Cortex-sparing fiber dissection: an improved method for the study of white matter anatomy in the human brain
Open accessAbstract
Classical fiber dissection of post mortem human brains enables us to isolate a fiber tract by removing the cortex and overlying white matter. In the current work, a modification of the dissection methodology is presented that preserves the cortex and the relationships within the brain during all stages of dissection, i.e. 'cortex-sparing fiber dissection'. Thirty post mortem human hemispheres (15 right side and 15 left side) were dissected using cortex-sparing fiber dissection. Magnetic resonance imaging study of a healthy brain was analyzed using diffusion tensor imaging (DTI)-based tractography software. DTI fiber tract reconstructions were compared with cortex-sparing fiber dissection results. The fibers of the superior longitudinal fasciculus (SLF), inferior fronto-occipital fasciculus (IFOF), inferior longitudinal fasciculus (ILF) and uncinate fasciculus (UF) were isolated so as to enable identification of their cortical terminations. Two segments of the SLF were identified: first, an indirect and superficial component composed of a horizontal and vertical segment; and second, a direct and deep component or arcuate fasciculus. The IFOF runs within the insula, temporal stem and sagittal stratum, and connects the frontal operculum with the occipital, parietal and temporo-basal cortex. The UF crosses the limen insulae and connects the orbito-frontal gyri with the anterior temporal lobe. Finally, a portion of the ILF was isolated connecting the fusiform gyrus with the occipital gyri. These results indicate that cortex-sparing fiber dissection facilitates study of the 3D anatomy of human brain tracts, enabling the tracing of fibers to their terminations in the cortex. Consequently, it is an important tool for neurosurgical training and neuroanatomical research.
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In vivo tractography of the human brain has been evaluated by subjective assessment of plausibility or qualitative visual agreement with post-mortem Klingler-like dissections.
“in vivo tractography of the human brain has been evaluated by subjective assessment of plausibility9, 10 or qualitative visual agreement with post-mortem Klingler-like dissections11, 12.”
From Tractography-based connectomes are dominated by false-positive connections (2016) · cites this paper as [11]
Post-mortem dissection with Klingler's method reveals the macroscopic organization of the human brain white matter, although this method shares some limitations of tractography in complex fiber configurations or near the cortex.
“Currently, post-mortem dissection with Klingler’s method reveals the macroscopic organization of the human brain white matter11, 62–64, although this method shares some of the mentioned limitations of tractography in complex fiber configurations or near the cortex.”
From Tractography-based connectomes are dominated by false-positive connections (2016) · cites this paper as [11]
The uncinate fasciculus connects the medial and lateral orbitofrontal cortex with anterior parts of the temporal lobe.
“the uncinate fasciculus that connects the medial and lateral orbitofrontal cortex with anterior parts of the temporal lobe”
From Damage to Broca’s area does not contribute to long-term speech production outcome after stroke (2021) · cites this paper as Martino et al., 2011
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- Authors
- Martino, J., De Witt Hamer, P. C., Vergani, F., Brogna, C., de Lucas, E. M., Vázquez‐Barquero, A., García‐Porrero, J. A., & Duffau, H.
- Affiliations
- Marqués de Valdecilla University Hospital (ES)
- Royal Victoria Infirmary (GB)
- Sapienza University of Rome (IT)
- Universidad de Cantabria (ES)
- Inserm (FR)
- Corresponding author
- Martino, Juan
- Venue
- Journal of Anatomy
- Publisher
- Wiley
- Published
- 2011-07-18
- Volume
- 219
- Issue
- 4
- Pages
- 531-541
- ISSN
- 0021-8782 (ISSN-L)
- 1469-7580
- PMC
- PMC3196758
- OpenAlex
- W2146172383
- Cited by
- 174
- References
- 47
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 14 · 2023: 9 · 2022: 12 · 2021: 14 · 2020: 13 · 2019: 10 · 2018: 7 · 2017: 13 · 2016: 14 · 2015: 14 · 2014: 12 · 2013: 14 · 2012: 12
- FWCI
- 9.30
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Anatomy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Newcastle University ePrints (Newcastle Univesity)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
- Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
- Physical Sciences › Engineering › Biomedical Engineering › Anatomy and Medical Technology
- Keywords
- white matter anatomy, superior longitudinal fasciculus, arcuate fasciculus, inferior fronto-occipital fasciculus, inferior longitudinal fasciculus, uncinate fasciculus, diffusion tensor tractography, neurosurgical training
- MeSH terms
- Aged, Aged, 80 and over, Brain, Dissection, Humans, Nerve Fibers, Neural Pathways
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