Cited by 311Neurotransmitter Receptor Influence on BehaviorNeuroscience and Neuropharmacology ResearchNeural and Behavioral Psychology Studies

Selective behavioral and neurochemical effects of cholinergic lesions produced by intrabasalis infusions of 192 IgG-saporin on attentional performance in a five-choice serial reaction time task

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McGaughy, J., Dalley, J. W., Morrison, C. H., Everitt, B. J., Robbins, T. W.·2002·The Journal of Neuroscience

Abstract

The effects of the cholinergic immunotoxin 192 IgG-saporin (SAP) (0.0, 0.15, or 0.45 microg/microl; 0.5 microl/hemisphere) infused into the area of the nucleus basalis magnocellularis (NBM) of rats were tested in a five-choice serial reaction time task (5CSRTT) designed to assess visual attention. The effects of this manipulation on acetylcholine efflux in the medial frontal cortex were determined using in vivo microdialysis during the 5CSRTT. Rats with extensive lesions of the NBM (SAP HIGH) showed an array of behavioral deficits in the 5CSRTT hypothesized to represent deficits in central executive function that were associated with severe deficits in accuracy. Lengthening the stimulus duration ameliorated these deficits. Rats with restricted lesions of the NBM (SAP LOW) showed impairments over time on task when tested under standard conditions that were exacerbated by increases in the event rate. The number of choline acetyltransferase-immunoreactive cells in the area of the NBM but not the vertical limb of the diagonal band correlated significantly with accuracy in the task. SAP HIGH rats had significantly lower levels of cortical acetylcholine (ACh) efflux relative to SHAM both before and during the 5CSRTT. SAP LOW rats showed significantly higher levels of cortical ACh efflux before but not during the 5CSRTT. Cortical ACh efflux increased in all rats with the onset of the attentional task. These data provide the first direct evidence for a relationship between selective damage in the basal forebrain with decreased cortical ACh efflux and impaired attentional function.

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Crossref · 10.1523/jneurosci.22-05-01905.2002

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  • In rodents, lesions of cholinergic inputs to the cortex impair tests of sustained attention, particularly across sensory modalities.

    “In rodents, lesions of cholinergic inputs to the cortex impair tests of sustained attention, particularly across sensory modalities (McGaughy et al., 2002; McGaughy et al., 1996; Turchi and Sarter, 1997).”

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  1. Zar JH (1974) Biostatistical analyses. Englewood Cliffs, NJ: Prentice Hall.

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Authors
McGaughy, J., Dalley, J. W., Morrison, C. H., Everitt, B. J., & Robbins, T. W.
Affiliations
University of Cambridge (GB)
Venue
The Journal of Neuroscience
Published
2002-03-01
Volume
22
Issue
5
Pages
1905-1913
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
OpenAlex
W1945955617
Cited by
311
References
54
Citations by year
2026: 7 · 2025: 4 · 2024: 9 · 2023: 7 · 2022: 11 · 2021: 6 · 2020: 10 · 2019: 8 · 2018: 13 · 2017: 13 · 2016: 9 · 2015: 11 · 2014: 12 · 2013: 20 · 2012: 9
FWCI
6.62
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust
  • Medical Research Council
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural and Behavioral Psychology Studies
Keywords
nucleus basalis magnocellularis, 192 IgG-saporin, cholinergic lesions, acetylcholine efflux, visual attention, 5-choice serial reaction time task, choline acetyltransferase, central executive function, basal forebrain, medial frontal cortex
MeSH terms
Saporins, Acetylcholine, Animals, Antibodies, Monoclonal, Immunotoxins, Attention, Behavior, Animal, Cell Count, Choice Behavior, Choline O-Acetyltransferase, Drug Administration Routes, Frontal Lobe, Male, Neurons, N-Glycosyl Hydrolases, Rats, Inbred Strains, Reaction Time, Microdialysis, Basal Nucleus of Meynert, Rats, Ribosome Inactivating Proteins, Type 1

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