Candida auris : multi-omics signature of an emerging and multidrug-resistant pathogen
Open accessPreprint — published as 10.1128/msystems.00257-19 · Paper page
Abstract
Candida auris is a recently described pathogenic fungus that is causing invasive outbreaks on all continents. The fungus is of high concern given the numbers of multidrug-resistant strains that have been isolated in distinct sites across the globe. The fact that its diagnosis is still problematic suggests that the spreading of the pathogen remains underestimated. Notably, the molecular mechanisms of virulence and antifungal resistance employed by this new species are largely unknown. In the present work, we compared two clinical isolates of C. auris with distinct drug susceptibility profiles and a Candida albicans reference strain using a multi-omics approach. Our results show that, despite the distinct drug-resistance profile, both C. auris strains appear to be very similar, albeit with a few notable differences. However, when compared to C. albicans both C. auris strains have major differences regarding their carbon utilization and downstream lipid and protein content, suggesting a multi-factorial mechanism of drug resistance. The molecular profile displayed by C. auris helps to explain the antifungal resistance and virulence phenotypes of this new emerging pathogen. Importance: Candida auris was firstly described in Japan in 2009 and has now been the cause of significant outbreaks across the globe. The high number of isolates that are resistant to one or more antifungals, as well as the high mortality rates from patients with bloodstream infections, has caught the attention of the medical mycology, infectious disease and public health communities to this pathogenic fungus. In the current work, we performed a broad multi-omics approach on two clinical isolates isolated in New York, the most affected area in the USA and found that the omic profile of C. auris differs significantly from C. albicans . Besides our insights into C. auris carbon utilization and lipid and protein content, we believe that the availability of these data will enhance our ability to combat this rapidly emerging pathogenic yeast.
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Transcriptome Signatures Predict Phenotypic Variations of Candida auris
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Zamith‐Miranda, D., da Silva, R. P., Couvillion, S., Bredeweg, E., Burnet, M., Coelho, C., Camacho, E., Nimrichter, L., Puccia, R., de Almeida, I. C., et al. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.648524 · Cited by 32 · see citing works
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Niclosamide-loaded nanoparticles disrupt Candida biofilms and protect mice from mucosal candidiasis
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Catch the wave: Metabolomic analyses in human pathogenic fungi
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Transcriptional and translational landscape of <i>Candida auris</i> in response to caspofungin
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Self-citationA detailed lipidomic study of human pathogenic fungi Candida auris
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Candida Infections: The Role of Saliva in Oral Health—A Narrative Review
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Melanization of Candida auris Is Associated with Alteration of Extracellular pH
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Self-citationInsights into the Multi-Azole Resistance Profile in Candida haemulonii Species Complex
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Stepping out of the dark: how metabolomics shed light on fungal biology
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Activities of nine antifungal agents against Candida auris biofilms
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Self-citationCellular and Extracellular Vesicle RNA Analysis in the Global Threat Fungus Candida auris
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Self-citationA Histoplasma capsulatum Lipid Metabolic Map Identifies Antifungal Targets
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Self-citationSphingolipid diversity in Candida auris: unraveling interclade and drug resistance fingerprints
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Psychogenetic, genetic and epigenetic mechanisms in Candida auris: Role in drug resistance
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Two Cases of the Emerging Candida auris in a university hospital from Saudi Arabia
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Self-citationThe sphingolipid inhibitor myriocin increases Candida auris susceptibility to amphotericin B
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Highly Reactive Group I Introns Ubiquitous in Pathogenic Fungi
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AFM evaluation of a humanized recombinant antibody affecting C. auris cell wall and stability
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Self-citationA Comprehensive Analysis of the Lipidomic Signatures in Rhizopus delemar
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Deciphering cargo contents in extracellular vesicles of Candida haemulonii var. vulnera
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Mass Spectrometry-based Proteomics of Eukaryotes
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Genomic landscape of the DHA1 family in Candida auris and mapping substrate repertoire of CauMdr1
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Self-citationManaging Candida auris in dialysis units: challenges, strategies, and future directions
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Revisiting threats associated with neglected and emerging fungal pathogens in sub-Saharan Africa
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Self-citationOmics and Multiomics-Based Diagnostics for Invasive Candidiasis: Toward Precision Medicine
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Fungal Sphingolipids: Biosynthesis Pathways, Structural Features and Biological Functions
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2-ethyl-1-hexanol: a promising molecule against priority fungal pathogens
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Unraveling clade-specific differences in Candida auris: evolution, adaptation, and pathogenicity
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Genomic Innovation and Virulence Evolution in the Emerging Human Fungal Pathogen Candida auris
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- Authors
- Zamith-Miranda, D., Heyman, H. M., Cleare, L. G., Couvillion, S., Clair, G., Bredeweg, E., Gacser, A., Nimrichter, L., Nakayasu, E. S., & Nosanchuk, J. D.
- Affiliations
- Pacific Northwest National Laboratory (US)
- Albert Einstein College of Medicine (US)
- Universidade Federal do Rio de Janeiro (BR)
- Corresponding author
- Nosanchuk, Joshua D.
- ORCID
- Venue
- mSystems
- Publisher
- openRxiv
- Published
- 2019-01-23
- Volume
- 4
- Issue
- 4
- ISSN
- 2379-5077 (ISSN-L)
- PMC
- PMC6561322
- OpenAlex
- W2950798794
- Cited by
- 99
- References
- 61
- Citations by year
- 2026: 10 · 2025: 14 · 2024: 13 · 2023: 8 · 2022: 16 · 2021: 15 · 2020: 20 · 2019: 3
- FWCI
- 10.34
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- mSystems (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- SZTE Publicatio Repozitórium (University of Szeged) (acceptedVersion, other-oa)
- Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- U.S. Department of Energy
- Emberi Eroforrások Minisztériuma (Hungary grant 20391-3/2018/FEKUSTRAT, 20391‐3/2018/FEKUSTRAT, 20391-3/2018)
- Nemzeti Kutatási Fejlesztési és Innovációs Hivatal (20391-3/2018/FEKUSTRAT, K 123952)
- National Institutes of Health (20391-3)
- National Research, Development and Innovation Office (K 123952)
- National Institute of Allergy and Infectious Diseases (R21AI124797)
- Pacific Northwest National Laboratory
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida auris, Candida albicans, multidrug resistance, antifungal resistance, multi-omics, clinical isolates, carbon utilization, lipid content, protein content, emerging fungal pathogens, bloodstream infection, comparative genomics
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