013: 2
FWCI: 2.19
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Acta Physiologica (publishedVersion)
PubMed (publishedVersion)
Institutional Repositories DataBase (IRDB) (submittedVersion)
Funders: Japan Society for the Promotion of Science (22300210, 24300221)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords: vitamin C, skeletal muscle hypertrophy, mechanical overload, plantaris muscle, p70S6K, ERK1/2, atrogin-1, muscle protein synthesis, oxidative stress, antioxidant supplementation, exercise training
MeSH terms: Administration, Oral, Animals, Antioxidants, Ascorbic Acid, Physical Exertion, Hindlimb, Hypertrophy, Male, Muscle Contraction, Muscle Proteins, Organ Size, Phosphorylation, Time Factors, Signal Transduction, Rats, Wistar, Oxidative Stress, Muscle, Skeletal, Mitogen-Activated Protein Kinase 1, Ribosomal Protein S6 Kinases, 70-kDa, SKP Cullin F-Box Protein Ligases, Mitogen-Activated Protein Kinase 3, Rats

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Vitamin C Administration Attenuates Overload-Induced Skeletal Muscle Hypertrophy in Rats

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