How mRNA therapeutics are entering the monoclonal antibody field
Open accessAbstract
In 1975, Milstein and Köhler revolutionized the medical world with the development of the hybridoma technique to produce monoclonal antibodies. Since then, monoclonal antibodies have entered almost every branch of biomedical research. Antibodies are now used as frontline therapeutics in highly divergent indications, ranging from autoimmune disease over allergic asthma to cancer. Wider accessibility and implementation of antibody-based therapeutics is however hindered by manufacturing challenges and high development costs inherent to protein-based drugs. For these reasons, alternative ways are being pursued to produce and deliver antibodies more cost-effectively without hampering safety. Over the past decade, messenger RNA (mRNA) based drugs have emerged as a highly appealing new class of biologics that can be used to encode any protein of interest directly in vivo. Whereas current clinical efforts to use mRNA as a drug are mainly situated at the level of prophylactic and therapeutic vaccination, three recent preclinical studies have addressed the feasibility of using mRNA to encode therapeutic antibodies directly in vivo. Here, we highlight the potential of mRNA-based approaches to solve several of the issues associated with antibodies produced and delivered in protein format. Nonetheless, we also identify key hurdles that mRNA-based approaches still need to take to fulfill this potential and ultimately replace the current protein antibody format.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Naked nucleic acids have a short half-life due to sensitivity to nuclease degradation.
“Due to their great sensitivity to nuclease degradation, naked nucleic acids have a short half-life.309”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [309]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Continuous cultures of fused cells secreting antibody of predefined specificity
Köhler, G., & Milstein, C. (1975) · Nature · doi:10.1038/256495a0 · Cited by 17,200 · see citing works
Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3
Alexopoulou, L., Holt, A. C., Medzhitov, R., & Flavell, R. A. (2001) · Nature · doi:10.1038/35099560 · Cited by 6,129 · see citing works
Direct Gene Transfer into Mouse Muscle in Vivo
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173 · see citing works
Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
Heil, F., Hemmi, H., Hochrein, H., Ampenberger, F., Kirschning, C., Akira, S., Lipford, G., Wagner, H., & Bauer, S. (2004) · Science · doi:10.1126/science.1093620 · Cited by 3,947 · see citing works
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses
Kato, H., Takeuchi, O., Sato, S., Yoneyama, M., Yamamoto, M., Matsui, K., Uematsu, S., Jung, A., Kawai, T., Ishii, K. J., et al. (2006) · Nature · doi:10.1038/nature04734 · Cited by 3,888 · see citing works
Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA
Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., & Reis e Sousa, C. (2004) · Science · doi:10.1126/science.1093616 · Cited by 3,444 · see citing works
Karikó, K., Buckstein, M., Ni, H., & Weissman, D. (2005) · Immunity · doi:10.1016/j.immuni.2005.06.008 · Cited by 2,728 · see citing works
Warren, L., Manos, P. D., Ahfeldt, T., Loh, Y.-H., Li, H., Lau, F., Ebina, W., Mandal, P. K., Smith, Z. D., Meissner, A., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.012 · Cited by 2,662 · see citing works
Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein
Ulmer, J. B., Donnelly, J., Parker, S. E., Rhodes, G. H., Felgner, P. L., Dwarki, V., Gromkowski, S. H., Deck, R. R., DeWitt, C. M., Friedman, A., et al. (1993) · Science · doi:10.1126/science.8456302 · Cited by 2,318 · see citing works
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates
Pichlmair, A., Schulz, O., Tan, C. P., Näslund, T. I., Liljeström, P., Weber, F., & Reis e Sousa, C. (2006) · Science · doi:10.1126/science.1132998 · Cited by 2,210 · see citing works
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
Milligan, J., Groebe, D., Witherell, G. W., & Uhlenbeck, O. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.21.8783 · Cited by 1,971 · see citing works
Karikó, K., Muramatsu, H., Welsh, F. A., Ludwig, J., Kato, H., Akira, S., & Weissman, D. (2008) · Molecular Therapy · doi:10.1038/mt.2008.200 · Cited by 1,930 · see citing works
Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy
Kranz, L. M., Diken, M., Haas, H., Kreiter, S., Loquai, C., Reuter, K. C., Meng, M., Fritz, D., Vascotto, F., Hefesha, H., et al. (2016) · Nature · doi:10.1038/nature18300 · Cited by 1,771 · see citing works
Therapeutic antibodies: successes, limitations and hopes for the future
Chames, P., Van Regenmortel, M., Weiss, E., & Baty, D. (2009) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2009.00190.x · Cited by 1,382 · see citing works
The therapeutic monoclonal antibody market
Ecker, D. M., Jones, S. D., & Levine, H. L. (2014) · mAbs · doi:10.4161/19420862.2015.989042 · Cited by 1,292 · see citing works
Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01
Zhou, T., Georgiev, I. S., Wu, X., Yang, Z., Dai, K., Finzi, A., Kwon, Y. D., Scheid, J. F., Shi, W., Xu, L., et al. (2010) · Science · doi:10.1126/science.1192819 · Cited by 1,132 · see citing works
Tumor Regression in Cancer Patients by Very Low Doses of a T Cell–Engaging Antibody
Bargou, R., Leo, E., Zugmaier, G., Klinger, M., Goebeler, M., Knop, S., Noppeney, R., Viardot, A., Hess, G., Schuler, M., et al. (2008) · Science · doi:10.1126/science.1158545 · Cited by 1,078 · see citing works
Pardi, N., Tuyishime, S., Muramatsu, H., Kariko, K., Mui, B. L., Tam, Y. K., Madden, T. D., Hope, M. J., & Weissman, D. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.007 · Cited by 1,014 · see citing works
Topp, M., Kufer, P., Gokbuget, N., Goebeler, M., Klinger, M., Neumann, S., Horst, H., Raff, T., Viardot, A., Schmid, M., et al. (2011) · Journal of Clinical Oncology · doi:10.1200/jco.2010.32.7270 · Cited by 914 · see citing works
Karikó, K., Muramatsu, H., Ludwig, J., & Weissman, D. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr695 · Cited by 898 · see citing works
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
Doyon, Y., McCammon, J. M., Miller, J. C., Faraji, F., Ngo, C., Katibah, G. E., Amora, R., Hocking, T. D., Zhang, L., Rebar, E. J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1409 · Cited by 896 · see citing works
The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency.
Gallie, D. R. (1991) · Genes & Development · doi:10.1101/gad.5.11.2108 · Cited by 868 · see citing works
The enzymes and control of eukaryotic mRNA turnover
Parker, R., & Song, H. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb724 · Cited by 813 · see citing works
Züst, R., Cervantes-Barragan, L., Habjan, M., Maier, R., Neuman, B. W., Ziebuhr, J., Szretter, K. J., Baker, S. C., Barchet, W., Diamond, M. S., et al. (2011) · Nature Immunology · doi:10.1038/ni.1979 · Cited by 811 · see citing works
Expression of therapeutic proteins after delivery of chemically modified mRNA in mice
Kormann, M. S. D., Hasenpusch, G., Aneja, M. K., Nica, G., Flemmer, A. W., Herber-Jonat, S., Huppmann, M., Mays, L. E., Illenyi, M., Schams, A., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1733 · Cited by 733 · see citing works
Clinical Pharmacokinetics of Therapeutic Monoclonal Antibodies
Keizer, R. J., Huitema, A. D. R., Schellens, J. H. M., & Beijnen, J. H. (2010) · Clinical Pharmacokinetics · doi:10.2165/11531280-000000000-00000 · Cited by 725 · see citing works
Andries, O., Mc Cafferty, S., De Smedt, S. C., Weiss, R., Sanders, N. N., & Kitada, T. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.051 · Cited by 701 · see citing works
Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation
Anderson, B. R., Muramatsu, H., Nallagatla, S. R., Bevilacqua, P. C., Sansing, L. H., Weissman, D., & Karikó, K. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq347 · Cited by 682 · see citing works
Engineering novel binding proteins from nonimmunoglobulin domains
Binz, H. K., Amstutz, P., & Plückthun, A. (2005) · Nature Biotechnology · doi:10.1038/nbt1127 · Cited by 676 · see citing works
Nonviral delivery of self-amplifying RNA vaccines
Geall, A. J., Verma, A., Otten, G. R., Shaw, C. A., Hekele, A., Banerjee, K., Cu, Y., Beard, C. W., Brito, L. A., Krucker, T., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1209367109 · Cited by 675 · see citing works
Bahl, K., Senn, J. J., Yuzhakov, O., Bulychev, A., Brito, L. A., Hassett, K. J., Laska, M. E., Smith, M., Almarsson, Ö., Thompson, J., et al. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2017.03.035 · Cited by 642 · see citing works
Holtkamp, S., Kreiter, S., Selmi, A., Simon, P., Koslowski, M., Huber, C., Türeci, O., & Sahin, U. (2006) · Blood · doi:10.1182/blood-2006-04-015024 · Cited by 625 · see citing works
Complement activation-related pseudoallergy: A new class of drug-induced acute immune toxicity
Szebeni, J. (2005) · Toxicology · doi:10.1016/j.tox.2005.07.023 · Cited by 610 · see citing works
Alternative molecular formats and therapeutic applications for bispecific antibodies
Spiess, C., Zhai, Q., & Carter, P. J. (2015) · Molecular Immunology · doi:10.1016/j.molimm.2015.01.003 · Cited by 607 · see citing works
Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy
Plückthun, A. (2011) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-010611-134654 · Cited by 578 · see citing works
Thess, A., Grund, S., Mui, B. L., Hope, M. J., Baumhof, P., Fotin-Mleczek, M., & Schlake, T. (2015) · Molecular Therapy · doi:10.1038/mt.2015.103 · Cited by 513 · see citing works
Mui, B. L., Tam, Y. K., Jayaraman, M., Ansell, S. M., Du, X., Tam, Y. Y. C., Lin, P. J. C., Chen, S., Narayanannair, J. K., Rajeev, K. G., et al. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.66 · Cited by 509 · see citing works
Van Tendeloo, V. F. I., Ponsaerts, P., Lardon, F., Nijs, G., Lenjou, M., Van Broeckhoven, C., Van Bockstaele, D. R., & Berneman, Z. N. (2001) · Blood · doi:10.1182/blood.v98.1.49 · Cited by 468 · see citing works
Anderson, B. R., Muramatsu, H., Jha, B. K., Silverman, R. H., Weissman, D., & Kariko, K. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr586 · Cited by 356 · see citing works
Pardi, N., Secreto, A. J., Shan, X., Debonera, F., Glover, J., Yi, Y., Muramatsu, H., Ni, H., Mui, B. L., Tam, Y. K., et al. (2017) · Nature Communications · doi:10.1038/ncomms14630 · Cited by 352 · see citing works
Polymer–Lipid Nanoparticles for Systemic Delivery of mRNA to the Lungs
Kaczmarek, J. C., Patel, A. K., Kauffman, K. J., Fenton, O. S., Webber, M. J., Heartlein, M. W., DeRosa, F., & Anderson, D. G. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201608450 · Cited by 337 · see citing works
Fotin-Mleczek, M., Duchardt, K. M., Lorenz, C., Pfeiffer, R., Ojkić-Zrna, S., Probst, J., & Kallen, K.-J. (2011) · Journal of Immunotherapy · doi:10.1097/cji.0b013e3181f7dbe8 · Cited by 335 · see citing works
Alternative non-antibody scaffolds for molecular recognition
Skerra, A. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.04.010 · Cited by 300 · see citing works
Elimination of large tumors in mice by mRNA-encoded bispecific antibodies
Stadler, C. R., Bähr-Mahmud, H., Celik, L., Hebich, B., Roth, A. S., Roth, R. P., Karikó, K., Türeci, Ö., & Sahin, U. (2017) · Nature Medicine · doi:10.1038/nm.4356 · Cited by 290 · see citing works
Fenton, O. S., Kauffman, K. J., Kaczmarek, J. C., McClellan, R. L., Jhunjhunwala, S., Tibbitt, M. W., Zeng, M. D., Appel, E. A., Dorkin, J. R., Mir, F. F., et al. (2017) · Advanced Materials · doi:10.1002/adma.201606944 · Cited by 289 · see citing works
Karikó, K., Muramatsu, H., Keller, J. M., & Weissman, D. (2012) · Molecular Therapy · doi:10.1038/mt.2012.7 · Cited by 282 · see citing works
Paunovska, K., Sago, C. D., Monaco, C. M., Hudson, W. H., Castro, M. G., Rudoltz, T. G., Kalathoor, S., Vanover, D. A., Santangelo, P. J., Ahmed, R., et al. (2018) · Nano Letters · doi:10.1021/acs.nanolett.8b00432 · Cited by 277 · see citing works
A mAb recognizing a surface antigen of <i>Mycobacterium tuberculosis</i> enhances host survival
Teitelbaum, R., Glatman-Freedman, A., Chen, B., Robbins, J. B., Unanue, E., Casadevall, A., & Bloom, B. R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15688 · Cited by 275 · see citing works
Vaccine Mediated Protection Against Zika Virus-Induced Congenital Disease
Richner, J. M., Jagger, B. W., Shan, C., Fontes, C. R., Dowd, K. A., Cao, B., Himansu, S., Caine, E. A., Nunes, B. T. D., Medeiros, D. B. A., et al. (2017) · Cell · doi:10.1016/j.cell.2017.06.040 · Cited by 267 · see citing works
Reichert, J. M. (2016) · mAbs · doi:10.1080/19420862.2016.1269580 · Cited by 244 · see citing works
RNA-Based Vaccines in Cancer Immunotherapy
McNamara, M. A., Nair, S. K., & Holl, E. K. (2015) · Journal of Immunology Research · doi:10.1155/2015/794528 · Cited by 223 · see citing works
Kumar, V., Qin, J., Jiang, Y., Duncan, R. G., Brigham, B., Fishman, S., Nair, J. K., Akinc, A., Barros, S. A., & Kasperkovitz, P. V. (2014) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2014.61 · Cited by 222 · see citing works
Ledwith, B. J., Manam, S., Troilo, P. J., Barnum, A. B., Pauley, C. J., Griffiths, T. G., Harper, L. B., Beare, C. M., Bagdon, W., & Nichols, W. W. (2000) · Intervirology · doi:10.1159/000053993 · Cited by 219 · see citing works
Immunization of non-human primates with DNA vaccines
Liu, M. (1997) · Vaccine · doi:10.1016/s0264-410x(96)00280-0 · Cited by 215 · see citing works
Foreign nucleic acids as the stimulus to make interferon
Isaacs, A., Cox, R. A., & Rotem, Z. (1963) · The Lancet · doi:10.1016/s0140-6736(63)92585-6 · Cited by 211 · see citing works
Kumar, P., Sweeney, T. R., Skabkin, M. A., Skabkina, O. V., Hellen, C. U. T., & Pestova, T. V. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1321 · Cited by 210 · see citing works
Preclinical Evaluation of TriMix and Antigen mRNA-Based Antitumor Therapy
Lint, S. V., Goyvaerts, C., Maenhout, S. K., Goethals, L., Disy, A., Benteyn, D., Pen, J. J., Bonehill, A., Heirman, C., Breckpot, K., et al. (2012) · Cancer Research · doi:10.1158/0008-5472.can-11-2957 · Cited by 210 · see citing works
Lipid-based mRNA vaccine delivery systems
Midoux, P., & Pichon, C. (2014) · Expert Review of Vaccines · doi:10.1586/14760584.2015.986104 · Cited by 209 · see citing works
Probst, J., Weide, B., Scheel, B., Pichler, B. J., Hoerr, I., Rammensee, H.-G., & Pascolo, S. (2007) · Gene Therapy · doi:10.1038/sj.gt.3302964 · Cited by 206 · see citing works
In Vitro Transcription of Long RNA Containing Modified Nucleosides
Pardi, N., Muramatsu, H., Weissman, D., & Karikó, K. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5_2 · Cited by 204 · see citing works
Ambegia, E., Ansell, S. M., Cullis, P. R., Heyes, J. A., Palmer, L., & MacLachlan, I. (2005) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2005.02.001 · Cited by 203 · see citing works
Non-immunoglobulin scaffolds: a focus on their targets
Škrlec, K., Štrukelj, B., & Berlec, A. (2015) · Trends in biotechnology · doi:10.1016/j.tibtech.2015.03.012 · Cited by 199 · see citing works
Identification of the gene for an Escherichia coli poly(A) polymerase.
Cao, G. J., & Sarkar, N. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.21.10380 · Cited by 193 · see citing works
Diken, M., Kreiter, S., Selmi, A., Britten, C. M., Huber, C. G., Türeci, Ö., & Şahin, U. (2011) · Gene Therapy · doi:10.1038/gt.2011.17 · Cited by 192 · see citing works
Manam, S., Ledwith, B. J., Barnum, A. B., Troilo, P. J., Pauley, C. J., Harper, L. B., Griffiths, T. G., Niu, Z., Denisova, L., Follmer, T. T., et al. (2000) · Intervirology · doi:10.1159/000053994 · Cited by 183 · see citing works
HPLC purification of in vitro transcribed long RNA
Weissman, D., Pardi, N., Muramatsu, H., & Karikó, K. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5_3 · Cited by 180 · see citing works
Antibodies to a cell surface histone-like protein protect against Histoplasma capsulatum
Nosanchuk, J. D., Steenbergen, J. N., Shi, L., Deepe, G. S., & Casadevall, A. (2003) · Journal of Clinical Investigation · doi:10.1172/jci19361 · Cited by 178 · see citing works
Hemodynamic Changes Induced by Liposomes and Liposome-Encapsulated Hemoglobin in Pigs
Szebeni, J., Fontana, J. L., Wassef, N. M., et al. (1999) · Circulation · doi:10.1161/01.cir.99.17.2302 · Cited by 176 · see citing works
mRNA mediates passive vaccination against infectious agents, toxins, and tumors
Thran, M., Mukherjee, J., Pönisch, M., Fiedler, K., Thess, A., Mui, B. L., Hope, M. J., Tam, Y. K., Horscroft, N., Heidenreich, R., et al. (2017) · EMBO Molecular Medicine · doi:10.15252/emmm.201707678 · Cited by 166 · see citing works
MicroRNAs Enable mRNA Therapeutics to Selectively Program Cancer Cells to Self-Destruct
Jain, R., Frederick, J. P., Huang, E. Y., Burke, K. E., Mauger, D. M., Andrianova, E. A., Farlow, S. J., Siddiqui, S., Pimentel, J., Cheung-Ong, K., et al. (2018) · Nucleic Acid Therapeutics · doi:10.1089/nat.2018.0734 · Cited by 164 · see citing works
Venkatesan, S., Gershowitz, A., & Moss, B. A. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86118-5 · Cited by 161 · see citing works
Lorenz, C., Fotin‐Mleczek, M., Roth, G. D., Becker, C., Dam, T. C., Verdurmen, W. P. R., Brock, R. E., Probst, J., & Schlake, T. (2011) · RNA Biology · doi:10.4161/rna.8.4.15394 · Cited by 152 · see citing works
Pascolo, S. (2004) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.4.8.1285 · Cited by 152 · see citing works
Non-immunoglobulin based protein scaffolds
Löfblom, J., Frejd, F. Y., & Ståhl, S. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.06.002 · Cited by 147 · see citing works
Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection
Tiwari, P. M., Vanover, D. A., Lindsay, K. E., Bawage, S. S., Kirschman, J. L., Bhosle, S. M., Lifland, A. W., Zurla, C., & Santangelo, P. J. (2018) · Nature Communications · doi:10.1038/s41467-018-06508-3 · Cited by 139 · see citing works
Hovanessian, A. G. (2007) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2007.06.003 · Cited by 131 · see citing works
Vaccination with Messenger RNA (mRNA)
Pascolo, S. (2008) · Handbook of Experimental Pharmacology · doi:10.1007/978-3-540-72167-3_11 · Cited by 126 · see citing works
Mock, U., Machowicz, R., Hauber, I., Horn, S., Abramowski, P., Berdien, B., Hauber, J., & Fehse, B. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv469 · Cited by 126 · see citing works
De Beuckelaer, A., Pollard, C., Van Lint, S., Roose, K., Van Hoecke, L., Naessens, T., Udhayakumar, V. K., Smet, M., Sanders, N., Lienenklaus, S., et al. (2016) · Molecular Therapy · doi:10.1038/mt.2016.161 · Cited by 122 · see citing works
Self-citationBroos, K., Van der Jeught, K., Puttemans, J., Goyvaerts, C., Heirman, C., Dewitte, H., Verbeke, R., Lentacker, I., Thielemans, K., & Breckpot, K. (2016) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2016.38 · Cited by 121 · see citing works
Bispecific antibodies rise again
Garber, K. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4478 · Cited by 119 · see citing works
mRNA vaccine CV9103 and CV9104 for the treatment of prostate cancer
Rausch, S., Schwentner, C., Stenzl, A., & Bedke, J. (2014) · Human Vaccines & Immunotherapeutics · doi:10.4161/hv.29553 · Cited by 115 · see citing works
Beane, J. D., Lee, G., Zheng, Z., Mendel, M. C., Abate‐Daga, D., Bharathan, M., Black, M. A., Gandhi, N. H., Yu, Z., Chandran, S. S., et al. (2015) · Molecular Therapy · doi:10.1038/mt.2015.71 · Cited by 111 · see citing works
Type I Interferons Modulate CD8 + T Cell Immunity to mRNA Vaccines
Beuckelaer, A. D., Grooten, J., & Koker, S. D. (2017) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2017.01.006 · Cited by 109 · see citing works
Therapeutic anti‐tumor immunity triggered by injections of immunostimulating single‐stranded RNA
Scheel, B., Aulwurm, S., Probst, J., Stitz, L., Hoerr, I., Rammensee, H., Weller, M., & Pascolo, S. (2006) · European Journal of Immunology · doi:10.1002/eji.200635910 · Cited by 108 · see citing works
Monoclonal antibodies for prophylactic and therapeutic use against viral infections
Both, L., Banyard, A. C., van Dolleweerd, C., Wright, E., Ma, J. K.-C., & Fooks, A. R. (2013) · Vaccine · doi:10.1016/j.vaccine.2013.01.025 · Cited by 107 · see citing works
Biodegradable Amino-Ester Nanomaterials for Cas9 mRNA Delivery in Vitro and in Vivo
Zhang, X., Li, B., Luo, X., Zhao, W., Jiang, J., Zhang, C., Gao, M., Chen, X., & Dong, Y. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b08163 · Cited by 107 · see citing works
Historical Development of Monoclonal Antibody Therapeutics
Nissim, A., & Chernajovsky, Y. (2007) · Handbook of experimental pharmacology · doi:10.1007/978-3-540-73259-4_1 · Cited by 105 · see citing works
Direct gene transfer into muscle
Dankó, I., & Wolff, J. A. (1994) · Vaccine · doi:10.1016/0264-410x(94)90072-8 · Cited by 102 · see citing works
Activation of RIG-I-like receptor signal transduction
Bruns, A. M., & Horvath, C. M. (2011) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2011.630974 · Cited by 95 · see citing works
Muthumani, K., Block, P. D., Flingai, S., Muruganandam, N., Chaaithanya, I. K., Tingey, C., Wise, M. C., Reuschel, E. L., Chung, C. W., Muthumani, A., et al. (2016) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiw111 · Cited by 91 · see citing works
Lindborg, M., Cortez, E., Höidén‐Guthenberg, I., Gunneriusson, E., von Hage, E., Syud, F. A., Morrison, M., Abrahmsén, L., Herne, N., Pietras, K., et al. (2011) · Journal of Molecular Biology · doi:10.1016/j.jmb.2011.01.033 · Cited by 86 · see citing works
mRNA as a Versatile Tool for Exogenous Protein Expression
Kuhn, A. N., Beißert, T., Simon, P., Vallazza, B., Buck, J., Davies, B. P., Türeci, Ö., & Şahin, U. (2012) · Current Gene Therapy · doi:10.2174/156652312802762536 · Cited by 73 · see citing works
Emanuel, S. L., Engle, L. J., Chao, G., Zhu, R.-R., Cao, C., Lin, Z., Yamniuk, A. P., Hosbach, J., Brown, J. M., Fitzpatrick, E., et al. (2011) · mAbs · doi:10.4161/mabs.3.1.14168 · Cited by 72 · see citing works
Protection against dengue disease by synthetic nucleic acid antibody prophylaxis/immunotherapy
Flingai, S., Plummer, E. M., Patel, A. R., et al. (2015) · Scientific Reports · doi:10.1038/srep12616 · Cited by 71 · see citing works
Preventing nondesired RNA‐primed RNA extension catalyzed by T7 RNA polymerase
Nacheva, G. A., & Berzal‐Herranz, A. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03510.x · Cited by 62 · see citing works
Genetic delivery of an anti-RSV antibody to protect against pulmonary infection with RSV
Skaricic, D., Traube, C., De, B., Joh, J., Boyer, J., Crystal, R. G., & Worgall, S. (2008) · Virology · doi:10.1016/j.virol.2008.04.016 · Cited by 54 · see citing works
Muromonab-CD3 (Orthoclone OKT3): the first monoclonal antibody approved for therapeutic use.
Emmons, C., & Hunsicker, L. G. (1987) · PubMed · Cited by 52 · see citing works
Messenger <scp>RNA</scp>‐based vaccines: progress, challenges, applications
Kramps, T., & Probst, J. (2013) · WIREs RNA · doi:10.1002/wrna.1189 · Cited by 50 · see citing works
Benteyn, D., Anguille, S., Lint, S. V., Heirman, C., Nuffel, A. M. T. V., Corthals, J., Ochsenreither, S., Waelput, W., Beneden, K. V., Breckpot, K., et al. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.54 · Cited by 44 · see citing works
Blinatumomab for Acute Lymphoblastic Leukemia
Kantarjian, H. M., Jabbour, E., & Topp, M. S. (2017) · New England Journal of Medicine · doi:10.1056/nejmc1704012 · Cited by 31 · see citing works
Li, C., Qi, R., Singleterry, R., Hyle, J. W., Balch, A., Li, X., Sublett, J. E., Berns, H., Valentine, M. B., Valentine, V. A., et al. (2014) · Molecular and Cellular Biology · doi:10.1128/mcb.00023-14 · Cited by 29 · see citing works
Nakajima, K., & Yaoita, Y. (2015) · Biology Open · doi:10.1242/bio.201410009 · Cited by 23 · see citing works
Immunological effects of a novel RNA-based adjuvant in liver cancer patients
Circelli, L., Petrizzo, A., Tagliamonte, M., Heidenreich, R., Tornesello, M. L., Buonaguro, F. M., & Buonaguro, L. (2016) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-016-1923-5 · Cited by 21 · see citing works
The authors and a colleague reply
Kantarjian, H., Jabbour, E. J., & Topp, M. S. (2017) · New England Journal of Medicine · Cited by 10 · see citing works
Ejeskär, K., Fransson, S. M., Zaibak, F., et al. (2006) · International Journal of Molecular Medicine · doi:10.3892/ijmm.17.6.1011 · Cited by 8 · see citing works
Hoerr, I. (2000) · doi:10.1002/1521-4141(200001)30:1
Flagged first, then by citations
Cited by
Sorted by citations
The evolution of commercial drug delivery technologies
Vargason, A. M., Anselmo, A. C., & Mitragotri, S. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00698-w · Cited by 1,361 · see citing works
Smart nanoparticles for cancer therapy
Sun, L., Liu, H., Ye, Y., Lei, Y., Islam, R., Tan, S., Tong, R., Miao, Y.-B., & Cai, L. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01642-x · Cited by 1,073 · see citing works
Opportunities and Challenges in the Delivery of mRNA-Based Vaccines
Wadhwa, A., Aljabbari, A., Lokras, A., Foged, C., & Thakur, A. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12020102 · Cited by 584 · see citing works
Nanomaterial Delivery Systems for mRNA Vaccines
Buschmann, M. D., Carrasco, M. J., Alishetty, S., Paige, M., Alameh, M. G., & Weissman, D. (2021) · Vaccines · doi:10.3390/vaccines9010065 · Cited by 582 · see citing works
Three decades of messenger RNA vaccine development
Verbeke, R., Lentacker, I., De Smedt, S. C., & Dewitte, H. (2019) · Nano Today · doi:10.1016/j.nantod.2019.100766 · Cited by 342 · see citing works
mRNA Delivery for Therapeutic Anti-HER2 Antibody Expression In Vivo
Rybakova, Y., Kowalski, P. S., Huang, Y., Gonzalez, J. T., Heartlein, M. W., DeRosa, F., Delcassian, D., & Anderson, D. G. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.05.012 · Cited by 218 · see citing works
Erasmus, J. H., Archer, J., Fuerte-Stone, J., Khandhar, A. P., Voigt, E., Granger, B., Bombardi, R. G., Govero, J., Tan, Q., Durnell, L. A., et al. (2020) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2020.06.011 · Cited by 118 · see citing works
Pandey, M., Ojha, D., Bansal, S., Rode, A. B., & Chawla, G. (2021) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2021.101003 · Cited by 23 · see citing works
- Authors
- Van Hoecke, L., & Roose, K.
- Affiliations
- Ghent University (BE)
- Venue
- Journal of Translational Medicine
- Published
- 2019-02-22
- Volume
- 17
- Issue
- 1
- Pages
- 54-54
- ISSN
- 1479-5876 (ISSN-L)
- PMC
- PMC6387507
- OpenAlex
- W2947249571
- Cited by
- 205
- References
- 107
- Citations by year
- 2026: 22 · 2025: 27 · 2024: 42 · 2023: 33 · 2022: 32 · 2021: 33 · 2020: 14 · 2019: 2
- FWCI
- 1.79
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Translational Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Vlaams Instituut voor Biotechnologie
- Vlaamse regering
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- mRNA therapeutics, therapeutic antibodies, hybridoma, monoclonal antibodies, protein-based drugs, drug development costs, prophylactic and therapeutic vaccination
- MeSH terms
- Animals, Antibodies, Monoclonal, Humans, Models, Biological, RNA, Messenger, Gene Transfer Techniques, Bioreactors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review