Single-Cell Transcriptomics and Fate Mapping of Ependymal Cells Reveals an Absence of Neural Stem Cell Function
Open accessAbstract
Ependymal cells are multi-ciliated cells that form the brain's ventricular epithelium and a niche for neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ). In addition, ependymal cells are suggested to be latent NSCs with a capacity to acquire neurogenic function. This remains highly controversial due to a lack of prospective in vivo labeling techniques that can effectively distinguish ependymal cells from neighboring V-SVZ NSCs. We describe a transgenic system that allows for targeted labeling of ependymal cells within the V-SVZ. Single-cell RNA-seq revealed that ependymal cells are enriched for cilia-related genes and share several stem-cell-associated genes with neural stem or progenitors. Under in vivo and in vitro neural-stem- or progenitor-stimulating environments, ependymal cells failed to demonstrate any suggestion of latent neural-stem-cell function. These findings suggest remarkable stability of ependymal cell function and provide fundamental insights into the molecular signature of the V-SVZ niche.
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The diversity of embryonic glia and neuronal populations observed in scRNA-seq was confirmed by snRNA-seq and expanded to adult and aged ChP.
“The diversity of embryonic glia and neuronal populations observed in scRNA-seq was confirmed by snRNA-seq and expanded to adult and aged ChP (Figure S2D,E) (Dulken et al., 2017; Mizrak et al., 2019; Shah et al., 2018).”
From A cellular and spatial map of the choroid plexus across brain ventricles and ages (2021) · cites this paper as Shah et al., 2018
Recently published transcriptomic studies showed aging-related downregulation of ribosomal genes in ASC and NSC, and upregulation in MG of aged and diseased brains.
“recently published transcriptomic studies showed an agingrelated downregulation of ribosomal genes in both ASC 14 and NSC 61 , and an upregulation in MG of both aged 62,63 and diseased brains 62,64 .”
From Single-cell transcriptomic profiling of the aging mouse brain (2019) · cites this paper as [62]
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- Authors
- Shah, P. T., Stratton, J. A., Stykel, M. G., Abbasi, S., Sharma, S., Mayr, K. A., Koblinger, K., Whelan, P. J., & Biernaskie, J.
- Affiliations
- University of Calgary (CA)
- Corresponding author
- Biernaskie, Jeff
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2018-05
- Volume
- 173
- Issue
- 4
- Pages
- 1045-1057.e9
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2802670298
- Cited by
- 221
- References
- 43
- Citations by year
- 2026: 20 · 2025: 27 · 2024: 34 · 2023: 19 · 2022: 30 · 2021: 31 · 2020: 29 · 2019: 25 · 2018: 6
- FWCI
- 8.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Alberta Children’s Hospital Research Institute
- Markin Undergraduate Research Program
- Canadian Institutes of Health Research (MOP106646, MOP130475)
- CIHR New Investigator award
- Calgary Firefighters Burn Treatment Society Chair in Skin Regeneration and Wound Healing
- University of Calgary QEII award
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Keywords
- ependymal cells, neural stem cells, ventricular-subventricular zone, fate mapping, single-cell RNA sequencing, ciliated cells, stem cell niche, neurogenesis
- MeSH terms
- Actins, Animals, Cell Differentiation, Ependyma, Female, Male, Mice, Inbred C57BL, Fibroblast Growth Factor 2, Platelet Endothelial Cell Adhesion Molecule-1, Lateral Ventricles, Genomics, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factor A, Mice, Stem Cell Niche, Neural Stem Cells, Single-Cell Analysis, Transcriptome
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