Cited by 137Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyMycotoxins in Agriculture and Food

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index; 4 of the 27 sources its claims cite were checked for support.

The Effect of Treating Whole-Plant Barley with Lactobacillus buchneri 40788 on Silage Fermentation, Aerobic Stability, and Nutritive Value for Dairy Cows

Open access
Taylor, C.C., Ranjit, N.J., Mills, J.A., Neylon, J.M., Kung, L.·2002·Journal of Dairy Science

Abstract

Chopped barley forage was ensiled untreated or treated with several doses (1 x 10(5) to 1 x 10(6) cfu/g of fresh forage) of Lactobacillus buchneri 40788 in laboratory silos and untreated or treated (4 x 10(5) cfu/g) in a farm silo. Silage from the farm silos was fed to lactating cows. In the laboratory silo, the effects of inoculation on fermentation and aerobic stability were also compared to silage treated with a commercial inoculant and a buffered propionic acid additive. Inoculation with L. buchneri 40788 decreased the final concentrations of lactic acid but increased concentrations of acetic acid and ethanol in silage from laboratory and farm silos. Silages stored in laboratory silos did not heat after exposure to air for 7 d and were then mixed with alfalfa silage and a concentrate to form total mixed rations (TMR) that were further exposed to air. The TMR containing silages treated with L. buchneri 40788 or a buffered propionic-acid-based additive took longer to heat and spoil than the TMR containing untreated silage or silagetreated with the commercial inoculant. Silage stored in a farm silo and treated with L. buchneri 40788 had fewer yeasts and molds than did untreated silage. Aerobic stability was greater in treated silage alone and in a TMR containing treated silage. Dry matter intake (18.6 kg/d), milk production (25.7 kg/d), and milk composition did not differ between cows fed a TMR containinguntreated or treated silage. These findings show that L. buchneri can improve the aerobic stability of barley silage in laboratory and farm silos and that feeding treated silage had no negative effect on intake or performance.

Overview

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TL;DR

Treating whole-plant barley silage with Lactobacillus buchneri 40788 increased acetic acid and ethanol, reduced lactic acid, improved aerobic stability in laboratory and farm silos, and had no negative effect on intake or milk production in lactating dairy cows. The highest laboratory dose was most effective for TMR stability, while farm-treated silage had fewer yeasts and longer stability than untreated.

Key findings

  • In laboratory silos after 120 d, L. buchneri 40788 lowered lactic acid (control 3.53% vs 2.11–2.51% for LB1–LB3) and raised acetic acid (control 2.48% vs 4.06–4.98%) and ethanol (control 0.43% vs average 0.91% for treated); propionic acid was greater for LB2 (1.29%) and LB3 (1.44%) than control (0.28%).
  • Laboratory silages did not heat after 7 d air exposure, but TMR containing L. buchneri-treated barley was more stable than TMR with untreated silage (95 h); BPA was 105 h and LB1/LB2 averaged 106 h, with the highest dose most effective.
  • In the farm silo after 10 mo, treated barley silage had fewer yeasts (2.63 vs 4.89 log10 cfu/g) and greater aerobic stability (190 h vs 66 h) than untreated; TMR stability was also greater with treated silage.
  • Lactation study: DMI (18.6 kg/d), milk production (25.7 kg/d), and milk composition did not differ between cows fed TMR containing untreated vs treated barley silage.

Methods

  • Laboratory silo study: chopped whole-plant barley wilted to ~36% DM, ensiled in macro silos in triplicate with untreated, L. buchneri 40788 at 1 × 10^5, 5 × 10^5, or 1 × 10^6 cfu/g plus enzymes, a commercial inoculant, or a buffered propionic acid additive; stored 120 d at 20–27°C, then analyzed for fermentation, microbes, DM recovery, and aerobic stability alone and in TMR.
  • Lactation study: barley harvested at early heading, wilted to ~40% DM, ensiled untreated or treated with L. buchneri 40788 at 4 × 10^5 cfu/g in a bag silo; after 10 mo, 20 Holstein cows (14 multiparous, 6 primiparous; average 25 kg milk/d, 184 ± 28 DIM) were used in a crossover design with two 4-wk periods, last 2 wk collection, fed TMR with 35% untreated or treated barley silage, 15% corn silage, 50% concentrate.
  • Analyses: microbial data log10-transformed; chemical data on DM basis; ANOVA via SAS GLM, Tukey’s test, P < 0.05.

Limitations

  • Stated: the lactation study was designed to evaluate potential negative effects of feeding L. buchneri-treated silage during moderate weather (22–28°C), not to show positive effects under severely hot weather and/or poor management.
  • Stated: 1,2-propanediol was not measured, and some findings (e.g., propionic acid increases, lower starch/CP in LB2) were unexplained.
  • Evident: the farm-scale silo comparison used one bag silo split into untreated and treated halves rather than replicated farm silos; laboratory silages did not heat after 7 d, so TMR stability was assessed using alfalfa silage with high yeast/mold counts (5.17 and 3.00 log10 cfu/g) that spoiled after 124 h.

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Integrity signals

Registry

Crossref · 10.3168/jds.s0022-0302(02)74253-7

Cites

24 of 24 references verified0 citation errors · 0 not checkable

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Claims the paper makes

62 Own result · 15 Not checked yet · 6 No basis given · 5 Own interpretation · 3 Own method · 2 Partly supported · accuracy not yet measured

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Show all 73 claims

  • It may state two separate claims as one. 8
  • Probably not a substantial claim. 13
  • The quote only partly states this claim. 1
20 of 20
  • The paper relies on this without support.

    Bunker silos result in considerable amounts of silage that are exposed to air.

    “However, these structures result in considerable amounts of silage that are exposed to air”
  • The paper relies on this without support.

    Complaints related to the poor aerobic stability of silages from bunker silos have increased.

    “complaints related to the poor aerobic stability of silages from these types of silos have increased.”
  • The paper relies on this without support.

    Unless silages are packed tightly and managed well during removal, infiltration of air into the silage mass can stimulate the growth of aerobic microbes and cause silage to spoil quickly.

    “Unless the silages are packed tightly and managed well during removal from the silo, infiltration of air into the silage mass can stimulate the growth of aerobic microbes and cause silage to spoil quickly.”
  • The paper relies on this without support.

    Lactobacillus buchneri inhibits the proliferation of yeasts in silage via the production of acetic acid.

    “Lactobacillus buchneri has been shown to inhibit the proliferation of yeasts in silage via the production of acetic acid.”
  • The paper relies on this without support.

    Higher concentrations of acetic acid inhibit yeasts.

    “Thus, silage treated with L. buchneri 40788 often has a moderately high concentration of ethanol despite having lower numbers of yeasts, which are inhibited by the higher concentration of acetic acid.”
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3 of 3

Citations

Cites

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Research works printed but not linked: 2

  1. Hoffman, 1997, Quantification of milk yield losses associated with feeding aerobically unstable high moisture corn, J. Dairy Sci., 80, 234
  2. Whitlock, 2000, Effect of level of surface-spoiled silage on the nutritive value of corn silage diets, J. Dairy Sci., 83, 110

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Metadata

Authors
Taylor, C. C., Ranjit, N. J., Mills, J. A., Neylon, J. M., & Kung, L.
Affiliations
University of Delaware (US)
Corresponding author
Kung, L.
Venue
Journal of Dairy Science
Published
2002-07
Volume
85
Issue
7
Pages
1793-1800
ISSN
0022-0302 (ISSN-L)
OpenAlex
W1993794810
Cited by
137
References
24
Citations by year
2026: 2 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 4 · 2020: 12 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 3 · 2015: 9 · 2014: 7 · 2013: 3 · 2012: 10
FWCI
9.72
Type
journal article
Language
en
Open access
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
Keywords
barley silage, silage inoculation, aerobic stability, silage fermentation, lactic acid, acetic acid, ethanol, silage spoilage, molds and yeasts, silage inoculants, total mixed ration, dairy cattle, dry matter intake, milk production, milk composition
MeSH terms
Aerobiosis, Animals, Animal Feed, Animal Nutritional Physiological Phenomena, Hordeum, Cattle, Female, Fermentation, Food Preservation, Lactation, Lactobacillus, Nutritive Value, Propionates, Silage, Colony Count, Microbial, Acetic Acid

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