CoChat
Sign Up
Cited by 544

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity

Ronco, Lucienne V., Karpova, Alla Y., Vidal, Marc, Howley, Peter M.·1998·Genes & Development
DOI 10.1101/gad.12.13.2061
Open this paper in CoChat — free account

Abstract

Interferon regulatory factor-3 (IRF-3) was found to specifically interact with HPV16 E6 in a yeast two-hybrid screen. IRF-3 is activated by the presence of double-stranded RNA or by virus infection to form a stable complex with other transcriptional regulators that bind to the regulatory elements of the IFNβ promoter. We show that IRF-3 is a potent transcriptional activator and demonstrate that HPV16 E6 can inhibit its transactivation function. The expression of HPV16 E6 in primary human keratinocytes inhibits the induction of IFNβ mRNA following Sendai virus infection. The binding of HPV16 E6 to IRF-3 does not result in its ubiquitination or degradation. We propose that the interaction of E6 with IRF-3 and the inhibition of IRF-3’s transcriptional activity may provide the virus a means to circumvent the normal antiviral response of an HPV16-infected cell.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/gad.12.13.2061

Cites

6 of 6 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 6 references

Research works printed but not linked: 63

  1. Androphy, 1987, Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines., EMBO J., 6, 989, 10.1002/j.1460-2075.1987.tb04849.x · DOI
  2. 10.1073/pnas.92.25.11657 · DOI
  3. Band, 1993, Enhanced degradation of p53 protein in HPV-6 and BPV-1 E6 immortalized human mammary epithelial cells., EMBO J., 12, 1847, 10.1002/j.1460-2075.1993.tb05833.x · DOI
  4. 10.1038/383344a0 · DOI · Paper page
  5. 10.1093/jnci/87.11.796 · DOI
  6. 10.1073/pnas.91.11.5046 · DOI · Paper page
  7. Brutkiewiewicz, 1995, Major histocompatibility complex class I antigens and the control of viral infections by natural killer cells., J. Virol., 69, 3967, 10.1128/jvi.69.7.3967-3971.1995 · DOI
  8. 10.1126/science.7624774 · DOI · Paper page
  9. 10.1016/0092-8674(91)90529-8 · DOI
  10. De Maeyer E. De Maeyer-Guignard J. (1988) Interferons and other regulatory cytokines. (John Wiley and Sons, New York, NY).
  11. 10.1101/gad.7.3.479 · DOI
  12. Desaintes, 1992, Transcriptional activation of several heterologous promoters by the E6 protein of human papillomavirus type 16., J. Virol., 66, 325, 10.1128/jvi.66.1.325-333.1992 · DOI
  13. 10.1006/viro.1996.8363 · DOI
  14. Dowhanick, 1995, Suppression of cellular proliferation by the papillomavirus E2 protein., J. Virol., 69, 7791, 10.1128/jvi.69.12.7791-7799.1995 · DOI
  15. 10.1093/nar/20.10.2533 · DOI
  16. Elston, 1998, The identification of a conserved binding motif within human papillomavirus type 16 E6 binding peptides, E6AP, E6BP., J. Gen. Virol., 79, 371, 10.1099/0022-1317-79-2-371 · DOI
  17. 10.1006/viro.1994.1684 · DOI
  18. 10.1016/S0952-7915(96)80035-5 · DOI
  19. 10.1038/337270a0 · DOI
  20. Halbert, 1991, The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells., J. Virol., 65, 473, 10.1128/jvi.65.1.473-478.1991 · DOI · Paper page
  21. 10.1016/0092-8674(90)90163-9 · DOI
  22. 10.1126/science.8438157 · DOI
  23. Harlow E. Lane D. (1988) Antibodies: A laboratory manual. (Cold Spring Harbor Press, Cold Spring Harbor Laboratory, NY).
  24. 10.1016/0092-8674(92)90586-2 · DOI
  25. Howley P.M. (1996) Papillomavirinae: The viruses and their replication. in Fundamental Virology, eds Bernard D.M.K. Fields N. Howley P.M. (Lippincott-Raven, Philadelphia, PA), pp 2045–20076.
  26. Hudson, 1990, Immortalization and altered differentiation of human keratinocytes in vitro by the E6 and E7 open reading frames of human papillomavirus type 18., J. Virol., 64, 519, 10.1128/jvi.64.2.519-526.1990 · DOI · Paper page
  27. 1993, Cloning and expression of the cDNA for E6-AP: A protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53., Mol. Cell. Biol., 13, 775
  28. 1993, Localization of the E6-AP regions that direct HPV E6 binding, association with p53, and ubiquitination of associated proteins., Mol. Cell. Biol., 13, 4918
  29. 10.1006/scbi.1996.0042 · DOI
  30. 10.1016/S1097-2765(00)80013-1 · DOI
  31. 10.1006/viro.1997.8536 · DOI
  32. 10.1038/380079a0 · DOI · Paper page
  33. Lamberti, 1990, Transcriptional activation by the papillomavirus E6 zinc finger oncoprotein., EMBO J., 9, 1907, 10.1002/j.1460-2075.1990.tb08317.x · DOI
  34. Lechner, 1992, Human papillomavirus E6 proteins bind p53 in vivo and abrogate p53-mediated repression of transcription., EMBO J., 11, 3045, 10.1002/j.1460-2075.1992.tb05375.x · DOI
  35. 10.1101/gad.2.4.383 · DOI
  36. Lowe, 1993, p53-dependent apoptosis modulates the cytotoxicity of anticancer agents., Cell, 74, 847, 10.1016/0092-8674(93)90719-7 · DOI
  37. Mietz, 1992, The transcriptional transactivation function of wild-type p53 is inhibited by SV40 large T-antigen and by HPV-16 oncoprotein., EMBO J., 11, 5013, 10.1002/j.1460-2075.1992.tb05608.x · DOI
  38. Miller, 1989, Improved retroviral vectors for gene transfer and expression., BioTechniques, 7, 980
  39. 10.1126/science.8009221 · DOI
  40. Münger, 1989, The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes., J. Virol., 63, 4417, 10.1128/jvi.63.10.4417-4421.1989 · DOI · Paper page
  41. 10.1101/gad.8.11.1285 · DOI
  42. Reis, 1992, Critical role of a common transcription factor, IRF-1, in the regulation if IFN-α and IFN-β inducible genes., EMBO J., 11, 185, 10.1002/j.1460-2075.1992.tb05041.x · DOI
  43. 10.1016/S0092-8674(94)90462-6 · DOI · Paper page
  44. 10.1093/nar/17.18.7539 · DOI
  45. Sakai, 1996, Targeted mutagenesis of the human papillomavirus type 16 E2 transactivation domain reveals separable transcriptional activation and DNA replication functions., J. Virol., 70, 1602, 10.1128/jvi.70.3.1602-1611.1996 · DOI
  46. 10.1074/jbc.273.5.2714 · DOI
  47. 10.1073/pnas.88.13.5523 · DOI
  48. Sedman, 1991, The full-length E6 protein of human papillomavirus type 16 has transforming and trans-activating activities and cooperates with E7 to immortalize keratinocytes in culture., J. Virol., 65, 4860, 10.1128/jvi.65.9.4860-4866.1991 · DOI
  49. Sherman, 1996, Serum-and calcium-induced differentiation of human keratinocytes is inhibited by the E6 oncoprotein of human papillomavirus type 16., J. Virol., 70, 3269, 10.1128/jvi.70.5.3269-3279.1996 · DOI
  50. Shino, 1997, Human papillomavirus type 16 E6 protein transcriptionally modulates fibronectin gene expression by induction of protein complexes binding to the cylclic AMP response element., J. Virol., 71, 4310, 10.1128/jvi.71.6.4310-4318.1997 · DOI
  51. 10.1006/viro.1994.1452 · DOI
  52. 10.1038/376596a0 · DOI
  53. 10.1038/382816a0 · DOI
  54. 10.1016/0092-8674(95)90136-1 · DOI · Paper page
  55. 10.1073/pnas.94.9.4412 · DOI
  56. 10.1038/377362a0 · DOI
  57. Vilcek J. Sen G.S. (1996) Interferons and other cytokines. in Fields virology, eds Fields B.N. Howley P. M. Knipe D.M. (Raven Press, New York, NY), 3rd. ed. pp 375–400.
  58. Wathelet, 1992, Regulation of gene expression by cytokines and virus in human cells lacking the type-1 interferon locus., Eur. J. Biochem., 206, 901, 10.1111/j.1432-1033.1992.tb16999.x · DOI
  59. Weaver, 1998, Interferon regulatory factor 3 and CREB-binding protein/p300 are subunits of double-stranded RNA-activated transcription factor DRAF1., Mol. Cell. Biol., 18, 1359, 10.1128/MCB.18.3.1359 · DOI
  60. 10.1126/science.2157286 · DOI · Paper page
  61. Yasugi, 1997, Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins., J. Virol., 71, 891, 10.1128/jvi.71.2.891-899.1997 · DOI
  62. Zhang, 1997, IRF-7, a new interferon regulatory factor associated with epstein-barr virus latency., Mol. Cell. Biol., 17, 5748, 10.1128/MCB.17.10.5748 · DOI
  63. zur Hausen, 1996, Papillomavirus infections—a major cause of human cancers., Biochim. Biophys. Acta, 1288, F55

Cited by

See all 544

Metadata

Authors
Ronco, L. V., Karpova, A. Y., Vidal, M., & Howley, P. M.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
1998-07-01
Volume
12
Issue
13
Pages
2061-2072
ISSN
  • 0890-9369
  • 1549-5477
DOI
10.1101/gad.12.13.2061
Cited by
544
References
6
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity. https://app.cochat.ai/papers/10.1101/gad.12.13.2061
The paper
Ronco, L. V., Karpova, A. Y., Vidal, M., & Howley, P. M. (1998). Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity. Genes & Development. https://doi.org/10.1101/gad.12.13.2061