Structural characterization of laminaran and galactofucan extracted from the brown seaweed Saccharina longicruris
Abstract
Brown seaweed contains several polysaccharides like laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights <30 kDa. The structure of the galactofucan fractions remained complex after depolymerization, with these also being more sulfated (30-39%) than the crude fractions (13-20%). The crude and RDP fractions contained 3-linked fucopyranose 4-sulfate and 6-linked galactopyranose 3-sulfate moieties, although the galactofucans isolated from M05 and J06 contained less 6-linked galactopyranose 3-sulfate than the A05 and N05 fractions.
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High molecular weight laminaran (19-27 kDa) was isolated from Eisenia bicyclis.
“High molecular weight laminaran (19–27 kDa) was recently isolated from the brown seaweed Eisenia bicyclis.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [85]
This laminaran and its enzymatic degradation products inhibited colony formation of SK-Mel-28 and colon cancer DLD cells.
“This laminaran and its products of enzymatic degradation inhibited the colony formation of SK-Mel-28 and colon cancer DLD cells.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [85]
This 1,3;1,6-β-d-glucan contained 1,6-linked glucose residues in both branches and main chain, basically in non-reduced ends.
“It was shown that this 1,3;1,6-β-d-glucan contained 1,6-linked glucose residues in both branches and the main chain, basically in the non-reduced ends of the molecules.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [85]
Increase of 1,6-linked glucose residues and decrease of molecular weight improved anticancer effect in this series.
“The increase of the content of 1,6-linked glucose residues and the decrease of the molecular weight improved the anticancer effect in this series of substances [85].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [85]
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- Authors
- Rioux, L.-E., Turgeon, S. L., & Beaulieu, M.
- Affiliations
- Université Laval (CA)
- InnoVactiv (Canada) (CA)
- Corresponding author
- Turgeon, Sylvie L.
- Venue
- Phytochemistry
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 71
- Issue
- 13
- Pages
- 1586-1595
- ISSN
- 0031-9422 (ISSN-L)
- OpenAlex
- W1970579342
- Cited by
- 216
- References
- 52
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 15 · 2023: 21 · 2022: 12 · 2021: 12 · 2020: 25 · 2019: 17 · 2018: 17 · 2017: 12 · 2016: 12 · 2015: 15 · 2014: 10 · 2013: 13 · 2012: 11
- FWCI
- 14.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phytochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fonds Québécois de la Recherche sur la Nature et les Technologies
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- laminarin, fucoidan, brown seaweed polysaccharides, polysaccharide depolymerization, molecular weight distribution, monosaccharide composition, glycosidic linkage, sulfation, harvest time, polysaccharide structure
- MeSH terms
- Glucans, Gas Chromatography-Mass Spectrometry, Polysaccharides, Seaweed, Time Factors, Spectroscopy, Fourier Transform Infrared, Spectrometry, Mass, Electrospray Ionization
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