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Cited by 643Lymphatic System and DiseasesCerebrospinal fluid and hydrocephalusLymphatic Disorders and Treatments

The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.

Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI.

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Absinta, Martina, Ha, Seung-Kwon, Nair, Govind, Sati, Pascal, Luciano, Nicholas J, Palisoc, Maryknoll, Louveau, Antoine, Zaghloul, Kareem A, Pittaluga, Stefania, Kipnis, Jonathan, Reich, Daniel S·2017·eLife
DOI 10.7554/elife.29738PMC PMC5626482
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Abstract

Here, we report the existence of meningeal lymphatic vessels in human and nonhuman primates (common marmoset monkeys) and the feasibility of noninvasively imaging and mapping them in vivo with high-resolution, clinical MRI. On T2-FLAIR and T1-weighted black-blood imaging, lymphatic vessels enhance with gadobutrol, a gadolinium-based contrast agent with high propensity to extravasate across a permeable capillary endothelial barrier, but not with gadofosveset, a blood-pool contrast agent. The topography of these vessels, running alongside dural venous sinuses, recapitulates the meningeal lymphatic system of rodents. In primates, meningeal lymphatics display a typical panel of lymphatic endothelial markers by immunohistochemistry. This discovery holds promise for better understanding the normal physiology of lymphatic drainage from the central nervous system and potential aberrations in neurological diseases.

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Figures

Figure 1. MRI-visualization of dural lymphatic vessels in human and nonhuman primates. In both species, conventional post-gadobutrol coronal T1-weighted MRI is unable to discriminate lymphatic vessels due to diffuse physiological enhancement of the dura (arrows) and blood vessels, including the superior sagittal sinus and straight sinus (arrows). On post-gadobutrol coronal T2-FLAIR and subtraction images, the dura does not enhance, and lymphatic vessels (red arrows), running alongside the venous dural sinuses and within the falx cerebri, can be appreciated. Numbers refer to minutes after the intravenous administration of gadobutrol.
Figure 1. MRI-visualization of dural lymphatic vessels in human and nonhuman primates. In both species, conventional post-gadobutrol coronal T1-weighted MRI is unable to discriminate lymphatic vessels due to diffuse physiological enhancement of the dura (arrows) and blood vessels, including the superior sagittal sinus and straight sinus (arrows). On post-gadobutrol coronal T2-FLAIR and subtraction images, the dura does not enhance, and lymphatic vessels (red arrows), running alongside the venous dural sinuses and within the falx cerebri, can be appreciated. Numbers refer to minutes after the intravenous administration of gadobutrol.CC0 · PubMed Central
Figure 1—figure supplement 1. 3D rendering of human dural lymphatics. 3D rendering of putative dural lymphatics (black) in a 47 year old woman, derived from skull-stripped subtraction T1-black-blood images (same case as in Video 1). Thumbnails are 3D renderings of the brain parenchyma to aid visualization of image orientation.
Figure 1—figure supplement 1. 3D rendering of human dural lymphatics. 3D rendering of putative dural lymphatics (black) in a 47 year old woman, derived from skull-stripped subtraction T1-black-blood images (same case as in Video 1). Thumbnails are 3D renderings of the brain parenchyma to aid visualization of image orientation.CC0 · PubMed Central
Figure 2. Gadobutrol vs. gadofosveset in MRI-visualization of dural lymphatic vessels. Coronal T1-weighted black-blood images were acquired after intravenous injection of two different gadolinium-based contrast agents (31 min after gadobutrol and 42 min after gadofosveset), during two MRI sessions separated by one week. Dural lymphatics (red arrows in magnified view boxes) were better discerned using gadobutrol (standard MRI contrast agent, which readily enters the dura) compared to gadofosveset (serum albumin-binding contrast agent, which remains largely intravascular) and were localized around dural sinuses, middle meningeal artery, and cribriform plate (white arrows). Notably, the choroid plexus (white arrows) enhanced less with gadofosveset than gadobutrol, whereas meningeal and parenchymal blood vessels (both veins and arteries) did not enhance with any contrast agent and appeared black. On conventional T1-weighted MPRAGE images, meningeal and parenchymal blood vessels enhanced with both contrast agents, more clearly with gadofosveset.
Figure 2. Gadobutrol vs. gadofosveset in MRI-visualization of dural lymphatic vessels. Coronal T1-weighted black-blood images were acquired after intravenous injection of two different gadolinium-based contrast agents (31 min after gadobutrol and 42 min after gadofosveset), during two MRI sessions separated by one week. Dural lymphatics (red arrows in magnified view boxes) were better discerned using gadobutrol (standard MRI contrast agent, which readily enters the dura) compared to gadofosveset (serum albumin-binding contrast agent, which remains largely intravascular) and were localized around dural sinuses, middle meningeal artery, and cribriform plate (white arrows). Notably, the choroid plexus (white arrows) enhanced less with gadofosveset than gadobutrol, whereas meningeal and parenchymal blood vessels (both veins and arteries) did not enhance with any contrast agent and appeared black. On conventional T1-weighted MPRAGE images, meningeal and parenchymal blood vessels enhanced with both contrast agents, more clearly with gadofosveset.CC0 · PubMed Central
Figure 2—figure supplement 1. MRI visualization of dural lymphatic vessels in a healthy marmoset with different contrast agents. In an 11-year-old healthy common marmoset, high-resolution (150 × 150 μm in-plane) coronal T1-weighted MPRAGE and T2-FLAIR were acquired before and at different time points after the injection of two different contrast agents (single dose of gadobutrol and gadofosveset), a week apart. (1) Gadobutrol experiment. Dural lymphatic vessels are discriminated on postcontrast T2-FLAIR and on subtraction images (post vs. precontrast images), but not on postcontrast 3D T1-weighted MPRAGE. (2) Gadofosveset experiment. Gadofosveset, which binds serum albumin and therefore remains largely inside blood vessels (white arrows on postcontrast T1-MPRAGE and subtraction images), even in tissues (such as the dura mater) that lack a blood-brain barrier, is FDA-approved for magnetic resonance angiography. Dural lymphatic vessels are not visible on post-gadofosveset T2-FLAIR and relative subtraction images, even on delayed acquisition (45 min after injection), despite preserved enhancement of the dural venous sinuses. Numbers refer to minutes after intravenous administration of the contrast agent.
Figure 2—figure supplement 1. MRI visualization of dural lymphatic vessels in a healthy marmoset with different contrast agents. In an 11-year-old healthy common marmoset, high-resolution (150 × 150 μm in-plane) coronal T1-weighted MPRAGE and T2-FLAIR were acquired before and at different time points after the injection of two different contrast agents (single dose of gadobutrol and gadofosveset), a week apart. (1) Gadobutrol experiment. Dural lymphatic vessels are discriminated on postcontrast T2-FLAIR and on subtraction images (post vs. precontrast images), but not on postcontrast 3D T1-weighted MPRAGE. (2) Gadofosveset experiment. Gadofosveset, which binds serum albumin and therefore remains largely inside blood vessels (white arrows on postcontrast T1-MPRAGE and subtraction images), even in tissues (such as the dura mater) that lack a blood-brain barrier, is FDA-approved for magnetic resonance angiography. Dural lymphatic vessels are not visible on post-gadofosveset T2-FLAIR and relative subtraction images, even on delayed acquisition (45 min after injection), despite preserved enhancement of the dural venous sinuses. Numbers refer to minutes after intravenous administration of the contrast agent.CC0 · PubMed Central
Figure 3. Histopathology of human dural lymphatic vessels. (A–B) Whole-mount and coronal views of the human dura mater before sampling for histological analysis. The dotted line in A shows where the superior sagittal sinus runs within the dural layers. (C) Coronal section of human dura stained with H and E to highlight anatomical features of interest, including the falx cerebri and the dural venous sinus. Note the distortion of the dura after paraffin embedding in comparison to B. (D, F, G) Within the dura mater, lymphatic and blood vessels can be differentiated using double immunostaining for PROX1 (a transcription factor involved in lymphangiogenesis, nuclear staining) and CD31 (a vascular endothelial cell marker). (E, H–K) Similarly, lymphatic and blood vessels can be differentiated using immunohistochemical (E, H, I) and immunofluorescent (J, K) double staining for podoplanin (D2-40, endothelial membrane staining) and CD31. Red blood cells are seen within blood vessels, but not within lymphatic vessels. Insets (F–I) were rotated relative to the original figures in D and E. Abbreviations: H and E: hematoxylin and eosin; LV: lymphatic vessels; BV: blood vessels.
Figure 3. Histopathology of human dural lymphatic vessels. (A–B) Whole-mount and coronal views of the human dura mater before sampling for histological analysis. The dotted line in A shows where the superior sagittal sinus runs within the dural layers. (C) Coronal section of human dura stained with H and E to highlight anatomical features of interest, including the falx cerebri and the dural venous sinus. Note the distortion of the dura after paraffin embedding in comparison to B. (D, F, G) Within the dura mater, lymphatic and blood vessels can be differentiated using double immunostaining for PROX1 (a transcription factor involved in lymphangiogenesis, nuclear staining) and CD31 (a vascular endothelial cell marker). (E, H–K) Similarly, lymphatic and blood vessels can be differentiated using immunohistochemical (E, H, I) and immunofluorescent (J, K) double staining for podoplanin (D2-40, endothelial membrane staining) and CD31. Red blood cells are seen within blood vessels, but not within lymphatic vessels. Insets (F–I) were rotated relative to the original figures in D and E. Abbreviations: H and E: hematoxylin and eosin; LV: lymphatic vessels; BV: blood vessels.CC0 · PubMed Central
Figure 3—figure supplement 1. Histopathology of human dural lymphatic vessels. Lymphatic vessels (brown, arrows) in different samples of human dura mater visualized with double immunostaining (PROX1/CD31, D2-40 podoplanin/CD31, PROX1/D2-40 podoplanin).
Figure 3—figure supplement 1. Histopathology of human dural lymphatic vessels. Lymphatic vessels (brown, arrows) in different samples of human dura mater visualized with double immunostaining (PROX1/CD31, D2-40 podoplanin/CD31, PROX1/D2-40 podoplanin).CC0 · PubMed Central
Figure 3—figure supplement 2. Positive and negative control staining for lymphatic vessels. (A–F) 5 μm paraffin sections of the human skin were implemented as positive controls for lymphatic endothelium cell marker assessment. (G–I) Negative control staining (only secondary antibodies, DAB development, and hematoxylin) of 5 μm paraffin sections of human skin, human dura mater, and marmoset brain, respectively. No background staining or nonspecific binding was seen in any tissue.
Figure 3—figure supplement 2. Positive and negative control staining for lymphatic vessels. (A–F) 5 μm paraffin sections of the human skin were implemented as positive controls for lymphatic endothelium cell marker assessment. (G–I) Negative control staining (only secondary antibodies, DAB development, and hematoxylin) of 5 μm paraffin sections of human skin, human dura mater, and marmoset brain, respectively. No background staining or nonspecific binding was seen in any tissue.CC0 · PubMed Central
Figure 4. Histopathology of dural lymphatic vessels in common marmosets. MRI-histopathological correlation in a 4.4-year-old marmoset (A–F). (A, B) Postcontrast coronal T2-FLAIR showing three enhancing lymphatic vessels within the dura, and corresponding H and E section for anatomical reference (area of interest in the box). (C-F) Three clusters of cells (circles), surrounding the SSS and positive for lymphatic endothelial cell markers, correspond to the three enhancing areas seen on MRI (A). LYVE-1 is a lymphatic endothelial cell marker (membrane staining), PROX1 and COUP-TFII are transcription factors involved in lymphangiogenesis (nuclear staining), and CCL21 is a chemokine implicated in lymphatic transmigration. Higher magnifications are shown in Figure 4—figure supplement 1. 10.3-year-old marmoset (G-I). (G) H and E coronal section showing the brain parenchyma, meninges, and area of interest for lymphatics for anatomical reference (box). This animal did not recover after undergoing general anesthesia for blood tuberculosis testing, and at necropsy a stroke was identified in one hemisphere (asterisk). (H-I) Lymphatic (circles) and blood vessels were differentiated using double staining for podoplanin (D2-40, endothelial membrane staining) and vascular endothelial cell marker (CD31), respectively. 3.7-year-old marmoset (J-L). (J) Whole-mount of the marmoset dura, including the SSS. The high level of vascularization of the dura can be appreciated. The arrow indicates the area shown in K and L. (K, L) Double staining for podoplanin D2-40 for lymphatics, and CD31 for vascular endothelial cells, show the presence of a linear vascular structure parallel to the SSS, positive for podoplanin D2-40 but not CD31. The SSS is positive for both markers, probably because of antibody entrapment during immunofluorescence staining of the whole-mount dura. Abbreviations: H and E: hematoxylin and eosin; LV: lymphatic vessels; SSS: superior sagittal sinus.
Figure 4. Histopathology of dural lymphatic vessels in common marmosets. MRI-histopathological correlation in a 4.4-year-old marmoset (A–F). (A, B) Postcontrast coronal T2-FLAIR showing three enhancing lymphatic vessels within the dura, and corresponding H and E section for anatomical reference (area of interest in the box). (C-F) Three clusters of cells (circles), surrounding the SSS and positive for lymphatic endothelial cell markers, correspond to the three enhancing areas seen on MRI (A). LYVE-1 is a lymphatic endothelial cell marker (membrane staining), PROX1 and COUP-TFII are transcription factors involved in lymphangiogenesis (nuclear staining), and CCL21 is a chemokine implicated in lymphatic transmigration. Higher magnifications are shown in Figure 4—figure supplement 1. 10.3-year-old marmoset (G-I). (G) H and E coronal section showing the brain parenchyma, meninges, and area of interest for lymphatics for anatomical reference (box). This animal did not recover after undergoing general anesthesia for blood tuberculosis testing, and at necropsy a stroke was identified in one hemisphere (asterisk). (H-I) Lymphatic (circles) and blood vessels were differentiated using double staining for podoplanin (D2-40, endothelial membrane staining) and vascular endothelial cell marker (CD31), respectively. 3.7-year-old marmoset (J-L). (J) Whole-mount of the marmoset dura, including the SSS. The high level of vascularization of the dura can be appreciated. The arrow indicates the area shown in K and L. (K, L) Double staining for podoplanin D2-40 for lymphatics, and CD31 for vascular endothelial cells, show the presence of a linear vascular structure parallel to the SSS, positive for podoplanin D2-40 but not CD31. The SSS is positive for both markers, probably because of antibody entrapment during immunofluorescence staining of the whole-mount dura. Abbreviations: H and E: hematoxylin and eosin; LV: lymphatic vessels; SSS: superior sagittal sinus.CC0 · PubMed Central
Figure 4—figure supplement 1. Histopathology of marmoset dural lymphatic vessels. High magnification of insets C-F from Figure 4.
Figure 4—figure supplement 1. Histopathology of marmoset dural lymphatic vessels. High magnification of insets C-F from Figure 4.CC0 · PubMed Central

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  1. Mascagni, 1816, Istoria Completa Dei Vasi Linfatici, Vol. II, 195

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Metadata

Authors
Absinta, M., Ha, S.-K., Nair, G., Sati, P., Luciano, N. J., Palisoc, M., Louveau, A., Zaghloul, K. A., Pittaluga, S., Kipnis, J., & Reich, D. S.
Affiliations
  • National Institutes of Health (US)
  • University of Virginia (US)
Corresponding author
Reich, Daniel S
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2017-10-03
Volume
6
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.29738
PMC
PMC5626482
OpenAlex
W2760815599
Cited by
643
References
32
Citations by year
2026: 61 · 2025: 88 · 2024: 73 · 2023: 77 · 2022: 79 · 2021: 78 · 2020: 70 · 2019: 60 · 2018: 51 · 2017: 4
FWCI
22.31
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institute of Neurological Disorders and Stroke (Intramural Research Program)
  • National Multiple Sclerosis Society (Postdoctoral Fellowship)
  • Conrad N. Hilton Foundation (Marilyn Hilton Award for Innovation in Multiple Sclerosis Research)
  • National Cancer Institute (Intramural Research Program)
  • National Institute on Aging (R01AG034113)
  • Lymphatic Education and Research Network (Postdoctoral fellowship)
Topics
  • Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
  • Health Sciences › Medicine › Clinical Neurology › Cerebrospinal fluid and hydrocephalus
  • Health Sciences › Medicine › Surgery › Lymphatic Disorders and Treatments
Keywords
meningeal lymphatic vessels, meningeal lymphatic system, non-human primates, Callithrix jacchus, human brain, gadobutrol, gadofosveset, contrast extravasation, lymphatic endothelial markers, dural venous sinuses, black-blood imaging
MeSH terms
Animals, Callithrix, Humans, Magnetic Resonance Imaging, Meninges, Lymphatic Vessels

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