Cited by 217Seaweed-derived Bioactive CompoundsPolysaccharides Composition and ApplicationsPolysaccharides and Plant Cell Walls

Structural characterization of laminaran and galactofucan extracted from the brown seaweed Saccharina longicruris

Rioux, Laurie-Eve, Turgeon, Sylvie L., Beaulieu, Martin·2010·Phytochemistry

Abstract

Brown seaweed contains several polysaccharides like laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights <30 kDa. The structure of the galactofucan fractions remained complex after depolymerization, with these also being more sulfated (30-39%) than the crude fractions (13-20%). The crude and RDP fractions contained 3-linked fucopyranose 4-sulfate and 6-linked galactopyranose 3-sulfate moieties, although the galactofucans isolated from M05 and J06 contained less 6-linked galactopyranose 3-sulfate than the A05 and N05 fractions.

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Crossref · 10.1016/j.phytochem.2010.05.021

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Authors
Rioux, L.-E., Turgeon, S. L., & Beaulieu, M.
Affiliations
  • Université Laval (CA)
  • InnoVactiv (Canada) (CA)
Corresponding author
Turgeon, Sylvie L.
Venue
Phytochemistry
Publisher
Elsevier BV
Published
2010-09
Volume
71
Issue
13
Pages
1586-1595
ISSN
0031-9422 (ISSN-L)
OpenAlex
W1970579342
Cited by
216
References
52
Citations by year
2026: 10 · 2025: 8 · 2024: 15 · 2023: 21 · 2022: 12 · 2021: 12 · 2020: 25 · 2019: 17 · 2018: 17 · 2017: 12 · 2016: 12 · 2015: 15 · 2014: 10 · 2013: 13 · 2012: 11
FWCI
14.83
Type
journal article
Language
en
Open access
closed
Locations
  • Phytochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fonds Québécois de la Recherche sur la Nature et les Technologies
  • Natural Sciences and Engineering Research Council of Canada
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Keywords
laminarin, fucoidan, brown seaweed polysaccharides, polysaccharide depolymerization, molecular weight distribution, monosaccharide composition, glycosidic linkage, sulfation, harvest time, polysaccharide structure
MeSH terms
Glucans, Gas Chromatography-Mass Spectrometry, Polysaccharides, Seaweed, Time Factors, Spectroscopy, Fourier Transform Infrared, Spectrometry, Mass, Electrospray Ionization

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