Bioengineering human myocardium on native extracellular matrix
Open accessAbstract
Rationale: More than 25 million individuals have heart failure worldwide, with ≈4000 patients currently awaiting heart transplantation in the United States. Donor organ shortage and allograft rejection remain major limitations with only ≈2500 hearts transplanted each year. As a theoretical alternative to allotransplantation, patient-derived bioartificial myocardium could provide functional support and ultimately impact the treatment of heart failure. Objective: The objective of this study is to translate previous work to human scale and clinically relevant cells for the bioengineering of functional myocardial tissue based on the combination of human cardiac matrix and human induced pluripotent stem cell–derived cardiomyocytes. Methods and Results: To provide a clinically relevant tissue scaffold, we translated perfusion-decellularization to human scale and obtained biocompatible human acellular cardiac scaffolds with preserved extracellular matrix composition, architecture, and perfusable coronary vasculature. We then repopulated this native human cardiac matrix with cardiomyocytes derived from nontransgenic human induced pluripotent stem cells and generated tissues of increasing 3-dimensional complexity. We maintained such cardiac tissue constructs in culture for 120 days to demonstrate definitive sarcomeric structure, cell and matrix deformation, contractile force, and electrical conduction. To show that functional myocardial tissue of human scale can be built on this platform, we then partially recellularized human whole-heart scaffolds with human induced pluripotent stem cell–derived cardiomyocytes. Under biomimetic culture, the seeded constructs developed force-generating human myocardial tissue and showed electrical conductivity, left ventricular pressure development, and metabolic function. Conclusions: Native cardiac extracellular matrix scaffolds maintain matrix components and structure to support the seeding and engraftment of human induced pluripotent stem cell–derived cardiomyocytes and enable the bioengineering of functional human myocardial-like tissue of multiple complexities.
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Engineered tissues with contractile forces and conduction properties approaching native myocardium have been reported by several groups.
“A number of groups have reported engineered tissues with contractile forces and conduction properties approaching physiologic levels observed in native myocardium [8,13,31,32].”
From Bioengineering an electro-mechanically functional miniature ventricular heart chamber from human pluripotent stem cells (2018) · cites this paper as [31]
The Results (Recellularization of myocardial slices and fibers; page unavailable) report contractile force and a conduction velocity of 32 ± 56 cm/s in engineered tissue. But the reported slice force reached 33.8% of native murine embryonic heart tissue, slice strain was below normal adult myocardium, and the copy gives no native-myocardium benchmark for conduction velocity. It therefore supports the presence of mechanical and electrical function, but not the full claim that both properties approached physiologic levels. (LLM, conf=0.85)
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- Authors
- Guyette, J. P., Charest, J. M., Mills, R. W., Jank, B. J., Moser, P. T., Gilpin, S. E., Gershlak, J. R., Okamoto, T., Gonzalez, G., Milan, D. J., Gaudette, G. R., & Ott, H. C.
- Affiliations
- Worcester Polytechnic Institute (US)
- From the Center for Regenerative Medicine (J.P.G., J.M.C., B.J.J., P.T.M., S.E.G., T.O., G.G., H.C.O.), Cardiovascular Research Center (R.W.M., D.J.M.), Division of Cardiology (D.J.M.), and Division of Thoracic Surgery, Department of Surgery (H.C.O.), Massachusetts General Hospital, Boston, MA; Harvard Medical School, Boston, MA (J.P.G., B.J.J., P.T.M., S.E.G., G.G., H.C.O.); Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA (J.R.G., G.R.G.); and Harvard Stem Cell...
- ORCID
- Venue
- Circulation Research
- Published
- 2016-01-08
- Volume
- 118
- Issue
- 1
- Pages
- 56-72
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- PMC
- PMC4740234
- OpenAlex
- W1821166167
- Cited by
- 343
- References
- 99
- Citations by year
- 2026: 13 · 2025: 19 · 2024: 19 · 2023: 26 · 2022: 31 · 2021: 50 · 2020: 37 · 2019: 36 · 2018: 45 · 2017: 38 · 2016: 27 · 2015: 2
- FWCI
- 37.59
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (1dp2od008749-01)
- National Heart, Lung, and Blood Institute (R01HL115282, R21HL108663, R21 HL108663-2)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK057521, P30DK043351)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (P30AR066261)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Mechanical Circulatory Support Devices
- Keywords
- bioartificial heart, human myocardium, cardiac extracellular matrix, decellularization, perfusion decellularization, iPSC-derived cardiomyocytes, heart failure, heart transplantation, cardiac tissue engineering, recellularization, electrical conduction, contractile force, left ventricular pressure
- MeSH terms
- Adult, Aged, Cell Differentiation, Extracellular Matrix, Female, Humans, Male, Middle Aged, Myocardium, Pluripotent Stem Cells, Bioengineering
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