Cited by 522T-cell and B-cell ImmunologyImmune responses and vaccinationsImmunotherapy and Immune Responses

Origin and differentiation of human memory CD8 T cells after vaccination

Open access
Akondy, Rama S., Fitch, Mark, Edupuganti, Srilatha, Yang, Shu, Kissick, Haydn T., Li, Kelvin W., Youngblood, Ben A., Abdelsamed, Hossam A. +16 more·2017·Nature

Abstract

The differentiation of human memory CD8 T cells is not well understood. Here we address this issue using the live yellow fever virus (YFV) vaccine, which induces long-term immunity in humans. We used in vivo deuterium labelling to mark CD8 T cells that proliferated in response to the virus and then assessed cellular turnover and longevity by quantifying deuterium dilution kinetics in YFV-specific CD8 T cells using mass spectrometry. This longitudinal analysis showed that the memory pool originates from CD8 T cells that divided extensively during the first two weeks after infection and is maintained by quiescent cells that divide less than once every year (doubling time of over 450 days). Although these long-lived YFV-specific memory CD8 T cells did not express effector molecules, their epigenetic landscape resembled that of effector CD8 T cells. This open chromatin profile at effector genes was maintained in memory CD8 T cells isolated even a decade after vaccination, indicating that these cells retain an epigenetic fingerprint of their effector history and remain poised to respond rapidly upon re-exposure to the pathogen.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/nature24633

Cites

32 of 32 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Akondy, R. S., Fitch, M., Edupuganti, S., Yang, S., Kissick, H. T., Li, K. W., Youngblood, B. A., Abdelsamed, H. A., McGuire, D. J., Cohen, K. W., Alexe, G., Nagar, S., McCausland, M. M., Gupta, S., Tata, P., Haining, W. N., McElrath, M. J., Zhang, D., Hu, B., Greenleaf, W. J., Goronzy, J. J., Mulligan, M. J., Hellerstein, M., & Ahmed, R.
Affiliations
  • Emory University (US)
  • University of California, Berkeley (US)
  • Central South University (CN)
  • KineMed (United States) (US)
  • St. Jude Children's Research Hospital (US)
  • Fred Hutch Cancer Center (US)
  • Broad Institute (US)
  • Strand Life Sciences (India) (IN)
  • Stanford Medicine (US)
Corresponding author
  • Hellerstein, Marc
  • Ahmed, Rafi
Venue
Nature
Published
2017-12-13
Volume
552
Issue
7685
Pages
362-367
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2772063315
Cited by
517
References
32
Citations by year
2026: 36 · 2025: 49 · 2024: 47 · 2023: 57 · 2022: 72 · 2021: 81 · 2020: 71 · 2019: 55 · 2018: 47 · 2017: 2
FWCI
21.74
Type
journal article
Language
en
Open access
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • National Institutes of Health (R01-AI43866-07)
  • National Institute of Allergy and Infectious Diseases (R01AI043866, R01AI129191, UM1AI068618, UM1AI069481, U19AI057266)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (T32AR050942)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
memory CD8+ T cells, yellow fever vaccine, T cell differentiation, T cell quiescence, cell turnover, deuterium labeling, epigenetic memory, open chromatin, DNA methylation, effector T cells, naive T cells, T cell proliferation
MeSH terms
Animals, Cell Differentiation, Chromatin, Deuterium, Half-Life, Humans, Immunologic Memory, Radioisotope Dilution Technique, Transcription, Genetic, Yellow Fever, Yellow fever virus, CD8-Positive T-Lymphocytes, Lymphocyte Count, DNA Methylation, Gene Expression Profiling, Yellow Fever Vaccine, Epigenesis, Genetic, Cell Proliferation, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review