Industrial optimization of fucoidan extraction from Sargassum sp. and its potential antioxidant and emulsifying activities
Abstract
There is a growing demand to find an effective extraction process of sulfated polysaccharides from brown algae to conserve its structure and biological activity. Fucoidan was recovered from Sargassum sp. using a hot buffer extraction process. Box–Benhken experimental design was evaluated to study different conditions of temperature, pH and buffer: alga ratio on fucoidan yield and its sulfate content. By solving the regression equations and analyzing 3-D plots, the optimum conditions were at extraction temperature 60 °C, pH 4.0, and ratio of buffer: alga 10.0 mL/g. Under these conditions, the experimental fucoidan yield, and sulfate content were 19 and 47.6% (w/w), respectively, which were in good agreement with the predicted values. The use of hot buffer extraction was efficient to obtain a high fucoidan yield with maintaining high sulfate contents. Fucoidan preparations showed variations in antioxidant properties using various antioxidant assays. Increasing reducing antioxidant activity and hydroxyl radical scavenging of fucoidan extracts was attributed to increasing total sugars, fucose, and uronic acids. The data obtained suggested that the sulfate groups might act as reductones rather than radical scavengers to contribute to the antioxidant activity of fucoidan. Additionally, proteinaceous and phenolic compounds co-extracted with crude fucoidan contributed to its antioxidant potential. Crude fucoidan demonstrated good emulsion stabilizing capacities, especially with cedar wood oil and xylene. These results suggest the use of the crude fucoidan as a good alternative to many synthetic polymers, as well as other natural polysaccharides, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.
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Fucoidan fractions with high total sugars, fucose and uronic acids show increased radical scavenging activity, while highly sulphated molecules show high FRAP antioxidant activity.
“Fucoidan fractions containing high levels of total sugars, fucose, and uronic acids showed increased radical scavenging activity while molecules with high degree of sulphation showed high FRAP antioxidant activity”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Hifney et al., 2016
Studies optimizing macroalgal polysaccharide extraction mainly used Box-Behnken and central composite designs to analyse at least three factors.
“Studies on the optimization of one or more steps of the extraction processes of polysaccharides from macroalgae mainly used Box-Behnken and central composite designs to analyse at least 3 or more factors affecting the extraction process”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Hifney et al., 2016
RSM was applied to optimize yield and degree of sulphation of fucoidan from Sargassum sp. using McIlvaine buffer at different temperatures, pH and macroalgae-to-buffer ratios.
“The same RSM design was applied most recently to optimize the yield and degree of sulphation of fucoidan from dried-milled Sargassum sp. using McIlvaine buffer solution at different temperatures, pH and macroalgae to buffer ratios”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Hifney et al., 2016
In vitro antioxidant activity of polysaccharides is mainly determined by DPPH, ABTS and FRAP assays.
“The in vitro antioxidant activity of polysaccharides have been determined mainly by 1,1diphenyl-2-picryl hydrazil (DPPH) radical scavenging, ABTS radical scavenging and ferric reducing antioxidant power (FRAP) assays amongst others”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Hifney et al., 2016
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- Authors
- Hifney, A. F., Fawzy, M. A., Abdel-Gawad, K. M., & Gomaa, M.
- Affiliations
- Assiut University (EG)
- Corresponding author
- Gomaa, Mohamed
- Venue
- Food Hydrocolloids
- Publisher
- Elsevier BV
- Published
- 2016-03
- Volume
- 54
- Pages
- 77-88
- ISSN
- 0268-005X (ISSN-L)
- OpenAlex
- W1738299799
- Semantic Scholar
- 29ca76e3360b1c83fc112175a281d4e6d065d579
- Cited by
- 216
- References
- 54
- Citations by year
- 2026: 19 · 2025: 18 · 2024: 35 · 2023: 22 · 2022: 24 · 2021: 19 · 2020: 25 · 2019: 19 · 2018: 14 · 2017: 14 · 2016: 7
- FWCI
- 18.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Hydrocolloids (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Keywords
- fucoidan, Sargassum, sulfated polysaccharides, extraction optimization, Box-Behnken design, antioxidant activity, emulsifying activity, hydroxyl radical scavenging, uronic acid, fucose, xylene, emulsion stabilization, brown algae
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