Biofilm-Forming Capability of Highly Virulent, Multidrug-Resistant Candida auris
Open accessAbstract
The emerging multidrug-resistant yeast pathogen Candida auris has attracted considerable attention as a source of healthcare-associated infections. We report that this highly virulent yeast has the capacity to form antifungal resistant biofilms sensitive to the disinfectant chlorhexidine in vitro.
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Aggregating strains demonstrate higher biofilm-forming capacity.
“Aggregation seems to play an important role in C. auris biofilm formation, since aggregating strains demonstrated higher biofilm-forming capacity, which was associated with distinct transcriptomic profiles compared to the non-aggregative strains (13, 23, 25, 57).”
From Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i> (2024) · cites this paper as [13]
In The Study (page not provided), the two nonaggregative C. auris strains had biofilm biomass 2.4 and 1.5 times less than C. albicans, while the two aggregative strains had biomass 3.0 and 3.1 times less. The measured aggregative strains therefore formed less biofilm, not more, than the nonaggregative strains. (LLM, conf=0.90)
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Experimental — accuracy not yet measuredClinical features of C. auris infections include biofilm persistence on abiotic surfaces and skin, immune evasion, and antifungal drug resistance.
“The clinical features of these infections include biofilm persistence on abiotic surfaces and skin, immune evasion, and antifungal drug resistance (7–12).”
From Dual function of <i>Candida auris</i> mannosyltransferase, MNT5, in biofilm community protection from antifungal therapy and the host (2025) · cites this paper as [11]
The Conclusions (page not provided) state that C. auris adheres to polymeric surfaces, forms biofilms, and resists antifungal agents, supporting the abiotic-surface and drug-resistance portions of the claim. The paper discusses disinfecting skin but does not establish biofilm persistence on skin or describe immune evasion. (LLM, conf=0.88)
Experimental — accuracy not yet measuredAggregative phenotype affects the virulence, antifungal susceptibility, and biofilm formation of C. auris.
“Emerging evidence shows that aggregative phenotype affects the virulence, antifungal susceptibility, and biofilm formation of C. auris (11, 14, 15).”
From Cell aggregation mediated by <i>ACE2</i> deletion in <i>Candida auris</i> modulates fungal colonization and host immune responses in the skin (2024) · cites this paper as [15]
The Study reports different biofilm biomass among aggregative and nonaggregative C. auris strains and suggests enhanced virulence for the nonaggregative phenotype in a larval model. Its antifungal testing says the strains had mostly similar sensitivity profiles, with some strain-specific exceptions; it does not establish a general effect of aggregative phenotype on antifungal susceptibility. Page numbers are unavailable in our copy. (LLM, conf=0.83)
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Cites
Draft genome of a commonly misdiagnosed multidrug resistant pathogen Candida auris
Chatterjee, S., Alampalli, S. V., Nageshan, R. K., Chettiar, S. T., Joshi, S., & Tatu, U. S. (2015) · BMC Genomics · doi:10.1186/s12864-015-1863-z · Cited by 247 · see citing works
Rajendran, R., Sherry, L., Nile, C. J., Sherriff, A., Johnson, E. M., Hanson, M. F., Williams, C., Munro, C. A., Jones, B. J., & Ramage, G. (2016) · Clinical Microbiology and Infection · doi:10.1016/j.cmi.2015.09.018 · Cited by 228 · see citing works
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Research works printed but not linked: 10
- [1]Satoh K, Makimura K, Hasumi Y, Nishiyama Y, Uchida K, Yamaguchi H. Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital. Microbiol Immunol. 2009; 53: 41– 4. 10.1111/j.1348-0421.2008.00083.x 19161556 · DOI · Paper page
- [2]Kathuria S, Singh PK, Sharma C, Prakash A, Masih A, Kumar A, et al. Multidrug-resistant Candida auris misidentified as Candida haemulonii: characterization by matrix-assisted laser desorption ionization-time of flight mass spectrometry and DNA sequencing and its antifungal susceptibility profile variability by Vitek 2, CLSI broth microdilution, and Etest method. J Clin Microbiol. 2015; 53: 1823– 30. 10.1128/JCM.00367-15 25809970 PMC4432077 · DOI
- [5]Calvo B, Melo AS, Perozo-Mena A, Hernandez M, Francisco EC, Hagen F, et al. First report of Candida auris in America: Clinical and microbiological aspects of 18 episodes of candidemia. J Infect. 2016; 73: 369– 74. 10.1016/j.jinf.2016.07.008 27452195 · DOI
- [6]Kumar D, Banerjee T, Pratap CB, Tilak R. Itraconazole-resistant Candida auris with phospholipase, proteinase and hemolysin activity from a case of vulvovaginitis. J Infect Dev Ctries. 2015; 9: 435– 7. 10.3855/jidc.4582 25881537 · DOI
- [7]Oh BJ, Shin JH, Kim MN, Sung H, Lee K, Joo MY, et al. Biofilm formation and genotyping of Candida haemulonii, Candida pseudohaemulonii, and a proposed new species (Candida auris) isolates from Korea. Med Mycol. 2011; 49: 98– 102. 10.3109/13693786.2010.493563 20560864 · DOI
- [9]Borman AM, Szekely A, Johnson EM. Comparative pathogenicity of United Kingdom isolates of the emerging pathogen Candida auris and other key pathogenic Candida species. mSphere. 2016;1:pii:e00189-16. PMC4990711 27547827
- [11]Ramage G, Vande Walle K, Wickes BL, López-Ribot JL. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother. 2001; 45: 2475– 9. 10.1128/AAC.45.9.2475-2479.2001 11502517 PMC90680 · DOI · Paper page
- [12]Sherry L, Rajendran R, Lappin DF, Borghi E, Perdoni F, Falleni M, et al. Biofilms formed by Candida albicans bloodstream isolates display phenotypic and transcriptional heterogeneity that are associated with resistance and pathogenicity. BMC Microbiol. 2014; 14: 182. 10.1186/1471-2180-14-182 24996549 PMC4105547 · DOI
- [14]Pierce CG, Uppuluri P, Tristan AR, Wormley FL Jr, Mowat E, Ramage G, et al. A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc. 2008; 3: 1494– 500. 10.1038/nprot.2008.141 18772877 PMC2741160 · DOI · Paper page
- [15]Rajendran R, Sherry L, Deshpande A, Johnson EM, Hanson MF, Williams C, et al. A prospective surveillance study of candidaemia: epidemiology, risk factors, antifungal treatment and outcome in hospitalized patients. Front Microbiol. 2016; 7: 915. 10.3389/fmicb.2016.00915 27379047 PMC4910670 · DOI
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Forsberg, K., Woodworth, K., Walters, M., Berkow, E. L., Jackson, B., Chiller, T., & Vallabhaneni, S. (2019) · Medical Mycology · doi:10.1093/mmy/myy054 · Cited by 421 · see citing works
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Escandón, P., Chow, N. A., Caceres, D. H., Gade, L., Berkow, E. L., Armstrong, P., Rivera, S., Misas, E., Duarte, C., Moulton-Meissner, H., et al. (2019) · Clinical Infectious Diseases · doi:10.1093/cid/ciy411 · Cited by 222 · see citing works
Kean, R., Delaney, C., Sherry, L., Borman, A., Johnson, E. M., Richardson, M. D., Rautemaa-Richardson, R., Williams, C., & Ramage, G. (2018) · mSphere · doi:10.1128/msphere.00334-18 · Cited by 220 · see citing works
Self-citationProctor, D. M., Dangana, T., Sexton, D. J., Fukuda, C., Yelin, R. D., Stanley, M., Bell, P. B., Baskaran, S., Deming, C., Chen, Q., et al. (2021) · Nature Medicine · doi:10.1038/s41591-021-01383-w · Cited by 198 · see citing works
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“05), consistent with prior studies demonstrating lower biofilm burdens for this species (8, 9).”
ResultAbdolrasouli, A., Armstrong‐James, D., Ryan, L., & Schelenz, S. (2017) · Mycoses · doi:10.1111/myc.12699 · Cited by 150 · see citing works
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Sabino, R., Veríssimo, C., Pereira, Á. A., & Antunes, F. (2020) · Microorganisms · doi:10.3390/microorganisms8020181 · Cited by 133 · see citing works
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Dominguez, E. G., Zarnowski, R., Choy, H. L., Zhao, M., Sanchez, H., Nett, J. E., & Andes, D. R. (2019) · mSphere · doi:10.1128/mspheredirect.00680-18 · Cited by 132 · see citing works
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Horton, M. V., & Nett, J. E. (2020) · Current Clinical Microbiology Reports · doi:10.1007/s40588-020-00143-7 · Cited by 132 · see citing works
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Muñoz, J. F., Welsh, R. M., Shea, T., Batra, D., Gade, L., Howard, D., Rowe, L. A., Meis, J. F., Litvintseva, A. P., & Cuomo, C. A. (2021) · Genetics · doi:10.1093/genetics/iyab029 · Cited by 125 · see citing works
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Rossato, L., & Colombo, A. L. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.03081 · Cited by 121 · see citing works
Lara, H. H., Ixtepan-Turrent, L., Jose Yacaman, M., & Lopez-Ribot, J. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b20708 · Cited by 116 · see citing works
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Wang, Y., Zou, Y., Chen, X., Li, H., Yin, Z., Zhang, B., Xu, Y., Zhang, Y., Zhang, R., Huang, X., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-31201-x · Cited by 107 · see citing works
Kenters, N., Kiernan, M., Chowdhary, A., Denning, D. W., Pemán, J., Saris, K., Schelenz, S., Tartari, E., Widmer, A., Meis, J. F., et al. (2019) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2019.08.013 · Cited by 102 · see citing works
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Nett, J. E. (2019) · PLOS Pathogens · doi:10.1371/journal.ppat.1007638 · Cited by 69 · see citing works
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- Authors
- Sherry, L., Ramage, G., Kean, R., Borman, A., Johnson, E. M., Richardson, M. D., & Rautemaa-Richardson, R.
- ORCID
- Venue
- Emerging Infectious Diseases
- Published
- 2017-02
- Volume
- 23
- Issue
- 2
- Pages
- 328-331
- ISSN
- 1080-6040 (ISSN-L)
- 1080-6059
- PMC
- PMC5324806
- OpenAlex
- W2572527541
- Cited by
- 420
- References
- 2
- Citations by year
- 2026: 38 · 2025: 45 · 2024: 54 · 2023: 55 · 2022: 38 · 2021: 41 · 2020: 55 · 2019: 39 · 2018: 23 · 2017: 29
- FWCI
- 30.73
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by
- Locations
- Emerging infectious diseases (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Research Explorer (The University of Manchester) (submittedVersion, other-oa)
- Funders
- University of Glasgow
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Infectious Diseases › Infection Control in Healthcare
- Keywords
- Candida auris, biofilm formation, multidrug resistance, antifungal resistance, chlorhexidine, disinfectant susceptibility, virulence, nosocomial infections, pathogenic yeasts
- MeSH terms
- Animals, Antifungal Agents, Candida, Candidiasis, Larva, Microbial Sensitivity Tests, Moths, Virulence, Biofilms, Drug Resistance, Multiple, Fungal
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