Cited by 263Forensic Toxicology and Drug AnalysisCholesterol and Lipid MetabolismAnalytical Chemistry and Chromatography

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Human cecal bile acids: concentration and spectrum

Hamilton, James P., Xie, Guofeng, Raufman, Jean-Pierre, Hogan, Susan, Griffin, Terrance L., Packard, Christine A., Chatfield, Dale A., Hagey, Lee R., Steinbach, Joseph H., Hofmann, Alan F.·2007·American Journal of Physiology-Gastrointestinal and Liver Physiology

DOI 10.1152/ajpgi.00027.2007

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Abstract

To obtain information on the concentration and spectrum of bile acids in human cecal content, samples were obtained from 19 persons who had died an unnatural death from causes such as trauma, homicide, suicide, or drug overdose. Bile acid concentration was measured via an enzymatic assay for 3α-hydroxy bile acids; bile acid classes were determined by electrospray ionization mass spectrometry and individual bile acids by gas chromatography mass spectrometry and liquid chromatography mass spectrometry. The 3α-hydroxy bile acid concentration (μmol bile acid/ml cecal content) was 0.4 ± 0.2 mM (mean ± SD); the total 3-hydroxy bile acid concentration was 0.6 ± 0.3 mM. The aqueous concentration of bile acids (supernatant after centrifugation) was identical, indicating that most bile acids were in solution. By liquid chromatography mass spectrometry, bile acids were mostly in unconjugated form (90 ± 9%, mean ± SD); sulfated, nonamidated bile acids were 7 ± 5%, and nonsulfated amidated bile acids (glycine or taurine conjugates) were 3 ± 7%. By gas chromatography mass spectrometry, 10 bile acids were identified: deoxycholic (34 ± 16%), lithocholic (26 ± 10%), and ursodeoxycholic (6 ± 9), as well as their primary bile acid precursors cholic (6 ± 9%) and chenodeoxycholic acid (7 ± 8%). In addition, 3β-hydroxy derivatives of some or all of these bile acids were present and averaged 27 ± 18% of total bile acids, indicating that 3β-hydroxy bile acids are normal constituents of cecal content. In the human cecum, deconjugation and dehydroxylation of bile acids are nearly complete, resulting in most bile acids being in unconjugated form at submicellar and subsecretory concentrations.

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Johnson, L. R. (2006) · Elsevier eBooks · doi:10.1016/b978-0-12-088394-3.x5000-4 · Cited by 4,616
Bile salt biotransformations by human intestinal bacteria
Ridlon, J. M., Kang, D.-J., & Hylemon, P. B. (2006) · Journal of Lipid Research · doi:10.1194/jlr.r500013-jlr200 · Cited by 2,829
Measurement of gastrointestinal pH profiles in normal ambulant human subjects
Evans, D. F., Pye, G., Bramley, R., Clark, A. G., Dyson, T. J., & Hardcastle, J. D. (1988) · Gut · doi:10.1136/gut.29.8.1035 · Cited by 1,240
Bile Salt Transporters
Meier, P. J., & Stieger, B. (2002) · Annual Review of Physiology · doi:10.1146/annurev.physiol.64.082201.100300 · Cited by 594
Colonic Secretion of Water and Electrolytes Induced by Bile Acids: Perfusion Studies in Man
Mekhjian, H. S., Phillips, S. F., & Hofmann, A. F. (1971) · Journal of Clinical Investigation · doi:10.1172/jci106644 · Cited by 584
Self-citation
Role of Bile Acid Malabsorption in Pathogenesis of Diarrhea and Steatorrhea in Patients with Ileal Resection
Hofmann, A. F., & Poley, J. R. (1972) · Gastroenterology · doi:10.1016/s0016-5085(72)80109-4 · Cited by 549
Self-citation
Role of bile acid malabsorption in pathogenesis of diarrhea and steatorrhea in patients with ileal resection. I. Response to cholestyramine or replacement of dietary long chain triglyceride by medium chain triglyceride.
Hofmann, A. F., & Poley, J. R. (1972) · PubMed · Cited by 477
Self-citation
THE FUNCTION OF BILE SALTS IN FAT ABSORPTION. THE SOLVENT PROPERTIES OF DILUTE MICELLAR SOLUTIONS OF CONJUGATED BILE SALTS
Hofmann, A. (1963) · Biochemical Journal · doi:10.1042/bj0890057 · Cited by 353
Self-citation
High pressure liquid chromatographic analysis of conjugated bile acids in human bile: simultaneous resolution of sulfated and unsulfated lithocholyl amidates and the common conjugated bile acids
Rossi, S. S., Converse, J. L., & Hofmann, A. F. (1987) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38684-3 · Cited by 316
Self-citation
Characterization of the kinetics of the passive and active transport mechanisms for bile acid absorption in the small intestine and colon of the rat
Schiff, E. R., Small, N. C., & Dietschy, J. M. (1972) · Journal of Clinical Investigation · doi:10.1172/jci106931 · Cited by 303

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Hang, S., Paik, D., Yao, L., Kim, E., Trinath, J., Lu, J., Ha, S., Nelson, B. N., Kelly, S. P., Wu, L., et al. (2019) · Nature · doi:10.1038/s41586-019-1785-z · Cited by 1,457
Bile acids and the gut microbiota: metabolic interactions and impacts on disease
Collins, S. L., Stine, J. G., Bisanz, J. E., Okafor, C. D., & Patterson, A. D. (2023) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00805-x · Cited by 1,214
Connecting dysbiosis, bile-acid dysmetabolism and gut inflammation in inflammatory bowel diseases
Duboc, H., Rajca, S., Rainteau, D., Benarous, D., Maubert, M.-A., Quervain, E., Thomas, G., Barbu, V., Humbert, L., Despras, G., et al. (2013) · Gut · doi:10.1136/gutjnl-2012-302578 · Cited by 930
Bacterial metabolism of bile acids promotes generation of peripheral regulatory T cells
Campbell, C., McKenney, P. T., Konstantinovsky, D., Isaeva, O. I., Schizas, M., Verter, J., Mai, C., Jin, W.-B., Guo, C.-J., Violante, S., et al. (2020) · Nature · doi:10.1038/s41586-020-2193-0 · Cited by 901
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Sinha, S. R., Haileselassie, Y., Nguyen, L. P., Tropini, C., Wang, M., Becker, L. S., Sim, D., Jarr, K. J., Bishop, E. S., Singh, G., et al. (2018) · Cell Host & Microbe · doi:10.1016/j.chom.2020.01.021 · Cited by 880
Dietary metabolism, the gut microbiome, and heart failure
Tang, W. H. W., Li, D. Y., & Hazen, S. L. (2019) · Nature Reviews Cardiology · doi:10.1038/s41569-018-0108-7 · Cited by 789
Bile Salts and Glycine as Cogerminants for Clostridium difficile Spores
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Intestinal transport and metabolism of bile acids
Dawson, P. A., & Karpen, S. J. (2015) · Journal of Lipid Research · doi:10.1194/jlr.r054114 · Cited by 598
Human gut bacteria produce ΤΗ17-modulating bile acid metabolites
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Metabolism of Cholesterol and Bile Acids by the Gut Microbiota
Gérard, P. (2013) · Pathogens · doi:10.3390/pathogens3010014 · Cited by 580
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Background
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Funabashi, M., Grove, T. L., Wang, M., Varma, Y., McFadden, M. E., Brown, L. C., Guo, C., Higginbottom, S., Almo, S. C., & Fischbach, M. A. (2020) · Nature · doi:10.1038/s41586-020-2396-4 · Cited by 590
Secondary bile acids and short chain fatty acids in the colon: a focus on colonic microbiome, cell proliferation, inflammation, and cancer
Zeng, H., Umar, S., Rust, B., Lazarova, D., & Bordonaro, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20051214 · Cited by 480
A biosynthetic pathway for a prominent class of microbiota-derived bile acids
Devlin, A. S., & Fischbach, M. A. (2015) · Nature Chemical Biology · doi:10.1038/nchembio.1864 · Cited by 448
Bile salts of vertebrates: structural variation and possible evolutionary significance
Hofmann, A. F., Hagey, L. R., & Krasowski, M. D. (2009) · Journal of Lipid Research · doi:10.1194/jlr.r000042 · Cited by 427
Self-citation
Bile Acid Sulfation: A Pathway of Bile Acid Elimination and Detoxification
Alnouti, Y. (2009) · Toxicological Sciences · doi:10.1093/toxsci/kfn268 · Cited by 404
The receptor TGR5 mediates the prokinetic actions of intestinal bile acids and is required for normal defecation in mice
Alemi, F., Poole, D. P., Chiu, J., Schoonjans, K., Cattaruzza, F., Grider, J. R., Bunnett, N. W., & Corvera, C. U. (2013) · Gastroenterology · doi:10.1053/j.gastro.2012.09.055 · Cited by 362
Bile acids in glucose metabolism and insulin signalling — mechanisms and research needs
Ahmad, T. R., & Haeusler, R. A. (2019) · Nature Reviews Endocrinology · doi:10.1038/s41574-019-0266-7 · Cited by 348
The enterohepatic circulation of bile acids in mammals: form and functions
Alan, F. (2009) · Frontiers in bioscience · doi:10.2741/3399 · Cited by 335
Bile acids modified by the intestinal microbiota promote colorectal cancer growth by suppressing CD8+ T cell effector functions
Cong, J., Liu, P., Han, Z., Ying, W., Li, C., Yang, Y., Wang, S., Yang, J., Cao, F., Shen, J., et al. (2024) · Immunity · doi:10.1016/j.immuni.2024.02.014 · Cited by 333
A bacterial bile acid metabolite modulates Treg activity through the nuclear hormone receptor NR4A1
Li, W., Hang, S., Fang, Y., Bae, S., Zhang, Y., Zhang, M., Wang, G., McCurry, M. D., Bae, M., Paik, D., et al. (2021) · Cell Host & Microbe · doi:10.1016/j.chom.2021.07.013 · Cited by 306
Ursodeoxycholic acid and lithocholic acid exert anti-inflammatory actions in the colon
Ward, J. B. J., Lajczak, N. K., Kelly, O. B., O’Dwyer, A. M., Giddam, A. K., Ní Gabhann, J., Franco, P., Tambuwala, M. M., Jefferies, C. A., Keely, S., et al. (2017) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00256.2016 · Cited by 286
A selective gut bacterial bile salt hydrolase alters host metabolism
Yao, L., Seaton, S. C., Ndousse-Fetter, S., Adhikari, A. A., DiBenedetto, N., Mina, A. I., Banks, A. S., Bry, L., & Devlin, A. S. (2018) · eLife · doi:10.7554/elife.37182 · Cited by 274
Guts and Gall: Bile Acids in Regulation of Intestinal Epithelial Function in Health and Disease
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Self-citation
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Self-citation
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Self-citation
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Self-citation
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Bile acids: short and long term effects in the intestine
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Development of a covalent inhibitor of gut bacterial bile salt hydrolases
Adhikari, A. A., Seegar, T. C. M., Ficarro, S. B., McCurry, M. D., Ramachandran, D., Yao, L., Chaudhari, S. N., Ndousse-Fetter, S., Banks, A. S., Marto, J. A., et al. (2020) · Nature Chemical Biology · doi:10.1038/s41589-020-0467-3 · Cited by 116
Lithocholic acid binds TULP3 to activate sirtuins and AMPK to slow down ageing
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Koh, E., Hwang, I. Y., Lee, H. L., Sotto, R. D., Lee, J. W. J., Lee, Y. S., March, J. C., & Chang, M. W. (2022) · Nature Communications · doi:10.1038/s41467-022-31334-z · Cited by 116
Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives byBacteroides intestinalisAM-1 isolated from human feces
Fukiya, S., 荒田美紀, Kawashima, H., Yoshida, D., Kaneko, M. A. N., Minamida, K., Watanabe, J., Ogura, Y., Uchida, K., Itoh, K., et al. (2009) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2009.01531.x · Cited by 113
Drug-Induced Perturbations of the Bile Acid Pool, Cholestasis, and Hepatotoxicity: Mechanistic Considerations beyond the Direct Inhibition of the Bile Salt Export Pump
Rodrigues, A. D., Lai, Y., Cvijic, M. E., Elkin, L. L., Zvyaga, T. A., & Soars, M. G. (2013) · Drug Metabolism and Disposition · doi:10.1124/dmd.113.054205 · Cited by 107
Fecal metabolome profiling of liver cirrhosis and hepatocellular carcinoma patients by ultra performance liquid chromatography–mass spectrometry
Cao, H., Huang, H., Xu, W., Chen, D., Yu, J., Li, J., & Li, L. (2011) · Analytica Chimica Acta · doi:10.1016/j.aca.2011.02.038 · Cited by 107
A biosynthetic pathway for the selective sulfonation of steroidal metabolites by human gut bacteria
Yao, L., D’Agostino, G. D., Park, J., Hang, S., Adhikari, A. A., Zhang, Y., Li, W., Ávila-Pacheco, J., Bae, S., Clish, C. B., et al. (2022) · Nature Microbiology · doi:10.1038/s41564-022-01176-y · Cited by 96
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Wise, J. L., & Cummings, B. P. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1093420 · Cited by 94
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Winston, J. A., Rivera, A. J., Cai, J., Thanissery, R., Montgomery, S. A., Patterson, A. D., & Theriot, C. M. (2020) · Infection and Immunity · doi:10.1128/iai.00045-20 · Cited by 91
Microbiome-mediated bile acid modification: Role in intestinal drug absorption and metabolism
Enright, E. F., Griffin, B. T., Gahan, C. G. M., & Joyce, S. A. (2018) · Pharmacological Research · doi:10.1016/j.phrs.2018.04.009 · Cited by 91
Immunology of bile acids regulated receptors
Fiorucci, S., Marchianò, S., Urbani, G., Giorgio, C. D., Distrutti, E., Zampella, A., & Biagioli, M. (2024) · Progress in Lipid Research · doi:10.1016/j.plipres.2024.101291 · Cited by 88
Acetylcholine-induced activation of M3 muscarinic receptors stimulates robust matrix metalloproteinase gene expression in human colon cancer cells
Xie, G., Cheng, K., Shant, J., & Raufman, J. (2009) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.90519.2008 · Cited by 84
Self-citation
Induction of farnesoid X receptor signaling in germ-free mice colonized with a human microbiota
Wahlstrom, A. M., Kovatcheva‐Datchary, P., Ståhlman, M., Khan, M.-T., Bäckhed, F., & Marschall, H. (2016) · Journal of Lipid Research · doi:10.1194/jlr.m072819 · Cited by 87
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Hang, S., Paik, D., Devlin, A. S., Trinath, J., Lu, J.-P., Ha, S., Nelson, B. N., Kelly, S. P., Wu, L., Zheng, Y., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/465344 · Cited by 84
Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine
Wen, J., Mercado, G. P., Volland, A., Doden, H. L., Lickwar, C. R., Crooks, T. A., Kakiyama, G., Kelly, C., Cocchiaro, J. L., Ridlon, J. M., et al. (2021) · Science Advances · doi:10.1126/sciadv.abg1371 · Cited by 85
A Rat Model of Chronic Postinflammatory Visceral Pain Induced by Deoxycholic Acid
Traub, R. J., Tang, B., Ji, Y., Pandya, S., Yfantis, H. G., & Sun, Y. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.08.051 · Cited by 83
UPLC-Q-TOF/HSMS/MSE-based metabonomics for adenine-induced changes in metabolic profiles of rat faeces and intervention effects of ergosta-4,6,8(14),22-tetraen-3-one
Zhao, Y., Li, Z., Long, F.-Y., Cheng, X., Bai, X., Wei, F., & Lin, R. (2012) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2012.12.002 · Cited by 82
Interplay of host microbiota, genetic perturbations, and inflammation promotes local development of intestinal neoplasms in mice
Bongers, G., Pacer, M. E., Geraldino, T. H., Chen, L., He, Z., Hashimoto, D., Furtado, G. C., Ochando, J., Kelley, K. A., Clemente, J. C., et al. (2014) · The Journal of Experimental Medicine · doi:10.1084/jem.20131587 · Cited by 79
Infection with Bacteroides Phage BV01 Alters the Host Transcriptome and Bile Acid Metabolism in a Common Human Gut Microbe
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Hofmann, A. F., Loening‐Baucke, V., Lavine, J. E., Hagey, L. R., Steinbach, J. H., Packard, C. A., Griffin, T. L., & Chatfield, D. A. (2008) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/mpg.0b013e31816920a6 · Cited by 75
Self-citation
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Mroz, M. S., Lajczak, N., Goggins, B. J., Keely, S., & Keely, S. J. (2018) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00435.2016 · Cited by 75
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Cuperus, F. J. C., Claudel, T., Gautherot, J., Halilbasic, E., & Trauner, M. (2014) · Drug Metabolism and Disposition · doi:10.1124/dmd.113.056358 · Cited by 76
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Authors: Hamilton, J. P., Xie, G., Raufman, J.-P., Hogan, S., Griffin, T. L., Packard, C. A., Chatfield, D. A., Hagey, L. R., Steinbach, J. H., & Hofmann, A. F.
Affiliations: University of Maryland, Baltimore (US)
Maryland Department of Agriculture (US)
San Diego State University (US)
University of California San Diego (US)
ORCID: Hamilton, James P.
Xie, Guofeng
Raufman, Jean-Pierre
Hogan, Susan
Chatfield, Dale A.
Venue: American Journal of Physiology-Gastrointestinal and Liver Physiology
Publisher: American Physiological Society
Published: 2007-07
Volume: 293
Issue: 1
Pages: G256-G263
ISSN: 0193-1857 (ISSN-L)
1522-1547
DOI: 10.1152/ajpgi.00027.2007
OpenAlex: W2124723056
Cited by: 263
References: 53
Citations by year: 2026: 19 · 2025: 17 · 2024: 23 · 2023: 14 · 2022: 24 · 2021: 27 · 2020: 21 · 2019: 17 · 2018: 18 · 2017: 6 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 8 · 2012: 12
FWCI: 16.70
Type: journal article
Language: en
Open access: closed
Locations: American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
PubMed (publishedVersion)
Funders: National Cancer Institute (CA107345, R01CA107345)
National Institute of Diabetes and Digestive and Kidney Diseases (DK64891, T32DK067872, R03DK064891)
Topics: Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
Physical Sciences › Chemistry › Analytical Chemistry › Analytical Chemistry and Chromatography
Keywords: bile acids, cecal contents, bile acid concentration, bile acid conjugation, deoxycholic acid, lithocholic acid, ursodeoxycholic acid, cholic acid, chenodeoxycholic acid, bile acid deconjugation
MeSH terms: Bile Acids and Salts, Cecum, Chenodeoxycholic Acid, Cholic Acids, Chromatography, Liquid, Deoxycholic Acid, Gastrointestinal Contents, Humans, Lithocholic Acid, Gas Chromatography-Mass Spectrometry, Ursodeoxycholic Acid, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry

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Human cecal bile acids: concentration and spectrum

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