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Cited by 128Visual perception and processing mechanismsNeurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology Research

Molecular and morphological changes in the cat lateral geniculate nucleus and visual cortex induced by visual deprivation are revealed by monoclonal antibodies Cat-304 and Cat-301

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Guimaraes, A, Zaremba, S, Hockfield, S·1990·The Journal of Neuroscience
DOI 10.1523/jneurosci.10-09-03014.1990PMC PMC6570239
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Abstract

Monoclonal antibody Cat-301 recognizes a surface-associated proteoglycan on subsets of neurons in the mammalian CNS (Hockfield and McKay, 1983). The expression of Cat-301 immunoreactivity on Y cells in the cat LGN is sharply reduced by early visual deprivation (Sur et al., 1988). We employed an immunosuppression strategy (Hockfield, 1987) to further study alterations in the expression of experience-dependent molecules. Newborn BALB/c mice were injected with LGN from dark-reared cats to induce a suppression of the immune response to antigens expressed in visually deprived animals. These mice were then immunized with LGN from normal cats to elicit an immune response to antigens with an expression dependent on normal early visual experience. This strategy permitted the generation of monoclonal antibody Cat-304, which recognizes a surface-associated antigen on neuronal cell bodies and proximal dendrites, and which appears histologically identical to Cat- 301. Further analyses show that Cat-304 and Cat-301 recognize different epitopes on the same 680-kDa chondroitin sulfate proteoglycan. We examined the effects of early visual deprivation on Cat-304 immunoreactivity in the LGN and visual cortex of cats. In LGN from normal cats, Cat-304 labels neurons in layers A, A1, and C, in interlaminar zones, and in the medial interlaminar nucleus. In LGN from dark-reared cats, the number of antibody-positive neurons is markedly reduced, and the cross-sectional area of the remaining positive neurons is smaller than normal. In cortical area 17 of normally reared cats, Cat 304-positive neurons are densely distributed in 2 bands, in layers IV and V/VI. Labeled neurons are also present in layers II and III. In area 17 of dark-reared cats, the number of antibody-positive neurons is reduced. The reduction in the number of labeled neurons is most pronounced in layers II/III and V/VI. Antibody-positive neurons are smaller in all cortical layers of dark-reared cats. The changes in the expression of Cat-301 immunoreactivity in dark-reared visual cortex and LGN are identical to those of Cat-304. The laminar differences in the effect of dark rearing on Cat-301 and Cat-304 expression in the visual cortex provides support for the suggestion that layer IV of cortical area 17 may be less susceptible to prolongation of plasticity by dark rearing than layers II/III and V/VI. Further, the biochemical and histological studies reported provide evidence that early visual experience regulates protein expression in the cat LGN and visual cortex.

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  • Aggrecan expression coincides with maturation of electrophysiological properties of neurons and formation of synapses during late stage developmental periods.

    “In parallel to PNN development, aggrecan expression coincides with the maturation of electrophysiological properties of neurons and the formation of synapses during late stage developmental periods.47, 48”

    From Aggrecan and chondroitin-6-sulfate abnormalities in schizophrenia and bipolar disorder: a postmortem study on the amygdala (2015) · cites this paper as [47]

  • PNNs regulate neural circuit functions ranging from developmental plasticity and critical-period closure to adult synaptic network stability.

    “Given that PNNs can modultate neural circuitry functions stemming from developmental plasticity and closure of the critical period (Friauf, 2000; Guimaraes et al., 1990; Pizzorusso et al., 2002, 2006)”

    From Perineuronal nets and schizophrenia: The importance of neuronal coatings (2014) · cites this paper as Guimaraes et al., 1990

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  1. DeYoe EA, Hockfield S, Garren H, Van Essen D (1990) Antibody labeling of functional subdivisions in visual cortex: Cat-301 in Vl, V2, V3 and MT of the macaque monkey. Vis Neurosci (in press).

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Authors
Guimaraes, A., Zaremba, S., & Hockfield, S.
Affiliations
Yale University (US)
ORCID
Zaremba, S
Venue
The Journal of Neuroscience
Publisher
Society for Neuroscience
Published
1990-09-01
Volume
10
Issue
9
Pages
3014-3024
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
DOI
10.1523/jneurosci.10-09-03014.1990
PMC
PMC6570239
OpenAlex
W1488311471
Cited by
128
References
22
Citations by year
2025: 2 · 2023: 4 · 2022: 5 · 2021: 4 · 2020: 4 · 2019: 1 · 2018: 8 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 6 · 2013: 4 · 2012: 6
FWCI
3.12
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • National Institutes of Health
  • National Eye Institute (EY 06511)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Visual perception and processing mechanisms
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
lateral geniculate nucleus, visual cortex, visual deprivation, dark rearing, monoclonal antibodies, chondroitin sulfate proteoglycans, experience-dependent development, Y-cells, neuronal morphology, immunosuppression, cat visual system
MeSH terms
Animals, Antibodies, Monoclonal, Epitopes, Cats, Cell Count, Darkness, Fluorescent Antibody Technique, Geniculate Bodies, Immunization, Immunosuppression Therapy, Mice, Inbred BALB C, Neurons, Proteoglycans, Visual Cortex, Blotting, Western, Mice

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