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RNA-Guided Human Genome Engineering via Cas9
Open accessAbstract
Genome Editing: Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by van der Oost ). Cong et al. (p. 819 , published online 3 January) and Mali et al. (p. 823 , published online 3 January) adapted this defense system to function as a genome editing tool in eukaryotic cells.
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“Upon target recognition, the DNA strands are opened in an R-loop and individually cleaved, which results in a double-strand break (DSB)22,23”
From One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids (2023) · cites this paper as [23]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The technique also allows DNA sequence changes in pluripotent embryonic stem cells (4) that can then be cultured to produce specific tissues, such as cardiomyocytes or neurons (5).”
From A prudent path forward for genomic engineering and germline gene modification (2015) · cites this paper as [4]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In January 2013, almost simultaneously, five research articles authored by different research teams appeared, all reporting that they had achieved the goal.”
From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [34]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Mali et al. created a novel version of the genome-editing tool applicable to mammalian cells, termed the CRISPR/Cas9 system, which is based on modifications of the Streptococcus pyogenes type II CRISPR system in crRNA fused to trans-encoded tracrRNA 13 .”
From Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus (2013) · cites this paper as [13]
The cited source says what the claim attributes to it.
In the citing paper
“The pigs were engineered to carry 69 genomic edits, using the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9)-mediated nonhomologous end joining and homology-directed repair30,31, and recombinase-mediated cassette exchange32 (Fig. 2a, Extended Data Fig. 1a and Supplementary Table 2).”
From Design and testing of a humanized porcine donor for xenotransplantation (2023) · cites this paper as [31]
In the citing paper
“When a double-stranded break is introduced into genomic DNA, cells can repair the break by using a DNA molecule with high sequence similarity as a template. If an artificial template is provided while the DNA break is introduced, this pathway provides a mean to incorporate desired DNA sequences into the genome34,35”
From CRISPR-based functional genomics for neurological disease (2020) · cites this paper as [35]
In the citing paper
“The discovery, made less than 10 years ago, that a simple two-component system of a Cas protein (Streptococcus pyogenes Cas9) bound to a single guide RNA (sgRNA) can function as a programmable nuclease33 that can be deployed to cleave specific sequences in mammalian genomes34,35 has been transformative for biomedical research and the development of gene therapies.”
From CRISPR-based functional genomics for neurological disease (2020) · cites this paper as [35]
In the citing paper
“Recently, the CRISPR/Cas system has been successfully applied to human cells19,20,21,22 and several model organisms for genome editing.23,24,25,26”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [19]
In the citing paper
“By bioinformatic screening and efficiency/off-target prediction (14, 15), we identified four gRNA targets (protospacers; LTRs A-D) that avoid conserved transcription factor binding sites, minimizing the likelihood of altering host gene expression”
From RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection (2014) · cites this paper as [15]
In the citing paper
“Compared with well-known, site-specific genome editing technologies, such as ZFNs and TALENs, the new CRISPR-Cas approach is more convenient and efficient [10], [12].”
From High-efficiency targeted editing of large viral genomes by RNA-guided nucleases (2014) · cites this paper as [10]
In the citing paper
“Clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 is a naturally occurring bacterial immune system that uses a novel nuclease system to protect bacteria from phage infection (6-9), and it has recently been harnessed for a variety of genome-engineering applications (10)(11)(12)(13)(14)(15)(16)(17).”
From RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection (2014) · cites this paper as [13]
In the citing paper
“Moreover, no significant cytotoxicity was observed [87,88].”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [88]
In the citing paper
“although Cas9 protein naturally induces double stranded DNA breaks by cutting each strand with two separate motifs, a mutation within one of the enzymatic motifs of Cas9 converted it into a valuable nickase with the capability to induce homologous recombination”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [88]
In the citing paper
“and the mutant is much safer to use with less off-target effects [88].”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [88]
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Citations
Cites
BEDTools: a flexible suite of utilities for comparing genomic features
Quinlan, A. R., & Hall, I. M. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq033 · Cited by 27,437
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
Langmead, B., Trapnell, C., Pop, M., & Salzberg, S. L. (2009) · Genome Biology · doi:10.1186/gb-2009-10-3-r25 · Cited by 21,849
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
The Human Genome Browser at UCSC
Kent, W. J., Sugnet, C. W., Furey, T. S., Roskin, K. M., Pringle, T. H., Zahler, A. M., & Haussler, A. D. (2002) · Genome Research · doi:10.1101/gr.229102 · Cited by 10,400
Lorenz, R., Bernhart, S. H., Höner zu Siederdissen, C., Tafer, H., Flamm, C., Stadler, P. F., & Hofacker, I. L. (2011) · Algorithms for Molecular Biology · doi:10.1186/1748-7188-6-26 · Cited by 5,098
A System for Stable Expression of Short Interfering RNAs in Mammalian Cells
Brummelkamp, T. R., Bernards, R., & Agami, R. (2002) · Science · doi:10.1126/science.1068999 · Cited by 4,295
Mathews, D. H., Sabina, J., Zuker, M., & Turner, D. H. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.2700 · Cited by 3,795
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
The accessible chromatin landscape of the human genome
Thurman, R. E., Rynes, E., Humbert, R., Vierstra, J., Maurano, M. T., Haugen, E., Sheffield, N. C., Stergachis, A. B., Wang, H., Vernot, B., et al. (2012) · Nature · doi:10.1038/nature11232 · Cited by 2,679
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Niopek, D., Wehler, P., Roensch, J., Eils, R., & Ventura, B. D. (2016) · Nature Communications · doi:10.1038/ncomms10624 · Cited by 255
YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression
Lyons, S. M., Achorn, C. A., Kedersha, N., Anderson, P., & Ivanov, P. V. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw418 · Cited by 255
Autoimmune diseases — connecting risk alleles with molecular traits of the immune system
Gutiérrez‐Arcelus, M., Rich, S. S., & Raychaudhuri, S. (2015) · Nature Reviews Genetics · doi:10.1038/nrg.2015.33 · Cited by 256
Alagoz, Y., Gurkok, T., Zhang, B., & Ünver, T. (2016) · Scientific Reports · doi:10.1038/srep30910 · Cited by 255
In Vivo Delivery of CRISPR/Cas9 for Therapeutic Gene Editing: Progress and Challenges
Mout, R., Ray, M., Lee, Y.-W., Scaletti, F., & Rotello, V. M. (2017) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.7b00057 · Cited by 256
Rewritable multi-event analog recording in bacterial and mammalian cells
Tang, W., & Liu, D. R. (2018) · Science · doi:10.1126/science.aap8992 · Cited by 255
Streptococcus thermophilus CRISPR-cas9 systems enable specific editing of the human genome
Müller, M., Lee, C. M., Gasiunas, G., Davis, T. H., Cradick, T. J., Siksnys, V., Bao, G., Cathomen, T., & Mussolino, C. (2016) · Molecular Therapy · doi:10.1038/mt.2015.218 · Cited by 254
The transience of transient overexpression
Gibson, T. J., Seiler, M., & Veitia, R. A. (2013) · Nature Methods · doi:10.1038/nmeth.2534 · Cited by 254
Efficient Gene Knockout in Goats Using CRISPR/Cas9 System
Ni, W., Qiao, J., Hu, S., Zhao, X., Regouski, M., Yang, M., Polejaeva, I. A., & Chen, C. (2014) · PLoS ONE · doi:10.1371/journal.pone.0106718 · Cited by 254
van Sluis, M., & McStay, B. (2015) · Genes & Development · doi:10.1101/gad.260703.115 · Cited by 254
Genome Editing with Engineered Nucleases in Plants
Osakabe, Y., & Osakabe, K. (2014) · Plant and Cell Physiology · doi:10.1093/pcp/pcu170 · Cited by 253
Potential pitfalls of CRISPR/Cas9‐mediated genome editing
Peng, R., Lin, G., & Li, J. (2016) · The FEBS Journal · doi:10.1111/febs.13586 · Cited by 213
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Hansen‐Bruhn, M., de Ávila, B. E., Beltrán‐Gastélum, M., Zhao, J. T., Ramírez‐Herrera, D. E., Angsantikul, P., Gothelf, K. V., Zhang, L., & Wang, J. (2018) · Angewandte Chemie International Edition · doi:10.1002/anie.201713082 · Cited by 253
Review of CRISPR/Cas9 sgRNA Design Tools
Cui, Y., Xu, J., Cheng, M., Liao, X., & Peng, S. (2018) · Interdisciplinary Sciences Computational Life Sciences · doi:10.1007/s12539-018-0298-z · Cited by 253
Ormond, K. E., Mortlock, D. P., Scholes, D. T., Bombard, Y., Brody, L. C., Faucett, W. A., Garrison, N. A., Hercher, L., Isasi, R., Middleton, A., et al. (2017) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2017.06.012 · Cited by 252
Lv, L., Ren, Y., Chen, J., Wu, Q., & Chen, G. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.03.013 · Cited by 252
Liang, X., Potter, J., Kumar, S., Ravinder, N., & Chesnut, J. D. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.11.011 · Cited by 252
Chehelgerdi, M., Chehelgerdi, M., Khorramian-Ghahfarokhi, M., Shafieizadeh, M., Mahmoudi, E., Eskandari, F., Rashidi, M., Arshi, A., & Mokhtari-Farsani, A. (2024) · Molecular Cancer · doi:10.1186/s12943-023-01925-5 · Cited by 251
Gao, X., Chen, J., Dai, X., Zhang, D., & Zhao, Y. (2016) · PLANT PHYSIOLOGY · doi:10.1104/pp.16.00663 · Cited by 251
Hematopoietic Stem Cell Gene Therapy: Progress and Lessons Learned
Morgan, R. A., Gray, D., Lomova, A., & Kohn, D. B. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.10.010 · Cited by 252
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants
Kumar, M., Prusty, M. R., Pandey, M. K., Singh, P. K., Bohra, A., Guo, B. Z., & Varshney, R. K. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1157678 · Cited by 251
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Wu, J., Okamura, D., Li, M., Suzuki, K., Luo, C., Ma, L., He, Y., Li, Z., Benner, C. W., Tamura, I., et al. (2015) · Nature · doi:10.1038/nature14413 · Cited by 250
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells
Lee, C. M., Cradick, T. J., & Bao, G. (2016) · Molecular Therapy · doi:10.1038/mt.2016.8 · Cited by 250
Refining strategies to translate genome editing to the clinic
Cornu, T. I., Mussolino, C., & Cathomen, T. (2017) · Nature Medicine · doi:10.1038/nm.4313 · Cited by 252
Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies
Marino, N. D., Pinilla‐Redondo, R., Csörgő, B., & Bondy‐Denomy, J. (2020) · Nature Methods · doi:10.1038/s41592-020-0771-6 · Cited by 250
Sound Strategies for Hearing Restoration
Géléoc, G. S. G., & Holt, J. R. (2014) · Science · doi:10.1126/science.1241062 · Cited by 250
A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis
Zhang, Z., Mao, Y., Ha, S., Liu, W., Botella, J. R., & Zhu, J. (2015) · Plant Cell Reports · doi:10.1007/s00299-015-1900-z · Cited by 249
O’Geen, H., Ren, C., Nicolet, C. M., Perez, A. A., Halmai, J. A. N. M., Le, V. M., Mackay, J. P., Farnham, P., & Segal, D. J. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx578 · Cited by 249
CRISPR-GPT for agentic automation of gene-editing experiments
Qu, Y., Huang, K., Yin, M., Zhan, K., Liu, D., Yin, D., Cousins, H. C., Johnson, W. A., Wang, X., Shah, M. M., et al. (2025) · Nature Biomedical Engineering · doi:10.1038/s41551-025-01463-z · Cited by 266
Targeting non-coding RNAs with the CRISPR/Cas9 system in human cell lines
Ho, T.-T., Zhou, N., Huang, J., Koirala, P., Xu, M., Fung, R., Wu, F., & Mo, Y. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1198 · Cited by 248
Single-cut genome editing restores dystrophin expression in a new mouse model of muscular dystrophy
Amoasii, L., Long, C., Li, H., Mireault, A. A., Shelton, J. M., Sanchez-Ortiz, E., McAnally, J. R., Bhattacharyya, S., Schmidt, F., Grimm, D., et al. (2017) · Science Translational Medicine · doi:10.1126/scitranslmed.aan8081 · Cited by 247
Wu, Y., Zhou, H., Fan, X., Zhang, Y., Zhang, M., Wang, Y., Xie, Z., Bai, M., Yin, Q., Liang, D., et al. (2015) · Cell Research · doi:10.1038/cr.2014.160 · Cited by 247
Huang, J., Liu, X., Li, D., Shao, Z., Cao, H., Zhang, Y., Trompouki, E., Bowman, T. V., Zon, L. I., Yuan, G., et al. (2016) · Developmental Cell · doi:10.1016/j.devcel.2015.12.014 · Cited by 247
Major bacterial lineages are essentially devoid of CRISPR-Cas viral defence systems
Burstein, D. E., Sun, C. L., Brown, C. T., Sharon, I., Anantharaman, K., Probst, A. J., Thomas, B. C., & Banfield, J. F. (2016) · Nature Communications · doi:10.1038/ncomms10613 · Cited by 247
CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing
Dehairs, J., Talebi, A., Chérifi, Y., & Swinnen, J. V. (2016) · Scientific Reports · doi:10.1038/srep28973 · Cited by 247
Targeted mutagenesis in chicken using CRISPR/Cas9 system
Oishi, I., Yoshii, K., Miyahara, D., KAGAMI, H., & Tagami, T. (2016) · Scientific Reports · doi:10.1038/srep23980 · Cited by 246
Phillips-Krawczak, C. A., Singla, A., Starokadomskyy, P., Deng, Z., Osborne, D. G., Li, H., Dick, C. J., Gomez, T. S., Koenecke, M., Zhang, J.-S., et al. (2015) · Molecular Biology of the Cell · doi:10.1091/mbc.e14-06-1073 · Cited by 246
CRISPR-mediated Genome Editing Restores Dystrophin Expression and Function in mdx Mice
Xu, L., Park, K. H., Zhao, L., Xu, J., El Refaey, M., Gao, Y., Zhu, H., Ma, J., & Han, R. (2016) · Molecular Therapy · doi:10.1038/mt.2015.192 · Cited by 244
Artificial Virus Delivers CRISPR-Cas9 System for Genome Editing of Cells in Mice
Li, L., Song, L., Liu, X., Yang, X., Li, X., He, T., Wang, N., Yang, S., Yu, C., Yin, T., et al. (2017) · ACS Nano · doi:10.1021/acsnano.6b04261 · Cited by 244
Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects
Gao, Y., Wu, H., Wang, Y., Liu, X., Chen, L., Li, Q., Cui, C., Liu, X., Zhang, J., & Zhang, Y. (2017) · Genome biology · doi:10.1186/s13059-016-1144-4 · Cited by 246
The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control
Bernard, G. C., Egnin, M., & Bonsi, C. K. (2017) · InTech eBooks · doi:10.5772/intechopen.68958 · Cited by 245
CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point‐of‐Care Diagnosis
Dai, Y., Wu, Y., Liu, G., & Gooding, J. J. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202005398 · Cited by 244
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
Stella, S., Alcón, P., & Montoya, G. (2017) · Nature · doi:10.1038/nature22398 · Cited by 244
Zhang, D., Wang, G., Yu, X., Wei, T., Farbiak, L., Johnson, L. T., Taylor, A. M., Xu, J., Hong, Y., Zhu, H., et al. (2022) · Nature Nanotechnology · doi:10.1038/s41565-022-01122-3 · Cited by 243
Shelake, R. M., Kadam, U. S., Kumar, R., Pramanik, D., Singh, A. K., & Kim, J. (2022) · Plant Communications · doi:10.1016/j.xplc.2022.100417 · Cited by 243
Dissecting super-enhancer hierarchy based on chromatin interactions
Huang, J., Li, K., Cai, W., Liu, X., Zhang, Y., Orkin, S. H., Xu, J., & Yuan, G.-C. (2018) · Nature Communications · doi:10.1038/s41467-018-03279-9 · Cited by 241
The New State of the Art: Cas9 for Gene Activation and Repression
Russa, M. F. L., & Qi, L. S. (2015) · Molecular and Cellular Biology · doi:10.1128/mcb.00512-15 · Cited by 241
Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy
Puente, C., Hendrickson, R. C., & Jiang, X. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.689646 · Cited by 241
Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia
Cuellar, T. L., Herzner, A., Zhang, X., Goyal, Y., Watanabe, C. K., Friedman, B. A., Janakiraman, V., Durinck, S., Stinson, J., Arnott, D. P., et al. (2017) · The Journal of Cell Biology · doi:10.1083/jcb.201612160 · Cited by 241
Zhang, J., Zong, W., Hong, W., Zhang, Z.-T., & Wang, Y. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.03.007 · Cited by 241
Application of CRISPR-Cas9 genome editing technology in various fields: A review
Ansori, A. N. M., Antonius, Y., Susilo, R. J. K., Hayaza, S., Kharisma, V. D., Parikesit, A. A., Zainul, R., Jakhmola, V., Saklani, T., Ребезов, М. Б., et al. (2023) · Narra J · doi:10.52225/narra.v3i2.184 · Cited by 243
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
Yin, H., Song, C.-Q., Suresh, S., Kwan, S.-Y., Wu, Q., Walsh, S., Ding, J., Bogorad, R. L., Zhu, L. J., Wolfe, S. A., et al. (2018) · Nature Chemical Biology · doi:10.1038/nchembio.2559 · Cited by 240
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Pradeepa, M. M., Grimes, G. R., Kumar, Y., Olley, G., Taylor, G. C. A., Schneider, R., & Bickmore, W. A. (2016) · Nature Genetics · doi:10.1038/ng.3550 · Cited by 240
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Wong, A. S. L., Choi, G. C. G., Cui, C. H., Pregernig, G., Milani, P., Adam, M., Perli, S. D., Kazer, S. W., Gaillard, A., Hermann, M., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1517883113 · Cited by 240
NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation
Lahaye, X., Gentili, M., Silvin, A., Conrad, C., Picard, L., Jouve, M. J., Zueva, E., Maurin, M., Nadalin, F., Knott, G. J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.08.062 · Cited by 240
Xu, J., van Herwijnen, Z., Dräger, D. B., Sui, C., Haring, M. A., & Schuurink, R. C. (2018) · The Plant Cell · doi:10.1105/tpc.18.00571 · Cited by 240
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Turchiano, G., Andrieux, G., Klermund, J., Blattner, G., Pennucci, V., el Gaz, M., Monaco, G., Poddar, S., Mussolino, C., Cornu, T. I., et al. (2021) · Cell stem cell · doi:10.1016/j.stem.2021.02.002 · Cited by 240
Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., & Kawahara, A. (2015) · Scientific Reports · doi:10.1038/srep08841 · Cited by 239
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Yin, K., Han, T., Liu, G., Chen, T., Wang, Y., Yu, A. Y. L., & Liu, Y. (2015) · Scientific Reports · doi:10.1038/srep14926 · Cited by 239
Continuous genetic recording with self-targeting CRISPR-Cas in human cells
Perli, S. D., Cui, C. H., & Lu, T. K. (2016) · Science · doi:10.1126/science.aag0511 · Cited by 239
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma
Chow, R. D.-W., Guzman, C. D., Wang, G., Schmidt, F., Youngblood, M. W., Ye, L., Errami, Y., Dong, M. B., Martinez, M. A. Q., Zhang, S., et al. (2017) · Nature Neuroscience · doi:10.1038/nn.4620 · Cited by 240
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Rajagopal, N., Srinivasan, S., Kooshesh, K., Guo, Y., Edwards, M. D., Banerjee, B., Syed, T., Emons, B. J. M., Gifford, D. K., & Sherwood, R. I. (2016) · Nature Biotechnology · doi:10.1038/nbt.3468 · Cited by 238
Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
Qin, P., Parlak, M., Kuscu, C., Bandaria, J. N., Mir, M., Szlachta, K. A., Singh, R., Darzacq, X., Yıldız, A., & Adli, M. (2017) · Nature Communications · doi:10.1038/ncomms14725 · Cited by 238
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Chen, W., Zhang, Y. P., Zhang, Y., Pi, Y., Gu, T., Song, L., Wang, Y., & Ji, Q. (2018) · iScience · doi:10.1016/j.isci.2018.07.024 · Cited by 238
Kim, H. K., Kim, Y., Lee, S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, S. (2019) · Science Advances · doi:10.1126/sciadv.aax9249 · Cited by 238
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Papapetrou, E. P., & Schambach, A. (2016) · Molecular Therapy · doi:10.1038/mt.2016.38 · Cited by 238
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Wang, Z., Pan, Q., Gendron, P., Zhu, W., Guo, F., Cen, S., Wainberg, M. A., & Liang, C. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.03.042 · Cited by 237
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Cobb, J. N., Biswas, P. S., & Platten, J. D. (2018) · Theoretical and Applied Genetics · doi:10.1007/s00122-018-3266-4 · Cited by 236
Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria
Farasat, I., Kushwaha, M., Collens, J., Easterbrook, M., Guido, M., & Salis, H. M. (2014) · Molecular Systems Biology · doi:10.15252/msb.20134955 · Cited by 235
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Tchieu, J., Zimmer, B., Fattahi, F., Amin, S., Zeltner, N., Chen, S., & Studer, L. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.08.015 · Cited by 235
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Matsuura, T., Baek, M. L., Kwon, J., & Hong, C. I. (2015) · Fungal Biology and Biotechnology · doi:10.1186/s40694-015-0015-1 · Cited by 235
CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
Li, H., Shen, C. R., Huang, C.-H., Sung, L., Wu, M., & Hu, Y. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.09.006 · Cited by 235
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Liu, X., Wu, S., Xu, J., Sui, C., & Wei, J. (2017) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2017.01.002 · Cited by 235
Long noncoding RNA Bmncr regulates mesenchymal stem cell fate during skeletal aging
Li, C., Xiao, Y., Yang, M., Su, T., Sun, X. H., Guo, Q., Huang, Y., & Luo, X. (2018) · Journal of Clinical Investigation · doi:10.1172/jci99044 · Cited by 235
CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation
Han, X., Liu, Z., Jo, M. C., Zhang, K., Li, Y., Zeng, Z., Li, N., Zu, Y., & Qin, L. (2015) · Science Advances · doi:10.1126/sciadv.1500454 · Cited by 235
Shen, C., Que, Z., Xia, Y., Tang, N., Li, D., He, R., & Cao, M. (2017) · Journal of Plant Biology · doi:10.1007/s12374-016-0400-1 · Cited by 234
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Hess, G. T., Tycko, J., Yao, D., & Bassik, M. C. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.029 · Cited by 234
Nucleosome breathing and remodeling constrain CRISPR-Cas9 function
Isaac, R. S., Jiang, F., Doudna, J. A., Lim, W. A., Narlikar, G. J., & Almeida, R. (2016) · eLife · doi:10.7554/elife.13450 · Cited by 234
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Liu, H., Jian, L., Xu, J., Zhang, Q., Zhang, M., Jin, M., Peng, Y., Yan, J., Han, B., Liu, J., et al. (2020) · The Plant Cell · doi:10.1105/tpc.19.00934 · Cited by 234
A CRISPR view of developmentPreprint
Harrison, M. M., Jenkins, B., O’Connor-Giles, K. M., & Wildonger, J. (2014) · Genes & Development · doi:10.1101/gad.248252.114 · Cited by 234
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Lowder, L. G., Zhou, J., Zhang, Y., Malzahn, A., Zhong, Z., Hsieh, T.-F., Voytas, D. F., Zhang, Y., & Qi, Y. (2018) · Molecular Plant · doi:10.1016/j.molp.2017.11.010 · Cited by 232
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Barrangou, R. (2013) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.1159 · Cited by 232
Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice
Xu, R., Li, H., Qin, R., Wang, L., Li, L., Wei, P., & Yang, J. (2014) · Rice · doi:10.1186/s12284-014-0005-6 · Cited by 232
Oceguera-Yañez, F., Kim, S., Matsumoto, T., Tan, G. W., Xiang, L., Hatani, T., Kondo, T., Ikeya, M., Yoshida, Y., Inoue, H., et al. (2015) · Methods · doi:10.1016/j.ymeth.2015.12.012 · Cited by 232
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Schuster, M., Schweizer, G., Reißmann, S., & Kahmann, R. (2015) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2015.09.001 · Cited by 231
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Xu, T., Li, Y., Shi, Z. J., Hemme, C. L., Li, Y., Zhu, Y., Nostrand, J. D. V., He, Z., & Zhou, J. Z. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.00873-15 · Cited by 231
Use of designer nucleases for targeted gene and genome editing in plants
Weeks, D. P., Spalding, M. H., & Yang, B. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12448 · Cited by 231
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Inui, M., Miyado, M., Igarashi, M., Tamano, M., Kubo, A., Yamashita, S., Asahara, H., Fukami, M., & Takada, S. (2014) · Scientific Reports · doi:10.1038/srep05396 · Cited by 230
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Charpentier, E., & Doudna, J. A. (2013) · Nature · doi:10.1038/495050a · Cited by 229
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
Karvelis, T., Gasiūnas, G., Young, J. K., Bigelyte, G., Šilanskas, A., Cigan, M., & Šikšnys, V. (2015) · Genome biology · doi:10.1186/s13059-015-0818-7 · Cited by 229
Varshney, G. K., Carrington, B., Pei, W., Bishop, K. S., Chen, Z., Fan, C., Xu, L., Jones, M. S., LaFave, M. C., Ledin, J., et al. (2016) · Nature Protocols · doi:10.1038/nprot.2016.141 · Cited by 228
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Chung, J., Wu, X., Lambert, T. J., Lai, Z. W., Walther, T. C., & Farese, R. V. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.10.006 · Cited by 229
Weissman, D. (2015) · Expert Review of Vaccines · doi:10.1586/14760584.2015.973859 · Cited by 228
CRMAGE: CRISPR Optimized MAGE Recombineering
Ronda, C., Pedersen, L. E., Sommer, M. O. A., & Nielsen, A. T. (2016) · Scientific Reports · doi:10.1038/srep19452 · Cited by 228
Jasin, M., & Haber, J. E. (2016) · DNA repair · doi:10.1016/j.dnarep.2016.05.001 · Cited by 229
Retinal stem cell transplantation: Balancing safety and potential
Singh, M. S., Park, S. S., Albini, T. A., Canto-Soler, M. V., Klassen, H. J., MacLaren, R. E., Takahashi, M., Nagiel, A., Schwartz, S. D., & Bharti, K. (2019) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2019.100779 · Cited by 228
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Metadata
- Authors
- Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M.
- Affiliations
- Harvard University (US)
- Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
- Boston University (US)
- Corresponding author
- Mali, Prashant
- Yang, Luhan
- Church, George M.
- Venue
- Science
- Published
- 2013-02-15
- Volume
- 339
- Issue
- 6121
- Pages
- 823-826
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3712628
- OpenAlex
- W2003171404
- Cited by
- 9,210
- References
- 46
- Citations by year
- 2026: 286 · 2025: 408 · 2024: 518 · 2023: 568 · 2022: 727 · 2021: 789 · 2020: 795 · 2019: 813 · 2018: 864 · 2017: 863 · 2016: 884 · 2015: 697 · 2014: 680 · 2013: 307
- FWCI
- 383.86
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- RECERCAT (Consorci de Serveis Universitaris de Catalunya) (acceptedVersion)
- Repositori digital de la UPF (Universitat Pompeu Fabra) (submittedVersion)
- Funders
- National Human Genome Research Institute (P50HG005550)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- CRISPR/Cas9, guide RNA, human genome editing, AAVS1, multiplex genome editing, induced pluripotent stem cells, K562 cells, HEK293T cells, RNA-guided nucleases
- MeSH terms
- Chromosomes, Human, Pair 19, Codon, Exons, Genetic Engineering, Humans, RNA, Genome, Human, Gene Targeting, K562 Cells, DNA Cleavage, Inverted Repeat Sequences, Genetic Loci, Induced Pluripotent Stem Cells, Clustered Regularly Interspaced Short Palindromic Repeats
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