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Cited by 309Cardiovascular and exercise physiologyMuscle metabolism and nutritionSports Performance and Training

High-intensity aerobic interval training increases fat and carbohydrate metabolic capacities in human skeletal muscle

Perry, Christopher G.R., Heigenhauser, George J.F., Bonen, Arend, Spriet, Lawrence L.·2008·Applied Physiology Nutrition and Metabolism
DOI 10.1139/h08-097
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Abstract

High-intensity aerobic interval training (HIIT) is a compromise between time-consuming moderate-intensity training and sprint-interval training requiring all-out efforts. However, there are few data regarding the ability of HIIT to increase the capacities of fat and carbohydrate oxidation in skeletal muscle. Using untrained recreationally active individuals, we investigated skeletal muscle and whole-body metabolic adaptations that occurred following 6 weeks of HIIT (~1 h of 10 x 4 min intervals at ~90% of peak oxygen consumption (VO2 peak), separated by 2 min rest, 3 d.week-1). A VO2 peak test, a test to exhaustion (TE) at 90% of pre-training VO2 peak, and a 1 h cycle at 60% of pre-training VO2 peak were performed pre- and post-HIIT. Muscle biopsies were sampled during the TE at rest, after 5 min, and at exhaustion. Training power output increased by 21%, and VO2 peak increased by 9% following HIIT. Muscle adaptations at rest included the following: (i) increased cytochrome c oxidase IV content (18%) and maximal activities of the mitochondrial enzymes citrate synthase (26%), beta-hydroxyacyl-CoA dehydrogenase (29%), aspartate-amino transferase (26%), and pyruvate dehydrogenase (PDH; 21%); (ii) increased FAT/CD36, FABPpm, GLUT 4, and MCT 1 and 4 transport proteins (14%-30%); and (iii) increased glycogen content (59%). Major adaptations during exercise included the following: (i) reduced glycogenolysis, lactate accumulation, and substrate phosphorylation (0-5 min of TE); (ii) unchanged PDH activation (carbohydrate oxidation; 0-5 min of TE); (iii) ~2-fold greater time during the TE; and (iv) increased fat oxidation at 60% of pre-training VO2 peak. This study demonstrated that 18 h of repeated high-intensity exercise sessions over 6 weeks (3 d.week-1) is a powerful method to increase whole-body and skeletal muscle capacities to oxidize fat and carbohydrate in previously untrained individuals.

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  • Factors such as intensity and duration of exercise, training status, diet and sex have a profound effect on the utilisation of substrates during exercise.

    “factors such as intensity and duration of exercise [138], training status [139], diet [135] and sex [140] have a profound effect on the utilisation of substrates during exercise.”

    From Regulation of Energy Substrate Metabolism in Endurance Exercise (2021) · cites this paper as [138]

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Authors
Perry, C. G. R., Heigenhauser, G. J. F., Bonen, A., & Spriet, L. L.
Affiliations
University of Guelph (CA)
ORCID
Venue
Applied Physiology Nutrition and Metabolism
Publisher
Canadian Science Publishing
Published
2008-12
Volume
33
Issue
6
Pages
1112-1123
ISSN
  • 1715-5312 (ISSN-L)
  • 1715-5320
DOI
10.1139/h08-097
OpenAlex
W2120556768
Cited by
309
References
58
Citations by year
2026: 8 · 2025: 13 · 2024: 18 · 2023: 18 · 2022: 19 · 2021: 18 · 2020: 24 · 2019: 23 · 2018: 19 · 2017: 20 · 2016: 19 · 2015: 25 · 2014: 21 · 2013: 21 · 2012: 15
FWCI
27.37
Type
journal article
Language
en
Open access
closed
Licence
www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations
  • Applied Physiology Nutrition and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
Keywords
fat oxidation, carbohydrate oxidation, skeletal muscle metabolism, mitochondrial enzymes, FAT/CD36, GLUT4, monocarboxylate transporters, glycogen, pyruvate dehydrogenase, cytochrome c oxidase, peak oxygen uptake, training effects, untrained individuals
MeSH terms
Adult, Biopsy, Blood Glucose, Citrate (si)-Synthase, Electron Transport Complex IV, Physical Exertion, Fats, Female, Glycerol, Glycogen, Humans, Male, Physical Endurance, Pyruvate Dehydrogenase Complex, Blotting, Western, Biomarkers, Exercise, Muscle, Skeletal, Lactic Acid, Aspartate Aminotransferase, Mitochondrial, Carbohydrate Metabolism, Young Adult

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