Genomic Diversity across Candida auris Clinical Isolates Shapes Rapid Development of Antifungal Resistance In Vitro and In Vivo

Burrack, Laura S.; Todd, Robert T.; Soisangwan, Natthapon; Wiederhold, Nathan P.; Selmecki, Anna

doi:10.1128/mbio.00842-22

Cited by 82Antifungal resistance and susceptibilityFungal and yeast genetics researchFungal Plant Pathogen Control

Genomic Diversity across Candida auris Clinical Isolates Shapes Rapid Development of Antifungal Resistance In Vitro and In Vivo

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Burrack, Laura S., Todd, Robert T., Soisangwan, Natthapon, Wiederhold, Nathan P., Selmecki, Anna·2022·mBio

DOI 10.1128/mbio.00842-22PMC PMC9426540

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Abstract

Drug resistant Candida auris infections are recognized by the CDC as an urgent threat. Here, we obtained and characterized a set of clinical isolates of C. auris including multiple isolates from the same patient.

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In vitro evolution of C. auris during fluconazole exposure is relatively slow compared to C. albicans and mainly composed of SNPs, with aneuploidy a minority effect.
“In vitro evolution during fluconazole exposure is relatively slow, compared to C. albicans, and mainly composed of a single nucleotide polymorphism (SNP), with aneuploidy a minority effect [135,136].”
From Insight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance (2025) · cites this paper as [136]
The Discussion (‘Multiple mechanisms of resistance and tolerance’; page unavailable) says most mutations during fluconazole evolution were SNVs and a minority of isolates acquired aneuploidies. It contrasts these mechanisms with those in C. albicans, but does not establish that C. auris evolves more slowly; the Results instead describe rapid acquisition of mutations in C. auris. (LLM, conf=0.88)
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Self-citation
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Authors: Burrack, L. S., Todd, R. T., Soisangwan, N., Wiederhold, N. P., & Selmecki, A.
Affiliations: University of Minnesota (US)
The University of Texas at San Antonio Health Science Center (US)
Corresponding author: Selmecki, Anna
ORCID: Burrack, Laura S.
Todd, Robert T.
Soisangwan, Natthapon
Wiederhold, Nathan P.
Selmecki, Anna
Venue: mBio
Publisher: American Society for Microbiology
Published: 2022-08-30
Volume: 13
Issue: 4
Pages: e0084222-e0084222
ISSN: 2150-7511 (ISSN-L)
DOI: 10.1128/mbio.00842-22
PMC: PMC9426540
OpenAlex: W4283817659
Cited by: 82
References: 79
Citations by year: 2026: 21 · 2025: 25 · 2024: 20 · 2023: 14 · 2022: 2
FWCI: 7.44
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
journals.asm.org/non-commercial-tdm-license (tdm)
Locations: mBio (publishedVersion, cc-by)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: HHS | National Institutes of Health (R01AI143689)
Burroughs Wellcome Fund (1020388)
Topics: Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
Keywords: Candida auris, antifungal resistance, fluconazole resistance, genomic diversity, clinical isolates, drug resistance evolution, resistance stability, mutation rate, multidrug resistance, fungal genomics
MeSH terms: Candida auris, Antifungal Agents, Candida, Humans, Microbial Sensitivity Tests, Fluconazole, Genomics, Drug Resistance, Fungal

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Genomic Diversity across Candida auris Clinical Isolates Shapes Rapid Development of Antifungal Resistance In Vitro and In Vivo

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