Cited by 227Gut microbiota and healthOral microbiology and periodontitis researchColorectal Cancer Treatments and Studies

Distinct gut microbiome patterns associate with consensus molecular subtypes of colorectal cancer

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Purcell, Rachel V., Visnovska, Martina, Biggs, Patrick J., Schmeier, Sebastian, Frizelle, Frank A.·2017·Scientific Reports

Published version of a preprint

Abstract

Colorectal cancer (CRC) is a heterogeneous disease and recent advances in subtype classification have successfully stratified the disease using molecular profiling. The contribution of bacterial species to CRC development is increasingly acknowledged, and here, we sought to analyse CRC microbiomes and relate them to tumour consensus molecular subtypes (CMS), in order to better understand the relationship between bacterial species and the molecular mechanisms associated with CRC subtypes. We classified 34 tumours into CRC subtypes using RNA-sequencing derived gene expression and determined relative abundances of bacterial taxonomic groups using 16S rRNA amplicon metabarcoding. 16S rRNA analysis showed enrichment of Fusobacteria and Bacteroidetes, and decreased levels of Firmicutes and Proteobacteria in CMS1. A more detailed analysis of bacterial taxa using non-human RNA-sequencing reads uncovered distinct bacterial communities associated with each molecular subtype. The most highly enriched species associated with CMS1 included Fusobacterium hwasookii and Porphyromonas gingivalis. CMS2 was enriched for Selenomas and Prevotella species, while CMS3 had few significant associations. Targeted quantitative PCR validated these findings and also showed an enrichment of Fusobacterium nucleatum, Parvimonas micra and Peptostreptococcus stomatis in CMS1. In this study, we have successfully associated individual bacterial species to CRC subtypes for the first time.

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Authors
Purcell, R. V., Visnovska, M., Biggs, P. J., Schmeier, S., & Frizelle, F. A.
Affiliations
  • University of Otago (NZ)
  • Massey University (NZ)
Corresponding author
Purcell, Rachel V.
Venue
Scientific Reports
Published
2017-09-14
Volume
7
Issue
1
Pages
11590-11590
ISSN
2045-2322 (ISSN-L)
OpenAlex
W2950036522
Cited by
227
References
60
Citations by year
2026: 16 · 2025: 24 · 2024: 21 · 2023: 34 · 2022: 33 · 2021: 40 · 2020: 20 · 2019: 21 · 2018: 17
FWCI
6.48
Type
journal article
Language
en
Open access
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Hugh Green Foundation
  • Maurice and Phyllis Paykel Trust
  • Colorectal Surgical Society of Australia and New Zealand
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Medicine › Oncology › Colorectal Cancer Treatments and Studies
Keywords
colorectal cancer, gut microbiota, consensus molecular subtypes, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella, bacterial taxa, tumor molecular subtypes, 16S rRNA metabarcoding
MeSH terms
Gastrointestinal Microbiome, Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, RNA, Ribosomal, 16S, Biomarkers, Tumor, Colorectal Neoplasms, Reproducibility of Results, Gene Expression Regulation, Neoplastic, Computational Biology, Gene Expression Profiling, Metagenome, Metagenomics, Transcriptome

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