Biodegradable scaffold with built-in vasculature for organ-on-a-chip engineering and direct surgical anastomosis
Open accessAbstract
We report the fabrication of a scaffold (hereafter referred to as AngioChip) that supports the assembly of parenchymal cells on a mechanically tunable matrix surrounding a perfusable, branched, three-dimensional microchannel network coated with endothelial cells. The design of AngioChip decouples the material choices for the engineered vessel network and for cell seeding in the parenchyma, enabling extensive remodelling while maintaining an open-vessel lumen. The incorporation of nanopores and micro-holes in the vessel walls enhances permeability, and permits intercellular crosstalk and extravasation of monocytes and endothelial cells on biomolecular stimulation. We also show that vascularized hepatic tissues and cardiac tissues engineered by using AngioChips process clinically relevant drugs delivered through the vasculature, and that millimetre-thick cardiac tissues can be engineered in a scalable manner. Moreover, we demonstrate that AngioChip cardiac tissues implanted with direct surgical anastomosis to the femoral vessels of rat hindlimbs establish immediate blood perfusion.
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The authors' team developed AngioChip, a multidimensional scaffold with a built-in vascular network using 3D stamping; long-term gravity-driven perfusion improved parenchymal cell viability and enabled vascular sprouting through scaffold micro-holes.
“our team has developed a multidimensional scaffold with a built-in vascular network using a 3D stamping technique (AngioChip).”
From Cardiovascular disease models: A game changing paradigm in drug discovery and screening (2019) · cites this paper as [266]
In AngioChip, parenchymal cells showed physiological drug responses through the vasculature: epinephrine increased spontaneous beating rate, and engineered tissue metabolized terfenadine to fexofenadine.
“engineered cardiac tissue perfused with epinephrine increased the spontaneous beating rate, and engineered metabolised terfenadine to fexofenadine [266].”
From Cardiovascular disease models: A game changing paradigm in drug discovery and screening (2019) · cites this paper as [266]
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Bioengineering approaches to treat the failing heart: from cell biology to 3D printing
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Understanding Immune‐Driven Brain Aging by Human Brain Organoid Microphysiological Analysis Platform
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- Authors
- Zhang, B., Montgomery, M., Chamberlain, M. D., Ogawa, S., Korolj, A., Pahnke, A., Wells, L. A., Massé, S., Kim, J., Reis, L., Momen, A., Nunes, S. S., Wheeler, A. R., Nanthakumar, K., Keller, G., Sefton, M. V., & Radisic, M.
- Affiliations
- University of Toronto (CA)
- Toronto General Hospital (CA)
- University Health Network (CA)
- Corresponding author
- Radisic, Milica
- ORCID
- Venue
- Nature Materials
- Published
- 2016-03-07
- Volume
- 15
- Issue
- 6
- Pages
- 669-678
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- PMC
- PMC4879054
- OpenAlex
- W2293502352
- Cited by
- 593
- References
- 63
- Citations by year
- 2026: 30 · 2025: 39 · 2024: 60 · 2023: 54 · 2022: 55 · 2021: 86 · 2020: 65 · 2019: 64 · 2018: 55 · 2017: 63 · 2016: 22
- FWCI
- 32.73
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (2r01hl076485-16)
- National Heart, Lung, and Blood Institute (R01-HL076485)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- biodegradable scaffolds, organ-on-a-chip, vascularized tissue engineering, tunable mechanical properties, nanopores, vascular permeability, monocyte extravasation, engineered heart tissue, surgical anastomosis, blood perfusion, vascular remodeling
- MeSH terms
- Anastomosis, Surgical, Animals, Biocompatible Materials, Femur, Humans, Liver, Monocytes, Myocardium, Rats, Inbred Lew, Porosity, Tissue Engineering, Rats, Tissue Scaffolds, Lab-On-A-Chip Devices, Human Umbilical Vein Endothelial Cells
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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