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Binding of a Cytosolic Protein to the Iron-Responsive Element of Human Ferritin Messenger RNA
Abstract
The human ferritin H chain messenger RNA contains a specific iron-responsive element (IRE) in its 5′ untranslated region, which mediates regulation by iron of ferritin translation. An RNA gel retardation assay was used to demonstrate the affinity of a specific cytosolic binding protein for the IRE. A single-base deletion in the IRE eliminated both the interaction of the cytoplasmic protein with the IRE and translational regulation. Thus, the regulatory potential of the IRE correlates with its capacity to specifically interact with proteins. Titration curves of binding activity after treatment of cells with an iron chelator suggest that the factor acts as a repressor of ferritin translation.
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“Cultured neurons from mice deficient in PERK (PKR-like ER kinase), which have an impaired UPR, exhibited enhanced sensitivity to 6-OHDA treatment (266).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [266]
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Metadata
- Authors
- Rouault, T. A., Hentze, M. W., Caughman, S. W., Harford, J. B., & Klausner, R. D.
- Affiliations
- National Institutes of Health (US)
- Venue
- Science
- Published
- 1988-09-02
- Volume
- 241
- Issue
- 4870
- Pages
- 1207-1210
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2023361302
- Cited by
- 351
- References
- 16
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 4 · 2023: 2 · 2022: 2 · 2021: 2 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 3 · 2014: 6 · 2013: 3 · 2012: 3
- FWCI
- 24.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- iron-responsive element, iron-responsive element-binding protein, translational regulation, 5' untranslated region, single-base deletion, iron chelators
- MeSH terms
- Base Composition, Binding, Competitive, Carrier Proteins, Cytosol, Deferoxamine, Ferritins, Globins, Humans, Iron, Liver, RNA, Messenger, Protein Biosynthesis, RNA-Binding Proteins, Nucleocytoplasmic Transport Proteins
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