Cited by 98Gut microbiota and healthAdipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic Diseases

Gut microbiota modulate the metabolism of brown adipose tissue in mice

Mestdagh, Renaud, Dumas, Marc-Emmanuel, Rezzi, Serge, Kochhar, Sunil, Holmes, Elaine, Claus, Sandrine P., Nicholson, Jeremy K.·2012·Journal of Proteome Research

Abstract

A two by two experimental study has been designed to determine the effect of gut microbiota on energy metabolism in mouse models. The metabolic phenotype of germ-free (GF, n = 20) and conventional (n = 20) mice was characterized using a NMR spectroscopy-based metabolic profiling approach, with a focus on sexual dimorphism (20 males, 20 females) and energy metabolism in urine, plasma, liver, and brown adipose tissue (BAT). Physiological data of age-matched GF and conventional mice showed that male animals had a higher weight than females in both groups. In addition, conventional males had a significantly higher total body fat content (TBFC) compared to conventional females, whereas this sexual dimorphism disappeared in GF animals (i.e., male GF mice had a TBFC similar to those of conventional and GF females). Profiling of BAT hydrophilic extracts revealed that sexual dimorphism in normal mice was absent in GF animals, which also displayed lower BAT lactate levels and higher levels of (D)-3-hydroxybutyrate in liver, plasma, and BAT, together with lower circulating levels of VLDL. These data indicate that the gut microbiota modulate the lipid metabolism in BAT, as the absence of gut microbiota stimulated both hepatic and BAT lipolysis while inhibiting lipogenesis. We also demonstrated that (1)H NMR metabolic profiles of BAT were excellent predictors of BW and TBFC, indicating the potential of BAT to fight against obesity.

Integrity signals

Registry

Crossref · 10.1021/pr200938v

Cites

60 of 60 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Animal experiments have provided solid proof that the sexual dimorphism in total body fat content seen in rodents (males exhibiting higher fat content than females) fades away in germ free animals, suggesting a role for the gut microbiota.

    “Animal experiments have provided solid proof for this phenomenon, as the sexual dimorphism in total body fat content seen in rodents (males exhibiting higher fat content than females) has shown a fade away in germ free animals, thus suggesting a role for the gut microbiota [47].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Mestdagh, R., Dumas, M.-E., Rezzi, S., Kochhar, S., Holmes, E., Claus, S. P., & Nicholson, J. K.
Affiliations
  • Imperial College London (GB)
  • Nestlé (Switzerland) (CH)
Corresponding author
  • Claus, Sandrine P.
  • Nicholson, Jeremy K.
Venue
Journal of Proteome Research
Published
2011-11-21
Volume
11
Issue
2
Pages
620-630
ISSN
  • 1535-3893 (ISSN-L)
  • 1535-3907
OpenAlex
W2069699035
Cited by
97
References
60
Citations by year
2026: 2 · 2025: 7 · 2024: 5 · 2023: 5 · 2022: 5 · 2021: 10 · 2020: 5 · 2019: 7 · 2018: 5 · 2017: 8 · 2016: 7 · 2015: 7 · 2014: 4 · 2013: 11 · 2012: 9
FWCI
3.11
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Proteome Research (publishedVersion)
  • PubMed (publishedVersion)
  • CentAUR (University of Reading) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
gut microbiota, brown adipose tissue, energy metabolism, germ-free mice, sexual dimorphism, lipid metabolism, lipolysis, lipogenesis, beta-hydroxybutyrate, lactate, VLDL, obesity, NMR metabolomics, body weight
MeSH terms
Adipose Tissue, Animals, Body Weight, Adipose Tissue, Brown, Female, Germ-Free Life, Liver, Male, Sex Characteristics, Symbiosis, Urine, Discriminant Analysis, Least-Squares Analysis, Nuclear Magnetic Resonance, Biomolecular, 3-Hydroxybutyric Acid, Gastrointestinal Tract, Lipid Metabolism, Mice, Metagenome, Metabolome

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review