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Cited by 258

The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.

Two-dimensional gel electrophoresis in proteomics: A tutorial

Open access
Rabilloud, Thierry, Lelong, Cécile·2011·Journal of Proteomics
DOI 10.1016/j.jprot.2011.05.040
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Abstract

Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the process, from sample preparation to in-gel detection of proteins, commenting the constraints and caveats of the technique. Then the limitations and positive features of two-dimensional electrophoresis are discussed (e.g. its unique ability to separate complete proteins and its easy interfacing with immunoblotting techniques), so that the optimal type of applications of this technique in current and future proteomics can be perceived. This is illustrated by a detailed example taken from the literature and commented in detail. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 2).

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Metadata

Authors
Rabilloud, T., & Lelong, C.
Venue
Journal of Proteomics
Publisher
Elsevier BV
Published
2011-09
Volume
74
Issue
10
Pages
1829-1841
ISSN
1874-3919 (ISSN-L)
DOI
10.1016/j.jprot.2011.05.040
OpenAlex
W1985072643
Cited by
258
References
69
FWCI
5.33
Type
journal article
Language
en
Open access
hybrid

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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