The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis
Open accessAbstract
SUMMARY Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into mature miRNAs. Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer cells. CD43 mediates the accumulation of Dicer specifically in cancer exosomes. Cancer exosomes mediate an efficient and rapid silencing of mRNAs to reprogram the target cell transcriptome. Exosomes derived from cells and sera of patients with breast cancer instigate non-tumorigenic epithelial cells to form tumors in a Dicer-dependent manner. These findings offer opportunities for the development of exosomes based biomarkers and therapies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
2 retracted
RETRACTED: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer
Taylor, D. D., & Gercel-Taylor, C. (2008) · Gynecologic Oncology · doi:10.1016/j.ygyno.2008.04.033 · Cited by 2,288
RetractedA TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function
Melo, S. A., Ropero, S., Moutinho, C., Aaltonen, L. A., Yamamoto, H., Calin, G. A., Rossi, S., Fernandez, A. F., Carneiro, F., Oliveira, C., et al. (2009) · Nature Genetics · doi:10.1038/ng.317 · Cited by 373
Self-citationRetractedMicroRNAs: Target Recognition and Regulatory Functions
Bartel, D. P. (2009) · Cell · doi:10.1016/j.cell.2009.01.002 · Cited by 20,068
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
The functions of animal microRNAs
Ambros, V. (2004) · Nature · doi:10.1038/nature02871 · Cited by 10,728
MicroRNA expression profiles classify human cancers
Lu, J., Getz, G., Miska, E. A., Alvarez-Saavedra, E., Lamb, J., Peck, D., Sweet-Cordero, A., Ebert, B. L., Mak, R. H., Ferrando, A. A., et al. (2005) · Nature · doi:10.1038/nature03702 · Cited by 9,539
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Exosomes: composition, biogenesis and function
Théry, C., Zitvogel, L., & Amigorena, S. (2002) · Nature Reviews Immunology · doi:10.1038/nri855 · Cited by 5,346
Skog, J., Würdinger, T., van Rijn, S., Meijer, D. H., Gainche, L., Curry, W. T., Carter, B. S., Krichevsky, A. M., & Breakefield, X. O. (2008) · Nature Cell Biology · doi:10.1038/ncb1800 · Cited by 5,001
Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET
Peinado, H., Alečković, M., Lavotshkin, S., Matei, I., Costa-Silva, B., Moreno-Bueno, G., Hergueta-Redondo, M., Williams, C., García-Santos, G., Ghajar, C. M., et al. (2012) · Nature Medicine · doi:10.1038/nm.2753 · Cited by 3,715
Non-coding RNAs and cancer: microRNAs and beyond
Raia, R. M., & Calin, G. A. (2011) · Journal of Nucleic Acids Investigation · doi:10.4081/jnai.2011.e5 · Cited by 3,382
Self-citationExportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
Yi, R., Qin, Y., Macara, I. G., & Cullen, B. R. (2003) · Genes & Development · doi:10.1101/gad.1158803 · Cited by 3,010
Tumour invasion and metastasis initiated by microRNA-10b in breast cancer
Ma, L., Teruya-Feldstein, J., & Weinberg, R. A. (2007) · Nature · doi:10.1038/nature06174 · Cited by 2,605
Exosomes – vesicular carriers for intercellular communication
Simons, M., & Raposo, G. (2009) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2009.03.007 · Cited by 2,351
TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
Chendrimada, T. P., Gregory, R. I., Kumaraswamy, E., Norman, J., Cooch, N., Nishikura, K., & Shiekhattar, R. (2005) · Nature · doi:10.1038/nature03868 · Cited by 2,100
Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells
Al-Nedawi, K., Meehan, B., Micallef, J., Lhotak, V., May, L., Guha, A., & Rak, J. (2008) · Nature Cell Biology · doi:10.1038/ncb1725 · Cited by 2,034
Shedding microvesicles: artefacts no more
Cocucci, E., Racchetti, G., & Meldolesi, J. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2008.11.003 · Cited by 1,742
Functional delivery of viral miRNAs via exosomes
Pegtel, D. M., Cosmopoulos, K., Thorley-Lawson, D. A., van Eijndhoven, M. A. J., Hopmans, E. S., Lindenberg, J. L., de Gruijl, T. D., Würdinger, T., & Middeldorp, J. M. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914843107 · Cited by 1,641
Human RISC Couples MicroRNA Biogenesis and Posttranscriptional Gene Silencing
Gregory, R. I., Chendrimada, T. P., Cooch, N., & Shiekhattar, R. (2005) · Cell · doi:10.1016/j.cell.2005.10.022 · Cited by 1,617
Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes.
Montecalvo, A., Larregina, A. T., Shufesky, W. J., Beer Stolz, D., Sullivan, M. L. G., Karlsson, J. M., Baty, C. J., Gibson, G. A., Erdos, G., Wang, Z., et al. (2012) · Blood · doi:10.1182/blood-2011-02-338004 · Cited by 1,429
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration
Luga, V., Zhang, L., Viloria-Petit, A. M., Ogunjimi, A. A., Inanlou, M. R., Chiu, E., Buchanan, M., Hosein, A. N., Basik, M., & Wrana, J. L. (2012) · Cell · doi:10.1016/j.cell.2012.11.024 · Cited by 1,311
Escola, J.-M., Kleijmeer, M. J., Stoorvogel, W., Griffith, J. M., Yoshie, O., & Geuze, H. J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.32.20121 · Cited by 1,228
Secreted Monocytic miR-150 Enhances Targeted Endothelial Cell Migration
Zhang, Y., Liu, D., Chen, X., Li, J., Li, L., Bian, Z., Sun, F., Lu, J., Yin, Y., Cai, X., et al. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.06.010 · Cited by 1,195
Exosomes: secreted vesicles and intercellular communications
Théry, C. (2011) · F1000 Biology Reports · doi:10.3410/b3-15 · Cited by 989
High Levels of Exosomes Expressing CD63 and Caveolin-1 in Plasma of Melanoma Patients
Logozzi, M., De Milito, A., Lugini, L., Borghi, M., Calabrò, L., Spada, M., Perdicchio, M., Marino, M. L., Federici, C., Iessi, E., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005219 · Cited by 960
Exosomes in tumor microenvironment influence cancer progression and metastasis
Kahlert, C., & Kalluri, R. (2013) · Journal of Molecular Medicine · doi:10.1007/s00109-013-1020-6 · Cited by 872
Self-citationProteomic profiling of exosomes: Current perspectives
Simpson, R. J., Jensen, S. S., & Lim, J. W. E. (2008) · PROTEOMICS · doi:10.1002/pmic.200800109 · Cited by 857
BM mesenchymal stromal cell–derived exosomes facilitate multiple myeloma progression
Roccaro, A. M., Sacco, A., Maiso, P., Azab, A. K., Tai, Y.-T., Reagan, M., Azab, F., Flores, L. M., Campigotto, F., Weller, E., et al. (2013) · Journal of Clinical Investigation · doi:10.1172/jci66517 · Cited by 794
Kosaka, N., Iguchi, H., Hagiwara, K., Yoshioka, Y., Takeshita, F., & Ochiya, T. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.446831 · Cited by 771
A MicroRNA Targeting Dicer for Metastasis Control
Martello, G., Rosato, A., Ferrari, F., Manfrin, A., Cordenonsi, M., Dupont, S., Enzo, E., Guzzardo, V., Rondina, M., Spruce, T., et al. (2010) · Cell · doi:10.1016/j.cell.2010.05.017 · Cited by 667
Haase, A. D., Jaskiewicz, L., Zhang, H., Lainé, S., Sack, R., Gatignol, A., & Filipowicz, W. (2005) · EMBO reports · doi:10.1038/sj.embor.7400509 · Cited by 657
Kogure, T., Lin, W.-L., Yan, I. K., Braconi, C., & Patel, T. (2011) · Hepatology · doi:10.1002/hep.24504 · Cited by 551
Macrophage microvesicles induce macrophage differentiation and miR-223 transfer
Ismail, N., Wang, Y., Dakhlallah, D., Moldovan, L., Agarwal, K., Batte, K., Shah, P., Wisler, J., Eubank, T. D., Tridandapani, S., et al. (2013) · Blood · doi:10.1182/blood-2011-08-374793 · Cited by 520
RNAi: The Nuts and Bolts of the RISC Machine
Filipowicz, W. (2005) · Cell · doi:10.1016/j.cell.2005.06.023 · Cited by 482
Pant, S., Hilton, H., & Burczynski, M. E. (2012) · Biochemical Pharmacology · doi:10.1016/j.bcp.2011.12.037 · Cited by 473
siRNA and miRNA: an insight into RISCs
Tang, G. (2005) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2004.12.007 · Cited by 449
In vitro reconstitution of the human RISC-loading complex
MacRae, I. J., Ma, E., Zhou, M., Robinson, C. V., & Doudna, J. A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0710869105 · Cited by 434
A human, ATP-independent, RISC assembly machine fueled by pre-miRNA
Maniataki, E., & Mourelatos, Z. (2005) · Genes & Development · doi:10.1101/gad.1384005 · Cited by 419
Visualization of Retroviral Replication in Living Cells Reveals Budding into Multivesicular Bodies
Sherer, N. M., Lehmann, M. J., Jimenez‐Soto, L. F., Ingmundson, A., Horner, S. M., Cicchetti, G., Allen, P. G., Pypaert, M., Cunningham, J. M., & Mothes, W. (2003) · Traffic · doi:10.1034/j.1600-0854.2003.00135.x · Cited by 410
EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2
Shen, J., Xia, W., Khotskaya, Y. B., Huo, L., Nakanishi, K., Lim, S.-O., Du, Y., Wang, Y., Chang, W.-C., Chen, C.-H., et al. (2013) · Nature · doi:10.1038/nature12080 · Cited by 356
A Genetic Defect in Exportin-5 Traps Precursor MicroRNAs in the Nucleus of Cancer Cells
Melo, S. A., Moutinho, C., Ropero, S., Calin, G. A., Rossi, S., Spizzo, R., Fernandez, A. F., Davalos, V., Villanueva, A., Montoya, G., et al. (2010) · Cancer Cell · doi:10.1016/j.ccr.2010.09.007 · Cited by 346
Self-citationYan, L. X., Wu, Q. N., Zhang, Y., Li, Y. Y., Liao, D. Z., Hou, J. H., Fu, J., Zeng, M. S., Yun, J. P., Wu, Q. L., et al. (2011) · Breast Cancer Research · doi:10.1186/bcr2803 · Cited by 323
Exosomes Derived from HIV-1-infected Cells Contain Trans-activation Response Element RNA
Narayanan, A., Iordanskiy, S., Das, R., Van Duyne, R., Santos, S., Jaworski, E., Guendel, I., Sampey, G., Dalby, E., Iglesias-Ussel, M., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.438895 · Cited by 316
Pten constrains centroacinar cell expansion and malignant transformation in the pancreas
Stanger, B. Z., Stiles, B., Lauwers, G. Y., Bardeesy, N., Mendoza, M., Wang, Y., Greenwood, A., Cheng, K.-H., McLaughlin, M., Brown, D., et al. (2005) · Cancer Cell · doi:10.1016/j.ccr.2005.07.015 · Cited by 294
Protein Targeting to Exosomes/Microvesicles by Plasma Membrane Anchors
Shen, B., Wu, N., Yang, J.-M., & Gould, S. J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.208660 · Cited by 285
Distinct Roles for Tsg101 and Hrs in Multivesicular Body Formation and Inward Vesiculation
Razi, M., & Futter, C. E. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-11-1054 · Cited by 255
Melo, S., Villanueva, A., Moutinho, C., Davalos, V., Spizzo, R., Ivan, C., Rossi, S., Setien, F., Casanovas, O., Simo-Riudalbas, L., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1014720108 · Cited by 253
Self-citationO’Brien, K., Rani, S., Corcoran, C., Wallace, R., Hughes, L., Friel, A. M., McDonnell, S., Crown, J., Radomski, M. W., & O’Driscoll, L. (2013) · European Journal of Cancer · doi:10.1016/j.ejca.2013.01.017 · Cited by 239
Argonaute 2 Complexes Selectively Protect the Circulating MicroRNAs in Cell-Secreted Microvesicles
Li, L., Zhu, D., Huang, L., Zhang, J., Bian, Z., Chen, X., Liu, Y., Zhang, C.-Y., & Zen, K. (2012) · PLoS ONE · doi:10.1371/journal.pone.0046957 · Cited by 216
Grelier, G., Voirin, N., Ay, A.-S., Cox, D. G., Chabaud, S., Treilleux, I., Léon-Goddard, S., Rimokh, R., Mikaelian, I., Venoux, C., et al. (2009) · British Journal of Cancer · doi:10.1038/sj.bjc.6605193 · Cited by 198
Aberrant Glycosylation as Biomarker for Cancer: Focus on CD43
Tuccillo, F. M., de Laurentiis, A., Palmieri, C., Fiume, G., Bonelli, P., Borrelli, A., Tassone, P., Scala, I., Buonaguro, F. M., Quinto, I., et al. (2014) · BioMed Research International · doi:10.1155/2014/742831 · Cited by 148
Shin, H., Morinaga, N., Noda, M., & Nakayama, K. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-05-0388 · Cited by 137
Itzkan, I., Qiu, L., Fang, H., Zaman, M. M., Vitkin, E. I., Ghiran, I. C., Salahuddin, S., Modell, M. D., Andersson, C., Kimerer, L. M., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0708669104 · Cited by 131
Self-citationOn Measuring miRNAs after Transient Transfection of Mimics or Antisense Inhibitors
Thomson, D. W., Bracken, C. P., Szubert, J. M., & Goodall, G. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0055214 · Cited by 122
Oncogenic miRNA-182-5p Targets Smad4 and RECK in Human Bladder Cancer
Hirata, H., Ueno, K., Shahryari, V., Tanaka, Y., Tabatabai, Z. L., Hinoda, Y., & Dahiya, R. (2012) · PLoS ONE · doi:10.1371/journal.pone.0051056 · Cited by 118
Confocal light absorption and scattering spectroscopic microscopy
Fang, H., Qiu, L., Vitkin, E. I., Zaman, M. M., Andersson, C., Salahuddin, S., Kimerer, L. M., Cipolloni, P. B., Modell, M. D., Turner, B. S., et al. (2007) · Applied Optics · doi:10.1364/ao.46.001760 · Cited by 55
Self-citationMirantes, C., Eritja, N., Dosil, M. A., Santacana, M., Pallarés, J., Gatius, S., Bergadà, L., Maiques, Ó., Matías‐Guiu, X., & Dolcet, X. (2013) · Disease Models & Mechanisms · doi:10.1242/dmm.011445 · Cited by 49
Cited by
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationThe biology , function , and biomedical applications of exosomes
Kalluri, R., & LeBleu, V. S. (2020) · Science · doi:10.1126/science.aau6977 · Cited by 10,770
“Exosomes derived from breast cancer and prostate cancer cells induce neoplasia through transfer of their miRNA cargo (148, 149).”
Self-citationBiological properties of extracellular vesicles and their physiological functions
Yáñez‐Mó, M., Siljander, P. R. ‐., Andreu, Z., Bedina Zavec, A., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27066 · Cited by 5,926
Communication by Extracellular Vesicles: Where We Are and Where We Need to Go
Tkach, M., & Théry, C. (2016) · Cell · doi:10.1016/j.cell.2016.01.043 · Cited by 3,444
Reassessment of exosome composition
Jeppesen, D. K., Fenix, A. M., Franklin, J. L., Higginbotham, J. N., Zhang, Q., Zimmerman, L. J., Liebler, D. C., Ping, J., Liu, Q., Evans, R., et al. (2019) · Cell · doi:10.1016/j.cell.2019.02.029 · Cited by 2,975
Metadata
- Authors
- Melo, S. A., Sugimoto, H., O’Connell, J. T., Kato, N., Villanueva, A., Vidal, A., Qiu, L., Vitkin, E., Perelman, L. T., Melo, C. A., Lucci, A., Ivan, C., Calin, G. A., & Kalluri, R.
- Venue
- Cancer Cell
- Publisher
- Elsevier BV
- Published
- 2014-11
- Volume
- 26
- Issue
- 5
- Pages
- 707-721
- ISSN
- 1535-6108 (ISSN-L)
- OpenAlex
- W2084770914
- Cited by
- 1,531
- References
- 57
- FWCI
- 47.94
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
