Intrafascicular electrodes for stimulation and recording from mudpuppy spinal roots
Abstract
This paper presents a technique for stimulating and recording from multiple intact spinal roots in the in vitro mudpuppy (Necturus maculatus) spinal cord-forearm preparation using fine wire electrodes, a modified intrafascicular electrode. We found that multiple spinal roots of the preparation could be implanted with these modified electrodes for independent stimulation or recording of the roots without inducing mechanical vibrations, disrupting conduction, or obscuring the view of or access to the spinal cord. Recording and stimulation performance using these electrodes was compared with results obtained using conventional hook electrodes. We found that intrafascicular electrodes were more efficient than hook electrodes for stimulating nerve fibers, being able to produce equivalent levels of activation using stimulation levels that were an order of magnitude smaller. Compound action potential signals recorded from electrodes implanted in the spinal roots were found to be larger than those from hook electrodes placed around the corresponding spinal nerve, showing that intrafascicular electrodes are more efficient at recording activity in the nerve. Moreover, it was possible to record evoked activity from cutaneous mechanoreceptors, even though the signal to noise ratio was low. Rough estimates of the conduction velocities for the fastest components in the compound action potentials were calculated and found to be around 17.5 m/s for both dorsal and ventral roots.
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Comparatively smaller stimulus intensities can be used with intrafascicular electrodes to achieve equivalent levels to those of extraneural electrodes.
“Comparatively smaller stimulus intensities can be used to achieve equivalent levels to those of extraneural electrodes (Yoshida et al., 2000) .”
From A critical review of interfaces with the peripheral nervous system for the control of neuroprostheses and hybrid bionic systems (2005) · cites this paper as Yoshida et al., 2000
Activity from cutaneous receptors in response to stimuli has been recorded in several studies, but the activity was small and difficult to distinguish from background noise.
“Activity arising from cutaneous receptors in response to stimuli has been recorded in several studies, although activity was found to be small and difficult to distinguish from the background noise in the recording (Malmstrom et al., 1998; Yoshida et al., 2000) .”
From A critical review of interfaces with the peripheral nervous system for the control of neuroprostheses and hybrid bionic systems (2005) · cites this paper as Yoshida et al., 2000
Muscle recruitment curves as function of current amplitude were typical for the stimulation strategies used.
“Muscle recruitment curves as function of current amplitude were typical for the stimulation strategies used (Grill and Mortimer 1996b, Yoshida et al 2000, Navarro et al 2001, 2007, Polasek et al 2007).”
From Comparative analysis of transverse intrafascicular multichannel, longitudinal intrafascicular and multipolar cuff electrodes for the selective stimulation of nerve fascicles (2011) · cites this paper as Yoshida et al 2000
To achieve a selective interfacing with several nerve fascicles, a corresponding number of LIFEs needs to be implanted, whereas only one TIME may provide the same results.
“though the LIFE has a high degree of selectivity (Yoshida et al 2000), to achieve a selective interfacing with several nerve fascicles a corresponding number of LIFEs needs to be implanted, whereas only one TIME may provide the same results”
From Comparative analysis of transverse intrafascicular multichannel, longitudinal intrafascicular and multipolar cuff electrodes for the selective stimulation of nerve fascicles (2011) · cites this paper as Yoshida et al 2000
The currents applied to TIME, LIFE and cuff devices remained within the range of safety.
“The currents applied to TIME, LIFE and cuff devices remained within the range of safety (Yoshida et al 2000, Navarro et al 2001, Boretius et al 2010).”
From Comparative analysis of transverse intrafascicular multichannel, longitudinal intrafascicular and multipolar cuff electrodes for the selective stimulation of nerve fascicles (2011) · cites this paper as Yoshida et al 2000
Longitudinal intra-fascicular electrodes can selectively stimulate nerve fibers, enabling activation of separate axonal subsets within a single fascicle and induction of graded responses.
“They are also able to selectively stimulate nerve fibers, even enabling the activation of separate axonal subsets within a single fascicle [88], and the induction of graded responses [89].”
From Interfaces with the peripheral nervous system for the control of a neuroprosthetic limb: a review (2020) · cites this paper as [89]
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- Authors
- Yoshida, K., Jovanović, K., & Stein, R. B.
- Affiliations
- Aalborg University (DK)
- University of Alberta (CA)
- Corresponding author
- Yoshida, Ken
- Venue
- Journal of Neuroscience Methods
- Publisher
- Elsevier BV
- Published
- 2000-03
- Volume
- 96
- Issue
- 1
- Pages
- 47-55
- ISSN
- 0165-0270 (ISSN-L)
- OpenAlex
- W1984905989
- Cited by
- 49
- References
- 19
- Citations by year
- 2024: 4 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 4 · 2013: 1 · 2012: 1
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroscience Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Danmarks Grundforskningsfond
- Fondation pour la Recherche Médicale
- Medical Research Council
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Neuroscience › Cognitive Neuroscience › Undergraduate Neuroscience Education and Research
- Physical Sciences › Engineering › Biomedical Engineering › Muscle activation and electromyography studies
- Keywords
- intrafascicular electrodes, mudpuppy, spinal nerve roots, fine-wire electrodes, neurostimulation, compound action potential, cutaneous mechanoreceptors, evoked activity, nerve conduction velocity
- MeSH terms
- Action Potentials, Animals, Electric Stimulation, Electrodes, Implanted, Electromyography, Evoked Potentials, Somatosensory, Forelimb, Necturus, Nerve Crush, Neural Conduction, Physical Stimulation, Spinal Nerve Roots
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