Testosterone stimulates intracellular calcium release and mitogen-activated protein kinases via a G protein-coupled receptor in skeletal muscle cells
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Involvement of intracellular Ca(2+) and ERK1/2 phosphorylation in the fast nongenomic effects of androgens in myotubes was investigated. Testosterone or nandrolone produced fast (<1 min) and transient increases in intracellular Ca(2+) with an oscillatory pattern. Calcium signals were slightly reduced in Ca(2+)-free medium, but lack of oscillations was evident. Signals were blocked by U-73122 and xestospongin B, inhibitors of inositol 1,4,5-trisphosphate (IP(3)) pathway. Furthermore, IP(3) increased transiently 2- to 3-fold 45 sec after hormone addition. Cyproterone neither affected the fast Ca(2+) signal nor the increase in IP(3). Calcium increases could also be induced by the impermeant testosterone conjugated to BSA, and the effect of testosterone was abolished in cells incubated with guanosine 5'-O-(2-thiodiphosphate) or pertussis toxin. Stimulation of myotubes with testosterone, nandrolone, or testosterone conjugated to BSA increased immunodetectable phosphorylation of ERK1/2 within 5 min, and this effect was not inhibited by cyproterone. Phosphorylation was blocked by the use of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethylester, U-73122, and xestospongin B as well as by dominant negative Ras, MAPK kinase (MEK), or the MEK inhibitor PD-98059. In addition, guanosine 5'-O-(2-thiodiphosphate) or pertussis toxin blocked ERK1/2 phosphorylation. These results are consistent with a fast effect of testosterone, involving a G protein-linked receptor at the plasma membrane, IP(3)-mediated Ca(2+) signal, and the Ras/MEK/ERK pathway in muscle cells.
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Mammalian steroid hormones increase Ca2+ influx in a variety of cell types, including cardiac myocytes and skeletal muscle.
“Mammalian steroid hormones increase Ca2+ influx in a variety of cell types, including cardiac myocytes and skeletal muscle182,183.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [182]
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“Furthermore, ERK and Src are calcium-dependent kinase cascades suggesting AR could directly regulate them via mobilization of intracellular Ca levels (32,34).”
BackgroundHatanaka, Y., Hojo, Y., Mukai, H., Murakami, G., Komatsuzaki, Y., Kim, J., Ikeda, M., Hiragushi, A., Kimoto, T., & Kawato, S. (2014) · Brain Research · doi:10.1016/j.brainres.2014.12.011 · Cited by 98 · see citing works
“For peripheral cells, there are several other studies, reporting the rapid action of androgen through non-AR (Estrada et al., 2003; Papakonstanti et al., 2003).”
BackgroundGorelick-Feldman, J., Cohick, W., & Raskin, I. (2010) · Steroids · doi:10.1016/j.steroids.2010.03.008 · Cited by 96 · see citing works
“Mammalian steroid hormones increased Ca2+ influx in a variety of cell types including cardiac myocytes [6] and skeletal muscle [7].”
BackgroundPomara, C., Neri, M., Bello, S., Fiore, C., Riezzo, I., & Turillazzi, E. (2014) · Current Neuropharmacology · doi:10.2174/1570159x13666141210221434 · Cited by 98 · see citing works
“In addition, androgens can activate calcium dependent kinase pathways, such as extracellular signal-regulated kinase (ERK) or Src, which could phosphorylate the ARs and enhance its activity [113, 114].”
BackgroundKamanga‐Sollo, E., Pampusch, M. S., Xi, G., White, M. E., Hathaway, M. R., & Dayton, W. R. (2004) · Journal of Cellular Physiology · doi:10.1002/jcp.20000 · Cited by 92 · see citing works
“Recently, Estrada et al. (2003) have reported that testosterone stimulates intracellular calcium release and mitogen-activated protein kinases via a putative G-protein coupled, cell surface receptor in rat skeletal muscle cells.”
BackgroundMale sex hormones, aging, and inflammation
Ketchem, J. M., Bowman, E. J., & Isales, C. M. (2023) · Biogerontology · doi:10.1007/s10522-022-10002-1 · Cited by 90 · see citing works
Cook, C. J., & Crewther, B. T. (2012) · Hormones and Behavior · doi:10.1016/j.yhbeh.2011.09.006 · Cited by 86 · see citing works
Pergola, C., Rogge, A., Dodt, G., Northoff, H., Weinigel, C., Barz, D., Rådmark, O., Sautebin, L., & Werz, O. (2011) · The FASEB Journal · doi:10.1096/fj.11-182758 · Cited by 87 · see citing works
20-Hydroxyecdysone Increases Fiber Size in a Muscle-Specific Fashion in Rat
Tóth, N., Szabó, A., Kacsala, P., Héger, J., & Zádor, E. (2008) · Phytomedicine · doi:10.1016/j.phymed.2008.04.015 · Cited by 85 · see citing works
“The fast anabolic effect of steroids in skeletal muscle is exerted through signal transduction pathways and not via intracellular steroid receptors (Estrada et al. 2003).”
BackgroundBecerra-Díaz, M., Song, M., & Heller, N. M. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.01698 · Cited by 84 · see citing works
“Importantly, it has been reported that the androgen–AR complex can also mediate nongenomic changes (179) by causing calcium flux and by activating second messenger pathways including ERK, AKT, and MAPK, at least in cell lines (179–181).”
BackgroundHuman androgen deficiency: insights gained from androgen receptor knockout mouse models
Zajac, J. D., Rana, K., & Davey, R. A. (2014) · Asian Journal of Andrology · doi:10.4103/1008-682x.122590 · Cited by 84 · see citing works
Ca2+ oscillations induced by testosterone enhance neurite outgrowth
Estrada, M., Uhlén, P., & Ehrlich, B. E. (2006) · Journal of Cell Science · doi:10.1242/jcs.02775 · Cited by 81 · see citing works
“The presence of membrane binding sites for androgens has been previously suggested (Estrada et al., 2003; Lieberherr and Grosse, 1994), even in macrophages, which lack a classical iAR (Benten et al.”
BackgroundMethodInfluentialSarcopenia and Androgens: A Link between Pathology and Treatment
Basualto-Alarcón, C., Varela, D., Duran, J., Maass, R., & Estrada, M. (2014) · Frontiers in Endocrinology · doi:10.3389/fendo.2014.00217 · Cited by 80 · see citing works
“In skeletal muscle, we observed that testosterone stimulation induces rapid (<1 min) oscillation in intracellular Ca2+ signals, which begin as Ca2+ transients initiated in the cytosol before being propagated as waves of Ca2+ in the cytoplasm and nucleus (68, 70, 71).”
BackgroundEndocrinological Roles for Testosterone in Resistance Exercise Responses and Adaptations
Hooper, D. R., Kraemer, W. J., Focht, B. C., Volek, J. S., DuPont, W. H., Caldwell, L. K., & Maresh, C. M. (2017) · Sports Medicine · doi:10.1007/s40279-017-0698-y · Cited by 80 · see citing works
Cook, C. J., Kilduff, L. P., & Crewther, B. T. (2017) · Scandinavian Journal of Medicine and Science in Sports · doi:10.1111/sms.13041 · Cited by 79 · see citing works
Narayanan, R., Coss, C. C., Yepuru, M., Kearbey, J. D., Miller, D. D., & Dalton, J. T. (2008) · Molecular Endocrinology · doi:10.1210/me.2008-0160 · Cited by 77 · see citing works
Cárdenas, C., Liberona, J. L., Molgó, J., Colasante, C., Mignery, G. A., & Jaimovich, E. (2005) · Journal of Cell Science · doi:10.1242/jcs.02446 · Cited by 76 · see citing works
“Slow Ca2+ signals with similar characteristics, although displaying particular kinetics can also be elicited by androgens like testosterone (Estrada et al., 2000; Estrada et al., 2003), or hormones such as insulin-like growth factor (Espinosa et al., 2004).”
BackgroundBulldan, A., Dietze, R., Shihan, M., & Scheiner‐Bobis, G. (2016) · Cellular Signalling · doi:10.1016/j.cellsig.2016.04.015 · Cited by 78 · see citing works
Chen, R., Zeng, X., Zhang, R., Huang, J., Kuang, X., Yang, J., Liu, J., Tawfik, O. W., Thrasher, J. B., & Li, B. (2013) · Urologic Oncology Seminars and Original Investigations · doi:10.1016/j.urolonc.2013.05.011 · Cited by 75 · see citing works
Martín, A., Priego, T., & López-Calderón, A. (2018) · Advances in Experimental Medicine and Biology · doi:10.1007/978-981-13-1435-3_9 · Cited by 68 · see citing works
Holmes, S., Singh, M., Su, C., & Cunningham, R. L. (2016) · Endocrinology · doi:10.1210/en.2015-1738 · Cited by 73 · see citing works
An Inconvenient Variable: Sex Hormones and Their Impact on T Cell Responses
Brown, M. A., & Su, M. A. (2019) · The Journal of Immunology · doi:10.4049/jimmunol.1801403 · Cited by 70 · see citing works
“its effects through cytoplasmic signaling (13), and activation of nontranscriptional signaling mechanisms have also been described for PR and AR (14, 15).”
BackgroundDu, X., Lu, F. Y., & Kiriazis, H. (2006) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2005.10.016 · Cited by 68 · see citing works
Sex Steroid Receptors in Immune Cells
Kovats, S., Carreras, E., & Agrawal, H. (2009) · Springer eBooks · doi:10.1007/978-3-642-02155-8_3 · Cited by 67 · see citing works
“As is the case for ERs and PRs, the nongenomic or nonclassical pathway for androgen action has been documented in the reproductive, cardiovascular, immune, and musculoskeletal systems (Benten et al. 1997; Benten et al. 1999a; Guo et al. 2002; Estrada et al. 2003; Walker and Cheng 2005; Vicencio et al. 2006).”
BackgroundYolk androgens as pleiotropic mediators of physiological processes: A mechanistic review
Navara, K. J., & Mendonça, M. T. (2008) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2008.05.002 · Cited by 66 · see citing works
“, 1996) and also stimulates intracellular calcium release and mitogenactivated protein kinases in skeletal muscle cells (Estrada et al., 2003), contributing to the strength ofmuscle contraction.”
BackgroundThe role of sex steroid hormones in the pathophysiology and treatment of sarcopenia
Kim, Y. J., Tamadon, A., Park, H. T., Kim, H., & Ku, S. (2016) · Osteoporosis and Sarcopenia · doi:10.1016/j.afos.2016.06.002 · Cited by 67 · see citing works
“It is also shown that testosterone could activate ERK1/2 pathway in skeletal muscle [135].”
BackgroundInfluentialMcCullough, D., Webb, R., Enright, K. J., Lane, K. E., McVeigh, J., Stewart, C. E., & Davies, I. G. (2021) · Reviews in Endocrine and Metabolic Disorders · doi:10.1007/s11154-020-09616-y · Cited by 62 · see citing works
“These signalling cascades upregulate transcription, satellite cell proliferation, muscle protein synthesis and reduce apoptosis ultimately resulting in skeletal muscle hypertrophy [17, 54, 55, 60] (Fig.”
BackgroundHamdi, M. M., & Mutungi, G. (2009) · The Journal of Physiology · doi:10.1113/jphysiol.2009.182162 · Cited by 62 · see citing works
“These actions occur within seconds to minutes after administration of the hormone and are generally insensitive to inhibitors of the androgen receptor (Estrada et al. 2003), suggesting that they are regulated by cellular signalling pathways involving surface membrane receptors and second messengers (Heinlein & Chang,…”
BackgroundInfluentialNguyen, T. V., Yao, M., & Pike, C. J. (2007) · Endocrinology · doi:10.1210/en.2006-1469 · Cited by 62 · see citing works
Crewther, B. T., Cook, C. J., Gaviglio, C. M., Kilduff, L. P., & Drawer, S. (2011) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e3182185158 · Cited by 61 · see citing works
“Testosterone can also affect behavior (1,28) and influence (positively) calcium handling and muscle contractility (18,20).”
BackgroundTestosterone increases GLUT4‐dependent glucose uptake in cardiomyocytes
Wilson, C., Contreras‐Ferrat, A. E., Venegas, N., Osorio‐Fuentealba, C., Pávez, M., Montoya, K., Durán, J., Maass, R., Lavandero, S., & Estrada, M. (2013) · Journal of Cellular Physiology · doi:10.1002/jcp.24413 · Cited by 60 · see citing works
“An alternative mechanism could be mediated by a putative plasma membrane receptor (Estrada et al., 2003; Vicencio et al., 2006), similar to the one that is seen for G-proteincoupled receptor 30 (GPR30) for estrogens (Martensson et al., 2009; Prossnitz and Barton, 2011).”
BackgroundThe Effects of Androgens on T Cells: Clues to Female Predominance in Autoimmune Liver Diseases?
Henze, L., Schwinge, D., & Schramm, C. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.01567 · Cited by 58 · see citing works
“In addition to the classical cytoplasmic androgen receptor (AR), androgens can also bind and activate membrane androgen receptors (mAR) (21).”
BackgroundTestosterone-induced hypertrophy of L6 myoblasts is dependent upon Erk and mTOR
Wu, Y., Bauman, W. A., Blitzer, R. D., & Cardozo, C. P. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.08.127 · Cited by 56 · see citing works
Fu, R., Liu, J., Fan, J., Li, R., Li, D., Yin, J., & Cui, S. (2011) · Journal of Cellular Physiology · doi:10.1002/jcp.22710 · Cited by 56 · see citing works
“It has been reported that T induces a rapid change in Ca2þ level, which involves a G protein-coupled receptor at the plasma membrane in skeletal muscle (Estrada et al., 2003).”
BackgroundResultInfluentialVicencio, J. M., Estrada, M., Galvis, D. M. S., Bravo, R., Contreras‐Ferrat, A. E., Rotter, D., Szabadkai, G., Hill, J. A., Rothermel, B. A., Jaimovich, E., et al. (2011) · Mini-Reviews in Medicinal Chemistry · doi:10.2174/138955711795445880 · Cited by 52 · see citing works
“Nongenomic testosterone effects have been observed in skeletal muscle cells, via the release of Ca2+ from internal stores [44, 45].”
BackgroundAndrogen actions on endothelium functions and cardiovascular diseases.
Cai, J., Wen, J., Jiang, W., Lin, J., Yuan, H., & Zhu, Y. (2016) · PubMed Central · doi:10.11909/j.issn.1671-5411.2016.02.003 · Cited by 54 · see citing works
Exercise-Induced Hormone Elevations Are Related to Muscle Growth
Mangine, G. T., Hoffman, J. R., Gonzalez, A. M., Townsend, J. R., Wells, A. J., Jajtner, A. R., Beyer, K. S., Boone, C. H., Wang, R., Miramonti, A. A., et al. (2016) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0000000000001491 · Cited by 50 · see citing works
“, independent of its receptor) role where testosterone stimulates transient increases in intracellular calcium (10), which may temporarily elevate maximal force production (16).”
BackgroundRISING STARS: androgens and immune cell function
Ainslie, R. J., Simitsidellis, I., Kirkwood, P. M., & Gibson, D. A. (2024) · Journal of Endocrinology · doi:10.1530/joe-23-0398 · Cited by 48 · see citing works
“Binding of androgens to this receptor leads to activation of intracellular signalling pathways that result in the transcription of target genes (Kousteni et al. 2001, Estrada et al. 2003, Kang et al. 2004, Davey & Grossmann 2016, Thomas 2019).”
BackgroundShihan, M., Bulldan, A., & Scheiner‐Bobis, G. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2014.03.002 · Cited by 48 · see citing works
“While some investigators favor the exclusive participation of thewell-characterized cytosolic/nuclear androgen receptor (AR) in both pathways [9], others propose a membrane-bound AR, possibly from the family of G-protein-coupled receptors (GPCR), as mediator of several testosterone-induced effects [10–14].”
BackgroundDetrimental effects of anabolic steroids on human endothelial cells
D’Ascenzo, S., Millimaggi, D., Massimo, C. D., Saccani‐Jotti, G., Botrè, F., Carta, G., Ciancarelli, M. G. T., Pavan, A., & Dolo, V. (2007) · Toxicology Letters · doi:10.1016/j.toxlet.2006.12.008 · Cited by 48 · see citing works
Yoshioka, M., Boivin, A., Ye, P., Labrie, F., & St‐Amand, J. (2006) · Journal of Molecular Endocrinology · doi:10.1677/jme.1.01964 · Cited by 48 · see citing works
“Androgens also have rapid non-genomic actions in the muscle ( Estrada et al. 2000, 2003 ), including membrane receptor coupled to G protein, inositol 1,4,5-trisphosphate (IP3) receptor, calcium ion (Ca2+) and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) phosphorylation cascade.”
BackgroundThe impact of reproductive hormones on T cell immunity; normal and assisted reproductive cycles
Alanazi, H., Zhang, Y., Fatunbi, J., Luu, T. V., & Kwak-Kim, J. Y.-H. (2024) · Journal of Reproductive Immunology · doi:10.1016/j.jri.2024.104295 · Cited by 51 · see citing works
Prasad, C. P., Chaurasiya, S. K., Axelsson, L., & Andersson, T. (2013) · Molecular Oncology · doi:10.1016/j.molonc.2013.04.005 · Cited by 46 · see citing works
“These results agree with previous findings in other cell types that Ca2þ positively regulates activation of ERK1/2 (Chao et al., 1992; Estrada et al., 2003;Moshal et al., 2006). rWNT-5A triggers a Ca2þ-dependent ERK activation with rapid onset and transient duration, prior to WNT-5A-induced…”
ResultAndersen, N. H., Bojesen, A., Kristensen, K., Birkebæk, N., Fedder, J., Bennett, P., Christiansen, J. S., & Gravholt, C. H. (2008) · Clinical Endocrinology · doi:10.1111/j.1365-2265.2008.03211.x · Cited by 44 · see citing works
MacLean, H. E., Moore, A., Sastra, S. A., Morris, H. A., Ghasemzadeh, A., Rana, K., Axell, A.-M., Notini, A. J., Handelsman, D. J., Seeman, E., et al. (2010) · Journal of Endocrinology · doi:10.1677/joe-10-0026 · Cited by 45 · see citing works
Buendía-González, F. O., & Legorreta‐Herrera, M. (2022) · Biomolecules · doi:10.3390/biom12121768 · Cited by 43 · see citing works
GSK-3β/NFAT Signaling Is Involved in Testosterone-Induced Cardiac Myocyte Hypertrophy
Duran, J., Oyarce, C., Pávez, M., Valladares-Ide, D., Basualto-Alarcón, C., Lagos, D., Barrientos, G. C., Troncoso, M. F., Ibarra, C., & Estrada, M. (2016) · PLoS ONE · doi:10.1371/journal.pone.0168255 · Cited by 44 · see citing works
Hulmi, J. J., Ahtiainen, J. P., Selänne, H., Volek, J. S., Häkkinen, K., Kovanen, V., & Mero, A. A. (2008) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2008.03.030 · Cited by 44 · see citing works
Therapeutic potential of the SARMs: revisiting the androgen receptor for drug discovery
Segal, S., Narayanan, R., & Dalton, J. T. (2006) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.15.4.377 · Cited by 43 · see citing works
“This rapid signalling is called nongenomic signalling of steroid receptors and has already been reported in oocytes [33], prostate cancer cells [34], osteoblasts [35] and skeletal muscle cells [36] for the AR.”
BackgroundShihan, M., Chan, K.-H., Konrad, L., & Scheiner‐Bobis, G. (2015) · Cellular Signalling · doi:10.1016/j.cellsig.2015.07.013 · Cited by 42 · see citing works
Androgen receptor as a therapeutic target
Gao, W. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.08.002 · Cited by 42 · see citing works
“In addition to the classic genomic actions, the nongenomic actions of the androgens have also been reported in various tissues, particularly the reproductive, cardiovascular, and musculoskeletal systems [8,43], and are mainly characterized in cellular models, including Sertoli cells [44,45], prostate cancer cells [46,…”
Enhanced muscle fatigue occurs in male but not female ASIC3−/− mice
Burnes, L. A., Kolker, S. J., Danielson, J. F., Walder, R. Y., & Sluka, K. A. (2008) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00687.2007 · Cited by 42 · see citing works
“Furthermore, testosterone increases intracellular calcium in myocytes producing increases in phosphorylation and transcription factors through a G proteinlinked receptor (16).”
BackgroundRosso, J. Q. D., & Kircik, L. (2024) · Journal of Dermatological Treatment · doi:10.1080/09546634.2023.2298878 · Cited by 49 · see citing works
“In addition to DNA binding–dependent (i.e., genomic) signaling, androgens can also exert their biological effects through more rapid ligand-independent (i.e., nongenomic) signaling pathways via the activation of second messenger cascades (17).”
Hamdi, M. M., & Mutungi, G. (2011) · The Journal of Physiology · doi:10.1113/jphysiol.2011.207175 · Cited by 40 · see citing works
“Previously, it has been suggested that testosterone can activate the MAPK pathway via a G-protein coupled androgen receptor (Estrada et al. 2003).”
BackgroundInfluentialThe Effects of Short-Cycle Sprints on Power, Strength, and Salivary Hormones in Elite Rugby Players
Crewther, B. T., Cook, C. J., Lowe, T. E., Weatherby, R. P., & Gill, N. (2011) · Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e3181b6045c · Cited by 38 · see citing works
“Experimental research has shown that testosterone or cortisol can produce rapid changes in intracellular Ca when applied to skeletal muscle cells (14,30), thereby potentially contributing to skeletal muscle function.”
BackgroundMembrane androgen receptors may mediate androgen reinforcement
Sato, S. M., Johansen, J. A., Jordan, C. L., & Wood, R. I. (2010) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2010.01.007 · Cited by 39 · see citing works
“, 2002), myocytes (Estrada et al., 2003), and osteoblasts (Lieberherr and Grosse, 1994).”
A novel role of Shc adaptor proteins in steroid hormone-regulated cancers
Alam, S. M., Rajendran, M., Ouyang, S., Veeramani, S., Zhang, L., & Lin, M.-F. (2008) · Endocrine Related Cancer · doi:10.1677/erc-08-0179 · Cited by 39 · see citing works
“…concentration [Ca2C] mediated via GPCR that constitutes a-, b-, and g-subunits (Lieberherr & Grosse 1994, Benten et al. 1998), which ultimately results in the rapid activation of MAPK/ERK and PI3K/AKT pathways, leading to the activation of PKC and PKA (Kelly et al. 1999, Estrada et al. 2003).”
Mechanisms Modulating Skeletal Muscle Phenotype
Blaauw, B., Schiaffino, S., & Reggiani, C. (2013) · Comprehensive physiology · doi:10.1002/j.2040-4603.2013.tb00534.x · Cited by 38 · see citing works
Sen, A., Prizant, H., & Hammes, S. R. (2011) · Steroids · doi:10.1016/j.steroids.2011.02.016 · Cited by 38 · see citing works
“B) In Sertoli cells, testosterone binds to classical ARs leading to activation of Src that promotes phosphorylation of the epidermal growth factor receptor (EGFR), resulting in activation of the MAPK pathway (Raf-MEK-Erk).”
BackgroundInfluentialBalagopal, P., Olney, R. C., Darmaun, D., Mougey, E. B., Dokler, M., Sieck, G. C., & Hammond, D. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00412.2005 · Cited by 38 · see citing works
“Nongenomic effects of the androgen receptor have also been described, including a role in calcium regulation in skeletal muscles via an inositol triphosphate-mediated pathway (12).”
Background
- Authors
- Estrada, M., Espinosa, A., Müller, M., & Jaimovich, E.
- Affiliations
- University of Chile (CL)
- Corresponding author
- Jaimovich, Enrique
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2003-08
- Volume
- 144
- Issue
- 8
- Pages
- 3586-3597
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2134223482
- Cited by
- 264
- References
- 46
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 17 · 2023: 14 · 2022: 8 · 2021: 11 · 2020: 13 · 2019: 9 · 2018: 8 · 2017: 8 · 2016: 14 · 2015: 13 · 2014: 8 · 2013: 13 · 2012: 9
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- green
- americanae.aecid.es
- Licence
- other-oa
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Olympus
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- testosterone, nandrolone, intracellular calcium release, calcium oscillations, inositol 1,4,5-trisphosphate, IP3 receptor, ERK1/2 phosphorylation, Ras/MEK/ERK signaling, G protein-coupled receptors, skeletal muscle myotubes, cyproterone, pertussis toxin, MEK inhibitors
- MeSH terms
- Androgens, Animals, Animals, Newborn, Calcium, Chelating Agents, Nandrolone, Phosphorylation, Testosterone, Transfection, Calcium Channels, Inositol 1,4,5-Trisphosphate, Gene Expression, Protein Serine-Threonine Kinases, Cyclic AMP Response Element-Binding Protein, Cyproterone Acetate, Receptors, Cytoplasmic and Nuclear, Muscle, Skeletal, Muscle Fibers, Skeletal, ras Proteins, GTP-Binding Proteins, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase 3, Rats, Inositol 1,4,5-Trisphosphate Receptors, Androgen Receptor Antagonists
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