Cited by 493Gut microbiota and healthAdipokines, Inflammation, and Metabolic DiseasesOral microbiology and periodontitis research

Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice

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Membrez, Mathieu, Blancher, Florence, Jaquet, Muriel, Bibiloni, Rodrigo, Cani, Patrice D., Burcelin, Rèmy G., Corthesy, Irene, Macé, Katherine +1 more·2008·The FASEB Journal

Abstract

Recent data suggest that the gut microbiota plays a significant role in fat accumulation. However, it is not clear whether gut microbiota is involved in the pathophysiology of type 2 diabetes. To assess this issue, we modulated gut microbiota via antibiotics administration in two different mouse models with insulin resistance. Results from dose‐determination studies showed that a combination of norfloxacin and ampicillin, at a dose of 1g/L, maximally suppressed the numbers of cecal aerobic and anaerobic bacteria in ob/ob mice. After a 2‐wk intervention with the antibiotic combination, both ob/ob and diet‐induced obese and insulin‐resistant mice showed a significant improvement in fasting glycemia and oral glucose tolerance. The improved glycemic control was independent of food intake or adiposity because pair‐fed ob/ob mice were as glucose intolerant as the control ob/ob mice. Reduced liver triglycerides and increased liver glycogen correlated with improved glucose tolerance in the treated mice. Concomitant reduction of plasma lipopolysaccharides and increase of adiponectin further supported the antidiabetic effects of the antibiotic treatment in ob/ob mice. In summary, modulation of gut microbiota ameliorated glucose tolerance of mice by altering the expression of hepatic and intestinal genes involved in inflammation and metabolism, and by changing the hormonal, inflammatory, and metabolic status of the host.—Membrez, M., Blancher, F., Jaquet, M., Bibiloni, R., Cani, P. D., Burcelin, R. G., Corthesy, I., Macé, K., Chou, C. J. Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice. FASEB J. 22, 2416–2426 (2008)

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Crossref · 10.1096/fj.07-102723

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  • Murine data show that nonabsorbable antibiotics and norfloxacin improve alcoholic liver disease and insulin resistance in NAFLD.

    “As data from murine studies show that non-absorbable antibiotics and norfloxacin improve ALD48,116 and insulin resistance in NAFLD117, they might be particularly attractive for HCC prevention in these patient groups.”

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Authors
Membrez, M., Blancher, F., Jaquet, M., Bibiloni, R., Cani, P. D., Burcelin, R. G., Corthesy, I., Macé, K., & Chou, C. J.
Affiliations
  • Nestlé (Switzerland) (CH)
  • UCLouvain (BE)
  • Institute of Molecular Medicine Rangueil (12 MR) INSERM U858 IFR31 Toulouse France
  • Nestlé Research Center Lausanne Switzerland
Venue
The FASEB Journal
Publisher
Wiley
Published
2008-03-07
Volume
22
Issue
7
Pages
2416-2426
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2110569929
Cited by
493
References
44
Citations by year
2026: 8 · 2025: 7 · 2024: 16 · 2023: 12 · 2022: 13 · 2021: 24 · 2020: 26 · 2019: 31 · 2018: 35 · 2017: 29 · 2016: 45 · 2015: 38 · 2014: 32 · 2013: 50 · 2012: 36
FWCI
12.36
Type
journal article
Language
en
Open access
Locations
  • The FASEB Journal (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • Digital Access to Libraries (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords
gut microbiota, norfloxacin, ampicillin, glucose tolerance, type 2 diabetes, insulin resistance, obesity, ob/ob mice, diet-induced obesity, fasting blood glucose, oral glucose tolerance, hepatic triglycerides, liver glycogen, lipopolysaccharide, adiponectin, inflammation, hepatic gene expression, intestinal gene expression
MeSH terms
Ampicillin, Animals, Anti-Bacterial Agents, Bacteroides, Bifidobacterium, Diabetes Mellitus, Type 2, Enterobacteriaceae, Lactobacillus, Mice, Obese, Microbial Sensitivity Tests, Norfloxacin, Obesity, Mice

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