Cited by 183Neurogenesis and neuroplasticity mechanismsSingle-cell and spatial transcriptomicsDevelopmental Biology and Gene Regulation

Single-Cell Transcriptomics Characterizes Cell Types in the Subventricular Zone and Uncovers Molecular Defects Impairing Adult Neurogenesis

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Zywitza, Vera, Misios, Aristotelis, Bunatyan, Lena, Willnow, Thomas E., Rajewsky, Nikolaus·2018·Cell Reports

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Abstract

Neural stem cells (NSCs) contribute to plasticity and repair of the adult brain. Niches harboring NSCs regulate stem cell self-renewal and differentiation. We used comprehensive and untargeted single-cell RNA profiling to generate a molecular cell atlas of the largest germinal region of the adult mouse brain, the subventricular zone (SVZ). We characterized >20 neural and non-neural cell types and gained insights into the dynamics of neurogenesis by predicting future cell states based on computational analysis of RNA kinetics. Furthermore, we applied our single-cell approach to document decreased numbers of NSCs, reduced proliferation activity of progenitors, and perturbations in Wnt and BMP signaling pathways in mice lacking LRP2, an endocytic receptor required for SVZ maintenance. Our data provide a valuable resource to study adult neurogenesis and a proof of principle for the power of single-cell RNA sequencing to elucidate neural cell-type-specific alterations in loss-of-function models.

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Crossref · 10.1016/j.celrep.2018.11.003

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  • Recently published transcriptomic studies showed aging-related downregulation of ribosomal genes in ASC and NSC, and upregulation in MG of aged and diseased brains.

    “recently published transcriptomic studies showed an agingrelated downregulation of ribosomal genes in both ASC 14 and NSC 61 , and an upregulation in MG of both aged 62,63 and diseased brains 62,64 .”

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Authors
Zywitza, V., Misios, A., Bunatyan, L., Willnow, T. E., & Rajewsky, N.
Affiliations
Max Delbrück Center (DE)
Corresponding author
  • Willnow, Thomas E.
  • Rajewsky, Nikolaus
Venue
Cell Reports
Publisher
Elsevier BV
Published
2018-11
Volume
25
Issue
9
Pages
2457-2469.e8
ISSN
2211-1247 (ISSN-L)
OpenAlex
W2813292791
Cited by
183
References
60
Citations by year
2026: 7 · 2025: 18 · 2024: 16 · 2023: 30 · 2022: 32 · 2021: 22 · 2020: 37 · 2019: 20 · 2018: 1
FWCI
7.37
Type
journal article
Language
en
Open access
Locations
  • Cell Reports (publishedVersion, cc-by-nc-nd)
  • SSRN Electronic Journal (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (acceptedVersion)
  • MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
Funders
  • European Union
  • Marie Sklodowska-Curie Actions (721890)
  • Chan Zuckerberg Initiative
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
subventricular zone, adult neurogenesis, neural stem cells, cell types, cell atlas, RNA kinetics, Wnt signaling, BMP signaling, megalin, endocytic receptor, stem cell niche, progenitor cell proliferation
MeSH terms
Aging, Animals, Gene Expression Regulation, Mice, Inbred C57BL, Models, Biological, RNA, Dentate Gyrus, Cell Lineage, Lateral Ventricles, Low Density Lipoprotein Receptor-Related Protein-2, Cell Proliferation, Stem Cell Niche, Neurogenesis, Neural Stem Cells, Single-Cell Analysis, Transcriptome, RNA, Long Noncoding

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