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Cited by 592Gut microbiota and healthNutritional Studies and DietNutrition, Genetics, and Disease

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Mediterranean diet intervention in overweight and obese subjects lowers plasma cholesterol and causes changes in the gut microbiome and metabolome independently of energy intake

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Meslier, Victoria, Laiola, Manolo, Roager, Henrik Munch, De Filippis, Francesca, Roume, Hugo, Quinquis, Benoit, Giacco, Rosalba, Mennella, Ilario, Ferracane, Rosalia, Pons, Nicolas, Pasolli, Edoardo, Rivellese, Angela, Dragsted, Lars Ove, Vitaglione, Paola, Ehrlich, Stanislav Dusko, Ercolini, Danilo·2020·Gut
DOI 10.1136/gutjnl-2019-320438PMC PMC7306983
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Abstract

Objectives This study aimed to explore the effects of an isocaloric Mediterranean diet (MD) intervention on metabolic health, gut microbiome and systemic metabolome in subjects with lifestyle risk factors for metabolic disease. Design Eighty-two healthy overweight and obese subjects with a habitually low intake of fruit and vegetables and a sedentary lifestyle participated in a parallel 8-week randomised controlled trial. Forty-three participants consumed an MD tailored to their habitual energy intakes (MedD), and 39 maintained their regular diets (ConD). Dietary adherence, metabolic parameters, gut microbiome and systemic metabolome were monitored over the study period. Results Increased MD adherence in the MedD group successfully reprogrammed subjects’ intake of fibre and animal proteins. Compliance was confirmed by lowered levels of carnitine in plasma and urine. Significant reductions in plasma cholesterol (primary outcome) and faecal bile acids occurred in the MedD compared with the ConD group. Shotgun metagenomics showed gut microbiome changes that reflected individual MD adherence and increase in gene richness in participants who reduced systemic inflammation over the intervention. The MD intervention led to increased levels of the fibre-degrading Faecalibacterium prausnitzii and of genes for microbial carbohydrate degradation linked to butyrate metabolism. The dietary changes in the MedD group led to increased urinary urolithins, faecal bile acid degradation and insulin sensitivity that co-varied with specific microbial taxa. Conclusion Switching subjects to an MD while maintaining their energy intake reduced their blood cholesterol and caused multiple changes in their microbiome and metabolome that are relevant in future strategies for the improvement of metabolic health.

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Figure 1 Adherence to the Mediterranean diet (MD) and changes in dietary and metabolic variables. (A) Box plots showing MD index score for controls (ConD) or treated subjects (MedD) during the intervention, the significance was tested by applying the post hoc Friedman-Nemenyi test for pairwise test of multiple comparisons within each group. (B) Percentage changes in dietary and metabolic variables are represented as spider chart. Changes in levels of dietary components consumption including (C) dietary fibre, (D) vegetable proteins/animal proteins ratio, (E) saturated to polyunsaturated fats ratio. Reduction in serum and urinary markers such as (F) plasma carnitine, (G) urinary carnitine and (H) total cholesterol. The significance was tested by applying unpaired Wilcoxon rank-sum tests for variation at the specific time point compared with baseline in MedD versus ConD. Orange boxes refer to controls and green boxes to Mediterranean subjects, respectively. Baseline, 0 weeks; 4w, 4 weeks; 8w, 8 weeks of nutritional intervention (*p<0.05, **p<0.01 and ***p<0.001).
Figure 1 Adherence to the Mediterranean diet (MD) and changes in dietary and metabolic variables. (A) Box plots showing MD index score for controls (ConD) or treated subjects (MedD) during the intervention, the significance was tested by applying the post hoc Friedman-Nemenyi test for pairwise test of multiple comparisons within each group. (B) Percentage changes in dietary and metabolic variables are represented as spider chart. Changes in levels of dietary components consumption including (C) dietary fibre, (D) vegetable proteins/animal proteins ratio, (E) saturated to polyunsaturated fats ratio. Reduction in serum and urinary markers such as (F) plasma carnitine, (G) urinary carnitine and (H) total cholesterol. The significance was tested by applying unpaired Wilcoxon rank-sum tests for variation at the specific time point compared with baseline in MedD versus ConD. Orange boxes refer to controls and green boxes to Mediterranean subjects, respectively. Baseline, 0 weeks; 4w, 4 weeks; 8w, 8 weeks of nutritional intervention (*p<0.05, **p<0.01 and ***p<0.001).CC BY-NC · PubMed Central
Figure 2 Mediterranean diet changes the intestinal and systemic metabolome. Partial least squares discriminant analysis plots based on molecular features detected in (A) faeces and (B) urine. Subjects belonging to different categories were coloured according to diet and time points: MedD subjects at baseline (light green), after 4 weeks (green) and 8 weeks of intervention (dark green). ConD subjects at baseline (light orange), after 4 weeks (orange) and 8 weeks (dark orange) of intervention. The loading plots display vectors that contributed the most to variability of individual dataset; variables explaining the variance between the groups in (C) faecal and (D) urine metabolome are reported as bar plots.
Figure 2 Mediterranean diet changes the intestinal and systemic metabolome. Partial least squares discriminant analysis plots based on molecular features detected in (A) faeces and (B) urine. Subjects belonging to different categories were coloured according to diet and time points: MedD subjects at baseline (light green), after 4 weeks (green) and 8 weeks of intervention (dark green). ConD subjects at baseline (light orange), after 4 weeks (orange) and 8 weeks (dark orange) of intervention. The loading plots display vectors that contributed the most to variability of individual dataset; variables explaining the variance between the groups in (C) faecal and (D) urine metabolome are reported as bar plots.CC BY-NC · PubMed Central
Figure 3 Microbial diversity richness anticorrelates with inflammation. (A) Spearman’s correlation between variation of gut microbial gene richness and individual inflammatory status (serum hs-CRP) variation at the end of trial; n observation=62. (B) Violin plot showing differences in serum hs-CRP variation between subjects increasing (n=25, yellow) compared with subjects decreasing (n=37, light blue) gene richness at the end of trial. Statistical differences between groups were determined using unpaired Wilcoxon rank-sum tests. hs-CRP, high sensitivity C reactive protein.
Figure 3 Microbial diversity richness anticorrelates with inflammation. (A) Spearman’s correlation between variation of gut microbial gene richness and individual inflammatory status (serum hs-CRP) variation at the end of trial; n observation=62. (B) Violin plot showing differences in serum hs-CRP variation between subjects increasing (n=25, yellow) compared with subjects decreasing (n=37, light blue) gene richness at the end of trial. Statistical differences between groups were determined using unpaired Wilcoxon rank-sum tests. hs-CRP, high sensitivity C reactive protein.CC BY-NC · PubMed Central
Figure 4 Mediterranean diet (MD) affects gut microbiome composition. (A) Total delta MD index changes over the 4w-baseline period. Left: histograms of delta (4w-baseline) MD index (n=62). Right: linear regressions of microbiome similarity compared with delta 4w-baseline MD index. Microbiome similarity was estimated by Spearman correlations between microbial composition at 4 weeks and baseline within each individual. (B) Total MD index fractional changes (FCs) (4w-baseline)/baseline, used as proxy to measure the effort of adherence (n=62). Left: distribution of individuals relative to MD index FC. Right: linear regressions of microbiome similarity and FC. Spearman correlations (rho and p values) are reported, excluding outliers, for ConD (n=31) and MedD (n=26) groups, respectively. 4w, 4 weeks.
Figure 4 Mediterranean diet (MD) affects gut microbiome composition. (A) Total delta MD index changes over the 4w-baseline period. Left: histograms of delta (4w-baseline) MD index (n=62). Right: linear regressions of microbiome similarity compared with delta 4w-baseline MD index. Microbiome similarity was estimated by Spearman correlations between microbial composition at 4 weeks and baseline within each individual. (B) Total MD index fractional changes (FCs) (4w-baseline)/baseline, used as proxy to measure the effort of adherence (n=62). Left: distribution of individuals relative to MD index FC. Right: linear regressions of microbiome similarity and FC. Spearman correlations (rho and p values) are reported, excluding outliers, for ConD (n=31) and MedD (n=26) groups, respectively. 4w, 4 weeks.CC BY-NC · PubMed Central
Figure 5 Faecal BA concentrations over the nutritional intervention. Parallel coordinate plot showing variations of faecal (A) total, (B) primary and (C) secondary BA concentrations within the MedD group during the intervention. The red triangles indicate mean values, the lines connecting dots are used to indicate the same sample at each time point. The significance was tested by applying the post hoc Friedman-Nemenyi test for pairwise test of multiple comparisons within each group. (D) In the box plot the relative abundances of Bilophila wadsworthia are compared considering subjects falling in the highest quartile (n=16, green) and in the lowest quartile of reduction (n=16, blue) of secondary to primary BAs ratio after 4 weeks of treatment. Baseline, 0 weeks; 4w, 4 weeks; 8w, 8 weeks of nutritional intervention. H, highest quartile of reduction; L, lowest quartile of reduction; BAs, bile acids.
Figure 5 Faecal BA concentrations over the nutritional intervention. Parallel coordinate plot showing variations of faecal (A) total, (B) primary and (C) secondary BA concentrations within the MedD group during the intervention. The red triangles indicate mean values, the lines connecting dots are used to indicate the same sample at each time point. The significance was tested by applying the post hoc Friedman-Nemenyi test for pairwise test of multiple comparisons within each group. (D) In the box plot the relative abundances of Bilophila wadsworthia are compared considering subjects falling in the highest quartile (n=16, green) and in the lowest quartile of reduction (n=16, blue) of secondary to primary BAs ratio after 4 weeks of treatment. Baseline, 0 weeks; 4w, 4 weeks; 8w, 8 weeks of nutritional intervention. H, highest quartile of reduction; L, lowest quartile of reduction; BAs, bile acids.CC BY-NC · PubMed Central
Figure 6 MD intervention determines a reduction of faecal branched-chain fatty acid (BCFA) concentrations and higher levels of Faecalibacterium prausnitzii and Lachnospiraceae taxa. Parallel coordinate plot showing variations of (A) valerate, (B) isovalerate, (C) isobutyrate and (D) 2-methylbutyrate faecal concentrations within MedD population. The red triangles indicate mean values, the lines connecting dots are used to indicate the same sample at each time point. The significance was tested by applying the post hoc Friedman-Nemenyi test for pairwise test of multiple comparisons within each group. In the box plots, the relative abundances of (E) F. prausnitzii 3 and (F) Lachnospiraceae family are compared considering subjects falling in the highest quartile (n=16, violet) and in the lowest quartile (n=16, purple) of faecal butyrate increase after 4 weeks of treatment. Statistical differences between groups were determined using Wilcoxon rank-sum tests. Baseline, 0 weeks; 4w, 4 weeks; 8w, 8 weeks of intervention. H, highest quartile of increase; L, lowest quartile of increase.
Figure 6 MD intervention determines a reduction of faecal branched-chain fatty acid (BCFA) concentrations and higher levels of Faecalibacterium prausnitzii and Lachnospiraceae taxa. Parallel coordinate plot showing variations of (A) valerate, (B) isovalerate, (C) isobutyrate and (D) 2-methylbutyrate faecal concentrations within MedD population. The red triangles indicate mean values, the lines connecting dots are used to indicate the same sample at each time point. The significance was tested by applying the post hoc Friedman-Nemenyi test for pairwise test of multiple comparisons within each group. In the box plots, the relative abundances of (E) F. prausnitzii 3 and (F) Lachnospiraceae family are compared considering subjects falling in the highest quartile (n=16, violet) and in the lowest quartile (n=16, purple) of faecal butyrate increase after 4 weeks of treatment. Statistical differences between groups were determined using Wilcoxon rank-sum tests. Baseline, 0 weeks; 4w, 4 weeks; 8w, 8 weeks of intervention. H, highest quartile of increase; L, lowest quartile of increase.CC BY-NC · PubMed Central

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Authors
Meslier, V., Laiola, M., Roager, H. M., De Filippis, F., Roume, H., Quinquis, B., Giacco, R., Mennella, I., Ferracane, R., Pons, N., Pasolli, E., Rivellese, A., Dragsted, L. O., Vitaglione, P., Ehrlich, S. D., & Ercolini, D.
Affiliations
  • Université Paris-Saclay (FR)
  • University of Naples Federico II (IT)
  • University of Copenhagen (DK)
  • Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy
  • Université Paris-Saclay, INRAE, MGP, Jouy-en-Josas, France
  • Institute of Food Science (IT)
  • Federico II University Hospital (IT)
Corresponding author
Ercolini, Danilo
ORCID
Venue
Gut
Publisher
BMJ
Published
2020-02-19
Volume
69
Issue
7
Pages
1258-1268
ISSN
  • 0017-5749 (ISSN-L)
  • 1468-3288
DOI
10.1136/gutjnl-2019-320438
PMC
PMC7306983
OpenAlex
W3007138124
Cited by
583
References
69
Citations by year
2026: 67 · 2025: 116 · 2024: 118 · 2023: 87 · 2022: 85 · 2021: 73 · 2020: 36
FWCI
23.17
Type
journal article
Language
en
Open access
Licence
Locations
  • Gut (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by-nc)
  • HighWire Press Open Archive (submittedVersion)
  • INFM-OAR (INFN Catania) (submittedVersion, cc-by-nc)
Funders
  • Agence Nationale de la Recherche (ANR-11-DPBS-0001, ANR-15-HDIM-0002)
  • Agria Djurförsäkring
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Nutrition, Genetics, and Disease
Keywords
Mediterranean diet, overweight, obesity, serum cholesterol, gut microbiota, metabolome, isocaloric diet, dietary adherence, Faecalibacterium prausnitzii, butyrate metabolism, bile acids, urolithins, insulin sensitivity, systemic inflammation, dietary fiber, L-carnitine, metabolic health
MeSH terms
Gastrointestinal Microbiome, Adult, Energy Intake, Cholesterol, Female, Humans, Male, Obesity, Diet, Mediterranean, Overweight, Metabolome

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