The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component
Open accessAbstract
Recently, macrophages have been characterized as having an M1 or M2 phenotype based on receptor expression, cytokine and effector molecule production, and function. The effects of macrophage phenotype upon tissue remodeling following the implantation of a biomaterial are largely unknown. The objectives of this study were to determine the effects of a cellular component within an implanted extracellular matrix (ECM) scaffold upon macrophage phenotype, and to determine the relationship between macrophage phenotype and tissue remodeling. Partial-thickness defects in the abdominal wall musculature of Sprague-Dawley rats were repaired with autologous body wall tissue, acellular allogeneic rat body wall ECM, xenogeneic pig urinary bladder tissue, or acellular xenogeneic pig urinary bladder ECM. At 3, 7, 14, and 28 days the host tissue response was characterized using histologic, immunohistochemical, and RT-PCR methods. The acellular test articles were shown to elicit a predominantly M2 type response and resulted in constructive remodeling, while those containing a cellular component, even an autologous cellular component, elicited a predominantly M1 type response and resulted in deposition of dense connective tissue and/or scarring. We conclude that the presence of cellular material within an ECM scaffold modulates the phenotype of the macrophages participating in the host response following implantation, and that the phenotype of the macrophages participating in the host response appears to be related to tissue remodeling outcome.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“What is more, it is widely accepted that during the degradation of implanted ECM bioscaffolds, ECM components are released into the environment and promote the transition to a remodeling phase [18,44,74,78].”
From Immune Response to Extracellular Matrix Bioscaffolds: A Comprehensive Review (2025) · cites this paper as [74]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Mechanistically, inadequate decellularization retains high amounts of damage-associated molecular patterns (DAMPs) or endotoxin, and excessive crosslinking biases macrophages toward pro-inflammatory phenotypes that drive fibrosis and poor remodeling [73,74].”
From Immune Response to Extracellular Matrix Bioscaffolds: A Comprehensive Review (2025) · cites this paper as [74]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Immunohistochemical and gene expression analysis using CCR7 and iNOS for M1 and CD163 and Arg for M2 macrophages revealed that cellular scaffolds had a majority of M1 phenotype and provided a dense, poorly organized collagenous FBC, while decellularized scaffolds favored the M2 phenotype, and displayed both neomatrix formation and early angiogenesis.”
From Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes (2024) · cites this paper as [180]
Experimental — accuracy not yet measuredIn the citing paper
“Recognition of the predominant MΦ phenotypic profile can provide a tool by which a tissue regenerative outcome can be predicted and possibly promoted [4].”
From Macrophage functional polarization (M1/M2) in response to varying fiber and pore dimensions of electrospun scaffolds (2013) · cites this paper as [4]
In the citing paper
“Brown et al. [83], who demonstrated that the presence of even an autologous cellular component attracted M1 cells, which was not observed in cell free constructs.”
From Biomaterial based modulation of macrophage polarization: a review and suggested design principles (2015) · cites this paper as [83]
In the citing paper
“Briefly, scaffold materials composed of extracellular matrix (ECM) have been shown to promote a switch from a predominantly M1 cell population immediately following implantation to a population enriched in M2 cells by 7–14 days post implantation [154–156].”
From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [156]
In the citing paper
“This constructive remodeling response has been directly linked to the ability of the material to modulate macrophage phenotype [154–157].”
From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [156]
In the citing paper
“Here we find that MΦ activation depends on biomaterial architecture in addition to previously implicated biomaterial chemistry and bioactivity (34–36).”
From Proangiogenic scaffolds as functional templates for cardiac tissue engineering (2010) · cites this paper as [34]
In the citing paper
“Previous in vivo studies demonstrated concurrent expression of M1 and M2 markers (37), and enhanced expression of M2 markers has been associated with improved outcomes for implanted biologic scaffolds (34).”
From Proangiogenic scaffolds as functional templates for cardiac tissue engineering (2010) · cites this paper as [34]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · cited 1×
“Relative expression of the M1 and M2 genes was calculated using the 2 ðDCt Þ method [23,24].”
MethodAnalyzing real-time PCR data by the comparative CT method
Schmittgen, T. D., & Livak, K. J. (2008) · Nature Protocols · doi:10.1038/nprot.2008.73 · Cited by 26,190 · cited 1×
“Relative expression of the M1 and M2 genes was calculated using the 2 ðDCt Þ method [23,24].”
MethodThe chemokine system in diverse forms of macrophage activation and polarization
MANTOVANI, A., SICA, A., SOZZANI, S., ALLAVENA, P., VECCHI, A., & LOCATI, M. (2004) · Trends in Immunology · doi:10.1016/j.it.2004.09.015 · Cited by 6,534 · cited 3×
“Multiple studies have shown that CCR7 is highly expressed on M1 polarized cells and that CD163 is highly indicative of M2 type antiinflammatory polarization [14,35,36].”
BackgroundMANTOVANI, A., SOZZANI, S., LOCATI, M., ALLAVENA, P., & SICA, A. (2002) · Trends in Immunology · doi:10.1016/s1471-4906(02)02302-5 · Cited by 5,528
Monocyte and macrophage heterogeneity
Gordon, S., & Taylor, P. R. (2005) · Nature Reviews Immunology · doi:10.1038/nri1733 · Cited by 5,190 · cited 1×
“M2 activated macrophages express IL-12, IL-23, and IL-10high; have high levels of scavenger, mannose, and galactose receptors; produce arginase in the place of arginine, subsequently producing ornithine and polyamines; are involved in polarized Th2 reactions; and possess the ability to facilitate tissue repair and reg…”
BackgroundMutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
M-1/M-2 Macrophages and the Th1/Th2 Paradigm
Mills, C. D., Kincaid, K., Alt, J. M., Heilman, M. J., & Hill, A. M. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.12.6166 · Cited by 3,287 · cited 2×
“Polarized macrophages are referred to as either M1 or M2 cells, mimicking the Th1/Th2 nomenclature [9].”
BackgroundMartínez, F. O., Gordon, S., Locati, M., & Mantovani, A. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.10.7303 · Cited by 2,399 · cited 1×
“Multiple studies have shown that CCR7 is highly expressed on M1 polarized cells and that CD163 is highly indicative of M2 type antiinflammatory polarization [14,35,36].”
BackgroundDecellularization of tissues and organs
Gilbert, T. W., Sellaro, T. L., & Badylak, S. F. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.014 · Cited by 2,222 · cited 2×
“The decellularization methods used to process ECM scaffolds may alter these molecules such that they can no longer negatively influence the host response or there may be a threshold amount required to alter the host response to an ECM scaffold [25].”
MethodSelf-citationThe many faces of macrophage activation
Mosser, D. M. (2003) · Journal of Leukocyte Biology · doi:10.1189/jlb.0602325 · Cited by 1,902 · cited 1×
“M2 activated macrophages express IL-12, IL-23, and IL-10high; have high levels of scavenger, mannose, and galactose receptors; produce arginase in the place of arginine, subsequently producing ornithine and polyamines; are involved in polarized Th2 reactions; and possess the ability to facilitate tissue repair and reg…”
Background
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–200 of 896
ECM hydrogel for the treatment of stroke: Characterization of the host cell infiltrate
Ghuman, H., Massensini, A. R., Donnelly, J., Kim, S., Medberry, C. J., Badylak, S. F., & Modo, M. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.03.014 · Cited by 142
“An M2-like phenotype polarization of invading macrophages has indeed been linked with peripheral nerve repair [54] and the constructive remodeling response of injected ECM [55].”
Self-citationExtracellular Matrix Bioscaffolds as Immunomodulatory Biomaterials
Dziki, J. L., Huleihel, L., Scarritt, M. E., & Badylak, S. F. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0538 · Cited by 141
“The thoroughness of decellularization of the source tissue is a critical determinant of the ability of these materials to elicit an anti-inflammatory macrophage/T cell (M2-like/Th2-like) host response.(62,63) In addition, the use of chemical cross-linking,(8,23) the anatomic origin of the source tissue from which the…”
Self-citationHydrated xenogeneic decellularized tracheal matrix as a scaffold for tracheal reconstruction
Remlinger, N. T., Czajka, C. A., Juhas, M. E., Vorp, D. A., Stolz, D. B., Badylak, S. F., Gilbert, S., & Gilbert, T. W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.01.067 · Cited by 141
Self-citationZhang, Y., Sime, W., Juhas, M., & Sjölander, A. (2013) · European Journal of Cancer · doi:10.1016/j.ejca.2013.06.005 · Cited by 146
Sicari, B. M., Johnson, S. A., Siu, B. F., Crapo, P. M., Daly, K. A., Jiang, H., Medberry, C. J., Tottey, S., Turner, N. J., & Badylak, S. F. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.04.017 · Cited by 140
Self-citationTissue Specificity of Decellularized Rhesus Monkey Kidney and Lung Scaffolds
Nakayama, K. H., Lee, C. C. I., Batchelder, C. A., & Tarantal, A. F. (2013) · PLoS ONE · doi:10.1371/journal.pone.0064134 · Cited by 139
Collagen Membrane and Immune Response in Guided Bone Regeneration: Recent Progress and Perspectives
Chu, C., Deng, J., Sun, X., Qu, Y., & Man, Y. (2017) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2016.0463 · Cited by 140
“6 13–15 M1 cells are thought to secrete proinflammatory factors that orchestrate the degradation of such degradable materials.”
Harnessing macrophage plasticity for tissue regeneration
Smith, T. D., Nagalla, R. R., Chen, E. Y., & Liu, W. F. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.04.012 · Cited by 138
“pared to autologous cellular material [28].”
The effect of source animal age upon extracellular matrix scaffold properties
Tottey, S., Johnson, S. A., Crapo, P. M., Reing, J. E., Zhang, L., Jiang, H., Medberry, C. J., Reines, B., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2010.09.006 · Cited by 138
Self-citationTherapeutically relevant aspects in bone repair and regeneration
Pérez, R. A., Seo, S.-J., Won, J., Lee, E., Jang, J.-H., Knowles, J. C., & Kim, H. (2015) · Materials Today · doi:10.1016/j.mattod.2015.06.011 · Cited by 136
The role of skin substitutes in the management of chronic cutaneous wounds
Greaves, N. S., Iqbal, S. A., Baguneid, M. S., & Bayat, A. (2013) · Wound Repair and Regeneration · doi:10.1111/wrr.12029 · Cited by 135
“Two macrophage phenotypes (M1 and M2) have previously been shown.(164,165) Both are essential at different phases of wound healing.”
BackgroundMethodInfluentialBiocompatibility Evolves: Phenomenology to Toxicology to Regeneration
Crawford, L., Wyatt, M., Bryers, J. D., & Ratner, B. D. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202002153 · Cited by 141
Costa, A., Naranjo, J. D., Londono, R., & Badylak, S. F. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a025676 · Cited by 135
“A positive correlation between the local tissue ratio of M2-like macrophages to M1-like macrophages (M2:M1) and constructive, functional outcomes has been shown for a large number of commercially available bioscaffold mesh devices (Brown et al. 2009).”
BackgroundInfluentialSelf-citationRegulation of decellularized matrix mediated immune response
Chakraborty, J., Roy, S., & Ghosh, S. (2020) · Biomaterials Science · doi:10.1039/c9bm01780a · Cited by 130
Shared signaling systems in myeloid cell-mediated muscle regeneration
Tidball, J. G., Dorshkind, K. A., & Wehling‐Henricks, M. (2014) · Development · doi:10.1242/dev.098285 · Cited by 151
“Indeed, the success of implantation of a biological scaffold to replace skeletal muscle was strongly influenced by whether the inflammatory response to the implant was biased toward M1 or M2 macrophages (Brown et al., 2009).”
BackgroundPeloso, A., Urbani, L., Cravedi, P., Katari, R., Maghsoudlou, P., Fallas, M. E. A., Sordi, V., Citro, A., Purroy, C., Niu, G., et al. (2016) · Annals of Surgery · doi:10.1097/sla.0000000000001364 · Cited by 133
Tran, T.-H., Rastogi, R., Shelke, J. D., & Amiji, M. M. (2015) · Scientific Reports · doi:10.1038/srep16632 · Cited by 138
Kawecki, M., Łabuś, W., Klama‐Baryła, A., Kitala, D., Kraut, M., Glik, J., Misiuga, M., Nowak, M., Bielecki, T., & Kasperczyk, A. (2017) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33865 · Cited by 129
Sadr, N., Pippenger, B. E., Scherberich, A., Wendt, D. J., Mantero, S., Martín, I., & Papadimitropoulos, A. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.03.082 · Cited by 127
Scaffold strategies for modulating immune microenvironment during bone regeneration
He, J., Chen, G., Liu, M., Xu, Z., Chen, H., Yang, L., & Lv, Y. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.110411 · Cited by 128
Matrix-bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype
Huleihel, L., Bartolacci, J. G., Dziki, J. L., Vorobyov, T., Arnold, B., Scarritt, M. E., Pineda Molina, C., LoPresti, S. T., Brown, B. N., Naranjo, J. D., et al. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2017.0102 · Cited by 116
Self-citationKeane, T. J., & Badylak, S. F. (2015) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1874 · Cited by 139
Self-citationRecent advances in decellularized biomaterials for wound healing
Xiao, H., Chen, X., Liu, X., Wen, G., & Yu, Y. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100589 · Cited by 124
Zaidi, A. H., Nathan, M., Emani, S. M., Baird, C. W., del Nido, P. J., Gauvreau, K., Harris, M. H., Sanders, S. P., & Padera, R. F. (2014) · Journal of Thoracic and Cardiovascular Surgery · doi:10.1016/j.jtcvs.2014.02.081 · Cited by 117
“75 Patch 2 (33) 2 (20) 2 (25) 1 (25) Free edge 4 (67) 8 (80) 4 (50) 3 (75) Both 0 (0) 0 (0) 2 (25) 0 (0)”
BackgroundGarg, K., Ward, C., Rathbone, C., & Corona, B. T. (2014) · Cell and Tissue Research · doi:10.1007/s00441-014-2006-6 · Cited by 109
“…engineering applications (Keane and Badylak 2014) and have been studied in preclinical skeletal muscle VML injury models frequently over the last decade (e.g., Brown et al. 2009; Corona et al. 2013a, 2013b; Gamba et al. 2002; Machingal et al. 2011; Merritt et al. 2010a; Valentin et al. 2010).”
Cerqueira, S. R., Lee, Y., Cornelison, R., Mertz, M. W., Wachs, R. A., Schmidt, C. E., & Bunge, M. B. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.05.049 · Cited by 117
“Previous reports have shown that acellular scaffolds, including iPN, promote immune cell bias toward M2 regenerative phenotypes [19, 52].”
Patel, D. K., Dutta, S. D., Hexiu, J., Ganguly, K., & Lim, K. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2021.119077 · Cited by 117
Boehler, R. M., Kuo, R., Shin, S., Goodman, A. G., Pilecki, M. A., Leonard, J. N., & Shea, L. D. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.25175 · Cited by 117
“An emerging concept in biomaterial-based applications involves targeting macrophages, through surface properties or gene/drug delivery, as a means to promote a regenerative response by macrophages (Boehler et al., 2011; Brown et al., 2009; Mokarram et al., 2012).”
BackgroundImmunogenicity of Decellularized Porcine Liver for Bioengineered Hepatic Tissue
Mirmalek-Sani, S.-H., Sullivan, D. C., Zimmerman, C., Shupe, T. D., & Petersen, B. E. (2013) · The American Journal of Pathology · doi:10.1016/j.ajpath.2013.05.002 · Cited by 115
Wagner, D. E., Bonvillain, R. W., Jensen, T., Girard, E. D., Bunnell, B. A., Finck, C. M., Hoffman, A. M., & Weiss, D. J. (2013) · Respirology · doi:10.1111/resp.12102 · Cited by 120
Al‐Maawi, S., Orłowska, A., Sader, R., Kirkpatrick, C. J., & Ghanaati, S. (2017) · Seminars in Immunology · doi:10.1016/j.smim.2017.06.001 · Cited by 123
Small Intestinal Submucosa Segments as Matrix for Tissue Engineering: Review
Andrée, B., Bär, A., Haverich, A., & Hilfiker, A. (2012) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2012.0583 · Cited by 114
Biologic Scaffold Remodeling in a Dog Model of Complex Musculoskeletal Injury
Turner, N. J., Badylak, J. S., Weber, D. J., & Badylak, S. F. (2011) · Journal of Surgical Research · doi:10.1016/j.jss.2011.11.1029 · Cited by 113
Self-citationWong, M. L., Wong, J. L., Vapniarsky, N., & Griffiths, L. G. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.03.024 · Cited by 112
Titanium surfaces characteristics modulate macrophage polarization
Zhang, Y., Cheng, X., Jansen, J. A., Yang, F., & van den Beucken, J. J. J. P. (2019) · Materials Science and Engineering: C · doi:10.1016/j.msec.2018.10.065 · Cited by 111
Zhang, Q., Johnson, J. A., Dunne, L. W., Chen, Y., Iyyanki, T. S., Wu, Y., Chang, E. I.-F., Branch-Brooks, C. D., Robb, G. L., & Butler, C. E. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.02.017 · Cited by 111
Calcium silicate bioactive ceramics induce osteogenesis through oncostatin M
Zhou, P., Xia, D., Ni, Z., Ou, T., Wang, Y., Zhang, H., Mao, L., Lin, K., Xu, S., & Liu, J. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.09.018 · Cited by 109
“Classically, inflammatory macrophages (M1), with the typical surface marker CD11c, are well known to enhance Th1-biased inflammation by pro-inflammatory cytokines (IL-6, TNF-α), which enhance osteoclastic activities [23].”
BackgroundIn vitro response of macrophage polarization to a keratin biomaterial
Fearing, B. V., & Dyke, M. E. V. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.04.003 · Cited by 111
Faulk, D. M., Wildemann, J. D., & Badylak, S. F. (2015) · Journal of Clinical and Experimental Hepatology · doi:10.1016/j.jceh.2014.03.043 · Cited by 108
Self-citationBöer, U., Lohrenz, A., Klingenberg, M., Pich, A., Haverich, A., & Wilhelmi, M. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.09.015 · Cited by 107
Kc, P., Hong, Y., & Zhang, G. (2019) · Regenerative Biomaterials · doi:10.1093/rb/rbz017 · Cited by 107
Porcine Lung-Derived Extracellular Matrix Hydrogel Properties Are Dependent on Pepsin Digestion Time
Pouliot, R. A., Young, B. M., Link, P. A., Park, H. E., Kahn, A. R., Shankar, K., Schneck, M. B., Weiss, D. J., & Heise, R. L. (2020) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2020.0042 · Cited by 108
Silk sericin as a biomaterial for tissue engineering: a review
Arango, M. C., Montoya, Y., Peresin, M. S., Bustamante, J., & Álvarez‐López, C. (2020) · International Journal of Polymeric Materials · doi:10.1080/00914037.2020.1785454 · Cited by 106
“Thus, the structure must have the capacity to biodegrade at the time of promoting cells to produce their own extracellular matrix([40]).”
BackgroundGoonoo, N., Bhaw‐Luximon, A., Bowlin, G. L., & Jhurry, D. (2013) · Polymer International · doi:10.1002/pi.4474 · Cited by 107
Everts, P. A., Lana, J. F., Onishi, K., Buford, D., Peng, J. W., Mahmood, A., da Fonseca, L. F., van Zundert, A. A. J., & Podesta, L. (2023) · Biomedicines · doi:10.3390/biomedicines11071922 · Cited by 112
Automated Decellularization of Intact, Human-Sized Lungs for Tissue Engineering
Price, A., Godin, L. M., Domek, A., Cotter, T., D’Cunha, J., Taylor, D. A., & Panoskaltsis‐Mortari, A. (2014) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2013.0756 · Cited by 105
“Yet others have found that some forms of inflammation into decellularized tissue scaffolds may be beneficial for appropriate tissue remodeling.(27) A major challenge with preparation of decellularized organs is to achieve consistency of the final product in terms of composition, sterility, and mechanical properties.”
BackgroundLi, Y., Meng, H., Liu, Y., Narkar, A. R., & Lee, B. P. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b01364 · Cited by 103
“[77].”
Topography-mediated immunomodulation in osseointegration; Ally or Enemy
Shirazi, S., Ravindran, S., & Cooper, L. F. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121903 · Cited by 105
Essential Biomaterials Science
Williams, D. F. (2014) · Cambridge University Press eBooks · doi:10.1017/cbo9781139026086 · Cited by 103
From In Vitro to In Situ Tissue Engineering
Sengupta, D., Waldman, S. D., & Li, S. (2014) · Annals of Biomedical Engineering · doi:10.1007/s10439-014-1022-8 · Cited by 102
Targeting Macrophage Subsets for Infarct Repair
Ben-Mordechai, T., Palevski, D., Glucksam, Y., Elron-Gross, I., Margalit, R., & Leor, J. (2014) · Journal of Cardiovascular Pharmacology and Therapeutics · doi:10.1177/1074248414534916 · Cited by 100
Keeping an Eye on Decellularized Corneas: A Review of Methods, Characterization and Applications
Wilson, S., Sidney, L., Dunphy, S., Rose, J., & Hopkinson, A. (2013) · Journal of Functional Biomaterials · doi:10.3390/jfb4030114 · Cited by 100
“Incomplete removal of cellular material can lead to adverse host response, and cytocompatibility issues associated with the DC [104–106].”
BackgroundAgrawal, V., Brown, B. N., Beattie, A. J., Gilbert, T. W., & Badylak, S. F. (2009) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.200 · Cited by 99
Self-citationJiang, K., Chaimov, D., Patel, S. N., Liang, J., Wiggins, S., Samojlik, M. M., Rubiano, A. M., Simmons, C. S., & Stabler, C. L. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.08.057 · Cited by 102
“To optimize this balance for this study, a combination of published decellularization approaches, including osmotic burst, freeze and thaw cycles, mechanical disruption, and moderate enzymatic treatment, were strategically employed [26, 34-36].”
A Preliminary Study on the Potential of Manuka Honey and Platelet-Rich Plasma in Wound Healing
Sell, S. A., Wolfe, P. S., Spence, A. J., Rodriguez, I. A., McCool, J. M., Petrella, R. L., Garg, K., Ericksen, J. J., & Bowlin, G. L. (2012) · International Journal of Biomaterials · doi:10.1155/2012/313781 · Cited by 98
Xu, K., Cantu, D. A., Fu, Y., Kim, J., Zheng, X., Hematti, P., & Kao, W. J. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.06.021 · Cited by 99
Urciuolo, A., Urbani, L., Perin, S., Maghsoudlou, P., Scottoni, F., Gjinovci, A., Collins‐Hooper, H., Loukogeorgakis, S., Tyraskis, A., Torelli, S., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-26371-y · Cited by 106
“The degradation of the ECM-derived scaffolds has shown to have a positive influence on tissue regeneration since it induces the release of bioactive molecules that modulate macrophage polarization phenotype and activate local stem cells 49–51 .”
Du, L., Tang, H., Ma, Z.-F., Xu, J., Gao, W., Chen, J., Gan, W., Zhang, Z., Xin-bing, Y., Zhou, X., et al. (2011) · Digestive Diseases and Sciences · doi:10.1007/s10620-011-1689-8 · Cited by 98
“The former are activated by Th1 cytokines interleukin (IL)-12 and IFN-c, releasing proinflammatory cytokines to enhance immune responses and injured host tissues; whereas the latter are activated by Th2 cytokines IL-4 and IL-13, releasing IL-10 and TGFb1 to suppress immune responses and restore the injured tissues [ 9…”
BackgroundBone Regeneration from PLGA Micro-Nanoparticles
Ortega-Oller, I., Padial‐Molina, M., Galindo‐Moreno, P., O’Valle, F., Jódar‐Reyes, A. B., & Peula-García, J. M. (2015) · BioMed Research International · doi:10.1155/2015/415289 · Cited by 97
“A controlled initial burst followed by a sustained release significantly improves the in vivo bone regeneration [117–119].”
BackgroundChallenges to acellular biological scaffold mediated skeletal muscle tissue regeneration
Corona, B. T., & Greising, S. M. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.07.020 · Cited by 94
Kočí, Z., Výborný, K., Dubišová, J., Vacková, I., Jäger, A., Lunov, O., Jiráková, K., & Kubinová, Š. (2017) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2017.0089 · Cited by 96
Immunoengineering nerve repair
Mokarram, N., Dymanus, K., Srinivasan, A., Lyon, J. G., Tipton, J., Chu, J. K., ENGLISH, A. W., & Bellamkonda, R. V. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1705757114 · Cited by 97
Seebach, E., Freischmidt, H., Holschbach, J., Fellenberg, J., & Richter, W. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.07.017 · Cited by 92
Biomimetic Approaches for Bone Tissue Engineering
Ng, J., Spiller, K. L., Bernhard, J., & Vunjak‐Novakovic, G. (2016) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2016.0289 · Cited by 100
Zhang, X., Wu, J., Ye, B., Wang, Q., Xie, X., & Shen, H. (2016) · BMC Complementary and Alternative Medicine · doi:10.1186/s12906-016-1273-z · Cited by 93
Suamte, L., Tirkey, A., & Babu, P. J. (2022) · Smart Materials in Medicine · doi:10.1016/j.smaim.2022.09.005 · Cited by 96
Striated muscle function, regeneration, and repair
Shadrin, I. Y., Khodabukus, A., & Bursac, N. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-016-2285-z · Cited by 106
“Cellular debris in decellularized scaffolds [165, 166] and excessive modification of native ECM structure with cross-linkers [167] are both associated with poor remodeling outcomes.”
BackgroundWang, R. M., Johnson, T. D., He, J., Rong, Z., Wong, M., Nigam, V., Behfar, A., Xu, Y., & Christman, K. L. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.03.016 · Cited by 90
“Similarly, polarized macrophages were assessed towards the pro-remodeling M2 or pro-inflammatory M1 phenotype [27,49].”
BackgroundMethodKoržinskas, T., Jung, O., Smeets, R., Stojanović, S., Najman, S. J., Glenske, K., Hahn, M. P., Wenisch, S., Schnettler, R., & Barbeck, M. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19102952 · Cited by 91
“Based on their molecule expression, macrophages are more or less divided into pro-inflammatory M1- and anti-inflammatory M2 subtypes [19,20].”
BackgroundGuillory, R. J., Bowen, P. K., Hopkins, S. P., Shearier, E. R., Earley, E. J., Gillette, A. A., Aghion, E., Bocks, M. L., Drelich, J. W., & Goldman, J. (2016) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00591 · Cited by 91
Daly, K. A., Liu, S., Agrawal, V., Brown, B. N., Johnson, S. A., Medberry, C. J., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.09.040 · Cited by 91
Self-citationDecellularization of human stromal refractive lenticules for corneal tissue engineering
Yam, G. H., Yusoff, N. Z. B. M., Goh, T.-W., Setiawan, M., Lee, X.-W., Liu, Y., & Mehta, J. S. (2016) · Scientific Reports · doi:10.1038/srep26339 · Cited by 91
Meng, F. W., Slivka, P. F., Dearth, C. L., & Badylak, S. F. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2014.12.044 · Cited by 87
Self-citationThird generation poly(hydroxyacid) composite scaffolds for tissue engineering
Goonoo, N., Bhaw‐Luximon, A., Passanha, P., Esteves, S., & Jhurry, D. (2016) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33674 · Cited by 89
The Biocompatibility of Implant Materials
Ratner, B. D. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-800196-7.00003-7 · Cited by 88
Antmen, E., Vrana, N. E., & Hasırcı, V. (2021) · Biomaterials Science · doi:10.1039/d1bm00840d · Cited by 90
Sicari, B. M., Dearth, C. L., & Badylak, S. F. (2013) · The Anatomical Record · doi:10.1002/ar.22794 · Cited by 88
“…et al., 2004; Brennan et al., 2006, 2008; Gilbert et al., 2007a,b; Beattie et al., 2009; Reing et al., 2009; Agrawal et al., 2012), and (4) modulation of the host innate immune response toward a regulatory and constructive phenotype (Allman et al., 2001; Badylak et al., 2001; Brown et al., 2009).”
BackgroundSelf-citationInduction of specific macrophage subtypes by defined micro-patterned structures
Bartneck, M., Schulte, V. A., Paul, N. E., Diez, M., Lensen, M. C., & Zwadlo-Klarwasser, G. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.04.025 · Cited by 87
Yeon, B., Park, M. H., Moon, H. J., Kim, S.-J., Cheon, Y. W., & Jeong, B. (2013) · Biomacromolecules · doi:10.1021/bm400868j · Cited by 85
Airway tissue engineering: an update
Fishman, J. M., Wiles, K., Lowdell, M. W., Coppi, P. D., Elliott, M. J., Atala, A., & Birchall, M. (2014) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2014.938631 · Cited by 85
“Regarding the first criterion, the urinary bladder [66] and small intestine submucosa in pigs [67] have aided in the de novo formation of large amounts of skeletal muscle.”
BackgroundZhu, Y., Matsumura, Y., & Wagner, W. R. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.02.032 · Cited by 85
Injectable Biomimetic Hydrogels as Tools for Efficient T Cell Expansion and Delivery
Weiden, J., Voerman, D., Dölen, Y., Das, R. K., van Duffelen, A., Hammink, R., Eggermont, L. J., Rowan, A. E., Tel, J., & Figdor, C. G. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02798 · Cited by 84
Xu, M., Su, T., Jin, X., Li, Y., Yao, Y., Liu, K., Chen, K., Lu, F., & He, Y. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.08.015 · Cited by 86
Goonoo, N., Khanbabaee, B., Steuber, M., Bhaw‐Luximon, A., Jonas, U., Pietsch, U., Jhurry, D., & Schönherr, H. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.7b00150 · Cited by 83
Amini, Z., & Lari, R. (2021) · Tissue and Cell · doi:10.1016/j.tice.2021.101494 · Cited by 90
Decellularized periosteum as a potential biologic scaffold for bone tissue engineering
Chen, K., Lin, X., Zhang, Q., Ni, J., Li, J., Xiao, J. R., Wang, Y., Ye, Y., Chen, L., Jin, K., et al. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.02.020 · Cited by 83
Gothard, D., Smith, E. L., Kanczler, J. M., Black, C. R. M., Wells, J. A., Roberts, C., White, L. J., Qutachi, O., Peto, H., Rashidi, H., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0145080 · Cited by 82
Macrophage phenotypes in the collagen-induced foreign body reaction in rats
van Putten, S. M., Ploeger, D. T. A., Popa, E. R., & Bank, R. A. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.01.022 · Cited by 82
Dearth, C. L., Keane, T. J., Carruthers, C. A., Reing, J. E., Huleihel, L., Ranallo, C. A., Kollar, E. W., & Badylak, S. F. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.01.038 · Cited by 82
Self-citationApplications of electrospun scaffolds with enlarged pores in tissue engineering
Zhang, Y., Zhang, M., Cheng, D., Xu, S., Du, C., Xie, L., & Zhao, W. (2022) · Biomaterials Science · doi:10.1039/d1bm01651b · Cited by 83
Decellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies
Chan, L. K. Y., Leung, V., Tam, V., Lu, W. W., Sze, K. Y., & Cheung, K. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2012.09.005 · Cited by 84
An Autologous Muscle Tissue Expansion Approach for the Treatment of Volumetric Muscle Loss
Ward, C. L., Ji, L. L., & Corona, B. T. (2015) · BioResearch open access · doi:10.1089/biores.2015.0009 · Cited by 81
Stapleton, T. W., Ingram, J., Fisher, J., & Ingham, E. (2010) · Tissue Engineering. Part A · doi:10.1089/ten.tea.2009.0807 · Cited by 88
Liang, K., Zhao, C., Song, C., Zhao, L., Qiu, P., Wang, S., Zhu, J., Gong, Z., Liu, Z., Tang, R., et al. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c16217 · Cited by 82
Ghanaati, S., Barbeck, M., Booms, P., Lorenz, J., Kirkpatrick, C. J., & Sader, R. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.04.017 · Cited by 80
“cells or cell remnants), and such remnants were previously identified as inducing factors of pro-inflammatory responses against those kinds of biomaterials [17–20].”
BackgroundToward Regeneration of the Heart: Bioengineering Strategies for Immunomodulation
Ferrini, A., Stevens, M. M., Sattler, S., & Rosenthal, N. A. (2019) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2019.00026 · Cited by 79
“The effect of biomaterials on macrophage polarization has been investigated by several groups, focusing on the responses to cellular or acellular grafts (65), natural (66) or synthetic (67) origin and different fibers and porous sizes (68, 69).”
BackgroundInjectable hydrogels of optimized acellular nerve for injection in the injured spinal cord
Cornelison, R. C., Gonzalez‐Rothi, E. J., Porvasnik, S., Wellman, S. M., Park, J., Fuller, D. D., & Schmidt, C. E. (2018) · Biomedical Materials · doi:10.1088/1748-605x/aaab82 · Cited by 79
“…to a biomaterial is a commonly used parameter for evaluating the response to implantation, as assessed by the ratio of pro-inflammatory M1 macrophages to anti-inflammatory M2 macrophages (Kelly et al., 2017; Wang et al., 2017; Sicari et al., 2014; Mokarram et al., 2012; Brown et al., 2009).”
BackgroundHost Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling
Fu, W. E., Liu, S., Chen, M., Tian, G., Zha, K., Yang, Z., Jiang, S., Li, M., Sui, X., Chen, Z., et al. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.664592 · Cited by 78
“As mentioned previously, DNA may be one cause of skewed polarization toward an M1 proinflammatory phenotype (Brown et al., 2009).”
BackgroundInfluentialMacrophages Modulate Engineered Human Tissues for Enhanced Vascularization and Healing
Spiller, K. L., Freytes, D. O., & Vunjak‐Novakovic, G. (2014) · Annals of Biomedical Engineering · doi:10.1007/s10439-014-1156-8 · Cited by 80
Bonito, V., Smits, A. I. P. M., Goor, O. J. G. M., Ippel, B. D., Driessen‐Mol, A., Münker, T. J. A. G., Bosman, A. W., Mes, T., Dankers, P. Y. W., & Bouten, C. V. C. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.02.032 · Cited by 80
Metadata
- Authors
- Brown, B. N., Valentin, J. E., Stewart-Akers, A. M., McCabe, G. P., & Badylak, S. F.
- Affiliations
- University of Pittsburgh (US)
- Purdue University West Lafayette (US)
- Corresponding author
- Badylak, Stephen F.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 30
- Issue
- 8
- Pages
- 1482-1491
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC2805023
- OpenAlex
- W1999579326
- Cited by
- 896
- References
- 38
- Citations by year
- 2026: 23 · 2025: 28 · 2024: 37 · 2023: 46 · 2022: 61 · 2021: 73 · 2020: 47 · 2019: 69 · 2018: 63 · 2017: 73 · 2016: 59 · 2015: 70 · 2014: 76 · 2013: 60 · 2012: 38
- FWCI
- 36.84
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (F31EB007914)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- macrophage phenotype, macrophage polarization, tissue remodeling, extracellular matrix scaffolds, acellular scaffolds, cellular scaffolds, host tissue response, scar formation, connective tissue deposition
- MeSH terms
- Animals, Cell Nucleus, Gene Expression Regulation, Immunohistochemistry, Indoles, Macrophages, Phenotype, Transplantation, Autologous, Transplantation, Heterologous, Transplantation, Homologous, Rats, Sprague-Dawley, Muscle, Skeletal, Prosthesis Implantation, Sus scrofa, Rats, Tissue Scaffolds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
