Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice
Open accessAbstract
Recent data suggest that the gut microbiota plays a significant role in fat accumulation. However, it is not clear whether gut microbiota is involved in the pathophysiology of type 2 diabetes. To assess this issue, we modulated gut microbiota via antibiotics administration in two different mouse models with insulin resistance. Results from dose‐determination studies showed that a combination of norfloxacin and ampicillin, at a dose of 1g/L, maximally suppressed the numbers of cecal aerobic and anaerobic bacteria in ob/ob mice. After a 2‐wk intervention with the antibiotic combination, both ob/ob and diet‐induced obese and insulin‐resistant mice showed a significant improvement in fasting glycemia and oral glucose tolerance. The improved glycemic control was independent of food intake or adiposity because pair‐fed ob/ob mice were as glucose intolerant as the control ob/ob mice. Reduced liver triglycerides and increased liver glycogen correlated with improved glucose tolerance in the treated mice. Concomitant reduction of plasma lipopolysaccharides and increase of adiponectin further supported the antidiabetic effects of the antibiotic treatment in ob/ob mice. In summary, modulation of gut microbiota ameliorated glucose tolerance of mice by altering the expression of hepatic and intestinal genes involved in inflammation and metabolism, and by changing the hormonal, inflammatory, and metabolic status of the host.—Membrez, M., Blancher, F., Jaquet, M., Bibiloni, R., Cani, P. D., Burcelin, R. G., Corthesy, I., Macé, K., Chou, C. J. Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice. FASEB J. 22, 2416–2426 (2008)
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Murine data show that nonabsorbable antibiotics and norfloxacin improve alcoholic liver disease and insulin resistance in NAFLD.
“As data from murine studies show that non-absorbable antibiotics and norfloxacin improve ALD48,116 and insulin resistance in NAFLD117, they might be particularly attractive for HCC prevention in these patient groups.”
From The gut microbiome and liver cancer: mechanisms and clinical translation (2017) · cites this paper as [117]
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- Authors
- Membrez, M., Blancher, F., Jaquet, M., Bibiloni, R., Cani, P. D., Burcelin, R. G., Corthesy, I., Macé, K., & Chou, C. J.
- Affiliations
- Nestlé (Switzerland) (CH)
- UCLouvain (BE)
- Institute of Molecular Medicine Rangueil (12 MR) INSERM U858 IFR31 Toulouse France
- Nestlé Research Center Lausanne Switzerland
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2008-03-07
- Volume
- 22
- Issue
- 7
- Pages
- 2416-2426
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2110569929
- Cited by
- 493
- References
- 44
- Citations by year
- 2026: 8 · 2025: 7 · 2024: 16 · 2023: 12 · 2022: 13 · 2021: 24 · 2020: 26 · 2019: 31 · 2018: 35 · 2017: 29 · 2016: 45 · 2015: 38 · 2014: 32 · 2013: 50 · 2012: 36
- FWCI
- 12.36
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Digital Access to Libraries (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- gut microbiota, norfloxacin, ampicillin, glucose tolerance, type 2 diabetes, insulin resistance, obesity, ob/ob mice, diet-induced obesity, fasting blood glucose, oral glucose tolerance, hepatic triglycerides, liver glycogen, lipopolysaccharide, adiponectin, inflammation, hepatic gene expression, intestinal gene expression
- MeSH terms
- Ampicillin, Animals, Anti-Bacterial Agents, Bacteroides, Bifidobacterium, Diabetes Mellitus, Type 2, Enterobacteriaceae, Lactobacillus, Mice, Obese, Microbial Sensitivity Tests, Norfloxacin, Obesity, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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