PreprintCited by 99Antifungal resistance and susceptibilityFungal and yeast genetics researchYeasts and Rust Fungi Studies

Candida auris : multi-omics signature of an emerging and multidrug-resistant pathogen

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Zamith-Miranda, Daniel, Heyman, Heino M., Cleare, Levi G., Couvillion, Sneha, Clair, Geremy, Bredeweg, Erin, Gacser, Attila, Nimrichter, Leonardo +2 more·2019·mSystems

Preprint — published as 10.1128/msystems.00257-19 · Paper page

Abstract

Candida auris is a recently described pathogenic fungus that is causing invasive outbreaks on all continents. The fungus is of high concern given the numbers of multidrug-resistant strains that have been isolated in distinct sites across the globe. The fact that its diagnosis is still problematic suggests that the spreading of the pathogen remains underestimated. Notably, the molecular mechanisms of virulence and antifungal resistance employed by this new species are largely unknown. In the present work, we compared two clinical isolates of C. auris with distinct drug susceptibility profiles and a Candida albicans reference strain using a multi-omics approach. Our results show that, despite the distinct drug-resistance profile, both C. auris strains appear to be very similar, albeit with a few notable differences. However, when compared to C. albicans both C. auris strains have major differences regarding their carbon utilization and downstream lipid and protein content, suggesting a multi-factorial mechanism of drug resistance. The molecular profile displayed by C. auris helps to explain the antifungal resistance and virulence phenotypes of this new emerging pathogen. Importance: Candida auris was firstly described in Japan in 2009 and has now been the cause of significant outbreaks across the globe. The high number of isolates that are resistant to one or more antifungals, as well as the high mortality rates from patients with bloodstream infections, has caught the attention of the medical mycology, infectious disease and public health communities to this pathogenic fungus. In the current work, we performed a broad multi-omics approach on two clinical isolates isolated in New York, the most affected area in the USA and found that the omic profile of C. auris differs significantly from C. albicans . Besides our insights into C. auris carbon utilization and lipid and protein content, we believe that the availability of these data will enhance our ability to combat this rapidly emerging pathogenic yeast.

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Authors
Zamith-Miranda, D., Heyman, H. M., Cleare, L. G., Couvillion, S., Clair, G., Bredeweg, E., Gacser, A., Nimrichter, L., Nakayasu, E. S., & Nosanchuk, J. D.
Affiliations
  • Pacific Northwest National Laboratory (US)
  • Albert Einstein College of Medicine (US)
  • Universidade Federal do Rio de Janeiro (BR)
Corresponding author
Nosanchuk, Joshua D.
Venue
mSystems
Publisher
openRxiv
Published
2019-01-23
Volume
4
Issue
4
ISSN
2379-5077 (ISSN-L)
OpenAlex
W2950798794
Cited by
99
References
61
Citations by year
2026: 10 · 2025: 14 · 2024: 13 · 2023: 8 · 2022: 16 · 2021: 15 · 2020: 20 · 2019: 3
FWCI
10.34
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • mSystems (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • SZTE Publicatio Repozitórium (University of Szeged) (acceptedVersion, other-oa)
  • Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • U.S. Department of Energy
  • Emberi Eroforrások Minisztériuma (Hungary grant 20391-3/2018/FEKUSTRAT, 20391‐3/2018/FEKUSTRAT, 20391-3/2018)
  • Nemzeti Kutatási Fejlesztési és Innovációs Hivatal (20391-3/2018/FEKUSTRAT, K 123952)
  • National Institutes of Health (20391-3)
  • National Research, Development and Innovation Office (K 123952)
  • National Institute of Allergy and Infectious Diseases (R21AI124797)
  • Pacific Northwest National Laboratory
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
Keywords
Candida auris, Candida albicans, multidrug resistance, antifungal resistance, multi-omics, clinical isolates, carbon utilization, lipid content, protein content, emerging fungal pathogens, bloodstream infection, comparative genomics

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