Sleep-dependent activity of T cells and regulatory T cells
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Summary: A number of immunological functions are dependent on circadian rhythms and regular sleep. This has impact on the type and magnitude of immune responses following antigenic challenge, for example in vaccination. Little is known about the underlying mechanisms. One possibility may be the circadian and sleep-dependent modulation of CD4+CD25- T cell responses by CD4+CD25+ natural regulatory T cells (nTreg). In a variety of studies, nTreg have been shown to regulate T cell responses negatively. Thus, we investigated the influence of sleep and circadian rhythm on the number and function of nTreg as well as on the function of CD4+CD25- T cells. Seven healthy young men were examined under defined conditions on two occasions, i.e. during sleep and sleep deprivation. Venous blood was drawn periodically; numbers of nTreg, suppressive activity of nTreg, interleukin-2 production and proliferation of CD4+CD25- T cells were explored in vitro. nTreg counts revealed a significant circadian rhythm with highest levels during the night (mean 95 nTreg/µl) and lowest levels during the day (mean 55 nTreg/µl). During normal sleep, the suppressive activity of nTreg was highest at 02.00 h and somewhat lower at 15.00 h. Surprisingly, almost no suppressive activity was present at 07.00 h. Deprivation of sleep abrogated this rhythm. CD4+CD25– T cell proliferation was dampened significantly by sleep deprivation. This is the first study in human cells to show that nTreg number and function follow a rhythm across the 24-h period. Furthermore, sleep deprivation severely disturbs the functional rhythm of nTreg and CD4+CD25– T cells.
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Several reports indicated an enhancing influence of sleep on T-cell production of IL-2 which acts to further promote T-cell growth, proliferation, and differentiation, although other studies failed to reveal such effects when examining IL-2 production in specific T-cell subpopulations.
“Furthermore, several reports indicated an enhancing influence of sleep on T-cell production of IL-2 which acts to further promote T-cell growth, proliferation, and differentiation (120, 595), although other studies failed to reveal such effects when examining IL-2 production in specific T-cell subpopulations (109,308).”
From About Sleep's Role in Memory (2013) · cites this paper as [109]
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Experimental — accuracy not yet measuredSleep increased proliferation of Th cells as well as the activity of natural regulatory T (nTreg) cells.
“Sleep increased proliferation of Th cells as well as the activity of natural regulatory T (nTreg) cells (109).”
From About Sleep's Role in Memory (2013) · cites this paper as [109]
Sleep might support the migration of lymphocytes, thus supporting the formation of immunological memory.
“sleep might rather support the migration of lymphocytes, thus supporting the formation of immunological memory.”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [63]
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- Authors
- Bollinger, T., Bollinger, A., Skrum, L., Dimitrov, S., Lange, T., & Solbach, W.
- Affiliations
- University of Lübeck (DE)
- Research Center Borstel - Leibniz Lung Center (DE)
- Institute of Medical Microbiology and Hygiene (DE)
- Corresponding author
- Bollinger, T
- Venue
- Clinical and Experimental Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-11-27
- Volume
- 155
- Issue
- 2
- Pages
- 231-238
- ISSN
- 0009-9104 (ISSN-L)
- 1365-2249
- PMC
- PMC2675254
- OpenAlex
- W2111292033
- Cited by
- 167
- References
- 53
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 17 · 2023: 9 · 2022: 15 · 2021: 6 · 2020: 7 · 2019: 8 · 2018: 11 · 2017: 15 · 2016: 6 · 2015: 13 · 2014: 8 · 2013: 6 · 2012: 7
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical & Experimental Immunology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Keywords
- circadian rhythm, sleep, sleep deprivation, regulatory T cells, natural regulatory T cells, CD4+CD25+ T cells, interleukin-2, T cell suppression, immune regulation, vaccination
- MeSH terms
- Adult, Cells, Cultured, Circadian Rhythm, Humans, Immune Tolerance, Interleukin-2, Male, Sleep, Sleep Deprivation, CD4-Positive T-Lymphocytes, T-Lymphocyte Subsets, Polysomnography, CD4 Lymphocyte Count, Cell Proliferation, T-Lymphocytes, Regulatory, Forkhead Transcription Factors, Interleukin-2 Receptor alpha Subunit, Young Adult
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