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Cited by 80Muscle Physiology and DisordersMuscle metabolism and nutritionExercise and Physiological Responses

Influence of preexercise muscle glycogen content on transcriptional activity of metabolic and myogenic genes in well-trained humans

Churchley, Emmanuel G., Coffey, Vernon G., Pedersen, David J., Shield, Anthony, Carey, Kate A., Cameron-Smith, David, Hawley, John A.·2007·Journal of Applied Physiology
DOI 10.1152/japplphysiol.01260.2006
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Abstract

To determine whether preexercise muscle glycogen content influences the transcription of several early-response genes involved in the regulation of muscle growth, seven male strength-trained subjects performed one-legged cycling exercise to exhaustion to lower muscle glycogen levels (Low) in one leg compared with the leg with normal muscle glycogen (Norm) and then the following day completed a unilateral bout of resistance training (RT). Muscle biopsies from both legs were taken at rest, immediately after RT, and after 3 h of recovery. Resting glycogen content was higher in the control leg (Norm leg) than in the Low leg (435 +/- 87 vs. 193 +/- 29 mmol/kg dry wt; P < 0.01). RT decreased glycogen content in both legs (P < 0.05), but postexercise values remained significantly higher in the Norm than the Low leg (312 +/- 129 vs. 102 +/- 34 mmol/kg dry wt; P < 0.01). GLUT4 (3-fold; P < 0.01) and glycogenin mRNA abundance (2.5-fold; not significant) were elevated at rest in the Norm leg, but such differences were abolished after exercise. Preexercise mRNA abundance of atrogenes was also higher in the Norm compared with the Low leg [atrogin: approximately 14-fold, P < 0.01; RING (really interesting novel gene) finger: approximately 3-fold, P < 0.05] but decreased for atrogin in Norm following RT (P < 0.05). There were no differences in the mRNA abundance of myogenic regulatory factors and IGF-I in the Norm compared with the Low leg. Our results demonstrate that 1) low muscle glycogen content has variable effects on the basal transcription of select metabolic and myogenic genes at rest, and 2) any differences in basal transcription are completely abolished after a single bout of heavy resistance training. We conclude that commencing resistance exercise with low muscle glycogen does not enhance the activity of genes implicated in promoting hypertrophy.

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Authors
Churchley, E. G., Coffey, V. G., Pedersen, D. J., Shield, A., Carey, K. A., Cameron-Smith, D., & Hawley, J. A.
Affiliations
  • RMIT University (AU)
  • Deakin University (AU)
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
2007-04
Volume
102
Issue
4
Pages
1604-1611
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/japplphysiol.01260.2006
OpenAlex
W2034106326
Cited by
80
References
57
Citations by year
2026: 1 · 2025: 3 · 2023: 1 · 2022: 4 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 9 · 2014: 4 · 2013: 9 · 2012: 4
FWCI
3.04
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • RMIT Research Repository (RMIT University Library) (submittedVersion)
  • Figshare (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
muscle glycogen, resistance training, gene transcription, GLUT4, glycogenin, ring finger, myogenic regulatory factors, insulin-like growth factor I, muscle hypertrophy, muscle atrophy, exercise-induced gene expression
MeSH terms
Adult, Exercise Test, Glycogen, Humans, Male, Muscle Proteins, Physical Endurance, Physical Fitness, Rest, Transcriptional Activation, Myogenic Regulatory Factors, Muscle, Skeletal

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