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Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering
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Brain is an immensely complex system displaying dynamic and heterogeneous metabolic activities. Visualizing cellular metabolism of nucleic acids, proteins, and lipids in brain with chemical specificity has been a long-standing challenge. Recent development in metabolic labeling of small biomolecules allows the study of these metabolisms at the global level. However, these techniques generally require nonphysiological sample preparation for either destructive mass spectrometry imaging or secondary labeling with relatively bulky fluorescent labels. In this study, we have demonstrated bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling. Heterogeneous metabolic incorporations for different molecular species and neurogenesis with newly-incorporated DNA were observed in the dentate gyrus of hippocampus at the single cell level. We further applied this platform to study metabolic responses to traumatic brain injury in hippocampal slice cultures, and observed marked upregulation of protein and lipid metabolism particularly in the hilus region of the hippocampus within days of mechanical injury. Thus, our method paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.
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Cites
A simple method for organotypic cultures of nervous tissue
Stoppini, L., Buchs, P.-A., & Muller, D. (1991) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(91)90128-m · Cited by 3,089
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
Freudiger, C. W., Min, W., Saar, B. G., Lu, S., Holtom, G. R., He, C., Tsai, J. C., Kang, J. X., & Xie, X. S. (2008) · Science · doi:10.1126/science.1165758 · Cited by 2,255
Self-citationCreating new fluorescent probes for cell biology
Zhang, J., Campbell, R. E., Ting, A. Y., & Tsien, R. Y. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm976 · Cited by 1,981
A chemical method for fast and sensitive detection of DNA synthesis in vivo
Salic, A., & Mitchison, T. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0712168105 · Cited by 1,861
Copper-free click chemistry for dynamic in vivo imaging
Baskin, J. M., Prescher, J. A., Laughlin, S. T., Agard, N. J., Chang, P. V., Miller, I. A., Lo, A., Codelli, J. A., & Bertozzi, C. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0707090104 · Cited by 1,833
Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus
Cameron, H. A., & Mckay, R. D. G. (2001) · Journal of Comparative Neurology · doi:10.1002/cne.1040 · Cited by 1,585
Prescher, J. A., & Bertozzi, C. R. (2005) · Nature Chemical Biology · doi:10.1038/nchembio0605-13 · Cited by 1,456
Differentiation of newly born neurons and glia in the dentate gyrus of the adult rat
Cameron, H. A., Woolley, C. S., McEwen, B. S., & Gould, E. (1993) · Neuroscience · doi:10.1016/0306-4522(93)90335-d · Cited by 1,215
Andersen, J. S., Lam, Y. W., Leung, A. K. L., Ong, S.-E., Lyon, C. E., Lamond, A. I., & Mann, M. (2005) · Nature · doi:10.1038/nature03207 · Cited by 1,149
Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine
Cheng, J.-X., & Xie, X. S. (2015) · Science · doi:10.1126/science.aaa8870 · Cited by 874
Schlessinger, A. R., Cowan, W. M., & Gottlieb, D. I. (1975) · Journal of Comparative Neurology · doi:10.1002/cne.901590202 · Cited by 731
Exploring RNA transcription and turnover in vivo by using click chemistry
Jao, C. Y., & Salic, A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0808480105 · Cited by 693
Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy
Min, W., Freudiger, C. W., Lu, S., & Xie, X. S. (2011) · Annual Review of Physical Chemistry · doi:10.1146/annurev.physchem.012809.103512 · Cited by 604
Self-citationFarooqui, A. A., Horrocks, L. A., & Farooqui, T. (2000) · Chemistry and Physics of Lipids · doi:10.1016/s0009-3084(00)00128-6 · Cited by 555
High-speed molecular spectral imaging of tissue with stimulated Raman scattering
Ozeki, Y., Umemura, W., Otsuka, Y., Satoh, S., Hashimoto, H., Sumimura, K., Nishizawa, N., Fukui, K., & Itoh, K. (2012) · Nature Photonics · doi:10.1038/nphoton.2012.263 · Cited by 518
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Wei, L., Hu, F., Shen, Y., Chen, Z., Yu, Y., Lin, C.-C., Wang, M. C., & Min, W. (2014) · Nature Methods · doi:10.1038/nmeth.2878 · Cited by 504
Self-citationChemically sensitive bioimaging with coherent Raman scattering
Camp Jr, C. H., & Cicerone, M. T. (2015) · Nature Photonics · doi:10.1038/nphoton.2015.60 · Cited by 472
Alkyne-Tag Raman Imaging for Visualization of Mobile Small Molecules in Live Cells
Yamakoshi, H., Dodo, K., Palonpon, A., Ando, J., Fujita, K., Kawata, S., & Sodeoka, M. (2012) · Journal of the American Chemical Society · doi:10.1021/ja308529n · Cited by 461
Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy
Yamakoshi, H., Dodo, K., Okada, M., Ando, J., Palonpon, A., Fujita, K., Kawata, S., & Sodeoka, M. (2011) · Journal of the American Chemical Society · doi:10.1021/ja108404p · Cited by 356
Lechene, C., Hillion, F., McMahon, G., Benson, D., Kleinfeld, A. M., Kampf, J. P., Distel, D., Luyten, Y., Bonventre, J., Hentschel, D., et al. (2006) · Journal of Biology · doi:10.1186/jbiol42 · Cited by 346
Chemical reporters for biological discovery
Grammel, M., & Hang, H. C. (2013) · Nature Chemical Biology · doi:10.1038/nchembio.1296 · Cited by 342
Detection of human brain tumor infiltration with quantitative stimulated Raman scattering microscopy
Ji, M., Lewis, S., Camelo-Piragua, S., Ramkissoon, S. H., Snuderl, M., Venneti, S., Fisher-Hubbard, A., Garrard, M., Fu, D., Wang, A. C., et al. (2015) · Science Translational Medicine · doi:10.1126/scitranslmed.aab0195 · Cited by 320
Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals
Berényi, A., Somogyvári, Z., Nagy, A. J., Roux, L., Long, J. D., Fujisawa, S., Stark, E., Leonardo, A., Harris, T. D., & Buzsáki, G. (2014) · Journal of Neurophysiology · doi:10.1152/jn.00785.2013 · Cited by 311
Fluorescence Visualization of Newly Synthesized Proteins in Mammalian Cells
Beatty, K. E., Liu, J. C., Xie, F., Dieterich, D. C., Schuman, E. M., Wang, Q., & Tirrell, D. A. (2006) · Angewandte Chemie International Edition · doi:10.1002/anie.200602114 · Cited by 304
The Epidemiology of Traumatic Brain Injury in Children and Youths: A Review of Research Since 1990
Thurman, D. J. (2016) · Journal of Child Neurology · doi:10.1177/0883073814544363 · Cited by 302
Metabolic labeling and direct imaging of choline phospholipids in vivo
Jao, C. Y., Roth, M., Welti, R., & Salic, A. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0907864106 · Cited by 251
Wei, L., Yu, Y., Shen, Y., Wang, M. C., & Min, W. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1303768110 · Cited by 248
Self-citationMeaney, D. F., Morrison, B., & Dale Bass, C. (2014) · Journal of Biomechanical Engineering · doi:10.1115/1.4026364 · Cited by 247
Self-citationEpidemiology of Concussion and Mild Traumatic Brain Injury
Laker, S. R. (2011) · PM&R · doi:10.1016/j.pmrj.2011.07.017 · Cited by 229
Chemical contrast for imaging living systems: molecular vibrations drive CARS microscopy
Pezacki, J. P., Blake, J. A., Danielson, D. C., Kennedy, D. C., Lyn, R. K., & Singaravelu, R. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.525 · Cited by 226
Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia
Zhang, D.-S., Piazza, V., Perrin, B. J., Rzadzinska, A. K., Poczatek, J. C., Wang, M., Prosser, H. M., Ervasti, J. M., Corey, D. P., & Lechene, C. P. (2012) · Nature · doi:10.1038/nature10745 · Cited by 224
Fu, D., Yu, Y., Folick, A., Currie, E., Farese, R. V., Tsai, T.-H., Xie, X. S., & Wang, M. C. (2014) · Journal of the American Chemical Society · doi:10.1021/ja504199s · Cited by 218
Live‐Cell Stimulated Raman Scattering Imaging of Alkyne‐Tagged Biomolecules
Hong, S., Chen, T., Zhu, Y., Li, A., Huang, Y., & Chen, X. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201400328 · Cited by 203
Krafft, C., Schie, I. W., Meyer, T., Schmitt, M., & Popp, J. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00564g · Cited by 198
Wei, L., Hu, F., Chen, Z., Shen, Y., Zhang, L., & Min, W. (2016) · Accounts of Chemical Research · doi:10.1021/acs.accounts.6b00210 · Cited by 198
Self-citationHilar mossy cells of the dentate gyrus: a historical perspective
Scharfman, H. E., & Myers, C. E. (2013) · Frontiers in Neural Circuits · doi:10.3389/fncir.2012.00106 · Cited by 197
Morrison, B., Cater, H. L., Benham, C. D., & Sundstrom, L. E. (2006) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2005.06.014 · Cited by 196
Self-citationFornix and Hippocampal Atrophy in Traumatic Brain Injury
Tate, D. F., & Bigler, E. D. (2000) · Learning & Memory · doi:10.1101/lm.33000 · Cited by 193
Direct Visualization of De novo Lipogenesis in Single Living Cells
Li, J., & Cheng, J.-X. (2014) · Scientific Reports · doi:10.1038/srep06807 · Cited by 185
Multicolor Live-Cell Chemical Imaging by Isotopically Edited Alkyne Vibrational Palette
Chen, Z., Paley, D. W., Wei, L., Weisman, A. L., Friesner, R. A., Nuckolls, C., & Min, W. (2014) · Journal of the American Chemical Society · doi:10.1021/ja502706q · Cited by 168
Self-citationFast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy
Zhang, D., Wang, P., Slipchenko, M. N., & Cheng, J.-X. (2014) · Accounts of Chemical Research · doi:10.1021/ar400331q · Cited by 167
Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss
Zhang, D., Slipchenko, M. N., & Cheng, J.-X. (2011) · The Journal of Physical Chemistry Letters · doi:10.1021/jz200516n · Cited by 165
Bioorthogonal Chemical Reporters for Analyzing Protein Lipidation and Lipid Trafficking
Hang, H. C., Wilson, J. P., & Charron, G. (2011) · Accounts of Chemical Research · doi:10.1021/ar200063v · Cited by 164
Hu, F., Chen, Z., Zhang, L., Shen, Y., Wei, L., & Min, W. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201502543 · Cited by 164
Self-citationTemporal development of hippocampal cell death is dependent on tissue strain but not strain rate.
Cater, H. L., Sundstrom, L., & Morrison, B. (2006) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2005.09.023 · Cited by 150
Self-citationLee, H. J., Zhang, W., Zhang, D., Yang, Y., Liu, B., Barker, E. L., Buhman, K. K., Slipchenko, L. V., Dai, M., & Cheng, J. (2015) · Scientific Reports · doi:10.1038/srep07930 · Cited by 146
Wei, L., Shen, Y., Xu, F., Hu, F., Harrington, J. K., Targoff, K. L., & Min, W. (2015) · ACS Chemical Biology · doi:10.1021/cb500787b · Cited by 145
Self-citationEnduring suppression of hippocampal long-term potentiation following traumatic brain injury in rat
Miyazaki, S., Katayama, Y., Lyeth, B. G., Jenkins, L. W., DeWitt, D. S., Goldberg, S. J., Newlon, P. G., & Hayes, R. L. (1992) · Brain Research · doi:10.1016/0006-8993(92)91232-4 · Cited by 138
D’Ambrosio, R., Maris, D. O., Grady, M. S., Winn, H. R., & Janigro, D. (1998) · Brain Research · doi:10.1016/s0006-8993(97)01412-1 · Cited by 136
Organotypic cultures as tools for functional screening in the CNS
Sundström, L., Pringle, A. K., Morrison, B., & Bradley, M. (2005) · Drug Discovery Today · doi:10.1016/s1359-6446(05)03502-6 · Cited by 129
Self-citationLifshitz, J., Friberg, H., Neumar, R. W., Raghupathi, R., Welsh, F. A., Janmey, P., Saatman, K. E., Wieloch, T., Grady, M. S., & McIntosh, T. K. (2003) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/01.wcb.0000040581.43808.03 · Cited by 124
Suhalim, J. L., Boik, J. C., Tromberg, B. J., & Potma, E. O. (2012) · Journal of Biophotonics · doi:10.1002/jbio.201200002 · Cited by 113
Lusardi, T. A., Wolf, J. A., Putt, M., Smith, D. H., & Meaney, D. F. (2004) · Journal of Neurotrauma · doi:10.1089/089771504772695959 · Cited by 105
D38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage
Garcia, A. A., Pfisterer, S. G., Riezman, H., Ikonen, E., & Potma, E. O. (2015) · Journal of Biomedical Optics · doi:10.1117/1.jbo.21.6.061003 · Cited by 96
Hu, F., Wei, L., Zheng, C., Shen, Y., & Min, W. (2014) · The Analyst · doi:10.1039/c3an02281a · Cited by 81
Self-citationMasters, B. R. (2010) · Cited by 67
Cater, H. L., Gitterman, D., Davis, S. M., Benham, C. D., Morrison, B., & Sundström, L. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.04379.x · Cited by 56
Self-citationIsolated Primary Blast Inhibits Long-Term Potentiation in Organotypic Hippocampal Slice Cultures
Vogel, E. W., Effgen, G. B., Patel, T. P., Meaney, D. F., Bass, C. R., & Morrison, B. (2015) · Journal of Neurotrauma · doi:10.1089/neu.2015.4045 · Cited by 37
Self-citationChemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics
Yuet, K. P., & Tirrell, D. A. (2013) · Annals of Biomedical Engineering · doi:10.1007/s10439-013-0878-3 · Cited by 24
Lamprecht, M. R., & Morrison, B. (2015) · Journal of Neurotrauma · doi:10.1089/neu.2015.3912 · Cited by 23
Self-citationRaman Microscopy based on Doubly-Resonant Four-Wave Mixing (DR-FWM)
Weeks, T. J., Wachsmann‐Hogiu, S., & Huser, T. R. (2009) · Optics Express · doi:10.1364/oe.17.017044 · Cited by 20
Lamprecht, M. R., Elkin, B. S., Kesavabhotla, K., Crary, J. F., Hammers, J., Huh, J. W., Raghupathi, R., & Morrison, B. (2016) · Journal of Neurotrauma · doi:10.1089/neu.2015.4306 · Cited by 13
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Metadata
- Authors
- Hu, F., Lamprecht, M. R., Wei, L., Morrison, B., & Min, W.
- Venue
- Scientific Reports
- Published
- 2016-12-21
- Volume
- 6
- Issue
- 1
- Pages
- 39660-39660
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2566147354
- Cited by
- 367
- References
- 62
- FWCI
- 17.83
- Type
- journal article
- Language
- en
- Open access
- gold
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