Cited by 215Adipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and MetabolismCardiovascular Disease and Adiposity

Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Implication of nonadipose cells in adipose tissue

Bruun, Jens M., Lihn, Aina S., Madan, Atul K., Pedersen, Steen B., Schiøtt, Kirsten M., Fain, John N., Richelsen, Bjørn·2004·American Journal of Physiology-Endocrinology and Metabolism

Abstract

IL-8 is released from human adipose tissue. Circulating IL-8 is increased in obese compared with lean subjects and is associated with measures of insulin resistance, development of atherosclerosis, and cardiovascular disease. We studied 1) the production and release of IL-8 in vitro from paired samples of subcutaneous (SAT) and visceral (VAT) adipose tissue and 2) the production of IL-8 from whole adipose tissue, isolated adipocytes, and nonfat cells of adipose tissue. IL-8 release from VAT was fourfold higher than from SAT ( P < 0.05), and IL-8 mRNA was twofold higher in VAT compared with SAT ( P < 0.01). Dexamethasone (50 nM) attenuated IL-8 production by 50% ( P < 0.05), and IL-1β (2 μg/l) increased IL-8 production up to 15-fold ( P < 0.001). IL-8 release from whole SAT explants correlated with body mass index (BMI; r = 0.78; P < 0.001), as did IL-8 release from nonfat cells ( r = 0.79; P < 0.001). However, no correlation was found between IL-8 release from the fraction of isolated adipocytes and BMI ( r = 0.01). In conclusion, we demonstrated an increased release of IL-8 from VAT compared with SAT. Furthermore, our data suggest that the observed elevation in circulating levels of IL-8 in obese subjects is due primarily to the release of IL-8 from nonfat cells from adipose tissue. The high levels of IL-8 release from human adipose tissue and accumulation of this tissue in obese subjects may account for some of the increase in circulating IL-8 observed in obesity.

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Crossref · 10.1152/ajpendo.00269.2003

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  • L. salivarius UCC118 at 10^10 CFU per day for 21 days did not affect serum IL-1α, IL-1β, IL-4, sIL-2R, sIL-6R, TNF-α or IFN-γ concentrations in healthy adults aged 20-65 years.

    “L. salivarius UCC118 (10 10 CFU/d for 21 d) did not affect serum IL-1a, IL-1b, IL-4, sIL-2R, sIL-6R, TNF-a or IFN-g concentrations in healthy adults aged 20 -65 years (829) .”

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Authors
Bruun, J. M., Lihn, A. S., Madan, A. K., Pedersen, S. B., Schiøtt, K. M., Fain, J. N., & Richelsen, B.
Affiliations
Aarhus University (DK)
Venue
American Journal of Physiology-Endocrinology and Metabolism
Published
2004-01
Volume
286
Issue
1
Pages
E8-E13
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
OpenAlex
W2125302795
Cited by
215
References
31
Citations by year
2026: 4 · 2025: 5 · 2024: 9 · 2023: 7 · 2022: 3 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 7 · 2017: 12 · 2016: 6 · 2015: 7 · 2014: 11 · 2013: 12 · 2012: 14
FWCI
9.82
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
Sundhed og Sygdom, Det Frie Forskningsråd
Topics
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
Keywords
interleukin-8, visceral adipose tissue, subcutaneous adipose tissue, obesity, adipocytes, IL-8 mRNA expression, dexamethasone, IL-1β, body mass index, insulin resistance
MeSH terms
Adipose Tissue, Adult, Aerosols, Cell Differentiation, Cells, Cultured, Connective Tissue, Female, Fusarium, Humans, Middle Aged, Obesity, Morbid, RNA, Messenger, Stem Cells, Viscera, Body Mass Index, Interleukin-8, Stromal Cells, Adipocytes, Subcutaneous Tissue, Depsipeptides, In Vitro Techniques

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