The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Neural stem and progenitor cells in nestin‐GFP transgenic mice
Open accessAbstract
Neural stem cells generate a wide spectrum of cell types in developing and adult nervous systems. These cells are marked by expression of the intermediate filament nestin. We used the regulatory elements of the nestin gene to generate transgenic mice in which neural stem cells of the embryonic and adult brain are marked by the expression of green fluorescent protein (GFP). We used these animals as a reporter line for studying neural stem and progenitor cells in the developing and adult nervous systems. In these nestin‐GFP animals, we found that GFP‐positive cells reflect the distribution of nestin‐positive cells and accurately mark the neurogenic areas of the adult brain. Nestin‐GFP cells can be isolated with high purity by using fluorescent‐activated cell sorting and can generate multipotential neurospheres. In the adult brain, nestin‐GFP cells are ∼1,400‐fold more efficient in generating neurospheres than are GFP‐negative cells and, despite their small number, give rise to 70 times more neurospheres than does the GFP‐negative population. We characterized the expression of a panel of differentiation markers in GFP‐positive cells in the nestin‐GFP transgenics and found that these cells can be divided into two groups based on the strength of their GFP signal: GFP‐bright cells express glial fibrillary acidic protein (GFAP) but not βIII‐tubulin, whereas GFP‐dim cells express βIII‐tubulin but not GFAP. These two classes of cells represent distinct classes of neuronal precursors in the adult mammalian brain, and may reflect different stages of neuronal differentiation. We also found unusual features of nestin‐GFP–positive cells in the subgranular cell layer of the dentate gyrus. Together, our results indicate that GFP‐positive cells in our transgenic animals accurately represent neural stem and progenitor cells and suggest that these nestin‐GFP–expressing cells encompass the majority of the neural stem cells in the adult brain. J. Comp. Neurol. 469:311–324, 2004. © 2004 Wiley‐Liss, Inc.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Gage, F. H. (2000) · Science · doi:10.1126/science.287.5457.1433 · Cited by 4,786
Subventricular zone astrocytes are neural stem cells in the adult mammalian brain
Doetsch, F., Caillé, I., Lim, D. A., García-Verdugo, J. M., & Alvarez-Buylla, A. (1999) · Cell · doi:10.1016/s0092-8674(00)80783-7 · Cited by 3,898
Kuhn, H., Dickinson-Anson, H., & Gage, F. (1996) · The Journal of Neuroscience · doi:10.1523/jneurosci.16-06-02027.1996 · Cited by 3,139
Astrocytes give rise to new neurons in the adult mammalian hippocampus
Seri, B., Garcı́a-Verdugo, J. M., McEwen, B. S., & Alvarez-Buylla, A. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-18-07153.2001 · Cited by 1,495
Morshead, C. M., Reynolds, B. A., Craig, C. G., McBurney, M. W., Staines, W. A., Morassutti, D., Weiss, S., & van der Kooy, D. (1994) · Neuron · doi:10.1016/0896-6273(94)90046-9 · Cited by 1,403
Cited by
Microglia Shape Adult Hippocampal Neurogenesis through Apoptosis-Coupled Phagocytosis
Sierra, A., Encinas, J. M., Deudero, J. J. P., Chancey, J. H., Enikolopov, G., Overstreet-Wadiche, L. S., Tsirka, S. E., & Maletic-Savatic, M. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.014 · Cited by 1,622
Self-citationZhou, B. O., Yue, R., Murphy, M. M., Peyer, J. G., & Morrison, S. J. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.06.008 · Cited by 1,381
“…2010), Rosa26-loxp-stop-loxp-EYFP (Srinivas et al., 2001), Rosa26-loxp-stop-loxp-iDTR (Buch et al., 2005), NG2-creERTM (Zhu et al., 2011), Wnt-1-cre (Danielian and McMahon, 1996), Nestin-GFP (Mignone et al., 2004), Nestin-creER (Balordi and Fishell, 2007), Mx-1-cre (Kühn et al., 1995), and Col2.”
Direct measurement of local oxygen concentration in the bone marrow of live animals
Spencer, J. A., Ferraro, F., Roussakis, E., Klein, A., Wu, J., Runnels, J. M., Zaher, W., Mortensen, L. J., Alt, C., Turcotte, R., et al. (2014) · Nature · doi:10.1038/nature13034 · Cited by 1,167
Single-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis
Shin, J., Berg, D. A., Zhu, Y., Shin, J. Y., Song, J., Bonaguidi, M. A., Enikolopov, G., Nauen, D. W., Christian, K. M., Ming, G.-L., et al. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.07.013 · Cited by 784
Self-citationCoupled Proliferation and Apoptosis Maintain the Rapid Turnover of Microglia in the Adult Brain
Askew, K., Li, K., Olmos-Alonso, A., Garcia-Moreno, F., Liang, Y., Richardson, P., Tipton, T., Chapman, M. A., Riecken, K., Beccari, S., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2016.12.041 · Cited by 726
Fluoxetine targets early progenitor cells in the adult brain
Encinas, J. M., Vaahtokari, A., & Enikolopov, G. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601992103 · Cited by 637
Self-citationRegulation and function of adult neurogenesis: from genes to cognition.
Aimone, J. B., Li, Y., Lee, S. W., Clemenson, G. D., Deng, W., & Gage, F. H. (2014) · Physiological Reviews · doi:10.1152/physrev.00004.2014 · Cited by 635
Deng, W., Saxe, M. D., Gallina, I. S., & Gage, F. H. (2009) · The Journal of Neuroscience · doi:10.1523/jneurosci.3362-09.2009 · Cited by 493
Physical exercise prevents age-related decline in precursor cell activity in the mouse dentate gyrus
Kronenberg, G., Bick-Sander, A., Bunk, E., Wolf, C., Ehninger, D., & Kempermann, G. (2006) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2005.09.016 · Cited by 477
Sleep modulates haematopoiesis and protects against atherosclerosis
McAlpine, C. S., Kiss, M. G., Rattik, S., He, S., Vassalli, A., Valet, C., Anzai, A., Chan, C. T., Mindur, J. E., Kahles, F., et al. (2019) · Nature · doi:10.1038/s41586-019-0948-2 · Cited by 447
Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms
Arranz, L., Sánchez-Aguilera, A., Martín-Pérez, D., Isern, J., Langa, X., Tzankov, A., Lundberg, P., Muntión, S., Tzeng, Y.-S., Lai, D.-M., et al. (2014) · Nature · doi:10.1038/nature13383 · Cited by 444
Hanoun, M., Zhang, D., Mizoguchi, T., Pinho, S., Pierce, H., Kunisaki, Y., Lacombe, J., Armstrong, S. A., Dührsen, U., & Frenette, P. S. (2014) · Cell stem cell · doi:10.1016/j.stem.2014.06.020 · Cited by 381
Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells
Furutachi, S., Miya, H., Watanabe, T., Kawai, H., Yamasaki, N., Harada, Y., Imayoshi, I., Nelson, M., Nakayama, K. I., Hirabayashi, Y., et al. (2015) · Nature Neuroscience · doi:10.1038/nn.3989 · Cited by 329
Ho, Y.-H., del Toro, R., Rivera-Torres, J., Rak, J., Korn, C., García-García, A., Macías, D., González-Gómez, C., del Monte, A., Wittner, M., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.06.007 · Cited by 315
Frodermann, V., Rohde, D., Courties, G., Severe, N., Schloss, M. J., Amatullah, H., McAlpine, C. S., Cremer, S., Hoyer, F. F., Ji, F., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0633-x · Cited by 292
Artegiani, B., Lyubimova, A., Muraro, M., van Es, J. H., van Oudenaarden, A., & Clevers, H. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.050 · Cited by 259
Day, K., Shefer, G., Shearer, A., & Yablonka-Reuveni, Z. (2010) · Developmental Biology · doi:10.1016/j.ydbio.2010.01.006 · Cited by 254
p53-Dependent Nestin Regulation Links Tumor Suppression to Cellular Plasticity in Liver Cancer
Tschaharganeh, D. F., Xue, W., Calvisi, D. F., Evert, M., Michurina, T. V., Dow, L. E., Banito, A., Katz, S. F., Kastenhuber, E. R., Weissmueller, S., et al. (2014) · Cell · doi:10.1016/j.cell.2014.05.051 · Cited by 227
Self-citationCSF‐1 Controls Cerebellar Microglia and Is Required for Motor Function and Social Interaction
Kana, V., Desland, F. A., Casanova-Acebes, M., Ayata, P., Badimon, A., Nabel, E., Yamamuro, K., Sneeboer, M., Tan, I.-L., Flanigan, M. E., et al. (2019) · Journal of Experimental Medicine · doi:10.1084/jem.20182037 · Cited by 220
Keyes, W. M., Pecoraro, M., Aranda, V., Vernersson-Lindahl, E., Li, W., Vogel, H., Guo, X., Garcia, E. L., Michurina, T. V., Enikolopov, G., et al. (2011) · Cell Stem Cell · doi:10.1016/j.stem.2010.12.009 · Cited by 193
Self-citationCircumventricular organs: A novel site of neural stem cells in the adult brain
Bennett, L., Yang, M., Enikolopov, G., & Iacovitti, L. (2009) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2009.04.007 · Cited by 186
Self-citationHarris, L., Rigo, P., Stiehl, T., Gaber, Z. B., Austin, S. H. L., Masdeu, M. D. M., Edwards, A., Urbán, N., Marciniak-Czochra, A., & Guillemot, F. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.01.003 · Cited by 173
Walgrave, H., Balusu, S., Snoeck, S., Vanden Eynden, E., Craessaerts, K., Thrupp, N., Wolfs, L., Horré, K., Fourne, Y., Ronisz, A., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.05.001 · Cited by 175
Cipriani, S., Ferrer, I., Aronica, E., Kovacs, G. G., Verney, C., Nardelli, J., Khung, S., Delezoide, A.-L., Milenkovic, I., Rasika, S., et al. (2018) · Cerebral Cortex · doi:10.1093/cercor/bhy096 · Cited by 169
PDGFRα signaling drives adipose tissue fibrosis by targeting progenitor cell plasticity
Iwayama, T., Steele, C., Yao, L., Dozmorov, M. G., Karamichos, D., Wren, J. D., & Olson, L. E. (2015) · Genes & Development · doi:10.1101/gad.260554.115 · Cited by 164
Bone marrow NG2+/Nestin+ mesenchymal stem cells drive DTC dormancy via TGF-β2
Nobre, A. R., Risson, E., Singh, D. K., Di Martino, J. S., Cheung, J. F., Wang, J., Johnson, J., Russnes, H. G., Bravo-Cordero, J. J., Birbrair, A., et al. (2021) · Nature Cancer · doi:10.1038/s43018-021-00179-8 · Cited by 159
Zhao, M., Tao, F., Venkatraman, A., Li, Z., Smith, S. E., Unruh, J., Chen, S., Ward, C., Qian, P., Perry, J. M., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2018.12.093 · Cited by 146
Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion
Isern, J., Martín-Antonio, B., Ghazanfari, R., Martín, A. M., López, J. A., del Toro, R., Sánchez-Aguilera, A., Arranz, L., Martín-Pérez, D., Suárez-Lledó, M., et al. (2013) · Cell Reports · doi:10.1016/j.celrep.2013.03.041 · Cited by 141
Yarandi, S. S., Kulkarni, S., Saha, M., Sylvia, K. E., Sears, C. L., & Pasricha, P. J. (2020) · Gastroenterology · doi:10.1053/j.gastro.2020.03.050 · Cited by 138
Severe, N., Karabacak, N. M., Gustafsson, K., Baryawno, N., Courties, G., Kfoury, Y., Kokkaliaris, K. D., Rhee, C., Lee, D., Scadden, E. W., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.06.003 · Cited by 130
Abolition of aberrant neurogenesis ameliorates cognitive impairment after stroke in mice
Cuartero, M. I., de la Parra, J., Pérez-Ruiz, A., Bravo-Ferrer, I., Durán-Laforet, V., García-Culebras, A., García-Segura, J. M., Dhaliwal, J., Frankland, P. W., Lizasoain, I., et al. (2019) · Journal of Clinical Investigation · doi:10.1172/jci120412 · Cited by 128
Chemokine CXCL12 in neurodegenerative diseases: an SOS signal for stem cell-based repair
Li, M., Hale, J. S., Rich, J. N., Ransohoff, R. M., & Lathia, J. D. (2012) · Trends in Neurosciences · doi:10.1016/j.tins.2012.06.003 · Cited by 104
Gebara, E., Sultan, S., Kocher-Braissant, J., & Toni, N. (2013) · Frontiers in Neuroscience · doi:10.3389/fnins.2013.00145 · Cited by 102
Radial glial cells in the adult dentate gyrus: what are they and where do they come from?
Berg, D. A., Bond, A. M., Ming, G.-L., & Song, H. (2018) · F1000Research · doi:10.12688/f1000research.12684.1 · Cited by 89
Roles of HAUSP-mediated p53 regulation in central nervous system development
Kon, N., Zhong, J., Kobayashi, Y., Li, M., Szabolcs, M., Ludwig, T., Canoll, P. D., & Gu, W. (2011) · Cell Death & Differentiation · doi:10.1038/cdd.2011.12 · Cited by 79
Shefer, G., Rauner, G., Stuelsatz, P., Benayahu, D., & Yablonka‐Reuveni, Z. (2013) · The FEBS Journal · doi:10.1111/febs.12228 · Cited by 72
A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise
Gadomski, S., Fielding, C., García-García, A., Korn, C., Kapeni, C., Ashraf, S., Villadiego, J., Toro, R. D., Domingues, O., Skepper, J. N., et al. (2022) · Cell Stem Cell · doi:10.1016/j.stem.2022.02.008 · Cited by 65
Phenotypical and functional heterogeneity of neural stem cells in the aged hippocampus
Martín‐Suárez, S., Valero, J., Muro‐García, T., & Encinas, J. M. (2019) · Aging Cell · doi:10.1111/acel.12958 · Cited by 58
Popovitchenko, T., Park, Y., Page, N. F., Luo, X., Krsnik, Z., Liu, Y., Salamon, I., Stephenson, J. D., Kraushar, M. L., Volk, N. L., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-15412-8 · Cited by 55
Yoneda, Y., Kuramoto, N., & Kawada, K. (2019) · Neurochemistry International · doi:10.1016/j.neuint.2019.104505 · Cited by 39
Wang, X., Seekaew, P., Gao, X., & Chen, J. (2016) · eneuro · doi:10.1523/eneuro.0162-16.2016 · Cited by 31
Wen, P. H., De Gasperi, R., Sosa, M. A. G., Rocher, A. B., Friedrich, V. L., Hof, P. R., & Elder, G. A. (2005) · Development · doi:10.1242/dev.01946 · Cited by 30
Mendes-Jorge, L., Llombart, C., Ramos, D., López-Luppo, M., Valença, A., Nacher, V., Navarro, M., Carretero, A., Méndez-Ferrer, S., Rodriguez-Baeza, A., et al. (2012) · Experimental Eye Research · doi:10.1016/j.exer.2012.03.010 · Cited by 30
An L-Glutamine transporter isoform for neurogenesis facilitated by L-Theanine
Yoneda, Y. (2017) · Neurochemical Research · doi:10.1007/s11064-017-2317-6 · Cited by 23
Antidepressants and Cdk inhibitors: Releasing the brake on neurogenesis?
Chesnokova, V., & Pechnick, R. N. (2008) · Cell Cycle · doi:10.4161/cc.6446 · Cited by 19
The role of Atg7-mediated autophagy in ionizing radiation-induced neural stem cell damage
Shi, W., Liu, W., Ma, J., Lu, J., Yang, X., Wang, J., Cao, J., Tian, Y., Yang, H., & Zhang, L. (2020) · Gene · doi:10.1016/j.gene.2020.144485 · Cited by 19
Selective upregulation by theanine of Slc38a1 expression in neural stem cell for brain wellness
Yoneda, Y., Kawada, K., & Kuramoto, N. (2020) · Molecules · doi:10.3390/molecules25020347 · Cited by 13
“Since NSCs are shown to be enriched in particular restricted regions such as the subventricular zone (SVZ) and the subgranular zone (SGZ) of the hippocampal dentate gyrus in adult rodent brains [25], we have isolated the hippocampus from young adult mice with the overexpression of green fluorescent protein (GFP) in ce…”
Background
Metadata
- Authors
- Mignone, J. L., Kukekov, V., Chiang, A., Steindler, D., & Enikolopov, G.
- Venue
- Journal of Comparative Neurology
- Publisher
- Wiley
- Published
- 2004-01-12
- Volume
- 469
- Issue
- 3
- Pages
- 311-324
- ISSN
- 0021-9967 (ISSN-L)
- 1096-9861
- OpenAlex
- W2044110964
- Cited by
- 704
- References
- 5
- FWCI
- 7.30
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
