Single-cell transcriptomic analysis defines heterogeneity and transcriptional dynamics in the adult neural stem cell lineage
Open accessAbstract
Neural stem cells (NSCs) in the adult mammalian brain serve as a reservoir for the generation of new neurons, oligodendrocytes, and astrocytes. Here, we use single-cell RNA sequencing to characterize adult NSC populations and examine the molecular identities and heterogeneity of in vivo NSC populations. We find that cells in the NSC lineage exist on a continuum through the processes of activation and differentiation. Interestingly, rare intermediate states with distinct molecular profiles can be identified and experimentally validated, and our analysis identifies putative surface markers and key intracellular regulators for these subpopulations of NSCs. Finally, using the power of single-cell profiling, we conduct a meta-analysis to compare in vivo NSCs and in vitro cultures, distinct fluorescence-activated cell sorting strategies, and different neurogenic niches. These data provide a resource for the field and contribute to an integrative understanding of the adult NSC lineage.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Overlapping TF gradients accompany cellular differentiation in systems including exit from pluripotency, organogenesis, and tissue regeneration by adult stem cells.
“Similar overlapping TF gradients accompany cellular differentiation in systems ranging from the exit from pluripotency5 over organogenesis6 to tissue regeneration by other adult stem cells.7,8”
From Design principles of cell-state-specific enhancers in hematopoiesis (2025) · cites this paper as [8]
Previous low-throughput single-cell studies have begun to illuminate the stages of qNSC activation in the adult V-SVZ, focusing on NSCs purified by FACS from the lateral wall and the neuronal lineage.
“Previous single-cell studies using low-throughput approaches have begun to illuminate the stages of qNSC activation in the adult V-SVZ (Basak et al., 2018; Dulken et al., 2017; Llorens-Bobadilla et al., 2015; Luo et al., 2015), focusing on NSCs purified by fluorescence-activated cell sorting (FACS) from the lateral wall and the neuronal lineage.”
From Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages (2019) · cites this paper as Dulken et al., 2017
GSEA revealed a clear correspondence between the neuronal lineage trajectory and previously published studies with lower throughput.
“GSEA revealed a clear correspondence between our neuronal lineage trajectory and previously published studies with lower throughput (Figures S2B and S2C; Dulken et al., 2017; Llorens-Bobadilla et al., 2015).”
From Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages (2019) · cites this paper as Dulken et al., 2017
All profiling studies in the adult V-SVZ, including recent low-throughput single-cell studies, have focused only on the lateral wall and the neuronal lineage.
“Although transcriptome analysis in the postnatal V-SVZ has uncovered molecular differences between the dorsal and the lateral walls (Azim et al., 2015), all profiling studies in the adult V-SVZ, including recent low-throughput, single-cell studies, have focused only on the lateral wall and the neuronal lineage (Basak et al., 2018; Codega et al., 2014; Dulken et al., 2017; Llorens-Bobadilla et al.,”
From Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages (2019) · cites this paper as Dulken et al., 2017
Top markers that correlated with both NPCs and OPCs included S100a6, Notum, Dbi, and Sfrp1, as well as known V-SVZ qNSC activation and injury-response markers, such as Ascl1, Vim, Thbs4, and Rpl32.
“Top markers that correlated with both NPCs and OPCs included S100a6, Notum, Dbi, and Sfrp1, as well as known V-SVZ qNSC activation and injury-response markers, such as Ascl1, Vim, Thbs4, and Rpl32 (Benner et al., 2013; Dulken et al., 2017; Llorens-Bobadilla et al., 2015; Figure 5B).”
From Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages (2019) · cites this paper as Dulken et al., 2017
The diversity of embryonic glia and neuronal populations observed in scRNA-seq was confirmed by snRNA-seq and expanded to adult and aged ChP.
“The diversity of embryonic glia and neuronal populations observed in scRNA-seq was confirmed by snRNA-seq and expanded to adult and aged ChP (Figure S2D,E) (Dulken et al., 2017; Mizrak et al., 2019; Shah et al., 2018).”
From A cellular and spatial map of the choroid plexus across brain ventricles and ages (2021) · cites this paper as Dulken et al., 2017
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Trapnell, C., Cacchiarelli, D., Grimsby, J., Pokharel, P., Li, S., Morse, M., Lennon, N. J., Livak, K. J., Mikkelsen, T. S., & Rinn, J. L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2859 · Cited by 7,314 · see citing works
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets.
Macosko, E. Z., Basu, A., Satija, R., Nemesh, J., Shekhar, K., Goldman, M., Tirosh, I., Bialas, A. R., Kamitaki, N., Martersteck, E. M., et al. (2015) · Cell · doi:10.1016/j.cell.2015.05.002 · Cited by 7,110 · see citing works
Friedman, J. H. (2002) · Computational Statistics & Data Analysis · doi:10.1016/s0167-9473(01)00065-2 · Cited by 7,067 · see citing works
Genome-wide atlas of gene expression in the adult mouse brain
Lein, E. S., Hawrylycz, M. J., Ao, N., Ayres, M., Bensinger, A., Bernard, A., Boe, A. F., Boguski, M. S., Brockway, K. S., Byrnes, E. J., et al. (2007) · Nature · doi:10.1038/nature05453 · Cited by 5,572 · see citing works
Subventricular zone astrocytes are neural stem cells in the adult mammalian brain
Doetsch, F., Caillé, I., Lim, D. A., García-Verdugo, J. M., & Alvarez-Buylla, A. (1999) · Cell · doi:10.1016/s0092-8674(00)80783-7 · Cited by 3,898 · see citing works
Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells
Klein, A. M., Mazutis, L., Akartuna, I., Tallapragada, N., Veres, A., Li, V., Peshkin, L., Weitz, D. A., & Kirschner, M. W. (2015) · Cell · doi:10.1016/j.cell.2015.04.044 · Cited by 3,356 · see citing works
Cahoy, J. D., Emery, B., Kaushal, A., Foo, L. C., Zamanian, J. L., Christopherson, K. S., Xing, Y., Lubischer, J. L., Krieg, P. A., Krupenko, S. A., et al. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.4178-07.2008 · Cited by 3,129 · see citing works
Mechanisms and Functional Implications of Adult Neurogenesis
Zhao, C., Deng, W., & Gage, F. H. (2008) · Cell · doi:10.1016/j.cell.2008.01.033 · Cited by 3,007 · see citing works
Bayesian approach to single-cell differential expression analysis
Kharchenko, P. V., Silberstein, L., & Scadden, D. T. (2014) · Nature Methods · doi:10.1038/nmeth.2967 · Cited by 1,348 · see citing works
EGF Converts Transit-Amplifying Neurogenic Precursors in the Adult Brain into Multipotent Stem Cells
Doetsch, F. K., Petreanu, L., Caillé, I., García‐Verdugo, J. M., & Álvarez-Buylla, A. (2002) · Neuron · doi:10.1016/s0896-6273(02)01133-9 · Cited by 1,048 · see citing works
Mirzadeh, Z., Merkle, F. T., Soriano-Navarro, M., Garcia-Verdugo, J. M., & Alvarez-Buylla, A. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.07.004 · Cited by 1,015 · see citing works
Garcia, A. D. R., Doan, N., Imura, T., Bush, T. G., & Sofroniew, M. V. (2004) · Nature Neuroscience · doi:10.1038/nn1340 · Cited by 990 · see citing works
Pollen, A. A., Nowakowski, T. J., Shuga, J., Wang, X., Leyrat, A. A., Lui, J. H., Li, N., Szpankowski, L., Fowler, B., Chen, P., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.2967 · Cited by 920 · see citing works
Single-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis
Shin, J., Berg, D. A., Zhu, Y., Shin, J. Y., Song, J., Bonaguidi, M. A., Enikolopov, G., Nauen, D. W., Christian, K. M., Ming, G.-L., et al. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.07.013 · Cited by 784 · see citing works
Llorens-Bobadilla, E., Zhao, S., Baser, A., Saiz-Castro, G., Zwadlo, K., & Martin-Villalba, A. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.07.002 · Cited by 778 · see citing works
Quantitative assessment of single-cell RNA-sequencing methods
Wu, A. R., Neff, N. F., Kalisky, T., Dalerba, P., Treutlein, B., Rothenberg, M. E., Mburu, F. M., Mantalas, G. L., Sim, S., Clarke, M. F., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2694 · Cited by 696 · see citing works
Hitoshi, S., Alexson, T. O., Tropepe, V., Donoviel, D. B., Elia, A., Nye, J. S., Conlon, R. A., Mak, T. W., Bernstein, A., & van der Kooy, D. (2002) · Genes & Development · doi:10.1101/gad.975202 · Cited by 690 · see citing works
Diffusion maps for high-dimensional single-cell analysis of differentiation data
Haghverdi, L., Buettner, F., & Theis, F. J. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv325 · Cited by 649 · see citing works
Codega, P., Silva-Vargas, V., Paul, A., Maldonado-Soto, A. R., DeLeo, A. M., Pastrana, E., & Doetsch, F. (2014) · Neuron · doi:10.1016/j.neuron.2014.02.039 · Cited by 634 · see citing works
Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons
Habib, N., Li, Y., Heidenreich, M., Swiech, L., Avraham-Davidi, I., Trombetta, J. J., Hession, C., Zhang, F., & Regev, A. (2016) · Science · doi:10.1126/science.aad7038 · Cited by 601 · see citing works
Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq
Cadwell, C. R., Palasantza, A., Jiang, X., Berens, P., Deng, Q., Yilmaz, M., Reimer, J., Shen, S., Bethge, M., Tolias, K. F., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3445 · Cited by 581 · see citing works
Mira, H., Andreu, Z., Suh, H., Lie, D. C., Jessberger, S., Consiglio, A., San Emeterio, J., Hortigüela, R., Marqués-Torrejón, M. Á., Nakashima, K., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.04.016 · Cited by 480 · see citing works
Combinatorial labeling of single cells for gene expression cytometry
Fan, H. C., Fu, G. K., & Fodor, S. P. A. (2015) · Science · doi:10.1126/science.1258367 · Cited by 455 · see citing works
Pastrana, É., Cheng, L.-C., & Doetsch, F. K. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0810407106 · Cited by 381 · see citing works
Petryniak, M. A., Potter, G. B., Rowitch, D. H., & Rubenstein, J. L. R. (2007) · Neuron · doi:10.1016/j.neuron.2007.06.036 · Cited by 371 · see citing works
Live Astrocytes Visualized by Green Fluorescent Protein in Transgenic Mice
Zhuo, L., Sun, B., Zhang, C.-L., Fine, A., Chiu, S. Y., & Messing, A. (1997) · Developmental Biology · doi:10.1006/dbio.1997.8601 · Cited by 364 · see citing works
Beckervordersandforth, R., Tripathi, P., Ninkovic, J., Bayam, E., Lepier, A., Stempfhuber, B., Kirchhoff, F., Hirrlinger, J., Haslinger, A., Lie, D. C., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.11.017 · Cited by 335 · see citing works
Neural stem cell systems: physiological players or in vitro entities?
Conti, L., & Cattaneo, E. F. (2010) · Nature reviews. Neuroscience · doi:10.1038/nrn2761 · Cited by 319 · see citing works
Adult neural stem cells in distinct microdomains generate previously unknown interneuron types
Merkle, F. T., Fuentealba, L. C., Sanders, T. A., Magno, L., Kessaris, N., & Álvarez-Buylla, A. (2013) · Nature Neuroscience · doi:10.1038/nn.3610 · Cited by 277 · see citing works
Single-Cell Transcriptome Analyses Reveal Signals to Activate Dormant Neural Stem Cells
Luo, Y., Coskun, V., Liang, A., Yu, J., Cheng, L., Ge, W., Shi, Z., Zhang, K., Li, C., Cui, Y., et al. (2015) · Cell · doi:10.1016/j.cell.2015.04.001 · Cited by 263 · see citing works
Noggin Expands Neural Stem Cells in the Adult Hippocampus
Bonaguidi, M. A., Peng, C., McGuire, T. L., Falciglia, G. H., Gobeske, K. T., Czeisler, C. M., & Kessler, J. A. (2008) · Journal of Neuroscience · doi:10.1523/jneurosci.3314-07.2008 · Cited by 254 · see citing works
Waclaw, R. R., Allen, Z. J., Bell, S. M., Erdélyi, F., Szabó, G., Potter, S. S., & Campbell, K. (2006) · Neuron · doi:10.1016/j.neuron.2006.01.018 · Cited by 237 · see citing works
Andersen, J., Urbán, N., Achimastou, A., Ito, A., Simic, M., Ullom, K., Martynoga, B., Lebel, M., Göritz, C., Frisén, J., et al. (2014) · Neuron · doi:10.1016/j.neuron.2014.08.004 · Cited by 212 · see citing works
Orchestrating transcriptional control of adult neurogenesisPreprint
Hsieh, J. (2012) · Genes & Development · doi:10.1101/gad.187336.112 · Cited by 208 · see citing works
Jagged1 signals in the postnatal subventricular zone are required for neural stem cell self‐renewal
Nyfeler, Y., Kirch, R. D., Mantei, N., Leone, D. P., Radtke, F., Suter, U., & Taylor, V. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600816 · Cited by 191 · see citing works
Fast clonal expansion and limited neural stem cell self-renewal in the adult subependymal zone
Calzolari, F., Michel, J., Baumgart, E. V., Theis, F. J., Götz, M., & Ninkovic, J. (2015) · Nature Neuroscience · doi:10.1038/nn.3963 · Cited by 186 · see citing works
Somatic stem cell heterogeneity: diversity in the blood, skin and intestinal stem cell compartments
Goodell, M., Nguyen, H., & Shroyer, N. (2015) · Nature reviews. Molecular cell biology · doi:10.1038/nrm3980 · Cited by 166 · see citing works
Long, J. E., Swan, C. H., Liang, W. S., Cobos, I., Potter, G. B., & Rubenstein, J. L. R. (2008) · The Journal of Comparative Neurology · doi:10.1002/cne.21854 · Cited by 156 · see citing works
Mich, J. K., Signer, R. A. J., Nakada, D., Pineda, A., Burgess, R. J., Vue, T. Y., Johnson, J. E. B., & Morrison, S. J. (2014) · eLife · doi:10.7554/elife.02669 · Cited by 151 · see citing works
Maric, D., Fiorio, A., Chang, Y. H., & Barker, J. L. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.5141-06.2007 · Cited by 127 · see citing works
Expression profile of an operationally-defined neural stem cell clone
Parker, M. A., Anderson, J. K., Corliss, D. A., Abraria, V. E., Sidman, R. L., Park, K. I., Teng, Y. D., Cotanche, D. A., & Snyder, E. Y. (2005) · Experimental Neurology · doi:10.1016/j.expneurol.2005.04.018 · Cited by 120 · see citing works
Erratum: Neural stem cell systems: physiological players or in vitro entities?
Conti, L., & Cattaneo, E. F. (2010) · Nature reviews. Neuroscience · doi:10.1038/nrn2938 · Cited by 113 · see citing works
SIRT1 suppresses self-renewal of adult hippocampal neural stem cells
Ma, C., Yao, M., Zhai, Q., Jiao, J., Yuan, X., & Poo, M. (2014) · Development · doi:10.1242/dev.117937 · Cited by 87 · see citing works
Prospective isolation of adult neural stem cells from the mouse subependymal zone
Fischer, J., Beckervordersandforth, R., Tripathi, P., Steiner-Mezzadri, A., Ninkovic, J., & Götz, M. (2011) · Nature Protocols · doi:10.1038/nprot.2011.412 · Cited by 71 · see citing works
Interaction between DLX2 and EGFR regulates proliferation and neurogenesis of SVZ precursors
Suh, Y., Obernier, K., Hölzl-Wenig, G., Mandl, C., Herrmann, A., Wörner, K., Eckstein, V., & Ciccolini, F. (2009) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2009.08.003 · Cited by 51 · see citing works
Div-Seq: A single nucleus RNA-Seq method reveals dynamics of rare adult newborn neurons in the CNSPreprint
Habib, N., Li, Y., Heidenreich, M., Swiech, L. J., Trombetta, J. J., Zhang, F., & Regev, A. (2016) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/045989 · Cited by 11 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Leeman, D. S., Hebestreit, K., Ruetz, T., Webb, A. E., McKay, A., Pollina, E. A., Dulken, B. W., Zhao, X., Yeo, R. W., Ho, T. T., et al. (2018) · Science · doi:10.1126/science.aag3048 · Cited by 528 · see citing works
Neural stem cells: origin, heterogeneity and regulation in the adult mammalian brain
Obernier, K., & Alvarez-Buylla, A. (2019) · Development · doi:10.1242/dev.156059 · Cited by 578 · see citing works
Single-cell analysis reveals T cell infiltration in old neurogenic niches
Dulken, B. W., Buckley, M. T., Navarro Negredo, P., Saligrama, N., Cayrol, R., Leeman, D. S., George, B. M., Boutet, S. C., Hebestreit, K., Pluvinage, J. V., et al. (2019) · Nature · doi:10.1038/s41586-019-1362-5 · Cited by 521 · see citing works
Self-citationQuiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain
Kalamakis, G., Brüne, D., Ravichandran, S., Bolz, J., Fan, W., Ziebell, F., Stiehl, T., Catalá-Martinez, F., Kupke, J., Zhao, S., et al. (2019) · Cell · doi:10.1016/j.cell.2019.01.040 · Cited by 399 · see citing works
A cellular and spatial map of the choroid plexus across brain ventricles and ages
Dani, N., Herbst, R. H., McCabe, C., Green, G. S., Kaiser, K., Head, J. P., Cui, J., Shipley, F. B., Jang, A., Dionne, D., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.003 · Cited by 388 · see citing works
Single-Cell Transcriptomics Meets Lineage Tracing
Kester, L., & van Oudenaarden, A. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.04.014 · Cited by 375 · see citing works
Quiescence of Adult Mammalian Neural Stem Cells: A Highly Regulated Rest
Urbán, N., Blomfield, I. M., & Guillemot, F. (2019) · Neuron · doi:10.1016/j.neuron.2019.09.026 · Cited by 370 · see citing works
Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures
Sousa, C., Golebiewska, A., Poovathingal, S. K., Kaoma, T., Pires‐Afonso, Y., Martina, S., Coowar, D., Azuaje, F., Skupin, A., Balling, R., et al. (2018) · The EMBO Reports · doi:10.15252/embr.201846171 · Cited by 281 · see citing works
Ecker, J. R., Geschwind, D. H., Kriegstein, A. R., Ngai, J., Osten, P., Polioudakis, D., Regev, A., Šestan, N., Wickersham, I. R., & Zeng, H. (2017) · Neuron · doi:10.1016/j.neuron.2017.10.007 · Cited by 254 · see citing works
Artegiani, B., Lyubimova, A., Muraro, M., van Es, J. H., van Oudenaarden, A., & Clevers, H. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.050 · Cited by 259 · see citing works
Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
Mizrak, D., Levitin, H. M., Delgado, A. C., Crotet, V., Yuan, J., Chaker, Z., Silva-Vargas, V., Sims, P. A., & Doetsch, F. (2019) · Cell Reports · doi:10.1016/j.celrep.2018.12.044 · Cited by 248 · see citing works
Adult Neurogenesis Is Sustained by Symmetric Self-Renewal and Differentiation
Obernier, K., Cebrian-Silla, A., Thomson, M., Parraguez, J. I., Anderson, R., Guinto, C., Rodas Rodriguez, J., Garcia-Verdugo, J.-M., & Alvarez-Buylla, A. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.01.003 · Cited by 250 · see citing works
Aging and Rejuvenation of Neural Stem Cells and Their Niches
Navarro Negredo, P., Yeo, R. W., & Brunet, A. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2020.07.002 · Cited by 246 · see citing works
Self-citationAn Immunocompetent Mouse Model of Zika Virus Infection
Gorman, M. J., Caine, E. A., Zaitsev, K., Begley, M., Weger‐Lucarelli, J., Uccellini, M. B., Tripathi, S., Morrison, J. M., Yount, B. L., Dinnon, K. H., et al. (2018) · Cell Host & Microbe · doi:10.1016/j.chom.2018.04.003 · Cited by 240 · see citing works
Shah, P. T., Stratton, J. A., Stykel, M. G., Abbasi, S., Sharma, S., Mayr, K. A., Koblinger, K., Whelan, P. J., & Biernaskie, J. (2018) · Cell · doi:10.1016/j.cell.2018.03.063 · Cited by 221 · see citing works
Weng, Q., Wang, J., Wang, J., He, D., Cheng, Z., Zhang, F., Verma, R., Xu, L., Dong, X., Liao, Y., et al. (2019) · Cell stem cell · doi:10.1016/j.stem.2019.03.006 · Cited by 211 · see citing works
Xu, R., Li, X., Boreland, A. J., Posyton, A., Kwan, K. Y., Hart, R. P., & Jiang, P. (2020) · Nature Communications · doi:10.1038/s41467-020-15411-9 · Cited by 190 · see citing works
Troy+ brain stem cells cycle through quiescence and regulate their number by sensing niche occupancy
Basak, O., Krieger, T. G., Muraro, M. J., Wiebrands, K., Stange, D. E., Frias-Aldeguer, J., Rivron, N. C., van de Wetering, M., van Es, J. H., van Oudenaarden, A., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1715911114 · Cited by 186 · see citing works
N-Myc–mediated epigenetic reprogramming drives lineage plasticity in advanced prostate cancer
Berger, A., Brady, N. J., Bareja, R., Robinson, B., Conteduca, V., Augello, M. A., Puca, L., Ahmed, A., Dardenne, É., Lü, X., et al. (2019) · Journal of Clinical Investigation · doi:10.1172/jci127961 · Cited by 189 · see citing works
Zywitza, V., Misios, A., Bunatyan, L., Willnow, T. E., & Rajewsky, N. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.11.003 · Cited by 183 · see citing works
Amplification of human interneuron progenitors promotes brain tumors and neurological defects
Eichmüller, O. L., Corsini, N. S., Vértesy, Á., Morassut, I., Scholl, T., Gruber, V.-E., Peer, A. M., Chu, J., Novatchkova, M., Hainfellner, J. A., et al. (2022) · Science · doi:10.1126/science.abf5546 · Cited by 163 · see citing works
Stem cell quiescence: the challenging path to activation
Urbán, N., & Cheung, T. H. (2021) · Development · doi:10.1242/dev.165084 · Cited by 154 · see citing works
Cebrián‐Silla, A., Nascimento, M. A., Redmond, S., Mansky, B., Wu, D., Obernier, K., Rodriguez, R. R., González-Granero, S., García‐Verdugo, J. M., Lim, D. A., et al. (2021) · eLife · doi:10.7554/elife.67436 · Cited by 137 · see citing works
Early stem cell aging in the mature brain
Ibrayeva, A., Bay, M., Pu, E., Jörg, D. J., Peng, L., Jun, H., Zhang, N., Aaron, D., Lin, C., Resler, G., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.03.018 · Cited by 137 · see citing works
Adult Neural Stem Cells Are Alerted by Systemic Inflammation through TNF-α Receptor Signaling
Belenguer, G., Duart-Abadía, P., Jordán‐Pla, A., Domingo-Muelas, A., Blasco-Chamarro, L., Ferrón, S. R., Morante‐Redolat, J. M., & Fariñas, I. (2020) · Cell stem cell · doi:10.1016/j.stem.2020.10.016 · Cited by 130 · see citing works
Deciphering Metabolic Heterogeneity by Single-Cell Analysis
Evers, T. M. J., Hochane, M., Tans, S. J., Heeren, R. M. A., Semrau, S., Nemes, P., & Mashaghi, A. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b02410 · Cited by 121 · see citing works
Glial cell diversity and methamphetamine-induced neuroinflammation in human cerebral organoids
Dang, J. W., Tiwari, S. K., Agrawal, K., Hui, H., Qin, Y., & Rana, T. M. (2020) · Molecular Psychiatry · doi:10.1038/s41380-020-0676-x · Cited by 123 · see citing works
An Introduction to the Analysis of Single-Cell RNA-Sequencing Data
Aljanahi, A. A., Danielsen, M., & Dunbar, C. E. (2018) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2018.07.003 · Cited by 116 · see citing works
Lieberman, Y., Rokach, L., & Shay, T. (2018) · PLoS ONE · doi:10.1371/journal.pone.0205499 · Cited by 113 · see citing works
Transcriptome dynamics of hippocampal neurogenesis in macaques across the lifespan and aged humans
Wang, W., Wang, M., Yang, M., Zeng, B., Qiu, W., Ma, Q., Jing, X., Zhang, Q., Wang, B., Yin, C., et al. (2022) · Cell Research · doi:10.1038/s41422-022-00678-y · Cited by 121 · see citing works
Autocrine Mfge8 Signaling Prevents Developmental Exhaustion of the Adult Neural Stem Cell Pool
Zhou, Y. F., Bond, A. M., Shade, J. E., Zhu, Y., Davis, C.-H. O., Wang, X., Su, Y., Yoon, K., Phan, A. T., Chen, W. J., et al. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.08.005 · Cited by 112 · see citing works
The issue of the multipotency of the neural crest cells
Dupin, É., Calloni, G. W., Coelho‐Aguiar, J. M., & Douarin, N. M. L. (2018) · Developmental Biology · doi:10.1016/j.ydbio.2018.03.024 · Cited by 105 · see citing works
Wang, X., Zhou, R., Xiong, Y., Zhou, L., Yan, X., Wang, M., Fan, L., Xie, C., Zhang, Y., Huang, Z., et al. (2021) · Cell Research · doi:10.1038/s41422-020-00451-z · Cited by 96 · see citing works
Gengatharan, A., Malvaut, S., Marymonchyk, A., Ghareghani, M., Snapyan, M., Fischer-Sternjak, J., Ninkovic, J., Götz, M., & Saghatelyan, A. (2021) · Cell · doi:10.1016/j.cell.2020.12.026 · Cited by 97 · see citing works
Novel Galectin-3 Roles in Neurogenesis, Inflammation and Neurological Diseases
Soares, L. C., Al‐Dalahmah, O., Hillis, J. M., Young, C. C., Asbed, I., Sakaguchi, M., O’Neill, E. E., & Szele, F. G. (2021) · Cells · doi:10.3390/cells10113047 · Cited by 94 · see citing works
Spatial transcriptomic clocks reveal cell proximity effects in brain ageing
Sun, E., Zhou, O. Y., Hauptschein, M., Rappoport, N., Xu, L., Negredo, P. N., Liu, L. A., Rando, T. A., Zou, J., & Brunet, A. (2024) · Nature · doi:10.1038/s41586-024-08334-8 · Cited by 98 · see citing works
Chavali, M., Klingener, M., Kokkosis, A. G., Garkun, Y., Felong, S., Maffei, A., & Aguirre, A. A. (2017) · Nature Communications · doi:10.1038/s41467-017-02440-0 · Cited by 85 · see citing works
Regulation of neurogenesis in the adult and aging brain
Katsimpardi, L., & Lledo, P. (2018) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2018.07.006 · Cited by 85 · see citing works
Morizur, L., Chicheportiche, A., Gauthier, L., Daynac, M., Boussin, F. D., & Mouthon, M. (2018) · Stem Cell Reports · doi:10.1016/j.stemcr.2018.06.005 · Cited by 81 · see citing works
scDFC: A deep fusion clustering method for single-cell RNA-seq data
Hu, D., Liang, K., Zhou, S., Tu, W., Liu, M., & Liu, X. (2023) · Briefings in Bioinformatics · doi:10.1093/bib/bbad216 · Cited by 80 · see citing works
Morrison, J. A., McLennan, R., Wolfe, L. A., Gogol, M., Meier, S. R., McKinney, M. C., Teddy, J. M., Holmes, L., Semerad, C. L., Box, A., et al. (2017) · eLife · doi:10.7554/elife.28415 · Cited by 75 · see citing works
Adult Neurogenesis and the Promise of Adult Neural Stem Cells
Ghosh, H. S. (2019) · Journal of Experimental Neuroscience · doi:10.1177/1179069519856876 · Cited by 77 · see citing works
Brain Transcriptome Databases: A User's Guide
Keil, J. M., Qalieh, A., & Kwan, K. Y. (2018) · Journal of Neuroscience · doi:10.1523/jneurosci.1930-17.2018 · Cited by 74 · see citing works
North, H. F., Weissleder, C., Fullerton, J. M., Sager, R., Webster, M. J., & Weickert, C. S. (2021) · Translational Psychiatry · doi:10.1038/s41398-021-01742-8 · Cited by 75 · see citing works
Lepko, T., Pusch, M., Müller, T., Schulte, D., Ehses, J., Kiebler, M. A., Hasler, J., Huttner, H. B., Vandenbroucke, R. E., Vandendriessche, C., et al. (2019) · The EMBO Journal · doi:10.15252/embj.2018100481 · Cited by 72 · see citing works
Dillen, Y., Kemps, H., Gervois, P., Wolfs, E., & Bronckaers, A. (2019) · Translational Stroke Research · doi:10.1007/s12975-019-00717-8 · Cited by 75 · see citing works
Adult Human Glioblastomas Harbor Radial Glia-like Cells
Wang, R., Sharma, R., Shen, X., Laughney, A. M., Funato, K., Clark, P. J., Shpokayte, M., Morgenstern, P. F., Navare, M., Xu, Y., et al. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2020.01.007 · Cited by 71 · see citing works
Quiescent Neural Stem Cells for Brain Repair and Regeneration: Lessons from Model Systems
Otsuki, L., & Brand, A. H. (2020) · Trends in Neurosciences · doi:10.1016/j.tins.2020.02.002 · Cited by 68 · see citing works
The mitochondrial unfolded protein response regulates hippocampal neural stem cell aging
Wang, C.-L., Ohkubo, R., Mu, W., Chen, W., Fan, J. L., Song, Z., Maruichi, A., Sudmant, P. H., Pisco, A. O., Dubal, D. B., et al. (2023) · Cell Metabolism · doi:10.1016/j.cmet.2023.04.012 · Cited by 72 · see citing works
Borrett, M. J., Innes, B. T., Jeong, D., Tahmasian, N., Storer, M. A., Bader, G. D., Kaplan, D. R., & Miller, F. D. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108022 · Cited by 69 · see citing works
Neurogenesis From Embryo to Adult – Lessons From Flies and Mice
Mira, H., & Morante, J. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00533 · Cited by 64 · see citing works
Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter
Bocchi, R., Thorwirth, M., Simon‐Ebert, T., Koupourtidou, C., Clavreul, S., Kolf, K., Vecchia, P. D., Bottes, S., Jessberger, S., Zhou, J., et al. (2025) · Nature Neuroscience · doi:10.1038/s41593-025-01878-6 · Cited by 66 · see citing works
Single‐cell RNA‐sequencing of the brain
Durán, R. C., Wei, H., & Wu, J. Q. (2017) · Clinical and Translational Medicine · doi:10.1186/s40169-017-0150-9 · Cited by 63 · see citing works
Translating neural stem cells to neurons in the mammalian brain
Zahr, S. K., Kaplan, D. R., & Miller, F. D. (2019) · Cell Death and Differentiation · doi:10.1038/s41418-019-0411-9 · Cited by 63 · see citing works
A single factor elicits multilineage reprogramming of astrocytes in the adult mouse striatum
Zhang, Y., Li, B., Cananzi, S. G., Han, C., Wang, L., Zou, Y., Fu, Y., Hon, G. C., & Zhang, C. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2107339119 · Cited by 63 · see citing works
Adult Neural Stem Cells: Born to Last
Morales, A. V., & Mira, H. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2019.00096 · Cited by 59 · see citing works
Mao, X., An, Q., Xi, H., Yang, X.-J., Zhang, X., Yuan, S., Wang, J., Hu, Y., Liu, Q., & Fan, G. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.08.012 · Cited by 60 · see citing works
Deciphering Brain Complexity using Single-Cell Sequencing
Mu, Q., Chen, Y., & Wang, J. (2019) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2018.07.007 · Cited by 60 · see citing works
Chen, L., Li, J., Zhang, Y., Feng, K., Wang, S., Zhang, Y., Huang, T., Kong, X., & Cai, Y. (2017) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26507 · Cited by 59 · see citing works
Conserved and Divergent Features of Adult Neurogenesis in Zebrafish
Labusch, M., Mancini, L., Morizet, D., & Bally‐Cuif, L. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00525 · Cited by 60 · see citing works
Xie, X., Laks, D. R., Sun, D., Poran, A., Laughney, A. M., Wang, Z., Sam, J., Belenguer, G., Fariñas, I., Elemento, O., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2014389117 · Cited by 59 · see citing works
Swartzlander, D. B., Propson, N. E., Roy, E. R., Saito, T., Saido, T. C., Wang, B., & Zheng, H. (2018) · JCI Insight · doi:10.1172/jci.insight.121109 · Cited by 54 · see citing works
Post-stroke Neurogenesis: Friend or Foe?
Cuartero, M. I., García‐Culebras, A., Torres‐López, C., Medina, V., Fraga, E., Vázquez‐Reyes, S., Jareño-Flores, T., García-Segura, J. M., Lizasoaín, I., & Moro, M. Á. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.657846 · Cited by 59 · see citing works
Li, F., Ducker, M., Sun, B., Szele, F. G., & Czernuszka, J. T. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.05.042 · Cited by 57 · see citing works
Single-Cell Applications of Next-Generation SequencingPreprint
Anaparthy, N., Ho, Y.-J., Martelotto, L. G., Hammell, M., & Hicks, J. B. (2019) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a026898 · Cited by 55 · see citing works
Shi, Z., Geng, Y., Liu, J., Zhang, H., Zhou, L., Lin, Q., Yu, J., Zhang, K., Liu, J., Gao, X., et al. (2017) · Protein & Cell · doi:10.1007/s13238-017-0450-2 · Cited by 51 · see citing works
Effects of Radiation Therapy on Neural Stem Cells
Michaelidesova, A. J., Konířová, J., Bartůněk, P., & Zı́ková, M. (2019) · Genes · doi:10.3390/genes10090640 · Cited by 51 · see citing works
Neural stem cell metabolism revisited: a critical role for mitochondria
Scandella, V., Petrelli, F., Moore, D. L., Braun, S. M. G., & Knobloch, M. (2023) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2023.05.008 · Cited by 53 · see citing works
Bast, L., Calzolari, F., Strasser, M. K., Hasenauer, J., Theis, F. J., Ninkovic, J., & Marr, C. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.11.088 · Cited by 50 · see citing works
Diversity of Adult Neural Stem and Progenitor Cells in Physiology and Disease
Finkel, Z., Esteban, F., Rodriguez, B., Fu, T., Ai, X., & Cai, L. (2021) · Cells · doi:10.3390/cells10082045 · Cited by 46 · see citing works
Tracking cell-type-specific temporal dynamics in human and mouse brains
Lu, Z., Zhang, M., Lee, J., Sziraki, A., Anderson, S., Zhang, Z., Xu, Z., Jiang, W., Ge, S., Nelson, P. T., et al. (2023) · Cell · doi:10.1016/j.cell.2023.08.042 · Cited by 50 · see citing works
A descriptive marker gene approach to single-cell pseudotime inference
Campbell, K. R., & Yau, C. (2018) · Bioinformatics · doi:10.1093/bioinformatics/bty498 · Cited by 47 · see citing works
Weissleder, C., North, H. F., Bitar, M., Fullerton, J. M., Sager, R., Barry, G., Piper, M., Halliday, G. M., Webster, M. J., & Weickert, C. S. (2021) · Molecular Psychiatry · doi:10.1038/s41380-021-01149-3 · Cited by 46 · see citing works
Injury-specific factors in the cerebrospinal fluid regulate astrocyte plasticity in the human brain
Sirko, S., Schichor, C., Vecchia, P. D., Metzger, F., Sonsalla, G., Simon, T., Bürkle, M., Kalpazidou, S., Ninkovic, J., Masserdotti, G., et al. (2023) · Nature Medicine · doi:10.1038/s41591-023-02644-6 · Cited by 45 · see citing works
Alzheimer’s disease, neural stem cells and neurogenesis: cellular phase at single-cell level
Kızıl, Ç., Coşacak, M. İ., & Bhattarai, P. (2019) · Neural Regeneration Research · doi:10.4103/1673-5374.268896 · Cited by 45 · see citing works
Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma
O’Connor, S. A., Feldman, H. M., Arora, S., Hoellerbauer, P., Toledo, C. M., Corrin, P. D., Carter, L. M., Kufeld, M., Bolouri, H., Basom, R., et al. (2021) · Molecular Systems Biology · doi:10.15252/msb.20209522 · Cited by 44 · see citing works
Shu, M., Xue, X., Nie, H., Wu, X., Sun, M., Qiao, L., Li, X., Bai, X., Xiao, Z., Zhao, Y., et al. (2021) · Science China Life Sciences · doi:10.1007/s11427-020-1930-0 · Cited by 44 · see citing works
MicroRNAs Engage in Complex Circuits Regulating Adult Neurogenesis
Stappert, L., Klaus, F., & Brüstle, O. (2018) · Frontiers in Neuroscience · doi:10.3389/fnins.2018.00707 · Cited by 42 · see citing works
scAIDE: clustering of large-scale single-cell RNA-seq data reveals putative and rare cell types
Xie, K., Huang, Y., Zeng, F., Liu, Z., & Chen, T. (2020) · NAR Genomics and Bioinformatics · doi:10.1093/nargab/lqaa082 · Cited by 38 · see citing works
The Subventricular Zone, a Hideout for Adult and Pediatric High-Grade Glioma Stem Cells
Lombard, A., Digregorio, M., Delcamp, C., Rogister, B., Piette, C., & Coppieters, N. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2020.614930 · Cited by 39 · see citing works
Transcriptional and epigenetic mechanisms underlying astrocyte identity
Pavlou, M. A. S., Grandbarbe, L., Buckley, N. J., Niclou, S. P., & Michelucci, A. (2018) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2018.12.007 · Cited by 37 · see citing works
Li, H., Natarajan, A., Ezike, J., Barrasa, M. I., Le, Y., Feder, Z. A., Yang, H., Ma, C., Markoulaki, S., & Lodish, H. F. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.01.026 · Cited by 38 · see citing works
Zhou, B., Yang, B., Liu, Q., Jin, L., Shao, Y., Yuan, T., Zhang, Y., Wang, C., Shi, Z., Li, X., et al. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c13373 · Cited by 36 · see citing works
Hamed, A. A., Kunz, D. J., El-Hamamy, I., Trinh, Q. M., Subedar, O. D., Richards, L. M., Foltz, W. D., Bullivant, G., Ware, M. A., Vladoiu, M. C., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-31408-y · Cited by 37 · see citing works
Wang, J., Jenjaroenpun, P., Bhinge, A., Angarica, V. E., del Sol, A., Nookaew, I., Kuznetsov, V. A., & Stanton, L. W. (2017) · Genome Research · doi:10.1101/gr.223313.117 · Cited by 35 · see citing works
Donega, V., Burm, S. M., van Strien, M. E., van Bodegraven, E. J., Paliukhovich, I., Geut, H., van de Berg, W. D. J., Li, K. W., Smit, A. B., Başak, O., et al. (2019) · Acta Neuropathologica Communications · doi:10.1186/s40478-019-0736-0 · Cited by 36 · see citing works
GemC1 is a critical switch for neural stem cell generation in the postnatal brain
Lalioti, M., Kaplani, K., Lokka, G., Georgomanolis, T., Kyrousi, C., Dong, W., Dunbar, A., Parlapani, E., Damianidou, E., Spassky, N., et al. (2019) · Glia · doi:10.1002/glia.23690 · Cited by 34 · see citing works
Irx3 and Irx5 - Novel Regulatory Factors of Postnatal Hypothalamic Neurogenesis
Dou, Z., Son, J. E., & Hui, C. (2021) · Frontiers in Neuroscience · doi:10.3389/fnins.2021.763856 · Cited by 35 · see citing works
North, H. F., Weissleder, C., Fullerton, J. M., Webster, M. J., & Weickert, C. S. (2022) · Schizophrenia Research · doi:10.1016/j.schres.2022.08.025 · Cited by 34 · see citing works
Yeo, R. W., Zhou, O. Y., Zhong, B. L., Sun, E., Negredo, P. N., Nair, S., Sharmin, M., Ruetz, T. J., Wilson, M., Kundaje, A. B., et al. (2023) · Nature Aging · doi:10.1038/s43587-023-00449-3 · Cited by 37 · see citing works
Influence of glioblastoma contact with the subventricular zone on survival and recurrence patterns
Comas, S., Luguera, E., Molero, J., Balañá, C., Estival, A., Castañer, S., Carrato, C., Hostalot, C., Teixidor, P., & Villà, S. (2020) · Clinical & Translational Oncology · doi:10.1007/s12094-020-02448-x · Cited by 33 · see citing works
Nath, S., Santamaría, J. C. M., Chu, Y., Choi, J. S., Conforti, P., Lin, J.-D., Sankowski, R., Amann, L., Galanis, C., Wu, K., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-53217-1 · Cited by 31 · see citing works
Cochard, L. M., Levros, L.-C., Joppé, S. E., Pratesi, F., Aumont, A., & Fernandes, K. J. L. (2021) · Frontiers in Neuroscience · doi:10.3389/fnins.2021.621076 · Cited by 31 · see citing works
Borrett, M. J., Innes, B. T., Tahmasian, N., Bader, G. D., Kaplan, D. R., & Miller, F. D. (2022) · eNeuro · doi:10.1523/eneuro.0271-21.2021 · Cited by 31 · see citing works
Nieto-González, J. L., Gómez‐Sánchez, L., Mavillard, F., Linares‐Clemente, P., Rivero, M., Valenzuela-Villatoro, M., Muñoz‐Bravo, J. L., Pardal, R., & Fernández‐Chacón, R. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1817183116 · Cited by 30 · see citing works
Mieth, B., Hockley, J. R. F., Görnitz, N., Vidovic, M. M.-C., Müller, K., Gutteridge, A., & Ziemek, D. (2019) · Scientific Reports · doi:10.1038/s41598-019-56911-z · Cited by 30 · see citing works
The circadian clock in adult neural stem cell maintenance
Draijer, S., Chaves, I., & Hoekman, M. F. M. (2018) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2018.05.007 · Cited by 30 · see citing works
Functional rejuvenation of aged neural stem cells by Plagl2 and anti-Dyrk1a activityPreprint
Kaise, T., Fukui, M., Sueda, R., Piao, W., Yamada, M., Kobayashi, T., Imayoshi, I., & Kageyama, R. (2021) · Genes & Development · doi:10.1101/gad.349000.121 · Cited by 30 · see citing works
Design principles of cell-state-specific enhancers in hematopoiesis
Frömel, R., Rühle, J., Bernal Martinez, A., Szu-Tu, C., Pacheco Pastor, F., Martinez-Corral, R., & Velten, L. (2025) · Cell · doi:10.1016/j.cell.2025.04.017 · Cited by 29 · see citing works
Single cell transcriptome sequencing: A new approach for the study of mammalian sex determination
Stévant, I., & Nef, S. (2018) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2018.01.013 · Cited by 28 · see citing works
- Authors
- Dulken, B. W., Leeman, D. S., Boutet, S. C., Hebestreit, K., & Brunet, A.
- Affiliations
- Institute for Stem Cell Biology and Regenerative Medicine (US)
- Stanford Medicine (US)
- Standard BioTools (United States) (US)
- Stanford University (US)
- Corresponding author
- Brunet, Anne
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2017-01
- Volume
- 18
- Issue
- 3
- Pages
- 777-790
- ISSN
- 2211-1247 (ISSN-L)
- PMC
- PMC5269583
- OpenAlex
- W2578839378
- Cited by
- 334
- References
- 46
- Citations by year
- 2026: 15 · 2025: 33 · 2024: 34 · 2023: 35 · 2022: 37 · 2021: 44 · 2020: 32 · 2019: 50 · 2018: 40 · 2017: 13 · 2016: 1
- FWCI
- 15.61
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- NIH Training Grant (T32 GM7365)
- Stanford MSTP program
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- adult neural stem cells, transcriptional dynamics, stem cell activation, stem cell differentiation, cell surface markers, neurogenic niche, single-cell transcriptomics
- MeSH terms
- Sodium-Potassium-Exchanging ATPase, Algorithms, Animals, Astrocytes, Cell Differentiation, RNA, Transcription Factors, Sequence Analysis, RNA, Connexin 43, Homeodomain Proteins, Cell Lineage, Ki-67 Antigen, Principal Component Analysis, Mice, Neurogenesis, Neural Stem Cells, Single-Cell Analysis, Transcriptome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
